Kuhlmann et al. Suppl. Figure 4 A bisulfite sequencing CG CHG CHH Δ open: unmethylated filled:...
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Kuhlmann et al. Suppl. Figure 4 A A tS N 1 A C T TA A T TA G C A C T C A A A T T A A A C A A A A TA A G T G G T G G T T G TA C A A G C C T A G T T T T A A T T C T A C G G A T C A G TA C T A A T C C TGAATG G A A A TTGACAT G T T T T T T T T T T T T G A A TA T C T G G A A G T T C A G G C C C A A A G G C C T TA C A T C T C C C A G A G G C G G G A C C C A TA C C A G A A A T T C A T C T TCTTTGGAAA A G A G A T C C T T C C C A A C G T G T C T C G A A T C C A G C A C C T C TA T C T G A G A G A T T TA C C A C T G G G C C A A C A A C A C GTTGGT G A A A TTGACAT A T T T T C T T T T T G T A G A T G A A A A A G G A A C T T C T T CTTATGTTTA Wt seedlings bisulfite sequencing CG CHG CHH Δ open: unmethylated filled: methylated
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Transcript of Kuhlmann et al. Suppl. Figure 4 A bisulfite sequencing CG CHG CHH Δ open: unmethylated filled:...
Kuhlmann et al. Suppl. Figure 4 A
A tSN 1
ACTTAATTAG CACTC AAATT AAACAAAATA AGTGG TG GTT GTAC AAG CCT AGTTTTAATT CTAC GGATCA GTACTAATCC TG AATG
GA AA TTGACATGTT TTTTTTTTTT GAATATC TGG AAGTTCAGG C CCAAAG GCCT TACATCTCCC AG AGGC GGG A CCCATACCAG AAATTCATC T TCTTTGG AAA
AGAGATCCTT CCCAACGTGT CTCGAATC CA GCACCTCTAT CTGAGAG ATT TACCACTG GG CCAACAACAC G TTGGTGA AA TTGACAT
ATT TTCTTTTTG T AG ATGAAAAA GG AACTTCTT CTTATG TTTA
W tseed lings
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 B
A tSN 1suvh2G K 516A 07seed lings
A C T TA AT TA G C A C T C A A ATT A A A C A A A ATA A G T G G T G G T T G TA C A A G C C T A G T TT TA ATT C TA C G G AT C A G TA C TA ATC C TG A AT G
GAAA TTGACATG T T T TT T TT T TT T G A ATATC T G G A A G TT C A G G C C C A A A G G C C T TA C ATC T C C C A G A G G C G G G A C C C ATA C C A G A A ATT C AT C T T C TT T G G A A A
A G A G AT C C TT C C C A A C G T G T C TC G A AT C C A G C A C C T C TAT C TG A G A G ATT TA C C A C TG G G C C A A C A A C A C G T TG G T GAAA TTGACAT
AT T TT C T TT T TG T A G AT G A A A A A G G A A C T TC T T C T TAT G T T TA
28957
234121097811165
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 C
ACTTAATTAG CACTCAAATT AAACAAAATA AGTGGTGGTT GTACAAGCCT AGTTTTAATT CTACGGATCA GTACTAATCC TGAATG
GAAA TTGACATGTT TTTTTTTTTT GAATATCTGG AAGTTCAGGC CCAAAGGCCT TACATCTCCC AGAGGCGGG A CCCATACCAG AAATTCATCT TCTTTGGAAA
AGAGATCCTT CCCAACGTGT CTCGAATCCA GCACCTCTAT CTGAGAGATT TACCACTGGG CCAACAACAC GTTGGT GAAA TTGACAT
ATT TTCTTTTTGT AG ATGAAAAA GGAACTTCTT CTTATGTTTA
A tSN 1suvh9S ALK 048033seed lings
2679111
123497861151210
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 D
A tSN 1suvh2 suvh9seed lings
A C TTA AT TA G C A C TC A AATT A A A C A A A ATA AG T G G T G G T T G TA C A A G C C T A G T TT TA ATT C TAC G G AT C A G TA C TA AT C C T G A AT G
G A A A TT G A C AT G T T T T TT T TT T TT G A ATAT C T G G AA G T TC A G G C C C AA A G G C C T TA C AT C T C C C A G A G G C G G G A C C C ATA C C A G A A AT TC ATC T T C T TT G G A A A
A G A G AT C C T T C C C AA C G T G T C T C G AATC C A G C A C C TC TAT C T G A G A G AT T TA C C A C T G G G C C A AC A A C A C G T T G G T G A A A TT G A C AT
AT T T T C TT T TT G T A G AT G A A A A A G G A AC TT C T T C T TAT G T TTA
29461 011381 2175
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 E
A tSN 1W tleaves
ACTTAATTAG CACTC AAATT AAACAAAATA AGTGG TG GTT GTAC AAG CCT AGTTTTAATT CTAC GGATCA GTACTAATCC TG AATG
GA AA TTGACATGTT TTTTTTTTTT GAATATC TGG AAGTTCAGG C CCAAAG GCCT TACATCTCCC AG AGGC GGG A CCCATACCAG AAATTCATC T TCTTTGG AAA
AGAGATCCTT CCCAACGTGT CTCGAATC CA GCACCTCTAT CTGAGAG ATT TACCACTG GG CCAACAACAC G TTGGTGA AA TTGACAT
ATT TTCTTTTTG T AG ATGAAAAA GG AACTTCTT CTTATG TTTA
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 F
ACTTAATTAG CACTCAAATT AAACAAAATA AGTGGTGGTT GTACAAGCCT AGTTTTAATT CTACGGATCA GTACTAATCC TGAATG
GAAA TTGACATGTT TTTTTTTTTT GAATATCTGG AAGTTCAGGC CCAAAGGCCT TACATCTCCC AGAGGCGGG A CCCATACCAG AAATTCATCT TCTTTGGAAA
AGAGATCCTT CCCAACGTGT CTCGAATCCA GCACCTCTAT CTGAGAGATT TACCACTGGG CCAACAACAC GTTGGT GAAA TTGACAT
ATT TTCTTTTTGT AG ATGAAAAA GGAACTTCTT CTTATGTTTA
A tSN 1suvh2G K 516A 07leaves
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 G
ACTTAATTAG CACTCAAATT AAACAAAATA AGTGGTGGTT GTACAAGCCT AGTTTTAATT CTACGGATCA GTACTAATCC TGAATG
GAAA TTGACATGTT TTTTTTTTTT GAATATCTGG AAGTTCAGGC CCAAAGGCCT TACATCTCCC AGAGGCGGG A CCCATACCAG AAATTCATCT TCTTTGGAAA
AGAGATCCTT CCCAACGTGT CTCGAATCCA GCACCTCTAT CTGAGAGATT TACCACTGGG CCAACAACAC GTTGGT GAAA TTGACAT
ATT TTCTTTTTGT AG ATGAAAAA GGAACTTCTT CTTATGTTTA
A tSN 1suvh9S ALK 048033leaves
34678910
12128643511710
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 H
A tSN 1suvh2 suvh9leaves
A C T TA AT TA G C A C T C A A AT T A A A C A A A ATA A G T G G T G G T T G TA C A A G C C T A G T T T TA AT T C TA C G G AT C A G TA C TA AT C C T G A AT G
G A A A TTG A C ATG T T T T T T T T T T T T G A ATAT C T G G A A G T T C A G G C C C A A A G G C C T TA C AT C T C C C A G A G G C G G G A C C C ATA C C A G A A AT T C AT C T T C T T T G G A A A
A G A G AT C C T T C C C A A C G T G T C T C G A AT C C A G C A C C T C TAT C T G A G A G AT T TA C C A C T G G G C C A A C A A C A C G T T G G T G A A A TTG A C AT
AT T T T C T T T T T G T A G AT G A A A A A G G A A C T T C T T C T TAT G T T TA
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 I
ACTTAATTAG CACTCAAATT AAACAAAATA AGTGGTGGTT GTACAAGCCT AGTTTTAATT CTACGGATCA GTACTAATCC TGAATG
GAAA TTGACATGTT TTTTTTTTTT GAATATCTGG AAGTTCAGGC CCAAAGGCCT TACATCTCCC AGAGG CGG GA CCCATACCAG AAATTCATCT TCTTTGGAAA
AGAGATCCTT CCCAACGTGT CTCGAATCCA GCACCTCTAT CTGAGAGATT TACCACTGGG CCAACAACAC GTTGG T GAAA TTGACAT
ATT TTCTTTTTGT AG ATGAAAAA GGAACTTCTT CTTATGTTTA
A tSN 1P ro35S -m ycSU VH 2leaves
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
A tC O PIA 4
GTGCTTTCCATGGCTCAGACCTTACACTCACAACAAATTAGAAGAACGATCGAGACGGTGTGTGTTCCTCGGTTCTCTTTAACTCAAACAGCCT
ACCTC TGTTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCGTGTTTGATG AAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTGAACAGAGCTCCTCGCCGTTAGTTACGCCCATACTCTCATCATCGTCG
W tseed lings po lyprote in
transposasegag
integrase
RdDP
analysed region
4651312
31 2647118
Kuhlmann et al. Suppl. Figure 4 J
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 K
A tC O PIA 4
GTGCTTTCCATG GCTCAGACCTTACACTCACAACAAATTAGAAGAACGATCGAGACG GTGTG TG TTCCTCG GTTCTCTTTAACTC AAACAGCCT
ACCTC TGTTTCGATGTTGAACATAAGCG ACTTTACACATCTCGCCATG CGTGTTTGATG AAG CCTC CTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCG TAACCTTTG AACAG AG CTC CTCG CCGTTAGTTACGC CCATACTCTCATCATC GTC G
suvh2seed lings po lyprote in
transposasegag
integrase
RdDP
analysed region
4651312
31 2647118
56912781013114
148267351
81 29467235111 0
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 L
A tC O PIA 4
GTGCTTTCCATG GCTCAGACCTTACACTCACAACAAATTAGAAGAACGATCGAGACG GTGTG TG TTCCTCG GTTCTCTTTAACTC AAACAGCCT
ACCTC TGTTTCGATGTTGAACATAAGCG ACTTTACACATCTCGCCATG CGTGTTTGATG AAG CCTC CTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCG TAACCTTTG AACAG AG CTC CTCG CCGTTAGTTACGC CCATACTCTCATCATC GTC G
suvh9seed lings po lyprote in
transposasegag
integrase
RdDP
analysed region
4651312
31 2647118
56912781013114
14826735 bisulfite sequencing
CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 M
A tC O PIA 4
GTGCTTTCCATGGCTCAGACCTTACACTCACAACAAATTAGAAGAACGATCGAGACGGTGTGTGTTCCTCGGTTCTCTTTAACTCAAACAGCCT
ACCTC TGTTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCGTGTTTGATG AAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTGAACAGAGCTCCTCGCCGTTAGTTACGCCCATACTCTCATCATCGTCG
suvh2 suvh9seed lings po lyprote in
transposasegag
integrase
RdDP
analysed region
4651312
31 2647118
5789
741035162
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 N
A tC O PIA 4
GTGCTTTCCATGGCTCAGACCTTACACTCACAACAAATTAG AAGAACGATCGAGACGGTGTGTGTTCCTCGG TTCTCTTTAACTCAAACAGCCT
ACCTCTG TTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCGTGTTTG ATGAAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTGAACAGAGCTCCTCGCCG TTAGTTACG CCCATACTCTCATCATCGTCG
W tleaves po lyprotein
transposasegag
integrase
RdDP
analysed reg ion
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 O
A tC O PIA 4 suvh2 leaves
GTGCTTTCCATG GCTCAGACCTTACACTCACAACAAATTAGAAGAACGATCGAGACGGTGTGTGTTCCTCGGTTCTCTTTAACTCAAACAGCCT
ACCTCTGTTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCGTGTTTGATGAAGCCTCCTTTC CCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCG TAACCTTTGAACAGAGCTCCTCGCCGTTAGTTACGCCCATACTCTCATCATCGTCG
SuvH2-KO Gabi-kat 516A07
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 P
A tC O PIA 4
GTGCTTTCCATGGCTCAGACCTTACACTCACAACAAATTAG AAGAACGATCGAGACGGTGTGTGTTCCTCGG TTCTCTTTAACTCAAACAGCCT
ACCTCTG TTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCGTGTTTG ATGAAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTGAACAGAGCTCCTCGCCG TTAGTTACG CCCATACTCTCATCATCGTCG
suvh9leaves po lyprotein
transposasegag
integrase
RdDP
analysed reg ion
147851036121192
351098116
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 Q
A tC O PIA 4
GTGCTTTCCATG GCTCAGACCTTACACTCACAACAAATTAGAAG AACGATCGAG AC GGTGTGTGTTCCTCGG TTCTCTTTAACTCAAACAG CCT
ACCTCTGTTTCGATGTTGAACATAAGCGACTTTACACATCTCGCCATGCG TG TTTGATGAAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTG AACAG AGCTCCTCG CCGTTAGTTACG CCCATACTCTCATCATCGTCG
po lyprotein
transposasegag
integrase
RdDP
analysed reg ion
suvh2 suvh9 leaves
359107
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
Kuhlmann et al. Suppl. Figure 4 R
GTGCTTTCCATG GCTCAGACCTTACACTCACAACAAATTAG AAG AACGATCGAG ACGG TGTGTGTTCCTCG GTTCTCTTTAACTCAAACAGCCT
ACCTCTG TTTCGATGTTG AACATAAGC GACTTTACACATCTCGCCATGCG TG TTTG ATGAAGCCTCCTTTCCCTTCTCCAACCTCACATCCCAAA
ATTCTCTCCCCACCGTAACCTTTGAAC AG AGCTCCTCG CCGTTAG TTACG CCCATACTCTCATCATCG TCG
A tC O PIA 4 P ro35S -m ycSU VH 2 leaves
bisulfite sequencing CG CHG CHH
Δopen: unmethylatedfilled: methylated
