Innate Lymphoid Cell (ILC) Lineage Pathway and … Innate Lymphoid Cell (ILC) Lineage Pathway and...
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RnDSy-lu-2945
Innate Lymphoid Cell (ILC) Lineage Pathway and Localization
Adipose Tissue of Mesentery
Cross section of Small Intestine
IL-2IL-12
ETS-1PU.1
IL-15
IL-7
GATA-3
Ikaros
IL-12IL-18
PerforinGranzyme
CD56dim
CD16+
CD117/c-kit– IFN-γTNF-αIL-10IL-13
IL-4IL-5IL-9IL-13Amphiregulin
IL-25IL-33TSLP
CD25/IL-2 Rα+
CD45+
CD90/Thy1+
CD117/c-kit+
CD127/IL-7 Rα+
CD161/NKRP1A+
IL-17 RB+
ST2/IL-33 R+
TSLP R+
CRTH-2+
ICOS+
Sca-1+
KLRG1+
GATA-3high
IL-7
RORαGATA-3
ID2Notch
IL-7
IL-7
IFN-γ
IL-2IL-12
IL-17IL-22IFN-γ
IL-23IL-1β
IL-17IL-22LTα/β
CD4+
CD7+
CD90/Thy1+
CD117/c-kithigh
CD127/IL-7 Rα+
CD161/NKRP1A+
CXCR5+
CXCR6+
CCR6+
IL-1 RI+
IL-2 Rα+
IL-23 R+
RORγthigh
RORγtAHRRUNX1
RORγtAhRT-bet
IL-23IL-1β
IL-2IL-23
NKp44–
NKp46–
CD25/IL-2 Rα+
CD56+
CD90/Thy1+
CD117/c-kithigh
CD127/IL-7 Rα+
Sca-1+
IL-1R+
IL-12 Rβ1+
IL-23 R+
CCR6–
T-betlow
RORγthigh
NKp44+
NKp46+
CD25/IL-2 Rαlow
CD44+
CD56+
CD90/Thy1+
CD117/c-kitlow
CD127/IL-7 Rαlow
CD161/NKRP1A+
IL-12 Rβ1high
Sca-1+
IL-1 R+
IL-2 Rβ+
IL-23 R+
CCR6–
T-bethigh
RORγtlow
IFN-γIL-22
T-bethigh
T-bethigh
T-betlow
T-betlow
CD127/IL-7 Rα+
Integrin α4β7+
ID2+
PLZF–
T-betEOMESNFIL3/E4BP4
IL-12IL-15
IFN-γPerforinGranzyme
IFN-γ
T-bet
Integrin α4β7+
CD25/IL-2 Rα–
CD27+
C1q R1/CD93–
CD117/c-kit+
2B4/CD244+
CD127/IL-7 Rα+
Sca-1+
IL-2 Rβ–
PLZFhigh
NKp44–
CD16–
CD34–
CD56–
CD69–/+
CD94–
CD117/c-kit–
CD127/IL-7 Rα+
CD161/NKRP1A+
CXCR3+
CCL3+
IL-1 R+
IL-12 Rβ2+
IL-15 Rα–
ICOS+
T-bet+
NKp44+
NKp46+
CD9+
CD56+
CD39+
CD69+
CD101/IGSF2+
CD103/Integrin αE+ (human)IL-2 Rβ+
CD127/IL-7 Rα+ (human)CD160+ (mouse)CD161/NKRP1A+
Integrin α4β7+
CXCR6+
IL-12 Rβ1+
IL-12 Rβ2+
IL-15 Rα+
T-bet+
IL-12IL-18
NKp44–
CD25/IL-2 Rα+
CD56–
CD117/c-kit–
CD127/IL-7 Rα+
IL-2 Rβ+
T-bethigh
RORγtlow
CD56bright
CD16dim/–
CD117/c-kit+
T-betNFIL3/E4BP4
Mesentery ofSmall Intestine
NKP
CLP
CLP
iILC2iNK
mNK
Cytotoxic NK cell
ImmunoregulatoryNK cell
ILC2
IntraepithelialILC1 ILC1
ILC1
ILCP
LTiP LTi
NCR+
ILC3
NCR–
ILC3
CD7high
CD8+
CD34+
IL-2 Rβ+
CD161/NKRP1A+
CD7high
CD34–
CD56+
CD117/c-kitlow
CD127/IL-7 Rα–
CD161/NKRP1A+
IL-2 Rβ+NKp46+
Integrin αM/CD11b+
Fcγ RIII/CD16+
CD25/IL-2 Rα+
CD56+
CD90/Thy1+
CD127/IL-7 Rα+
CD161/NKRP1A+
NKG2D/CD314+
KIR/CD158+
IL-2 Rβ+
IL-12 Rβ2+
Tunica Muscularis
Tunica Submucosa
Muscularis Mucosa
Intestinal Lamina Propria
Intestinal Epithelium
Intraepithelial ILC1
Dendritic Cell
Intestinal Villi
ILC1
Natural Killer cell (NK)
Macrophage
Peyer’s Patch
NCR+/–ILC3
Group 1NKP, iNK, mNK, Cytotoxic NK cell, Immunoregulatory NK cell, ILC1, Intraepithelial ILC1
Group 2iILC2, ILC2
Group 3LTiP, LTi, NCR+ILC3, NCR–ILC3
Lymphoid Tissue inducer cell (LTi)
ILC Groups
Fat-associatedlymphoid cluster
ILC2 Localization
KeyCLP: Common Lymphoid ProgenitorNKP: NK Cell PrecursoriNK: immature NK cellmNK: mature NK cellILCP: Innate Lymphoid Cell ProgenitorLTiP: Lymphoid Tissue inducer ProgenitorLTi: Lymphoid Tissue inducer cellNCR: Natural Cytotoxicity Triggering ReceptoriILC2: immature ILC2
Transcription factors are indicated in bold font.
NOTE: This poster conveys a general overview and should be considered neither comprehensive nor definitive. The details of this information are understood to be subject to interpretation. © R&D Systems 2016
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Learn more | rndsystems.com/ilcidentification
Innate lymphoid cells (ILCs) are important effectors and regulators involved in immune surveillance and the preservation of tissue integrity. Consistent with the suggestion that ILCs are involved in the early detection of pathogens, ILCs are present at mucosal surfaces and respond to molecules secreted from the epithelium. They are derived from a common lymphoid progenitor cell (CLP) that originates from fetal liver or adult bone marrow. Sequentially expressed transcription factors orchestrate the differentiation of the CLP into either an LTi progenitor cell (LTiP), an NK progenitor cell (NKP), or an ILC progenitor (ILCP). Because ILCs share developmental and functional similarities with helper T (Th) cells, a nomenclature for ILCs has been established based on Th cell classification.
ILCs are categorized into three groups according to the transcription factors mediating their development and the cytokines they secrete. Predominantly localized in the intestinal lamina propria (LTi cells, NCR-ILC3, NK cells and ILC1) and epithelium (Intraepithelial ILC1), group 1 and group 3 ILCs mediate the balance between symbiotic microbiota and the intestinal immune system and are implicated in the response against pathogenic gut bacteria. Group 2 ILCs have been described as part of fat-associated lymphoid clusters in human and mouse mesentery, and are normally present in human and mouse airway mucosa and skin.
