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B cell p15 CD4 T cell p16 CD8 T cell p17 NK/ILC p18 NKT/γδ T cell p19 Macrophage / Monocyte p20 Dendritic cell p21 Granulocyte p22 Megakaryocyte / Platelet p23 Stem cell p24 Other cell types p25 B cell p4 CD4 T cell p5 CD8 T cell p6 NK/ILC p7 NKT/γδ T cell p8 Macrophage / Monocyte p9 Dendritic cell p10 Granulocyte p11 Megakaryocyte / Platelet p12 Stem cell p13 Other cell types p14 CLP Common Lymphoid Progenitor CMP Common Myeloid Progenitor HSC Hematopoietic Stem Cell ILC Innate Lymphoid Cell LTi Lymphoid Tissue inducer M1 classically activated M2 alternatively activated NK Natural Killer Tfh Follicular Helper T cell Th1 T Helper Type 1 cell Th2 T Helper Type 2 cell Treg Regulatory T cell

Transcript of CLP Common Lymphoid Progenitor - WORK-FLOW · 2020. 2. 11. · CLP Common Lymphoid Progenitor CMP...

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B cell p15 CD4 T cell p16 CD8 T cell p17 NK/ILC p18 NKT/γδ T cell p19 Macrophage / Monocyte p20 Dendritic cell p21 Granulocyte p22 Megakaryocyte / Platelet p23 Stem cell p24 Other cell types p25

B cell p4 CD4 T cell p5 CD8 T cell p6 NK/ILC p7 NKT/γδ T cell p8 Macrophage / Monocyte p9 Dendritic cell p10Granulocyte p11Megakaryocyte / Platelet p12Stem cell p13Other cell types p14

CLP Common Lymphoid ProgenitorCMP Common Myeloid Progenitor HSC Hematopoietic Stem Cell ILC Innate Lymphoid Cell LTi Lymphoid Tissue inducer M1 classically activatedM2 alternatively activatedNK Natural Killer Tfh Follicular Helper T cell Th1 T Helper Type 1 cell Th2 T Helper Type 2 cell Treg Regulatory T cell

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Table of Contents
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Abbreviations
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Canonical Human & Mouse Cell Types
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Human
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Mouse
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Disclaimer
Christine
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The content provided in this guide, assembled in good faith by WORK-FLOW, is adapted from Thermo Fisher Scientific's Immune Cell Guide from 2017 and is for research use only and not for use in diagnostic procedures. WORK-FLOW assumes no responsibility or liability for any errors or omissions in content which is provided with no guarantees of completeness, accuracy, or usefulness. The user should take all necessary steps to ascertain that the information provided within this guide has been verified in the specie(s) and tissue(s) of interest.
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Hematopoiesis
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B cell markers

Human

Follicular

SurfaceCD20CD21CD22CD27CD23h�CD24CD40hCD45R (B220)CD69hCD80hCD81CD86hCD137 (4-1BB)hCD275 (B7-H2)CD279 (PD-1)CD360 (IL-2 receptor)HLA-DRIgDhigh�

IgMlow/int

Intracellular/transcription factorBcl-6EBF1FoxO1IkarosPax5

Marginal zone

SurfaceCD1dCD9CD21�CD45R (B220)CD81CD103CD180 (RP105)CD268 (BAFF receptor)�CD307d (FcRL4)IgM

Intracellular/transcription factorEBF1Notch2Pax5

Memory

SurfaceCD27IgGTLR1, 2, 6, 7, 10

Intracellular/transcription factorCBF1Pax5Spi-B

Germinal center

SecretedIgAIgEIgG

SurfaceCD45R (B220)CD81

Intracellular/transcription factorAIDBach2Bcl-6�IRF8NF-kappaBPax5

Plasma cell

SecretedIgAIgEIgG

SurfaceCD9CD19low/neg

CD20low/neg

CD27CD31CD38CD81CD138�CD184 (CXCR4)CD252 (OX40 ligand)CD269 (BCMA)

Intracellular/transcription factorBLIMP1IRF4XBP-1

Pan markers

CD19 CD20 CD22CD70hCD79α/β Igκ/λ

4

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Tfh

SecretedIL-21

SurfaceCD183 (CXCR3) CD185 (CXCR5)� CD278 (ICOS)� CD279 (PD-1)�

Intracellular/transcription factorBATFBcl-6c-MafIRF4STAT3

Treg

SecretedIL-10TGFβ

SurfaceCD25�CD39CD73CD101CD121aCD121b CD137 (4-1BB)CD152 (CTLA-4)CD357 (GITR/AITR)GARPhLAPhTIGIT

Intracellular/transcription factorc-RelEosFoxp3�HeliosSTAT5

Th1

SecretedIFNγIL-2 TNFαTNFβ (LTα)

SurfaceCD94CD119 (IFNγR1)CD183 (CXCR3)�CD186 (CXCR6)CD191 (CCR1)CD195 (CCR5)CD212 (IL-12Rβ1)CD218a (IL-18Rα)CD254 (RANKL)CD366 (TIM3)

Intracellular/transcription factorSTAT1STAT4T-bet�

Th17

SecretedGM-CSFIL-17AIL-17AFIL-17FIL-21IL-22

SurfaceCD121aCD161CD194 (CCR4)CD196 (CCR6)� CD360 (IL-21R)CD212 (IL-12β1)IL-23R

Intracellular/transcription factorAHRBATFc-MafIκBζ IRF4RORαRORγt�STAT3

Th2

SecretedAmphiregulin IL-4IL-5 IL-13

SurfaceCD184 (CXCR4)CD194 (CCR4)CD198 (CCR8)CD294 (CRTH2)�CD365 (TIM1)IL-25R (IL-17RB)IL-33R (ST2)

Intracellular/transcription factorBATF GATA-3�IRF4STAT6

Pan markers

CD2CD3CD4CD5CD7CD25hCD27CD28CD45RA (naïve)CD45RO (memory)CD62L (naïve: high, effector: low, memory: high)CD69hCD127 (IL7Rα)CD134 (OX40)hCD137 (4-1BB)hCD152 (CTLA-4)hCD154 (CD40L)hCD272 (BTLA)hCD279 (PD-1)h

CD4 T cell markers

Human

5

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CD8 T cell markers

Human

Central memory

SecretedIFNγint

IL-2int

TNFα int

SurfaceCCR7low

CD27CD28CD45ROCD62L�CD62Llow

CD127 (IL7Rα)high

CD197 (CCR7)low�

Intracellular/transcription factorEomesT-betint

Effector memory

SecretedGranzyme BIFNγhigh

IL-2low

PerforinTNFαhigh

SurfaceCD44CD45RO�CD62Llow

CD127 (IL7Rα)high

CD197 (CCR7)low

KLRG1�KRG1high

Intracellular/transcription factorEomesint

T-betint

Effector

SecretedCCL3 (MIP-1α)CCL4 (MIP-1β)CCL5 (RANTES)Granzyme AGranzyme BGranzyme KIFNγIL-2PerforinTNFα

SurfaceCD25hCD69hCD30CD122h�CD137 (4-1BB)CD134 (OX40)CD223 (LAG-3)CD272 (BTLA)CD278 (ICOS)CD279 (PD-1)CD366 (TIM3)KLRG1�

Intracellular/transcription factorT-bet

Naïve

SurfaceCD27CD45RACD62LCD127 (IL7Rα)CD197 (CCR7)

Pan markers

CD2CD3CD5CD7CD8

CD25hCD27CD28

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ILC3

SecretedIL-17AIL-17AFIL-17FIL-22

SurfaceCD56CD117�CD127 (IL-7Rα)�CD335 (NKp46)CD336 (NKp44)CD337 (NKp30)IL-23R Linneg

Intracellular/transcription factorAHRId2RORγtT-betTCF1

LTi

SecretedIL-17IL-22LTβTNFβ (LTα)

SurfaceCD7CD25CD117�CD127 (IL-7Rα)CD161CD196 (CCR6)CD252 (OX40L)IL-23RMHCII

Intracellular/transcription factorAHRId2RORγtRunx1TOX

ILC2

SecretedIL-4IL-5IL-9IL-13Amphiregulin

SurfaceCD25CD45CD117�CD127 (IL-7Rα)CD161CD184 (CXCR4)CD186 (CXCR6)CD199 (CCR9) CD278 (ICOS) CD294 (CRTH2)IL-25R (IL-17RB)IL-33R (ST2)Linneg

TSLP receptor

Intracellular/transcription factorArginase-1GATA-3Gfi1Id2RORαTCF1

ILC1

SecretedCCL3 (MIP-1α)IFNγ

SurfaceCD56CD103CD183 (CXCR3)CD335 (NKp46)CD336 (NKp44)CD337 (NKp30)Linneg

Intracellular/transcription factorId2Tbet

NK

SecretedGranulysinGranzyme AGranzyme BGranzyme KGranzyme M

IFNγIL-10PerforinTNFα

SurfaceCD11bCD16CD56�CD69hCD94CD122CD161CD244�CD226 CD314 (NKG2D)CD335 (NKp46)CD336 (NKp44) CD337 (NKp30)CD158 family KLRG1TIGIT

Intracellular/transcription factorE4BP4EomesGATA-3Id2Runx1T-betTOX

NK/ILC markers

Human

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Human

γδ T cell

SecretedGM-CSFGranzyme BIFNγIL-4IL-5IL-17APerforinTNFα

SurfaceCD5CD16CD27CD28CD45RACD56CD57CD62LCD69CD70hCD107aCD314 (NKG2D)TCR γδ�TCR Vδ1TCR Vδ2TCR Vδ3

NKT

SecretedIL-4hIL-10hIL-13hIFNγhTNFαh

SurfaceCD4CD8CD16CD56CD69CD160TCR Vα24�

Intracellular/transcription factorPLZFId2

Pan markers

CD3

NKT/γδ T cell markers

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Human

Macrophage/monocyte markers

Human

M2 macrophage

SecretedIDOIL-10TGFβ

SurfaceCD115CD204CD163CD206 (MMR)CD209 (DC-SIGN)FcεR1VSIG4

Intracellular/transcription factorIRF4STAT6

M1 macrophage

SecretedIFNγIL-1αIL-1βIL-6IL-12IL-23TNFα

SurfaceCD16CD16/CD32CD32CD64CD68CD80CD86CD369 (Dectin-1) Mer (MerTK)MHCII

Intracellular/transcription factorIRF5STAT1

Monocyte

SurfaceCD14�CD33CD172a (SIRPα)

Tissue associated macrophage

SurfaceAxlCD192 (CCR2)CD14CD68CD115CD163CD206CD369 (Dectin-1)HLA-DRCD273 (PD-L2)

IntracellularNOS2

Pan markers

CD11bCD15CD33CD40CD63CD68 (mature)CD80hCD85CD86hCD105hCD115CD169CD195 (CCR5)CD282 (TLR2)CD284 (TLR4)CD354 (Trem-1)CXCL10CXCL11CXCL9GPNMBMature macrophage markerMIP-2α (CXCL2)

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Dendritic cell markers

Human

Conventional dendritic cell

SecretedIDOIL-1βIL-6IL-8IL-12IL-15IL-23

SurfaceCD1bCD1c (BDCA-1)CD11c�CD14iCD40hCD49dCD141 (BDCA-3)CD172a (SIRPa)CD197 (CCR7)CD205 (DEC-205)CD207 (Langerin)CD206CD273 (B7-DC)CD282 (TLR2)CD284 (TLR4)CD369 (Dectin-1)CD370 (CLEC9A)

Intracellular/transcription factorCD135CD289 (TLR9)Fit3

Plasmacytoid dendritic cell

SecretedIFNαIFNβ

SurfaceCD85g (ILT7)CD123�CD283 (TLR3)CD303 (BDCA-2)�CD304 (BDCA-4)�CD370 (CLEC9A)

Intracellular/transcription factorCD287 (TLR7)CD289 (TLR9)E2-2IRF8

Pan markers

CD11bCD13CD33CD80hCD83CD86hMHCIIh

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Granulocyte markersMast

SecretedCathepsinsHistamineTNFαIL-4TGFßNGF

SurfaceCD9CD15CD24CD35CD43CD64CD116CD117 (c-kit)�CD123CD125CD126FcεR1�IL-33R (ST-2)

Neutrophil

SecretedElastaseLactoferrinIL-6IL-12TNFαIL-1 α/ß

SurfaceCD10CD15CD17CD24CD35CD43CD66aCD66b�CD66cCD66dCD89CD93CD112 CD114CD116CD157

CD177CD181 (CXCR1)CD282 (TLR2)CD284 (TLR4)CD286 (TLR6)Calprotectin (S100A8/A9)

Intracellular/transcription factorCD281 (TLR1)CD289 (TLR9)

Basophil

SecretedIL-4IL-13HistamineCCL3 (MIP-1α)

SurfaceBasophil MarkerCD9CD11aCD13CD16CD25CD33CD38CD43CD88CD123�CD125CD154CD192CD203cCD218 (IL-18R)CD282 (TLR2)

CD284 (TLR4)CD286 (TLR6)CD294FcεR1�

Intracellular/transcription factorC/EBPαCD281 (TLR1)CD289 (TLR9)GATA2

Eosinophil

SecretedMBPsEDNEPX

SurfaceCD9CD15CD24CD35CD43CD64CD116CD123CD125�CD126CD193 (CCR3)�CD244FcεR1

Pan markers

CD11bCD13CD15CD32CD33

Human

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Megakaryocyte/platelet markers

Human

Platelet

SurfaceCD9CD23CD31CD36CD41CD42a�CD42b�CD42cCD42dCD49fCD51CD60aCD61CD84CD92

CD107ah

CD107bhCD110CD147CD151CD173CD226GARPLAP

Megakaryocyte

SurfaceCD41�CD42aCD42b�CD42cCD42dCD49fCD51CD61CD110CD112CD123

12

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HumanHuman

Stem cell markersHSC

SurfaceCD38low/neg

CD59CD133�CD135 (Flt3)CD338Linneg

Intracellular/transcription factorGATA-3TdT

CMP

SurfaceCD33�CD45RACD123low

CD131CD135 (Flt3)CD173CD174Linneg

Intracellular/transcription factorIkarosPU.1

CLP

SurfaceCD7CD38CD45RACD90low

CD110CD117 (c-kit)low

CD127 (IL-7Rα)�HLA-DRLinneg

Intracellular/transcription factorAiolosc-mybGATA-3PU.1TdT

Pan markers

CD34CD117 (c-kit)CD164

13

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Human

CD24 CD49f CD66a CD75 CD104 CD121a CD133 CD167a CD326 (EpCAM) CytokeratinMHCII pos

Erythrocytes

CD45neg

CD51 CD61 CD235a/glycophorin

Fibroblasts

CD10CD29CD47CD81CD91CD121aCD140a/PDGFRaCD140b/PDGFRbVimentin

Neurons

CD6 CD24 CD90 CD143CD166 CD171 CD200 CD230 (prion protein) CD271 (NGFR) CD304 (neuropilin) Nestin

Stromal cells

CD10 CD34 CD157 Podoplanin/p38 Stro-1

Other cell type markers

Human

Epithelial cells

Endothelial cells

SurfaceCD31CD34CD54 (ICAM-1)CD61 (Integrinβ3)CD62E (E-selectin)CD105 (Endoglin)CD106 (VCAM-1)CD144 (VE-cadherin)CD146CD202b (Tie2)CD309 (FLK1)Podoplanin

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h = Upregulated i = Downregulated � = Key marker = Subset

Follicular

SurfaceCD20CD21/CD35low/neg

CD22CD23�CD24 (HSA)low

CD25hCD36hCD40hCD69hCD80hCD81CD86hCD180 (RP105)CD252 (OX40 ligand)hIgDhigh�

IgMlow/int

Intracellular/transcription factorBcl-6EBF1FoxO1IkarosPax5

Marginal zone

SurfaceCD1dCD5low

CD9CD21/CD35high�

CD23low/neg

CD24 (HSA)low

CD36CD81CD180 (RP105)CD363 (S1PR1)FcRL3IgDlow/neg

IgMhigh�

Intracellular/transcription factorEBF1Notch2

Germinal center

SurfaceCD19CD81CD95GL7�IgG

Intracellular/transcription factorAIDBach2Bcl-6IRF8NF-кBPax5

Plasma cell

SurfaceCD9CD16/32CD19low

CD81CD138�IgG

Intracellular/transcription factorBLIMP1IRF4XBP-1

Pan markers

CD20CD21/CD35low/neg

CD22CD23CD24 (HSA)low

CD25hCD36hCD40hCD69hCD80hCD81CD86hCD180 (RP105)

CD252 (OX40 ligand)hIgDhigh

IgMlow/int

Mouse

Memory

SurfaceCD38�CD45R (B220)low

CD267 (TACI)CD269 (BCMA)IgG

Intracellular/transcription factorCBF1Spi-B

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B cell markers

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Tfh

SecretedIL-21

SurfaceCD185 (CXCR5)� CD278 (ICOS)� CD279 (PD-1)�

Intra/TxnBATFBcl-6c-MafIRF4STAT3

Treg

SecretedIL-10TGFβ

SurfaceCD25 CD137 (4-1BB)CD39CD73CD101CD121aCD121bCD152 (CTLA-4)CD304 (Neuropilin-1)�CD357 (GITR)FR4GARPhLAPhTIGIT

Intracellular/transcription factorc-RelEosFoxp3�HeliosSTAT5

Th1

SecretedIFNγIL-2 TNFαTNFβ (LTα)

SurfaceCD94CD119 (IFNγR1)CD183 (CXCR3)�CD186 (CXCR6)CD191 (CCR1)CD195 (CCR5)CD212 (IL-12Rβ1)CD218a (IL-18Rα)CD254 (RANKL)CD366 (TIM3)

Intracellular/transcription factorSTAT1STAT4T-bet�

Th17

SecretedGM-CSFIL-17AIL-17AFIL-17FIL-21IL-22

SurfaceCD194 (CCR4)CD196 (CCR6)�CD121a CD212 (IL-12β1)CD360 (IL-21R)IL-23R

Intracellular/transcription factorAHRBATFc-MafIκBζ IRF4RORαRORγt�STAT3

Th2

SecretedAmphiregulin IL-4IL-5IL-10 IL-13

SurfaceCD184 (CXCR4)CD194 (CCR4)CD198 (CCR8)CD294 (CRTH2)CD365 (TIM1)IL-25R (IL-17RB)IL-33R (ST2)�

Intracellular/transcription factorBATF GATA-3�IRF4STAT6

Pan markers

CD3

CD4

CD5

CD7

CD25h

CD27

CD28

CD44h

CD62L (naïve: high, effector: low, memory: high)

CD69h

CD127 (IL7Rα)

CD134 (OX40)h

CD137 (4-1BB)h

CD152 (CTLA-4)h

CD154 (CD40L)h

CD272 (BTLA)h

CD278 (ICOS)h

CD279 (PD-1)h

Mouse

h = Upregulated i = Downregulated � = Key marker = Subset

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CD4 T cell markers

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Central memory

SecretedIL-4low

IFNγlow

SurfaceCD44CD62L�CD127 (IL-7Rα) CD197 (CCR7)high�

Intracellular/transcription factorbcl-6 Eomesint

T-betint

Effector memory

SecretedGranzyme BIFNγIL-2PerforinTNFα

SurfaceCD44CD57KLRG1�

Intracellular/transcription factorEomesint

T-betint

BLIMP1

Effector

SecretedCCL3 (MIP-1α)CCL4 (MIP-1β)CCL5 (RANTES)Granzyme AGranzyme BIFNγIL-2PerforinTNFα

SurfaceCD25hCD30CD44CD69hCD122hCD134 (OX40)CD137 (4-1BB)CD223 (LAG-3)CD272 (BTLA-4)CD278 (ICOS)CD279 (PD-1)CD366 (TIM3)KLRG1

Intracellular/transcription factorBLIMP1Id2T-bet

Naïve

SurfaceCD62LCD127 (IL-7Rα)CD197 (CCR7)CD183 (CXCR3)

Pan markers

CD3CD5CD8CD27CD28

Mouse

17 h = Upregulated i = Downregulated � = Key marker = Subset

CD8 T cell markers

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Mouse

ILC3

SecretedIL-17AIL-17AFIL-17FIL-22

SurfaceCD117CD127 (IL-7Rα)CD196 (CCR6)�CD335 (NKp46)IL-23RLinneg

Intracellular/transcription factorAHR�Id2RORγtT-betTCF1

LTi

SecretedIL-17LTβTNFβ (LTα)

SurfaceCD4CD25CD117CD127 (IL-7Rα)�CD185 (CXCR5)CD186 (CXCR6)CD196 (CCR6)IL-23RMHCII

Intracellular/transcription factorAHRId2RORγt�Runx1TOX

ILC2

SecretedAmphiregulinIL-4IL-5IL-9IL-13

SurfaceCD25CD117CD127 (IL-7Rα)CD184 (CXCR4)CD186 (CXCR6)CD199 (CCR9)CD278 (ICOS)CD294 (CRTH2)�IL-25R (IL-17RB)�LinnegLy-6A/E (Sca-1)IL-33R (ST2)TSLP receptor

Intracellular/transcription factorArginase-1GATA-3Gfi1Id2RORα�TCF1

ILC1

SecretedIFNγCCL3 (MIP-1α)

SurfaceCD103CD183 (CXCR3)CD335 (NKp46)�Linneg

Ly-6A/E (Sca-1)

Intracellular/transcription factorId2Tbet

NK

SecretedGranzyme A Granzyme BGranzyme KIFNγ

IL-10PerforinTNFα

SurfaceCD11bCD16CD25CD49b (DX5)�CD94CD122CD161c (NK1.1)CD226CD314 (NKG2D)CD335 (NKp46)�Ly-49 familyTIGIT

Intracellular/transcription factorE4BP4EomesGATA-3Id2Runx1T-betTOX

NK/ILC markers

18 h = Upregulated i = Downregulated � = Key marker = Subset

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Mouse

γδ T cell

SecretedGranzyme BIFNγIL-4IL-5IL-17APerforinTNFα

SurfaceCD25hCD27CD28CD44CD45R (B220)hCD45RBCD62LCD69hCD71hCD103CD122CD127 (IL-7Rα)CD150CD196 (CCR6)TCR γδ �

IL-1R1NK1.1 (on C57BL/6)TCR Vγ1TCR Vγ4TCR Vγ5TCR Vγ6TCR Vγ7

Intracellular/transcription factorEomes RORγtT-bet

NKT

SecretedIFNγhIL-4hIL-10hIL-13hIL-17hIL-21hIL-22hTNFαh

SurfaceCD4CD8CD44hCD49bCD94CD160CD183 (CXCR3)CD184 (CXCR4)CD186 (CXCR6)CD314 (NKG2D)DX5Ly-49NK1.1 (on C57BL/6)TCR Vα14�

Intracellular/transcription factorPLZFEgr2HEBHobit

Pan markers

CD3

19 h = Upregulated i = Downregulated � = Key marker = Subset

NKT/γδ T cell markers

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M2 macrophage

SecretedArginaseIDOIL-10TGFβYM1

SurfaceCD14CD115CD163CD204CD206 (MMR)CD209 (DC-SIGN)CSF1RFcεR1Ly-6C

Intracellular/transcription factorIRF4RELMα STAT6

M1 macrophage

SecretedIFNγIL-1IL-6IL-12IL-23TNFα

SurfaceCD14 CD16/CD32CD32CD64CD68CD80CD86CD204CD369 (Dectin-1)Ly-6CMer (MerTK)MHCII

Intracellular/transcription factorIRF5NOS2STAT1

Monocyte

SurfaceLy6cCSF1R

Pan markers

AxlCD11bCD16/CD32CD40CD64CD68CD107 (Mac3)CD115CD282 (TLR2)CD284 (TLR4)F4/80Galectin-3 (Mac2)GITRLGPNMBLy6G (Gr-1)MHC Class IIh

Mouse

Tissue associated macrophage

SurfaceAxlCD192 (CCR2)CD14CD68CD115CD163CD206CD369 (Dectin-1)HLA-DRCD273 (PD-L2)

IntracellularNOS2

20 h = Upregulated i = Downregulated � = Key marker = Subset

Macrophage/monocyte markers

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Conventional dendritic cell

SecretedIDOIL-1βIL-6IL-8IL-12IL-15IL-23

SurfaceCD4CD8 CD11blow

CD40hCD83CD197 (CCR7)CD205 (DEC-205)�CD207 (Langerin)CD209 (DC-SIGN)CD273 (B7-DC)CD369 (Dectin-1)CD370 (CLEC9A)DCIR2

Intracellular/transcription factorCD135 (Flt3)CD183 (TLR3)CD289 (TLR9)

Plasmacytoid dendritic cell

SecretedIFNαIFNβ

SurfaceCD45R (B220)CD317 (BST2, PDCA-1)�Siglec-H�

Intracellular/transcription factorCD287 (TLR7)CD289 (TLR9)E2-2IRF8

Pan markers

CD11cCD80hCD86hMHCIIh

Mouse

21 h = Upregulated i = Downregulated � = Key marker = Subset

Dendritic cell markers

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Mouse

Mast

SecretedCathepsinsHistamineTNFαIL-4TGFßNGF

SurfaceCD9CD15CD24CD35CD43CD64CD116CD117 (c-kit)�CD123CD125CD126FcεR1�IL-33R (ST2)

Neutrophil

SecretedElastaseLactoferrinIL-6IL-12TNFαIL-1 α/ß

SurfaceCD10CD15CD17CD24CD35CD43CD66a�CD66bCD66dCD89CD93 CD112 (Nectin-2)CD114 (G-CSFR)CD116CD157CD177CD181 (CXCR1)CD282 (TLR2)CD284 (TLR4)CD286 (TLR6)Ly-6G (Gr-1)�

Intracellular/transcription factorCD281 (TLR1)CD289 (TLR9)

Basophil

SecretedIL-4IL-13HistamineCCL3 (MIP-1α)

SurfaceCD9CD11aCD13CD25CD33CD38CD43CD88 (C5a receptor)CD123CD125CD154 (CD40 ligand)CD192 (CCR2)CD218 (IL-18 receptor)CD282 (TLR2)CD284 (TLR4)CD286 (TLR6)CD294 (CRTH2)FcεR1�

Intracellular/transcription factorCD281 (TLR1)CD289 (TLR9)C/EBPαGATA-2

Eosinophil

Secreted EPXMBPs

SurfaceCD9CD15CD24CD35 CD43 CD64CD116CD123CD125CD126CD170 (SiglecF)�CD193 (CCR3)�CD244FcεR1

Pan markers

CD11bCD16/CD32

22 h = Upregulated i = Downregulated � = Key marker = Subset

Granulocyte markers

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Platelet

SurfaceCD9CD23 CD41CD42a, b, and cCD43CD44CD47CD49bCD49fCD51 CD61CD63CD69CD93 CD62PhCD107ah CD107bhCD110CD147CD151GARPLAP

Megakaryocyte

SurfaceCD9 CD31 CD41� CD42b CD51 CD61 CD62PhCD93CD147CD151CD154GARPLAP

Mouse

23 h = Upregulated i = Downregulated � = Key marker = Subset

Megakaryocyte/platelet markers

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Mouse

HSC

SurfaceCD34CD38low/neg

CD59CD117 (c-kit)�CD133 (prominin-1)CD135 (Flt3)CD150 (SLAM)�Linneg

Ly-6A/E (Sca-1)�

Intracellular/transcription factorGATA-2TdT

CMP

SurfaceCD34CD123 (IL-3R)�Linneg

Intracellular/transcription factorIkarosPU.1

CLP

SurfaceCD34CD43CD135 (Flt3)CD127 (IL-7Rα)�Linneg

Intracellular/transcription factorAiolosc-mybGATA-3PU.1TdT

24 h = Upregulated i = Downregulated � = Key marker = Subset

Stem cell markers

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Mouse

Epithelial cells

CD24 CD49f CD66a CD75 CD104 CD121a CD133 CD167a CD326 (EpCAM) CytokeratinMHCII pos

Erythrocytes

CD24CD45neg

CD51 CD61 Ter119

Fibroblasts

CD10CD29CD47CD81CD91CD121aCD140a/PDGFRaCD140b/PDGFRbVimentin

Neurons

CD6 CD24 CD90 CD143CD166 CD171 CD200 CD230 (prion protein) CD271 (NGFR) CD304 (neuropilin) Nestin

Stromal cells

CD10 CD34 CD157 Podoplanin/p38 Stro-1

SurfaceCD31 (PECAM-1)CD34CD54 (ICAM-1)CD61 (Integrinβ3)CD62E (E-selectin)CD105 (Endoglin)CD106 (VCAM-1)CD144 (VE-cadherin)CD146CD202b (Tie2)CD309 (FLK1)PodoplaninVEGF receptor 3

Endothelial cells

25 h = Upregulated i = Downregulated � = Key marker = Subset

Other cell type markers