High Speed, Full-Spectrum Spinning-disk Confocal

Principle of Confocal MicroscopyPrinciple of Confocal Microscopy

• Multi point scanning; 1000 beams of light at any given time

• Scans 1000 times per second

• Two (or more) available pinhole aperture (40μm ,70 μm or custom)

• Advantages: Real time confocal; high speed; full spectrum; less phototoxicity

NA

MDOPTIMAL

5.0

OBJECTIVEAIRY NAr

61.0

The Nipkow DiskThe Nipkow Disk

x-y Resolution

Pin-hole diameter

Non Confocal Confocal

Mouse intestineCytox green Nucleus/ Alexa568

Nematode embryoGFP- TubulinMax. projection

Widefield vs Widefield vs ConfocalConfocal

3D Reconstruction3D Reconstruction

Confocal 3D reconstruction of GFP tagged membrane receptor in Agrobacterium tumefaciens leaves with CARV LX spinning disk system by Crest.

Courtesy of Prof. Giulia De Lorenzo, Università "La Sapienza" di Rome, Italy.

• Nikon TiS, TE 200/300/ 2000

• Olympus IX and IX 2 series

• Zeiss Axiovert 200, Axio Examiner, Axio Observer

• Leica

Convert your existing inverted microscope to a confocal imager:

Stand-alone Confocal headStand-alone Confocal head

FRAP MODULE:

Built-in FRAP mirrors and aperture for CrEST FRAP module or existing customer FRAP system

• Lasers• LEDs• Liquid lightguide coupled lamps

(Mercury, Xenon, etc)

Supported lightsouces:Supported lightsouces:

• Scans the whole sample simultaneously allowing fast image acquisition in real time → high speed ion imaging

• Application specific CCD camera can produce high resolution images and image capture speeds up-to 150 fps (10k rpm)

• CCD camera as a detector instead of a PMT (high quantum efficiency, high dynamic range)

• Low intensity high frequency scanning together with high QE CCD’s reduces photobleaching → high speed live cells imaging

• Full spectrum confocal

Advantages of the spinning disk confocalAdvantages of the spinning disk confocal

DAPI-GFP-TRITC color combine

10ms exposure-time confocal image