#931

7St

ore

at 4

°CGSK-3β (27C10) Rabbit mAb (Alexa Fluor® 488 Conjugate)

Rev. 03/17/11

n 100 µl (50 tests)

New more concentrated formulation

FEndogenous

H, M, R, Mk Rabbit IgG

Description: This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #9315 reacts with human, mouse, rat and monkey GSK-3β. CST expects that GSK-3β (27C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize GSK-3β in these species.

Background: Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulated glycogen syn-thesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphory-lates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signal-ing pathway required for Drosophila, Xenopus and mam-malian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Specificity/Sensitivity: GSK-3β (27C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total GSK-3β protein.

Source/Purification: Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human GSK-3β. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.

Background References: (1) Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274–279.

(2) Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135–141.

(3) Cross, D.A. et al. (1995) Nature 378, 785–789.

(4) Nusse, R. (1997) Cell 89, 321–323.

(5) Diehl, J.A. et al. (1998) Genes Dev. 12, 3499–3511.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.

Recommended Antibody Dilutions: Flow Cytometry 1:50

For application specific protocols please see the web page for this product at www.cellsignal.com.

Please visit www.cellsignal.com for a complete listing of recommended companion products.

Orders n 877-616-CELL (2355)orders@cellsignal.com

Support n 877-678-TECH (8324)info@cellsignal.com

Web n www.cellsignal.com

Applications Species Cross-Reactivity Isotype

Even

ts

GSK-3β (Alexa Fluor® 488)

Flow cytometric analysis of SEM cells using GSK-3β (27C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to the Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) #4340 (red).

Growth factors, insulin, etc.

PTENAkt/PKB Ser241

Thr308

Ser473

PIP3

PIP3Mem

bran

e

P

PDK1

P P P P PPP P P P

P P

P

P

Receptor

PI3KPPP

P

GSK-3α/β

Ser535/539

P

PP

P

eIF2Bε

Ser712

Ser33/37 Thr41/45

β-catenin

β-catenindegradation

P P P

Tau

P PP P

P

Ser262/356 cyclin D1

Thr286

glycogensynthase

PTSC2

cyclin D1degradation

tubulinpolymerization

glycogensynthesis

Ser8 Ser641protein

synthesis

mTORsignaling

Ser9 Ser21

LRP

Frizzled

Gαq/o

Wnt

TSC1

mTOR

SIN1GβLRictor

Ser713

P

3

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Entrez-Gene ID #2932 Swiss-Prot Acc. #P49841

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine

Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected

Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide

© 2

011

Cell

Sign

alin

g Te

chno

logy

, Inc

.Ce

ll Si

gnal

ing

Tech

nolo

gy® is

a tr

adem

ark

of C

ell S

igna

ling

Tech

nolo

gy, I

nc.