Figure S2: A2UCOE gives rise to stable eGFP expression in P19 cells compared to SFFV and EF1α.

D3 D7 D11 D14

VCN 0.32

VCN 0.41

VCN 2.1

D18

VCN 0.05

VCN 0.22

VCN 0.45

D3 D7 D15 D19 D24

D3 D7 D11 D14 D18

VCN 0.15

VCN 0.22

VCN 0.38

% E

GF

P e

xp

res

sio

n%

EG

FP

ex

pre

ss

ion

% E

GF

P e

xp

res

sio

n

A

B

C

P19 cells were transduced with different concentration of virus. eGFP expression was assessed by FACS at different time points. A: cells transduced with LV-SFFV-eGFP vector; B: cells transduced with LV-EF1α-eGFP vector; C: cells transduced with LV-UCOE-eGFP vector. VCN: vector copy number per cell determined by Q-PCR. A declined EGFP expression occurs in cells transduced with LV-SFFV-eGFP and LV-EF1α-eGFP vectors at the different concentration of viruses. However, a stable eGFP expression is remained in cells transduced with LV-UCOE eGFP vector at different concentration of virus.