FACULTY PROFILE

Name : Humaira Farooqi

Designation : Assistant Professor

Department : Biotechnology Faculty : Science E-mail : hfarooqi@jamiahamdard.ac.in Phone : 26059688 Ext: 5504 Area of specialization : Protein Biochemistry and Stress Biology.

Current Research

Stress Biology : Our current study focuses on the hepatoprotective effect of Genistein and GPLC on the oxidative liver damage induced by D-GalN. We employ animal models for our study and the study evaluates the antioxidant and hepatoprotective effect in-vivo. The oxidative damage is induced by using a potent hepato-toxin: D-GalN, known for causing damage that closely resembles viral hepatitis. So far, we have evaluated the hepatoprotective and antioxidant effect of Genistein and GPLC in which we monitored the effect of these two protective compounds at tissue as well. Oxidative stress is one of the leading health problems faced by the population worldwide. The consequences range from mild inflammatory reactions to the cellular and tissue changes that lead to the transformation of normal cells into the malignant ones. Till date there has not been any treatment or therapy that could combat efficiently with oxidative stress. Protein Folding: Protein Stability determination/Mechanism of Protein stabilization.

The targets of oxidative damage are vital biomolecules which contribute to the proper functioning state of a cell and tissue at molecular level. We are working on the conformational changes in proteins exposed to different stress conditions in vitro, evaluating its mechanism using Circular dichorism and in-silico study.

Selected Publications:

1. Sharma S1, Mayank AK, Nailwal H, Tripathi S, Patel JR, Bowzard JB, Gaur P, Donis RO, Katz JM, Cox NJ, Lal RB, Farooqi H, Sambhara S, Lal SK. Influenza. A viral nucleoprotein interacts with cytoskeleton scaffolding protein α-actinin-4 for viral replication. FEBS J. 2014 Jul;281(13): 2899-914 Impact: 3.986; ISSN: 0014-5793

2. Manu Saini, Athar Ali Khan, Madhu Bala, M Z Abdin, Humaira Farooqi. Development of a validated HPTLC method for quantification of esculin in different fractions of cichorium intybus leaf extract. Int J Pharm, Pharm Sci , 2014. Impact: 0.9; ISSN- 0975-1491.

3. Manu Saini, Madhu Bala Humaira Farooqi, Abdin M.Z, Jagdish Prasad Renoprotective activity of Hippopahe leaf extract in total body 60Co-Gamma irradiated mice: An Oxidative and Histopathology study. Int J Pharm, Pharm Sci , 2014. Impact : 1.19

4. Anand Kumar Keshri, Humaira Farooqi. Evaluation of the Effect of Hydrogen Peroxide (H2 O2 ) on Haemoglobin and the Protective Effect of Glycine. The International Journal of Science and Technoledge. 2014 Impact: 0.675; ISSN: 2321 919 X

5. Ajaz A Ganai, Amjid Ahad, Sadaf Jahan, M.Z. Abdin, Humaira Farooqi. Glycine propionyl l-carnitine attenuates D-Galactosamine induced fulminant hepatic failure in wistar rats. Chemico-biological interactions 2014. Impact : 3.154 ISSN

6. Ahmad Ganai, Amjid Ahad, Ahmad Ganaie, M Z Abdin, Humaira Farooqi. Glycine Propionyl L-Carnitine (GPLC) ameliorates D-galactoseamine (D-GALN) induced oxidative stress in rats by preventing lipid peroxidation and maintaining the redox potential. International Journal of Pharma and Bio Sciences 2013 . Impact Factor:2.958, ISSN 0975-6299.

7. H. Farooqi, A. Mahmood, D. S. Jairajpuri, F. Ahmad, S. Ali . Boron increases the transition temperature and enhances thermal stability of heme proteins. Journal of Thermal Analysis and Calorimetry. 2011. Impact Factor :2.206 ISSN: 1388-6150 (print version) ISSN: 1588-2926 (electronic version).

8. Shakir Ali, Humaira Farooqi, Ram Prasad, Mohammad Naime, Indusmita Routray, Savita Yadav, Faizan Ahmad Boron stabilizes the peroxide mediated changes in the structure of heme proteins International Journal of Biological Macromolecules 47 (2010) 109–115. Impact Factor: 3.096 ISSN:01418130.

9. Amjad Masood Husaini, Mallik Zainul Abdin, Ghulam Ahmad Parray, Gulzar Singh Sanghera, Imtiyaz Murtaza, Tanweer Alam, Dinesh Kumar Srivastava Humaira Farooqi. Vehicles and ways for efficient nuclear transformation in plants. GM crops 11/2010. Print ISSN: 2164-5698 Online ISSN: 2164-5701.

10. Humaira Farooqi; Hamid Nawaz Khan; Richa Gupta;

Anwar Habib; Parwaiz Akhtar;

Showkat Rasool Mir; Kamran J. Naquvi New Trends of Diabetes Therapy type II of the Animal Model. International Journal of Pharmaceutical and Clinical Research 2010; 2(2): 80-89 Impact Factor:1.117 ISSN: 0975 1556.

11. Hamid Nawaz Khan, Richa Gupta, Humaira Farooqi, Anwar Habib, Parwaiz Akhtar Biochemical analysis of Glycine Max Seeds under different germinating periods and densitometric analysis of Geinstein Journal of Phytology 2010, 2(6): 83-86 ISSN: 2075-6240

12. Hamid Nawaz Khan, Parwaiz Akhtar Altaf Khan, Jafar Salamat Khan and Humaira Farooqi Rice bran oil – palm olein oil blend may attenuate the risk of cardiovascular diseases: results

of an in vivo study International Research Journal of Plant Science Vol. 1(6) pp. xxx-xxx, December, 2010 Impact Factor: 1.54 ISSN: 1058-5893

13. Shakir Ali, Sonica Pawa, Mohammed Naime, Ram Prasad, Tasleem Ahmad, Humaira Farooqi and Hina Zafar Role of mammalian cytosolic molybedenum Fe-S flavin hydroxylases in hepatic injury. Lif Sci. 2008 Mar 26; 82 (13-14):780-8. Epub 2008 Jan 30. Impact Factor:2.296; ISSN: 0024-3205

RESEARCH EXPERIENCE: OVER 12 YEARS

2010- Till date- Research experience in the field of stress Biology and Protein

biochemistry. We have contributed to:

Protein Folding: Protein Stability determination/Mechanism of Protein stabilization by osmolytes/Protein folding intermediates. We are working on the hepatoprotective effect of Genistein and Glycine propionyl l-carnitine in D-Galactosamine induced liver injury.

July 2008- July 2010- Two years research experience in Plant biotechnology (Centre of transgenic plant development) Jamia Hamdard. Validation of herbal extracts for disease

prevention.

Jan 2008 – June 2008: Worked as a Research Associate (RA) in the project entitled

Role of Boron in Ameliorating Oxidative Stress and Liver Cell Necrosis: Implications

in Liver Diseases” at Department of Biochemistry, Faculty of Science, Jamia Hamdard.

August, 2004- Dec 2007: Worked as a Senior research fellow (SRF) in the project entitled “Role of Boron in Ameliorating Oxidative Stress and Liver Cell Necrosis: Implications

in Liver Diseases” at Department of Biochemistry, Faculty of Science, Jamia Hamdard, and New Delhi, sponsored by Indian Council Of Medical Research (ICMR), Govt. of India. January 2002 to 2007: Six years of research experience in the Protein Biochemistry field, in thesis work entitled “Effect of Boron on Structure of Proteins under Oxidative Stress

and Heat Stress” at Department of Biochemistry, Faculty of Science, Jamia Hamdard, and New Delhi.

Research Supervision

Abstracts/ papers in Conferences (Participated/Presented):

1. National Seminar on Plant Biotechnology – challenges and opportunities in 21st

Centaury March 3-4 , 2014 Department of Botechnology Faculty of Science Jamia

Hamdard .

2. National Conference on Chronic Inflammatory Disorders , Department of

Biochemisrty , Faculty of Science and Departmnet of Medica Biochemistry HIMSR and

HAHC Hospital Jamia Hamdard 13-5 Feb. 2014.

3. Training programme on Transgenic technology Methods and Applications March

12-17, 2012 Centre for Transgenic Plant development Department of Biotechnology and

Faculty of Engineering and Interdisciplinary Sciences Jamia Hamdard.

4. Natural Seminar on current Trends in Secondary Plant Metabolites Research March

19-20 2012 Department of Biotechnology Faculty of Science Jamia Hamdard.

5. National Seminar on Proteomics Based Biomarkers for Animal and Plant Diseases

March 07-01-2012 Department of Biotechnology Jamia Hamdard.(Oral Presentation).

6. International Interdisciplinary Science Conference (I-ISC, 2012) on Protein Folding

and Diseases. Dec. 8-10 2012 Jamia Millia Islamia.

7. III Seminar cum Hands-on Training on Computational Protein Structure Prediction

Department of Biochemistry Jamia Hamdard 2-29 Nov. 2011

Ph.D. PG

Supervised (as Supervisor)

-

30

Supervized (as Co-supervisor)

2

Enrolled (as supervisor) 2

8. Training programme on transformation and molecular characterization of medicinal

plants and animals Dec, 16-21, 2010. Centre for transgenic plant development

department of biotechnology Jamia Hamdard New Delhi.

9. National workshop on Biotechnological approach for conservation of Unani Herbs

and enhancing their Medicinal efficacies 8-11 Nov 2010

10. Centre for Transgenic Plant Development, Department of Biotechnology 30th Nov -

5th Dec 2009 Jamia Hamdard. 11. Participated in Training Programme on Tissue Culture, Transgenic Technology and

Biotechnology at Jamia Hamdard,New Delhi from 30 Nov to 5th Dec 2009 12. Participated in Interdisciplinary Science Conference -2007 on Recent Trends in

Research in Biological Sciences held on December 7, 2007 at Jamia Millia Islamia, New-Delhi. 13. Seminar on “Workshop on NMR Spectroscopy and its Application”, organized jointly by Department of Chemistry, Jamia Hamdard and Indian Society of Analytical Scientists-Delhi Chapter, held on 18-19 August 2006 at Jamia Hamdard, New Delhi India. 14. Presented a poster at American Society of Cell Biology, held at San-Francisco, California. 2005, USA. 15. Seminar on “Applications of Modern Analytical Techniques in Chemical and Biological

Sciences”, organized jointly by Department of Chemistry, Jamia Hamdard and Indian Society of Analytical Scientists-Delhi Chapter, held on 9th February 2005 at Jamia Hamdard, New Delhi India. 16. Poster presentation at International conference on Chemistry Biology Interface

(CBISNF), conducted by Dr. B. R. Ambedakar Research Institute, New Delhi-110007 held on November 21-26, 2004 India. 17. Attended the XXVI Annual Conference of Indian Botanical Society and National

Symposium. “Plant Biology and Biodiversity in changing environment” held on Dec 29-2003 at Jamia Hamdard, New Delhi.

Awards and Honors

GRANTS

Indigenous (Jamia Hamdard): Seed Grant Project completed.

Submitted: Role of natural compounds in folding of metal catalyzed proteins: Structural and molecular studies. (Submitted to UGC) To develop microbial species with high yield production of the enzyme and process required for low density polyethylene [LDPE] degradation.(Submitted to DST- Woman Scientist Scheme A)

Fellowships

ICMR Fellowship from Aug 2005 – June -2008

HNF (Hamdard National Foundation Fellowship from 2002 -2004)

Participation in corporate Life:

Coordinator Placement cell for Msc. Biotechnology students Jamia Hamdard .We are

doing the campus placements of students in collaboration with industries and other

corporate sector.

Extra Curricular Activities

Convener of Seminars/ Conferences.

Member Cultural Committee Jamia Hamdard.

Member Academic Council Jamia Hamdard.

Warden Girls hostels (2010-2012) Jamia Hamdard.