Curriculum vitae: Dimitrios Anagnostopoulos

Chemist, M.Sc., Ph.D. Biochemistry

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CURRICULUM VITAE

Dr. DIMITRIOS ANAGNOSTOPOULOS

Surname: Anagnostopoulos (Αναγνωστόπουλος) First name: Dimitrios (Δημήτριος) Date of bearth: 1982, 9th of March Address: Nikolaou Plastira 42, 17121 Nea Smirni, Attikis Phone (mobile): +306951417950 E-mail: d.anagnostopoulos@inn.demokritos.gr, danagnostopoulos@gmail.com,

Website: http://inn.demokritos.gr/project/free-radicals-in-bio-and-nano-technology/

Education

■ 1999-2003: Undergraduate degree in Chemistry, Department of Chemistry, University of Athens (Awarded on October

2003)

■ 2003-2005: MSc degree in Biochemistry, Department of Chemistry, University of Athens, on the «Synthesis and

characterization of trans polyunsaturated fatty acids and their derivatives. Study of their biological role» (Awarded on October

2005)

■ 2005 - 2010: PhD degree in Biochemistry, Department of Chemistry, University of Athens. Thesis title «Fatty Acid Amide

Hydrolase in Tetrahymena: Kinetic studies, endogenous substrates and characterization of the corresponding gene» (Awarded

on May 2010)

Research experience

■ August 2014- Today: Post Doctoral Researcher at the Institute of Nanoscience and Nanotechnology, (INN) N.C.S.R.

“Demokritos”

■ May 2012 – 2014: Post Doctoral Researcher at The Goulandris Natural Museum for the Research Programm : Development

and Screening of Novel beta Αmyloid Peptide Inhibitors for Alzheimer’s Disease

■ 2007 June - December: Visiting Researcher at Northeastern University, Center for Drug Discovery (Boston, Massachusetts)

■ 2004 February, 2005 October- December, 2014 June: Visiting Researcher at ISOF CNR (Βologna, Italy)

Research programs participations

■ ΥΔΙΣΕ Advanced materials and devices for collection and energy management. Ε.Ε. 1812

■ 09ΣΥΝ-21-1003 (TreatAD): Development and Screening of Novel beta Αmyloid Peptide Inhibitors for Alzheimer’s Disease

with scientific supervisor Prof. A. Tsarbopoulos

■ COST Action BM1201: Biomimetic Radical Chemistry

■ COST Action BM1104: Mass Spectrometry Imaging: New Tools for Healthcare Research

■ ΠΕΝΕΔ – 2003 (Greek research program): with scientific supervisor Α. Siafaka Kapadai

■ COST Action MB 0603: Free radicals in chemical biology

■ Sulfur radical chemistry of biological significance, the protective and damaging roles of thiol and thioether radicals, as young

researcher with scientific supervisors Chryssostomos Chatgilialoglu and Carla Ferreri

Curriculum vitae: Dimitrios Anagnostopoulos

Chemist, M.Sc., Ph.D. Biochemistry

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Memberships in Research Organizations

■ ASMS, American Society for Mass Spectrometry

■ HMSS, Hellenic Mass Spectrometry Society

■ Greek Society of Biochemistry and Molecular Biology

■ Greek Lipid Forum, member of Euro Fed Lipid

■ The International Cannabinoid Research Society

■ Greek Chemistry Society

Conference Organizer

■ 8th FEBS Young Scientist Forum - YSF2008 "Cell Harmony" June 26-28, 2008. Loutraki, Greece (http://www.febs-iubmb-

2008.org/young.asp)

■ «Conference of postgraduate students of Chemistry department». March 27-28, 2009. Department of Chemistry,

University of Athens (http://epikouros.chemicalworld.gr/)

Publications

1. E. Gikas, F.N. Bazoti, M. Katsimardou M, D. Anagnostopoulos, K. Papanikolaou, I. Inglezos, A. Skoutelis, G.L. Daikos, A.

Tsarbopoulos. “Determination of colistin A and colistin B in human plasma by UPLC-ESI high resolution tandem MS:

Application to a pharmacokinetic study.” J Pharm Biomed Anal. 83:228-236 (2013). (link to pubmed)

2. A.G. Xanthopoulou, D. Anagnostopoulos, K. Vougas, A.K.. Anagnostopoulos, A.G. Alexandridou, G. Spyrou, A. Siafaka-

Kapadai and G.Tsangaris. “A two dimentional proteomic profile of Tetrahymena thermophila whole cell lysate.” In Vivo.

24:443-56. (2010) (link to pubmed)

3. D. Anagnostopoulos, C. Rakiec, J. Wood, L. Pandarinathan , N. Zvonok, A. Makriyannis , A. Siafaka-Kapadai. “Identification

of endocannabinoids and related N-acylethanolamines in tetrahymena. A new class of compounds for Tetrahymena.” Protist.

161:452-65 (2010) (link to pubmed)

4. A. Evagorou, D. Anagnostopoulos, E. Farmaki and A. Siafaka-Kapadai. “Hydrolysis of 2-arachidonoylglycerol in Tetrahymena

thermophila. Identification and partial characterization of a Monoacylglycerol Lipase-like enzyme.” Eur J Protistol. 46:289-

97 (2010) (link to pubmed)

5. E. Gkini, D. Anagnostopoulos, M. Mavri-Vavayianni, A. Siafaka-Kapadai. “Metabolism of 2-acylglycerol in rabbit and human

platelets. Involvement of monoacylglycerol lipase and fatty acid amide hydrolase.” Platelets 20:376-85 (2009) (link to pubmed)

6. C. Ferreri, D. Anagnostopoulos, I.N. Lykakis, C. Chatgilialoglu, A. Siafaka-Kapadai. “Synthesis of all-trans anandamide: a

substrate for fatty acid amide hydrolase with dual effects on rabbit platelet activation.” Bioorg. Med. Chem. 16:8359-65 (2008)

(link to pubmed)

7. D. Anagnostopoulos, C. Chatgilialoglu, C. Ferreri, A. Samadi and A. Siafaka-Kapadai. “Synthesis of all-trans arachidonic

acid and its effect on rabbit platelet aggregation.” Bioorg. Med. Chem. Lett. 15:2766-70 (2005) (link to pubmed)

8. V. Karava, M.P. Zafiriou, L. Fasia, D. Anagnostopoulos, E. Boutou, C.E. Vorgias, M. Maccarrone and A. Siafaka-Kapadai.

“[3H] Anandamide metabolism by Tetrahymena pyriformis in vitro. Anandamide metabolism by Tetrahymena pyriformis in

vitro. Characterization and identification of a 66kDa FAAH.” Biochimie. 87:967-974 (2005) (link to pubmed)

Curriculum vitae: Dimitrios Anagnostopoulos

Chemist, M.Sc., Ph.D. Biochemistry

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Conference Publications

1. A. Georgakopoulos, D. Anagnostopoulos, I.K. Kostakis, E. Gikas, B. Mavroidi, A. Tsarbopoulos, M. Pelecanou,

A.L. Skaltsounis. “Design, synthesis and biological evaluation of novel hydroxytyrosol derivatives as potential

anti-amyloidogenic agents” EFMC-ISMC 2014 XXIII International Symposium on Medicinal Chemistry Lisbon,

September 7-11, Portugal, (2014) (poster presentation)

2. A. Georgakopoulos, D. Anagnostopoulos, I.K. Kostakis, E. Gikas, B. Mavroidi, A. Tsarbopoulos, M. Pelecanou,

A.L. Skaltsounis. “Synthesis of novel hydroxytyrosol analogues as potential anti- amyloidogenic agents” 62th

International Congress and Annual Meeting of the Society for Medical Plant and Natural Product Research. 31

August-4 September, Guimares, Portugal (2015) (poster presentation)

3. N.S. Koulakiotis, D. Anagnostopoulos, I. Kostakis, I. Chalatsa, D. Sanoudou, A, Tsarbopoulos. “Natural Products:

A Promising Source for Potential β-Amyloid Inhibitors.” 62st ASMS Conference on Mass Spectrometry and

Allied Topics. June 15 - 19. Baltimore, MD (2014) (poster presentation) z

4. D. Anagnostopoulos, E. Gikas, K. Papanikolaou, P. Haritidis, A. Skoutelis, G. Daikos, A. Tsarbopoulos

“Determination of Colistin pharmacokinetics in human plasma and bronchoalveolar lavage by anovel UPLC-ESI

QTOF MS/MS methodology.” 61st ASMS Conference on Mass Spectrometry and Allied Topics. June 9 - 13.

Minneapolis, MN. (2013) (oral presentation)

5. Π. Μερμίγκη, Δ. Αναγνωστόπουλος, Ν.Σ. Κουλακιώτης, Α.Β. Φερλέμη, Π. Κορδοπάτης, Α. Τσαρμπόπουλος, Φ.

Λάμαρη. “Χρωματογραφικη ανάλυση των φαινολικών συστατικών των φύλλων μυρτίλλων vaccinium

corymbosum.” 16ο Πανελλήνιο Φαρμακευτικό Συνέδριο. Σελ.34 (pp03018) 31 Μαΐου-3 Ιουνίο, Τεχνόπολις-

Γκάζι. (2013) (poster presentation)

6. D. Anagnostopoulos, C. Freni, J. Wood, L. Pandarinathan, A. Makriyannis, and A.Siafaka-Kapadai. “Evidence

for the presence of N-acylethanolamines and acylglycerols in Tetrahymena.” FEBS J. 275 (Suppl1), 439 (2008)

(poster presentation)

7. Α. Evagorou, D. Anagnostopoulos, E. Farmaki, A. Siafaka-Kapadai “2-AG degradation in Tetrahymena.

Charachterization of a monoakylglycerol lipase-like enzyme.” FEBS J. 275 (Suppl1), 399 (2008) (poster

presentation)

8. E. Gkini, D. Anagnostopoulos, A. Siafaka-Kapadai. “Inactivation of the endocannabinoid 2-arachidonylglycerol

in rabbit platelets. Identification and characterization of monoacylglycerol lipase.” FEBS J. 275 (Suppl1), 351

(2008) (poster presentation)

9. D. Anagnostopoulos, E. Boutou, C.E. Vorgias , and A. Siafaka-Kapadai. “Cloning of FAAH like genes in

Tetrahymena species. 17th Annual symposium on the cannabinoids.” 26 June-1 July St-Sauveur, Canada Abs. p.

105 (2007) (poster presentation)

10. D. Anagnostopoulos, I. Lykakis. C. Ferreri, C. Chatgilialoglu and A. Siafaka- kapadai. “All trans anandamide

synthesis and its effects on platelet functions. Interference with cis-anandamide hydrolysis by FAAH. 16th Annual

symposium on the cannabinoids.” Abs. p. 117, June 24-28 Tihany, Hungary (2006) (poster presentation)

11. Ε. Farmaki, D. Anagnostopoulos and A. Siafaka-Kapadai. “The endocannabinoid system in rabbit brain. Evidence

for the presence of CB2 receptors. 16th Annual symposium on the cannabinoids.” Abs. p. 99, June 24-28, Tihany,

Hungary (2006) (poster presentation)

12. A. Siafaka-Kapadai, E. Farmaki, A. Theodoridou and D. Anagnostopoulos. “MAGL (monoacylglycerolipase)

activity it Tetrahymena. 16th Annual symposium on the cannabinoids.” Abs. p. 90, June 24-28 Tihany, Hungary

(2006) (poster presentation)

Curriculum vitae: Dimitrios Anagnostopoulos

Chemist, M.Sc., Ph.D. Biochemistry

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13. E. Farmaki, P. M. Zafiriou, D. Anagnostopoulos, M. Maccarrone, A. Siafaka-Kapadai. “NAPE-PLD-like and

cannabinoid receptors in Tetrahymena pyriformis.” Newsletter 52, 70 57th Meeting, Hellenic Society of

Biochemistry and Molecular Biology, Athens, Greece (2005) (poster presentation)

14. E. Farmaki, P. M. Zafiriou, D. Anagnostopoulos, M. Maccarrone, A. Siafaka-Kapadai. “The endocannabinoid

system in the protozoon Tetrahymena pyriformis.” 1st Greek Lipid Forum. June 8-9, Athens, Greece (2005) (poster

presentation)

15. D. Anagnostopoulos, C. Chatgilialoglu, C. Ferreri, A. Samadi and A. Siafaka-Kapadai. “Synthesis of all-trans

PUFAs and congeners. Study of their biological activity.” 1st Greek Lipid Forum. June 8-9, Athens, Greece (2005)

(poster presentation)

16. A. Siafaka-Kapadai, D. Anagnostopoulos, P.M. Zafiriou, L. Fasia. “Anandamide metabolism and its effect on

human and rabbit platelet avtivation. A comparative study. 2nd international conference: phospholipases A2 & 8th

international congress: platelet-activating factor and related lipid mediators.” Abs. p. 119, October 6-9 Berlin

(2004) (poster presentation)

17. D. Anagnostopoulos, C. Chatgilialoglu, C. Ferreri, A. Samadi and A. Siafaka-Kapadai. “All-trans PUFA synthesis

by the thiyl radical-catalysed methodology and their effects on rabbit platelet aggregation.” Chem. Phys. Lipids,

130: 67 (2004) (poster presentation)

18. D. Anagnostopoulos, T. Hadjiloe, M.P. Zafiriou, L. Fasia and A. Siafaka-Kapadai, “Semi-synthesis and biological

activity of N-acyl-phosphatidyl-ethanolamines.” Chem. Phys. Lipids, 130: 68 (2004) (poster presentation)

Participation in research networks meetings - workshops

1. D. Anagnostopoulos, E. Gikas, K. Papanikolaou, A. Skoutelis, G. Daikos, A. Tsarbopoulos “Colistin

Pharmacokinetics in Human Plasma by a Novel UPLC-ESI QTOF MS/MS methodology.” Middle Eastern and

Mediterranean Sea Region Countries Mass Spectrometry – MASSA 2013 Workshop. P.17 (p.02) 30 June –4 July

4, SIENA (Italy) (2013) (poster presentation)

2. D. Anagnostopoulos, Α. Evagorou, E. Gkini, A. Makriyannis, A. Siafaka-Kapadai. “The endocannabinoid system

is present in the unicellular organism Tetrahymena. Identification of N-acylethanolamines.” 1st annual satellite

symposium on the cannabinoids therapeutic potential of the cannabinoids: Present and future", Limassol, Cyprus,

(2008) (oral presentation)

3. D. Anagnostopoulos, I. Lykakis. C. Ferreri, C. Chatgilialoglu and A. Siafaka-Kapadai. “Evaluating geometrical

trans lipid isomers for an antisense strategy: Synthesis of all trans arachidonic acid and all-trans anandamide, and

their activity on rabbit platelet aggregation.” SULF.RAD. Network Meeting, February 1-5, Paris, France, (2006)

(oral presentation)