Anteo Diagnostics, Ltd. Unit 4, 26 Brandl Street, Brisbane Technology Park, Eight Mile Plains, QLD 4113, Australia

Coupling Kit 1 µm Magnetic Particles

The AMG Difference Greater sensitivity, increased reproducibility and decreased development times are just some of the benefits Mix&Go™ provides – all in a ready-to-use, pre-activated particle format and delivered in the new AMG (Anteo Mix&Go) Coupling Kit. The kit includes everything you need to enable fast and easy binding of proteins to 1 µm magnetic particles in a one-step reaction.

The kit is suitable for coupling a broad range of biomolecules including; peptides, antigens and antibodies. Coupled particles are compatible with a range of fluorescent and chemiluminescent assays.

Mix&Go Coupling Efficiency

Mix&Go coupling efficiency is comparable to standard covalent coupling without the handling limitations users experience with covalent coupling such as chemical instability and multiple steps impacting on particle dispersity.

Fast, Easy and Hassle Free Conjugation In one-step you can bind your protein of interest to the pre-activated 1 µm magnetic particles using the AMG Coupling Kit (as illustrated below). It’s a fast, easy and user-friendly procedure that will let you get on with the research that matters.

Now Available One-Step Protein Coupling

Weigh out, add EDC

30 min

room temp

Bare COOH

particles

Antibody

2-16 hr

incubation

Wash

Storage Buffer

Amide antibody coupled particles

Bare TOSYL particles

Antibody

16-24 hr

incubation at 37oC

Wash

Storage Buffer

Tosyl antibody coupled particles

Antibody

1 hr room

temp

Mix&Go™ activated particles

Wash

Storage Buffer

Mix&Go™ antibody coupled particles

Mix&Go™ total coupling time < 2 hr

Tosyl total coupling time > 16 hr

Amide total coupling

time: up to 16 hr

Coupling Kit Fast & Easy

One-Step Coupling

V1.0 07/14

Coupling Kit Performance: Binding Capacity: > 20 µg IgG/mg Monodispersity: > 90%

Kit components:

•  Activated 1 µm Magnetic Particles •  Coupling Buffer •  Blocking Buffer •  Storage Buffer •  Microcentrifuge

tubes

Anteo Cat #: A-SMPCKMP-10 (1 mL; 10 mg/mL) A-SMPCKMP-30 (3 mL; 10 mg/mL)

V1.0 07/14

No Particle Activation Required AMG Coupling Kits employ Anteo’s novel Mix&Go proprietary surface coating.

The elimination of an activation step and pre-treatment of proteins allows users to design and generate novel and complex particle conjugates by incorporating more than one protein in co-coupling reactions.

Exceptional Stability Activated particles can withstand the following conditions:

•  Temperature: 1 hr at 60°C •  Harsh chemical conditions: 1% Tween-20,

50% DMSO, 4M Urea. Protein coupled particles are stable under: •  pH extremes: pH 2- 10 •  Temperature: 1 hr at 60°C •  Harsh Chemical treatments: ≤5% Tween20, ≤0.05M EDTA, ≤1M NaCl, ≤8M Urea, ≤50% DMSO

The Science Behind Mix&Go

The surface chemistry incorporated into this product provides a novel approach for attaching fragile proteins to synthetic surfaces that is distinct to the traditional methods of passive adsorption and covalent binding. An aqueous cationic polymer, Mix&Go, forms functional protein mono-layers that are highly stable.

Anteo Diagnostics has a suite of application specific reagents specifically formulated for improved performance on a range of synthetic surfaces. The AMG product line features products incorporating the Mix&Go chemistry.

Contact To place an order or to learn more;

•  email sales@anteodx.com •  visit www.anteodx.com

Experience the Mix&Go™ Advantage Today Product Highlights:

•  Simplified Handling •  Excellent Binding Capacity •  High Reproducibility •  High Superparamagnetic

Functionality •  Highly Monodispersed •  Excellent Colloidal Stability

Compatibility of AMG activated particles during coupling. AMG activated particles were either stress tested for 1 hr or coupled in the presence of the indicated conditions. Performance was assessed by binding to mouse IgG and assayed for loading capacity with goat anti-mouse IgG-RPE.

0 5,000

10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 50,000

MFI

Mix&Go Condition and Chemical Compatibility during Coupling

Loading Assay: Mouse IgG - GAM-RPE

Coupling Kit 1 µm Magnetic Particles

Anteo Diagnostics, Ltd. Unit 4, 26 Brandl Street, Brisbane Technology Park, Eight Mile Plains, QLD 4113, Australia

Anteo Cat #: A-SMPCKMP-10 (1 mL; 10 mg/mL) A-SMPCKMP-30 (3 mL; 10 mg/mL)

0

10,000

20,000

30,000

40,000

50,000

60,000

MFI

Plate Fluorescence Loading Assay x-RPE (as indicated)

The AMG Coupling Kit has broad applicability and can be used to couple a range of proteins of different size. Actual loading will vary depending on the protein coupled, the size of the protein and the quantity of protein added to the coupling reaction. Abbreviations; mouse (mo), goat anti-mouse (GAM), rabbit (rb), goat anti-rabbit (GAR), human (hu) and goat anti-human (GAH).