Analysis of Glycoproteins on Proteomix® SAX-NP10

PP1009

Analysis of Transferrin Holo (untreated and treated with Neuraminidase) on Proteomix® SAX-NP10

Column: Proteomix® SAX-NP10, 4.6 x 250 mm Flow rate: 1 mL/min Detection: UV 280 nm Sample: 50 μg of each untreated and

neuraminidase treated transferrin Holo Mobile phase A: 150 mM Tris-HCl, pH 9.0

B: A + 0.5 M NaCl Gradient: 0-20% B in 40 minutes

Analysis of Transferrin Apo (untreated and treated with Neuraminidase) on Proteomix® SAX-NP10

Column: Proteomix® SAX-NP10, 4.6 x 250 mm Flow rate: 1 mL/min Detection: UV 280 nm Sample: 50 μg of each untreated and

neuraminidase treated transferrin Apo Mobile phase A: 150 mM Tris-HCl, pH 9.0

B: A + 0.5 M NaCl Gradient: 0-20% B in 40 minutes

Sepax offers a variety of solutions for the analysis of Glycosylated proteins. Sepax columns permit the analytical scale separation of glycoforms on IEX. Sepax’s Proteomix SAX allows for high loading of Glycosylated proteins while still exhibiting good resolution.

For a complete listing of these and other Sepax Technologies products, go to www.sigmaaldrich.com/sepax

SDS Page Gel and Overlay of Transferrin Holo and Apo (treated and untreated) on Proteomix® SAX-NP10

Column: Proteomix® SAX-NP10, 4.6 x 250 mm, Flow rate: 1 mL/min, Detection: UV 280 nm, Sample: 50 μg of each untreated and neuraminidase treated transferrin Apo and transferrin Holo, Mobile phase A: 150 mM Tris-HCl, pH 9.0, B: A + 0.5 M NaCl, Gradient: 0-20% B in 40 minutes. On the SDS page gel, Pool 1 is the protein marker, Pool 2 is Transferrin Holo, Pool 3 is treated Transferrin Holo, Pool 4 is Transferrin Apo and Pool 5 is treated Transferrin Apo.

Transferrin Holo loading test on Proteomix® SAX-NP10

Column: Proteomix® SAX-NP10, 4.6 x 250 mm Flow rate: 1 mL/min Detection: UV 280 nm Sample: 50 μg Transferrin Holo (10 mg/mL) 100 μg Transferrin Holo (10 mg/mL) 150 μg Transferrin Holo (10 mg/mL) Mobile phase A: 150 mM Tris-HCl, pH 9.0 B: A + 0.5 M NaCl Gradient: 0-20% B in 40 minutes

For a complete listing of these and other Sepax Technologies products, go to www.sigmaaldrich.com/sepax