13567_2014_81_MOESM1_ESM.docx - Springer …10.1186... · Web viewPlasmid pCX340 carrying sopD...

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Plasmids Characteristics Reference pKD3 Carries an FRT-Cm-FRT cassette (Cm R ) [24] pKD4 Carries an FRT-Km-FRT cassette (Km R ) pKD46 Carries the λ-Red genes under control of P araB ; temperature sensitive replication (Cb R ) pCX340 Plasmid pCX340 (Tc R ) carrying β-lactamase gene [27] pCX340sopD (pICC612) Plasmid pCX340 carrying sopD β-lactamase fusion (Tc R ) pFPVmCherry Plasmid carrying mCherry ORF (Cb R ) (Addgene) [28] pEGFP-actin Plasmid encoding EGFP-actin [49] pACYC177 Plasmid pACYC177 (Km R Cb R ) [50] pACYC177sipA Plasmid pACYC177 carrying sipA from SE LA5 (Cb R ) This study pACYC177sop A Plasmid pACYC177 carrying sopA from SE LA5 (Cb R ) This study pBR322 Plasmid pBR322 (Tc R Cb R ) [51] pBR322sopE Plasmid pBR322 carrying sopE from SE LA5 (Tc R ) This study Primers Sequence Reference hilA-F 5’-GGTTTAATCGTCCGGTCGTAGTG-3’ [30] hilA-R 5’-CCTGATCCTGCATCTGAAAAGG-3’ invF4-RT 5’-GCGCCATCGATAAATGCCAGT-3’ invF1 5’-GGTGCTGACAACTATGCTAAATACGCAGG-3’ This study sipA2 5’-AGGGAACGGTGTGGAGGTAT-3’ sipA7 5’-CCAACGCAATGGCGAGTCAC-3’ [30] tufA-F 5’-TGTTCCGCAAACTGCTGGACG-3’ tufA-R 5’-ATGGTGCCCGGCTTAGCCAGTA-3’ invA-P1 5’-TTATATTGTTTTTATAACATTCACTGACTTGCTATCT GCTATCTCACCGAGTGTAGGCTGGAGCTGCTTC-3’ This study invA-P2 5’-GTGCTGCTTTCTCTACTTAACAGTGCTCGTTTACGA CCTGAATTACTGATCATATGAATATCCTCCTTAG-3’

Transcript of 13567_2014_81_MOESM1_ESM.docx - Springer …10.1186... · Web viewPlasmid pCX340 carrying sopD...

Page 1: 13567_2014_81_MOESM1_ESM.docx - Springer …10.1186... · Web viewPlasmid pCX340 carrying sopD β-lactamase fusion (TcR) pFPVmCherry Plasmid carrying mCherry ORF (CbR) (Addgene) [28]

Plasmids Characteristics ReferencepKD3 Carries an FRT-Cm-FRT cassette (CmR)

[24]pKD4 Carries an FRT-Km-FRT cassette (KmR)

pKD46 Carries the λ-Red genes under control of ParaB; temperature sensitive replication (CbR)

pCX340 Plasmid pCX340 (TcR) carrying β-lactamase gene[27]pCX340sopD

(pICC612) Plasmid pCX340 carrying sopD β-lactamase fusion (TcR)

pFPVmCherry Plasmid carrying mCherry ORF (CbR) (Addgene) [28]pEGFP-actin Plasmid encoding EGFP-actin [49]pACYC177 Plasmid pACYC177 (KmR CbR) [50]pACYC177sipA Plasmid pACYC177 carrying sipA from SE LA5 (CbR) This studypACYC177sopA Plasmid pACYC177 carrying sopA from SE LA5 (CbR) This studypBR322 Plasmid pBR322 (TcR CbR) [51]pBR322sopE Plasmid pBR322 carrying sopE from SE LA5 (TcR) This studyPrimers Sequence ReferencehilA-F 5’-GGTTTAATCGTCCGGTCGTAGTG-3’

[30]hilA-R 5’-CCTGATCCTGCATCTGAAAAGG-3’invF4-RT 5’-GCGCCATCGATAAATGCCAGT-3’invF1 5’-GGTGCTGACAACTATGCTAAATACGCAGG-3’ This studysipA2 5’-AGGGAACGGTGTGGAGGTAT-3’sipA7 5’-CCAACGCAATGGCGAGTCAC-3’

[30]tufA-F 5’-TGTTCCGCAAACTGCTGGACG-3’tufA-R 5’-ATGGTGCCCGGCTTAGCCAGTA-3’

invA-P1 5’-TTATATTGTTTTTATAACATTCACTGACTTGCTATCTGCTATCTCACCGAGTGTAGGCTGGAGCTGCTTC-3’ This study

invA-P2 5’-GTGCTGCTTTCTCTACTTAACAGTGCTCGTTTACGACCTGAATTACTGATCATATGAATATCCTCCTTAG-3’

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