32

∫ ∑§—¥¬àÕ

„π°“√∑«π Õ∫§«“¡„™â‰¥â¢Õß«‘∏’∑¥ Õ∫À“°√¥

‡∫π‚´Õ‘°·≈–°√¥´Õ√å∫‘°µ“¡«‘∏’ Compendium of

Methods for Food Analysis (2003) „πµ—«Õ¬à“ß

‡§√◊ËÕߥ◊Ë¡ º≈°“√∑¥ Õ∫π”¡“À“§à“§ÿ≥≈—°…≥–‡©æ“–

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“¡“√∂µ√«®À“ª√‘¡“≥‰¥â §«“¡·¡à𠧫“¡‡∑’ˬ߷≈–

% Recovery æ∫«à“ ¢’¥®”°—¥„π°“√µ√«®æ∫°√¥

‡∫π‚´Õ‘° (LOD) ‡∑à“°—∫ 1.40 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡

·≈–¢’¥®”°—¥∑’Ë “¡“√∂µ√«®À“ª√‘¡“≥‰¥â¢Õß°√¥

‡∫π‚´Õ‘° (LOQ) ‡∑à“°—∫ 4.64 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡

·≈–¢’¥®”°—¥„π°“√µ√«®æ∫°√¥´Õ√å∫‘° (LOD) ‡∑à“°—∫

1.65 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡ ·≈–¢’¥®”°—¥∑’Ë “¡“√∂µ√«®À“

ª√‘¡“≥‰¥â¢Õß°√¥´Õ√å∫‘° (LOQ) ‡∑à“°—∫ 5.52 ¡‘≈≈‘°√—¡

µàÕ°‘‚≈°√—¡ §«“¡∂Ÿ°µâÕß·≈–·¡à𬔄π°“√∑¥ Õ∫

µ—«Õ¬à“ß∑’Ë¡’°“√‡µ‘¡ “√≈–≈“¬¡“µ√∞“π‡∫π‚´Õ‘°·≈–

°√¥´Õ√å∫‘° ‰¥â§à“√âÕ¬≈–°“√‰¥â°≈—∫§◊π (% Recovery)

‡∑à“°—∫ 103.52 ·≈– 98.29 µ“¡≈”¥—∫ ´÷ËßÕ¬Ÿà„π™à«ß

80-110% ·≈– % RSD / RSDr πâÕ¬°«à“ 2 ¥—ßπ—Èπ

∑’˧«“¡‡¢â¡¢âπ√–¥—∫π’È¡’§«“¡·¡àπ ·≈–§«“¡‡∑’ˬߥ’Õ¬Ÿà

„π‡°≥±å∑’ˬա√—∫‰¥â ®“°º≈°“√∑¥≈Õßπ’Èæ∫«à“«‘∏’°“√

∑¥ Õ∫π’ȇªìπ«‘∏’°“√∑’ˇÀ¡“– ¡µàÕ°“√π”¡“∑¥ Õ∫

µ—«Õ¬à“ߪ√–‡¿∑‡§√◊ËÕߥ◊Ë¡∑’Ë¡’∑—Èß°√¥‡∫π‚´Õ‘°·≈–

°√¥´Õ√å∫‘°º ¡Õ¬Ÿà∑—Èß Õß™π‘¥·≈– “¡“√∂«‘‡§√“–ÀåÀ“

„π™à«ßª√‘¡“≥µË”‰¥â¥â«¬

A bstract

In this method verification of benzoic

acid and sorbic acid analysis followed by the

Compendium of Methods for Food Analysis

(2003), the characteristics used in this study were

limit of detection (LOD), limit of quantitation

(LOQ), precision and accuracy. The results showed

that the LOD and LOQ of benzoic acid in

beverage were 1.40 mg/kg and 4.64 mg/kg,

respectively whereas the LOD and LOQ of sorbic

acid in beverage were 1.65 mg/kg and 5.52 mg/kg,

respectively. The accuracy and precision were

performed by spiking the known standard solution

of benzoic acid and sorbic acid in the beverage

sample blank. The percentage of the recovery

of benzoic acid and sorbic acid were 103.52

and 98.29, respectively which were within the

acceptance range of 80-110%. Besides, the

percentage of RSD/RSDr was less than 2. These

results showed that this method performed good

accuracy and precision. Thus, this verification

study demonstrated that this method was suitable

for the determination of benzoic acid and sorbic

acid in beverages at the low concentration.

33

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34

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ºŸâº≈‘µ∑’Ë®–π”‡¢â“À√◊Õ àßÕÕ°º≈‘µ¿—≥±åÕ“À“√

®–µâÕß¡’°“√µ√«® Õ∫§ÿ≥¿“æÕ“À“√µ“¡ æ√∫.Õ“À“√

À√◊Õµ“¡¡“µ√∞“π·µà≈–ª√–‡∑»§Ÿà§â“ ®÷ß¡’§«“¡®”‡ªìπ

∑’Ë®–µâÕß∑¥ Õ∫º≈‘µ¿—≥±åÕ“À“√π—Èπ Ê ‡æ◊ËÕ„À≥â§ÿ≥¿“æ

‡ªìπ‰ªµ“¡‡°≥±å·≈–ª≈Õ¥¿—¬µàÕºŸâ∫√‘‚¿§ «‘∏’∑’Ë„™â

∑¥ Õ∫À“«—µ∂ÿ°—π‡ ’¬„πÕ“À“√¢÷Èπ°—∫™π‘¥¢Õߺ≈‘µ¿—≥±å

35

«‘∏’°“√∑¥≈Õß

°“√µ√«® Õ∫§«“¡„™â‰¥â¢Õß«‘∏’π’È„™âµ—«Õ¬à“߇ªìπ

‡§√◊ËÕߥ◊Ë¡πȔ՗¥≈¡™π‘¥∑’ˉ¡à¡’«—µ∂ÿ°—π‡ ’¬ ∑—Èß°√¥‡∫π‚´Õ‘°

·≈–°√¥´Õ√å∫‘° ·≈–∑”°“√∑¥ Õ∫‚¥¬°“√‡µ‘¡ “√≈–≈“¬

¡“µ√∞“π≈߉ª·≈–∑¥ Õ∫µ“¡«‘∏’ Compendium of

Methods for Food Analysis. 1st ed. 2003. ·≈–

µ√«® Õ∫‡æ◊ËÕ· ¥ß«à“«‘∏’°“√«—¥¡’§ÿ≥≈—°…≥–‡À¡“– ¡

°—∫«—µ∂ÿª√– ߧ尓√„™âß“π §ÿ≥≈—°…≥–‡©æ“–∑’Ë· ¥ß

§ÿ≥ ¡∫—µ‘¢Õß«‘∏’ ¥—ßπ’È

1. »÷°…“™à«ß§«“¡‡¢â¡¢âπ¢Õß “√≈–≈“¬

¡“µ√∞“π°√¥‡∫π‚´Õ‘°·≈–°√¥´Õ√å∫‘°„πµ—«Õ¬à“ß

‡§√◊ËÕߥ◊Ë¡ ‚¥¬§«“¡‡¢â¡¢âπ°√¥‡∫π‚´Õ‘° 5 §«“¡‡¢â¡¢âπ

(2.86-28.6 µg/ml) ·≈–™à«ß»÷°…“ linearity §◊Õ

0.0251-25.1 µg/ml ·≈–§«“¡‡¢â¡¢âπ°√¥´Õ√å∫‘° 5

§«“¡‡¢â¡¢âπ (2.83-28.3 µg/ml) ·≈–™à«ß»÷°…“

linearity §◊Õ 0.0260-26.0 µg/ml ∑—ÈßÀ¡¥ 7 ´È”

∑”°“√∑¥ Õ∫µ“¡«‘∏’ Compendium of Methods

for Food Analysis. 1st ed. 2003. Chapter1

2. »÷°…“À“¢’¥®”°—¥„π°“√µ√«®æ∫ (limit of

detection, LOD) ·≈–¢’¥®”°—¥∑’Ë “¡“√∂µ√«®À“

ª√‘¡“≥‰¥â (limit of quantitation, LOQ) ‚¥¬«‘‡§√“–Àå

10 È” ·≈–§”π«≥À“ à«π‡∫’ˬ߇∫π¡“µ√∞“π (SD)

‡æ◊ËÕÀ“§à“ LOD ·≈– LOQ ‚¥¬§”π«≥®“° LOD =

3*SD0 ·≈– LOQ = 10*SD03. »÷°…“¢’¥®”°—¥∑’Ë “¡“√∂µ√«®À“ª√‘¡“≥‰¥â

(LOQ) ‡æ◊ËÕ¬◊π¬—π LOQ ‚¥¬‡¡◊ËÕ‰¥â§à“ LOQ ®“°°“√

§”π«≥µ“¡¢âÕ 2 ·≈â«„Àâ∑” spike sample blank ∑’Ë

™à«ß§«“¡‡¢â¡¢âππ—Èπ (°√¥‡∫π‚´Õ‘° spike ∑’Ë√–¥—∫

§«“¡‡¢â¡¢âπ 0.502 µg /ml °√¥´Õ√å∫‘° ∑’Ë√–¥—∫

§«“¡‡¢â¡¢âπ 0.520 µg /ml) ·≈–∑”°“√«‘‡§√“–Àå

10 È” ·≈–§”π«≥À“§«“¡·¡àπ (accuracy) ·≈–

§«“¡‡∑’Ë¬ß (precision) ‚¥¬∑’ˇ°≥±å∑’ˬա√—∫‰¥â §◊Õ

%Recovery = 80-110 %

Õ“À“√·≈–™π‘¥¢Õß«—µ∂ÿ°—π‡ ’¬∑’ˇµ‘¡≈߉ª «‘∏’∑’Ëπ‘¬¡„™â

‰¥â·°à «‘∏’¡“µ√∞“πµ“¡ AOAC (2005) Method

960.38 ·≈–«‘∏’µ“¡¡“µ√∞“π Compendium of

Methods for Food Analysis (2003) ‚¥¬«‘∏’µ“¡

AOAC π—Èπ‡ªìπ°“√«‘‡§√“–Àå‚¥¬°“√ °—¥¥â«¬Õ’‡∏Õ√å

·≈–µ√«®«—¥À“ª√‘¡“≥«—µ∂ÿ°—π‡ ’¬¥â«¬‡§√◊ËÕß UV-VIS

spectrophotometer ´÷ËßµâÕß„™â “√≈–≈“¬„π°“√ °—¥

µ—«Õ¬à“߇ªìπ®”π«π¡“° ‡ªìπÕ—πµ√“¬µàÕºŸâªØ‘∫—µ‘ß“π

·≈– ‘Ëß·«¥≈âÕ¡ ‰¡à “¡“√∂∑¥ Õ∫ “√∑—Èß 2 ™π‘¥

„π§√“«‡¥’¬«°—π‰¥â µâÕß·¬°∑¥ Õ∫·µà≈–™π‘¥∑”„Àâ

µâÕß„™â‡«≈“¡“°„π°“√∑¥ Õ∫ ‚¥¬‡©æ“–Õ¬à“߬‘Ëß„π

‡§√◊ËÕߥ◊Ë¡∑’Ë¡’°“√‡µ‘¡«—µ∂ÿ°—π‡ ’¬∑—Èß Õß™π‘¥ à«π«‘∏’

¡“µ√∞“πµ“¡ Compendium of Methods for Food

Analysis π—È𠇪ìπ°“√«‘‡§√“–ÀåÀ“ª√‘¡“≥°√¥‡∫π‚´Õ‘°

·≈–°√¥´Õ√å∫‘° ¥â«¬‡§√◊ËÕß High Performance Liquid

Chromatograph (HPLC) ‚¥¬π”µ—«Õ¬à“ß¡“µ°µ–°Õπ

“√Õ◊Ëπ·≈â«·¬° “√≈–≈“¬„ ·≈–𔉪«‘‡§√“–ÀåÀ“

ª√‘¡“≥¥â«¬‡§√◊ËÕß HPLC µ√«®À“æ’§¢Õß°√¥‡∫π‚´Õ‘°

·≈–°√¥´Õ√å∫‘° ·≈–§”π«≥À“ª√‘¡“≥‚¥¬‡ª√’¬∫‡∑’¬∫

°—∫°√“ø “√¡“µ√∞“π¢Õß°√¥‡∫π‚´Õ‘° ·≈–°√¥´Õ√å∫‘°

(¿“æ∑’Ë 3) °“√π”«‘∏’¡“µ√∞“πÀ√◊Õ«‘∏’¥—¥·ª≈ß¡“„™â„π

ÀâÕߪؑ∫—µ‘°“√®–µâÕß¡—Ëπ„®«à“«‘∏’π—Èπ Ê „Àâº≈°“√∑¥ Õ∫

∑’Ë∂Ÿ°µâÕß·≈–πà“‡™◊ËÕ∂◊Õ ®÷ß®”‡ªìπµâÕß¡’°“√µ√«® Õ∫

§«“¡„™â‰¥â¢Õß«‘∏’À√◊Õ∑«π Õ∫§«“¡„™â‰¥â¢Õß«‘∏’

¿“æ∑’Ë 3 °√“ø‚§√¡“‚∑·°√¡¢Õß°√¥‡∫π‚´Õ‘°·≈–

°√¥´Õ√å∫‘°∑’ˉ¥â®“°‡§√◊ËÕß HPLC

36

º≈°“√∑¥≈Õß

1. ®“°°“√∑¥ Õ∫À“ª√‘¡“≥™à«ß§«“¡‡¢â¡¢âπ

¢Õß “√≈–≈“¬¡“µ√∞“π°√¥‡∫π‚´Õ‘° ·≈–°√¥´Õ√å∫‘°

§à“ —¡ª√– ‘∑∏‘Ï À —¡æ—π∏å æ∫«à“¡’§«“¡‡ªìπ‡ âπµ√ß À√◊Õ

Linearity ∑’Ë 0.0251-25.1 µg/ml ”À√—∫°√¥‡∫π‚´Õ‘°

·≈–æ∫§«“¡‡ªìπ‡ âπµ√ßÀ√◊Õ Linearity ∑’Ë 0.026-26.0

µg/ml ”À√—∫´Õ√å∫‘°¥—ß· ¥ß„π¿“æ∑’Ë 4 ·≈– 5

¿“æ∑’Ë 4 °√“ø§«“¡ —¡æ—π∏凙‘߇ âπ¢Õß°√¥‡∫π‚´Õ‘°

„πµ—«Õ¬à“ß°—∫æ◊Èπ∑’Ë„µâ°√“ø

¿“æ∑’Ë 5 °√“ø§«“¡ —¡æ—π∏凙‘߇ âπ¢Õß°√¥´Õ√å∫‘°

„πµ—«Õ¬à“ß°—∫æ◊Èπ∑’Ë„µâ°√“ø

2. ®“°°“√»÷°…“¢’¥®”°—¥„π°“√µ√«®æ∫

(LOD) ·≈–¢’¥®”°—¥∑’Ë “¡“√∂µ√«®À“ª√‘¡“≥‰¥â

(LOQ) ‚¥¬°“√À“ LOD „™â spike °√¥‡∫π‚´Õ‘°∑’Ë

√–¥—∫§«“¡‡¢â¡¢âπ 2.86 µg/ml ·≈–°√¥´Õ√å∫‘°∑’Ë

√–¥—∫§«“¡‡¢â¡¢âπ 2.83 µg/ml æ∫«à“ °√¥‡∫π‚´Õ‘°

¡’§à“ LOD ‡∑à“°—∫ 1.40 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡ °√¥´Õ√å∫‘°

¡’§à“ LOD ‡∑à“°—∫ 1.65 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡ ·≈–

°“√À“ LOQ „™â spike °√¥‡∫π‚´Õ‘°∑’Ë√–¥—∫§«“¡

‡¢â¡¢âπ 5.02 mg/kg (§«“¡∫√‘ ÿ∑∏‘Ï¢Õß°√¥‡∫π‚´Õ‘°

‡∑à“°—∫ ? %) ·≈–°√¥´Õ√å∫‘°∑’Ë√–¥—∫§«“¡‡¢â¡¢âπ

5.20 mg/kg (§«“¡∫√‘ ÿ∑∏‘Ï¢Õß°√¥´Õ√å∫‘°‡∑à“°—∫

98.5%) æ∫«à“°√¥‡∫π‚´Õ‘°¡’§à“ LOQ ‡∑à“°—∫ 4.64

¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡ °√¥´Õ√å∫‘°¡’§à“ LOQ ‡∑à“°—∫

5.52 ¡‘≈≈‘°√—¡µàÕ°‘‚≈°√—¡

3. ®“°°“√»÷°…“§«“¡·¡àπ (accuracy)

§«“¡‡∑’Ë¬ß (precision) ·≈–√âÕ¬≈–°“√‰¥â°≈—∫§◊π

(%Recovery) ®“°µ—«Õ¬à“ß∑’Ë¡’°“√‡µ‘¡ “√≈–≈“¬

¡“µ√∞“π‡∫π‚´Õ‘° 0.502 ‰¡‚§√°√—¡µàÕ¡‘≈≈‘≈‘µ√‰¥â

%Recovery ‡∑à“°—∫ 103.52 ´÷ËßÕ¬Ÿà„π™à«ß 80-110%

·≈– %RSD / RSDr πâÕ¬°«à“ 2 ·≈–∑¥ Õ∫µ—«Õ¬à“ß

∑’Ë¡’°“√‡µ‘¡ “√≈–≈“¬¡“µ√∞“π´Õ√å∫‘° 0.520 ‰¡‚§√°√—¡

µàÕ¡‘≈≈‘≈‘µ√ %Recovery ‡∑à“°—∫ 98.29 ´÷ËßÕ¬Ÿà„π™à«ß

80-110% ·≈– %RSD / RSDr πâÕ¬°«à“ 2 ¥—ßπ—Èπ

∑’˧«“¡‡¢â¡¢âπ√–¥—∫π’È¡’ Accuracy ·≈– Precision ¥’

(µ“¡µ“√“ß∑’Ë 1)

37

°“√∑¥ Õ∫À“ª√‘¡“≥°√¥‡∫π‚´Õ‘° ·≈–

°√¥´Õ√å∫‘°‚¥¬„™â°“√µ°µ–°Õπ·≈–µ√«®«‘‡§√“–Àå

¥â«¬‡§√◊ËÕß HPLC ‡ªìπ«‘∏’°“√∑’ˇÀ¡“– ¡µàÕµ—«Õ¬à“ß

ª√–‡¿∑∑’ˇªìπ‡§√◊ËÕߥ◊Ë¡·≈–¢Õ߇À≈« ·≈–‡À¡“– ¡

µàÕµ—«Õ¬à“ß∑’ËÕ“®¡’∑—Èß°√¥‡∫π‚´Õ‘° ·≈–°√¥´Õ√å∫‘°

º ¡Õ¬Ÿà∑—Èß Õß™π‘¥ ‡π◊ËÕß®“°°“√„™â«‘∏’∑¥ Õ∫«‘∏’Õ◊Ëππ—Èπ

°“√Õà“πº≈¢Õß°√¥´Õ√å∫‘°®–¡“√∫°«πº≈°“√∑¥ Õ∫

√ÿªº≈·≈–°“√«‘®“√≥åº≈

®“°°“√∑¥ Õ∫§«“¡„™â‰¥â¢Õß«‘∏’‚¥¬„™â‡§√◊ËÕߥ◊Ë¡

‡ªìπµ—«Õ¬à“ß„π°“√∑¥ Õ∫ ‚¥¬»÷°…“æ“√“¡‘‡µÕ√åµà“ß Ê

¥—ßπ’È §◊Õ ™à«ß§«“¡‡¢â¡¢âπ∑’Ë„Àâ°√“ø‡ªìπ‡ âπµ√ß §«“¡

—¡æ—π∏凙‘߇ âπ¢’¥®”°—¥„π°“√µ√«®æ∫ ¢’¥®”°—¥∑’Ë “¡“√∂

µ√«®À“ª√‘¡“≥‰¥â §«“¡‡∑’Ë¬ß §«“¡·¡àπ æ∫«à“

§à“µà“ß Ê Õ¬Ÿà„π‡°≥±å∑’ˬա√—∫‰¥â

°√¥‡∫π‚´Õ‘° °√¥´Õ√å∫‘°µ—«Õ¬à“ß

§à“∑’Ë spike 2.86 µg/ml §à“∑’Ë spike 5.02 mg/kg §à“∑’Ë spike 2.83 µg/ml §à“∑’Ë spike 5.20 mg/kg

1 2.6352 4.8820 2.6647 4.9649

2 2.7455 4.9161 2.7685 4.8469

3 2.6700 4.9141 2.6244 4.9006

4 2.7592 4.9540 2.6106 4.9121

5 2.6913 5.1204 2.7415 4.9852

6 2.7240 5.2182 2.7163 4.9942

7 2.6735 5.1615 2.6714 5.0847

8 2.7609 5.0478 2.6972 5.0262

9 2.6938 5.1423 2.6148 4.8463

10 2.7719 4.9019 2.7319 4.8697

average 2.7125 5.0258 2.6841 4.9431

SD 0.0464 0.1265 0.0560 0.0806

3SD µ—¥ÕÕ° 0.1392 0.3795 0.1680 0.2418

10SD µ—¥ÕÕ° 0.4640 1.265 0.5599 0.8060

%RSD 1.71 2.5181 2.09 1.6322

%Recovery 94.84 103.52 ????? 98.29

RSDr 9.02 11.71 9.02 11.65

%RSD /

RSDr0.19 0.21 0.23 0.14

µ“√“ß∑’Ë 1 ¢âÕ¡Ÿ≈°“√∑¥ Õ∫À“§«“¡∂Ÿ°µâÕß·≈–·¡àπ¬”¢Õß°“√∑¥ Õ∫À“°√¥‡∫π‚´Õ‘°·≈–°√¥´Õ√å∫‘°

38

¢Õß°√¥‡∫π‚´Õ‘° ∑”„Àâ‰¡à “¡“√∂∑√“∫§à“∑’Ë·∑â®√‘߉¥â

©–π—Èπ„π°“√∑¥ Õ∫À“°√¥‡∫π‚´Õ‘°·≈–°√¥´Õ√å∫‘°

π—°«‘∑¬“»“ µ√å®–¡’§«“¡™”π“≠„π°“√∑¥ Õ∫·≈–

µâÕß¡’°“√‡≈◊Õ°«‘∏’∑¥ Õ∫∑’ˇÀ¡“– ¡°—∫µ—«Õ¬à“ß™π‘¥

µà“ß Ê ‰¥â ‡æ◊ËÕ„À≥âº≈°“√∑¥ Õ∫∑’Ë√«¥‡√Á«·≈–∂Ÿ°µâÕß

‚§√ß°“√«‘∑¬“»“ µ√å™’«¿“æ °√¡«‘∑¬“»“ µ√å

∫√‘°“√ ‡ªìπÀπ૬ߓπ∑’Ë„Àâ∫√‘°“√«‘‡§√“–Àå∑¥ Õ∫

°√¥‡∫π‚´Õ‘°·≈–°√¥´Õ√å∫‘°„πº≈‘µ¿—≥±åÕ“À“√·≈–

‡§√◊ËÕߥ◊Ë¡∑’Ë ‰¡à¡’·Õ≈°ÕŒÕ≈å ¥â«¬«‘∏’ UV-VIS

Spectrophotometer ·≈–«‘∏’ HPLC ∑’ˉ¥â√—∫°“√√—∫√Õß

§«“¡ “¡“√∂ÀâÕߪؑ∫—µ‘°“√µ“¡¡“µ√∞“π ISO/IEC

17025 : 2005 ®“° ”π—°√—∫√Õß¡“µ√∞“πÀâÕߪؑ∫—µ‘°“√

°√¡«‘∑¬“»“ µ√å°“√·æ∑¬å °√–∑√«ß “∏“√≥ ÿ¢

πÕ°®“°π’ȉ¥â‡¢â“√à«¡°‘®°√√¡∑¥ Õ∫§«“¡™”π“≠ ∑—Èß

®“°Àπ૬ߓπ„πª√–‡∑»·≈–µà“ߪ√–‡∑»∑’ˇªìπ∑’Ëπà“‡™◊ËÕ∂◊Õ

‡™àπ °√¡«‘∑¬“»“ µ√å°“√·æ∑¬å ∂“∫—π«‘®—¬«‘∑¬“»“ µ√å

·≈–‡∑§‚π‚≈¬’·Ààߪ√–‡∑»‰∑¬¿“¬„µâ‡§√◊Õ¢à“¬¡“µ√

«‘∑¬“‡§¡’ ·≈– FAPAS ‡æ◊ËÕ‡ªìπ°“√¬◊π¬—𧫓¡ “¡“√∂

¢ÕßÀâÕߪؑ∫—µ‘°“√„π°“√µ√«®«‘‡§√“–Àå “√¥—ß°≈à“«

„Àâ¡’§«“¡πà“‡™◊ËÕ∂◊Õ·≈–‡ªìπ∑’ˬա√—∫¢Õß≈Ÿ°§â“∑’Ë¡“

„™â∫√‘°“√°—∫ÀâÕߪؑ∫—µ‘°“√¢Õß°√¡«‘∑¬“»“ µ√å∫√‘°“√

‡Õ° “√Õâ“ßÕ‘ß

JECFA. Combined Compendium of Food Additive Specifications, Monograph 1, 2006. [Online]. [cited

28 July 2010]. Available from Internet : http://www.fao.org/ag/agn/jecfa-additives/search.html.

°√–∑√«ß “∏“√≥ ÿ¢. ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“. ª√–°“»°√–∑√«ß “∏“√≥ ÿ¢ ©∫—∫∑’Ë 84 æ.». 2527.

‡√◊ËÕß«—µ∂ÿ‡®◊ÕªπÕ“À“√. °√ÿ߇∑æœ: ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“, 2527.

_______. ª√–°“»°√–∑√«ß “∏“√≥ ÿ¢ ©∫—∫∑’Ë 214 æ.». 2543 ‡√◊ËÕß ‡§√◊ËÕߥ◊Ë¡„π¿“™π–∫√√®ÿ∑’Ëªî¥ π‘∑.

°√ÿ߇∑æœ: ”π—°ß“π§≥–°√√¡°“√Õ“À“√·≈–¬“, 2543.

_______. ª√–°“»°√–∑√«ß “∏“√≥ ÿ¢ ©∫—∫∑’Ë 281 æ.».2547 ‡√◊ËÕß«—µ∂ÿ‡®◊ÕªπÕ“À“√. °√ÿ߇∑æœ: ”π—°ß“π

§≥–°√√¡°“√Õ“À“√·≈–¬“, 2547.

æÿ∑∏√‘π∑√å «√√≥‘ √. “√°—π∫Ÿ¥. [ÕÕπ‰≈πå]. [Õâ“ß∂÷ß«—π∑’Ë 28 ‘ßÀ“§¡ 2551]. ‡¢â“∂÷߉¥â®“°Õ‘π‡∑Õ√å‡πÁµ :

http://guru.sanook.com/pedia/topic/

¡À“«‘∑¬“≈—¬‡°…µ√»“ µ√å. «—µ∂ÿ‡®◊ÕªπÕ“À“√ ‡≈à¡ 1. ‚¥¬ »‘«“æ√ »‘«‡«™. °√ÿ߇∑æœ: ‚√ßæ‘¡æ廟π¬å à߇ √‘¡·≈–

Ωñ°Õ∫√¡°“√‡°…µ√·Ààß™“µ‘, 2546, Àπâ“ 11-36.

”π—°¡“µ√∞“πº≈‘µ¿—≥±åÕÿµ “À°√√¡. ‡√◊ËÕßπÈ”À«“π‡¢â¡¢âπ. ¡Õ°.155-2518, 11 Àπâ“.