RNAi

MITOCHONDRIA

C P#1 P#2

WB: p53

WB: VDAC

WB: PCNA

WB: Pin1

Supplementary Figure 2A

B

TOTAL MITOTOTAL MITO

C Pin1RNAi

+– +– +– +–Etoposide

WB: p53

WB: VDAC

WB: PCNA

EtoposideC Pin1

ACTIN

Pin1

RNAi– + +–

WB: CLEAVED CASPASE-3

WB: Pin1

WB: p53

WB: ACTIN

Thymus Bone Marrow

5Gy γ-IR Pin1 +/+

+++++/+ –/– –/–

C

D

IVTT MBPp53GST Pin1GST WWGST

ISOMERIZATION

WASHGST BclXL

SDS PAGE

GST

GST BclXL

INPUT

MBPMBP p53

WB: GST

WB: MBP

Pin1 Pin1WW WWGST

WB: p53

WB: VDAC

WB: PCNA

WB: Pin1

+– +–Doxorubicin

TOTAL MITO

Doxorubicin + + +

IP: p53

p53 WT S46A

++– –+ + + +HA-Ub

Pin1 –+–

WB: p53

WB: HA

WB: PIN1

WB: actin

WB: p53

inputs

– monoUb

E

HA

/p5

3