RNAi
1
RNAi MITOCHONDRIA C P#1 P#2 WB: p53 WB: VDAC WB: PCNA WB: Pin1 pplementary Figure 2 A B TOTAL MITO TOTAL MITO C Pin1 RNAi + – + – + – + – Etoposide WB: p53 WB: VDAC WB: PCNA Etoposide C Pin1 ACTIN Pin1 RNAi – + + – WB: CLEAVED CASPASE-3 WB: Pin1 WB: p53 WB: ACTIN Thymus Bone Marrow 5Gy γ-IR Pin1 +/+ + + + + +/+ –/– –/– C D IVTT MBPp53 GST Pin1 GST WW GST ISOMERIZATION WASH GST BclXL SDS PAGE GST GST BclXL INPUT MBP MBP p53 WB: GST WB: MBP Pin1 Pin1 WW WW GST WB: p53 WB: VDAC WB: PCNA WB: Pin1 + – + – Doxorubicin TOTAL MITO Doxorubicin + + + IP: p53 p53 WT S46A + + – – + + + + HA-Ub Pin1 – + – WB: p53 WB: HA WB: PIN1 WB: actin WB: p53 inputs – monoUb E HA/p53
description
Supplementary Figure 2. A. TOTAL. MITO. C. Pin1. RNAi. C. Pin1. RNAi. Doxorubicin. MITO. TOTAL. –. +. –. +. MITO. TOTAL. Etoposide. –. +. –. +. –. +. –. +. –. +. –. +. WB: p53. Pin1. WB: p53. WB: Pin1. ACTIN. WB: VDAC. WB: VDAC. WB: PCNA. WB: PCNA. D. B. - PowerPoint PPT Presentation
Transcript of RNAi
RNAi
MITOCHONDRIA
C P#1 P#2
WB: p53
WB: VDAC
WB: PCNA
WB: Pin1
Supplementary Figure 2A
B
TOTAL MITOTOTAL MITO
C Pin1RNAi
+– +– +– +–Etoposide
WB: p53
WB: VDAC
WB: PCNA
EtoposideC Pin1
ACTIN
Pin1
RNAi– + +–
WB: CLEAVED CASPASE-3
WB: Pin1
WB: p53
WB: ACTIN
Thymus Bone Marrow
5Gy γ-IR Pin1 +/+
+++++/+ –/– –/–
C
D
IVTT MBPp53GST Pin1GST WWGST
ISOMERIZATION
WASHGST BclXL
SDS PAGE
GST
GST BclXL
INPUT
MBPMBP p53
WB: GST
WB: MBP
Pin1 Pin1WW WWGST
WB: p53
WB: VDAC
WB: PCNA
WB: Pin1
+– +–Doxorubicin
TOTAL MITO
Doxorubicin + + +
IP: p53
p53 WT S46A
++– –+ + + +HA-Ub
Pin1 –+–
WB: p53
WB: HA
WB: PIN1
WB: actin
WB: p53
inputs
– monoUb
E
HA
/p5
3
