Platelet rich plasma

Preethi chekuri

What is Platelet Rich Plasma?

It is an effective concentration of multiple fundamental growth factors (GF’s) by virtue of platelets alone (stored as α-granules in platelets) and plasma proteins

“REGENERATIVE”

• Degranulation of the pre-packed GF’s in platelet occurs upon “activation”

• COCKTAIL of growth factors

• Cacl2 and calcium gluconate(1 in 10 dilution)

Manual double spin method:

Soft spin – 1200 rot/min for 10 min Hard spin – 2400 rot/min for 10 min

PRP preparation

• Aim : to sequestrate platelets in high concentrations, enough for achieving therapeutic benefit and in a viable state at the same time, so that they can actively secrete their GF’s

• Precautions : aseptic condition

optimum temp regulation

anticoagulant

Indications for use

• Androgenic alopecia• Alopecia areata• Acne scars • Skin rejuvenation• Wound healing

Procedure

• A special dermaroller is used to stimulate dermis of scalp.

• The microneedling causes microtrauma to dermis that induces growth repair cells into action.

• PRP is then applied topically

Mechanism of action

Differentiation of hair follicle stem cellsbcl-2 : anti apoptotic – prolonged

survival of dermal papilla cellsExpression of FGF-7 : prolonged anagenVEGF and PDGF - proangiogenic

Modes of usage

• PRP mesotherapy• Used in conjunction with minoxidil daily

topical therapy• Used as an adjunct to hair transplantation

(it is noted to increase the result)

Safety

• It is likely that hundreds of thousands of humans have been treated in clinical practice by now

• No major side effects reported• AUTOLOGOUS – minimum risk

of reactivity compared to exogenous compounds

In vitro research

• PRP increased in vitro proliferation and differentiation of stem cells in the hair follicle bulge area and has an angiogenic role which makes it useful as a hair growth modality

• Thrombin and CaCl2 increased GF release in dose dependent manner in-vitro

In vivo research

• Wide variability between patients in PRP content

• Variability between PRP ‘resting’ and ‘activation’

Various studies

Conclusion

PRP has been known and used in dermatology, dentistry and sports medicine

The mechanism of action of GFs have been studied and well established

So this technology should not be considered experimental any more, although it can still undergo further improvement in the furure

Thank you