Post on 14-Jan-2016
description
Supplementary Figure 1. Methylation level of 4 CpG sites located in the CYP24A1 promoter and gene in 33 nonmalignant lung samples and 153 lung AC included in the CURELUNG study. Non-malignant samples had a significantly higher β-value (p < 0.05, Wilcoxon rank sum test) for these CpG sites as compared to lung AC.
0 1
0.06
0.08
0.10
0.12
0.14
0 1
0.08
0.10
0.12
0.14
0.16
0 1
0.10
0.15
0.20
0.25
0.30
0 1
0.65
0.70
0.75
0.80
0.85
0.90
Non-malignant Tumors samples
Non-malignant Tumors samples
Non-malignant Tumors samples
Non-malignant Tumors samples
DN
A m
eth
ylat
ion
lev
els
(β)
cg02673418 cg02604290 cg24582168p < 0.0001 p = 0.005 p = 0.0006
cg24849555
p = 0.01
TSA(nM)
1,25-D3
5-Aza(M)
A B
1,25-D3
A549H460
SKLU-1
DNMT1
DNMT3A
GAPDH
C
Supplementary Figure 2. (A) Quantitative RT-PCR of CYP24A1 expression in H460 and SK-LU-1 cells treated with increasing concentrations of 5-Aza followed by 100 nM of 1a,25-dihydroxyvitamin D3 (1,25-D3). (B) Quantitative RT-PCR of CYP24A1 expression in H460 and SK-LU-1 cells treated with increasing concentrations of TSA followed by 100 nM of 1,25-D3. Barplots and error bars represent mean and standard deviation of triplicate data points. (C) Basal protein expression of DNMT1 and DNMT3A in A549, NCI-H460 and SK-LU-1 cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control.