Asni Ramdani

 

Haemoglobin, Mioglobin and Binding

Mechanism of Oxygen

November, 29, 2011

O2 CARRIER

HAEMOGLOBINMIOGLOBI

N

HEMOGLOBIN

Hemoglobin A (HbA)

Hemoglobin

4 globular protein

4Heme Group

2 sub unit α2 sub unit β

HEMOGLOBINThe totality of amino acid in hemoglobin :

• Sub unit α : 141 residue

• Sub unit β : 146 residue

STRUKTUR HEM

THE FIFTH AND SIXTH COORDINATION POSITION OF HEME

N NFe

N N

1

4 3

2

65

1 2

6

5

34

GLOBIN

BINDING HEME BY GLOBIN

BINDING SCHEME

BINDING OF OXIGEN CAUSE STRUCTURAL CHANGES

MODEL BERURUTAN

MODEL SEREMPAK

PENGIKATAN 2,3-BPG PADA HEMOGLOBIN

MANUSIA

2,3-BISFOSFOGLISERAT / 2,3-BPG /

2,3-DIFOSFOGLISERAT / 2,3-DPG

BPG DECREASE THE BOUNDED OF O2

As stabilisator of deoksihemoglobin status (T status)

Created 1 more “jembatan garam”

OXYGENATION IS REGULATED BY BPG

STRUCTURE OF MYOGLOBIN

Outline of Bohr effect

CO2 + H2O H2CO3, H2CO3 unravel into proton and ions bikarbonat.In the lungs,  binding of oxygen by hemoglobin will release protons from hemoglobin.