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Page 1: Supplemental Figure 1. The lateral root number in …...2015/05/22  · Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091 1 Supplemental Figure 1. The lateral

Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 1. The lateral root number in WT, brm-1, brm-3, and

brm-5 roots at 10 DAG.

Supplemental Figure 2. The expression of CycB1;1 and CycB1;3 in 3 DAG WT,

brm-3 and brm-5 roots. Bar=50 μm.

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Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 3. Cellular organization of WT (Col-0), brm-3, brm-5 and

brm-1 root tips at 3 DAG using propidium iodide staining (A) and the length of

4th columella cell of the WT (Col-0), brm-3 and brm-5 root tips at 3 DAG (B).

The QC (white triangle) and columella cell areas (white asterisk) are indicated.

Bars = 20 μm. The 4th columella cell of brm-1 root tips could not be identified.

Data shown are means ± SD (n =20).

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Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 4. The expression of PIN2 in WT (Col-0) and brm-3 root

tips at 3 DAG (A) and the expression of root development related genes in WT

(Col-0) and brm-1 5-day-old seedlings. Bars = 20 μm.

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Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 5. The expression pattern of SHR and SCR in WT and

brm-3 (A, C) and the phenotype of shr-1 brm-3 (B), scr-1 brm-3 (D), shr-1

brm-1 (E) and scr-1 brm-1 (F) double mutants. Bars = 20 μm (A, C) and 1 cm (B,

D, E, F).

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Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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Supplemental Figure 6. The phenotype of plt1-4 plt2-2 brm-3 (A) and plt1-4

plt2-2 brm-1 (B) triple mutants at 7 DAG. Bars = 1 cm.

Supplemental Figure 7. The phenotype of Col-0 and BRMpro:BRM:GFP/brm-1

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seedlings at 10 DAG . Bars = 0.5 cm.

Supplemental Figure 8. ChIP-qPCR analysis of BRM targeting to PIN3, PIN4

and PIN7 (A) and H3K27me3 levels of PIN3, PIN4 and PIN7 loci in brm-3

mutant roots (B). Data are mean values ± standard deviation of three

replicates. Similar results were obtained for at least two additional independent

experiments. Asterisks denote Student’s t- test significant difference between

Col-0 and BRMpro: BRM:GFP/Col-0 roots (p<0.05).

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Supplemental Figure 9. BRM does not target to PLT1 and PLT2 directly.

Supplemental Figure 10. The normal expression of QC25 and QC46 in WT

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Supplemental Data. Yang et al. (2015). Plant Cell 10.1105/tpc.15.00091

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(Col-0) and brm-3 root tips at 3 DAG. Bars = 25 μm.

Supplemental Table 1. Primers used for RT-qPCR

Primer

name

Forward Reverse

PLT1 AGTTCGTGGCTGCCATTAGAAGG ATCTTCCATGTTGGTGATGCCTTG

PLT2 GAAGATGGCAAGCAAGGATCGG GCTTCTTCCTCCGTGCTGAATG

PIN1

GGCATGGCTATGTTCAGTCTTGG

G

ACGGCAGGTCCAACGACAAATC

PIN2

TCACGACAACCTCGCTACTAAAG

C

TGCCCATGTAAGGTGACTTTCCC

PIN3 AAGGCGGAAGATCTGACCAAGG TGCTGGATGAGCTACAGCTTTG

PIN4 ACAACGTGGCAACGGAACAATC GCCGATATCATCACCACCACTC

PIN7 CGTGTGGCCATTGTTCAAGCTG

CCCTGTACTCAAGATTGCGGGAT

G

CYB1;1 CACTATTTGGCTGAGTTAGG ATCTTGCTGCGTAGATTGC

CYB1;3 TACTCGTAACTTTCGTGCTCAG GAACCGCTCTTACAACTTCTTG

UBQ10 TCGACCCTTCACTTGGTGTTGC

GGGTGATGGTCTTTCCGGTCAAA

G

Supplemental Table 2. Primers used for ChIP-qPCR

Primer

name

Forward Reverse

Length of

amplicon

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PLT1-P1 gaggacgtgagttcccataatc gcaaattaattatcactcacaatg 132 bp

PLT1-P2 AGGAGAGGTTCCAAAAGTGGCC ATTGGACGCTAGGCATCAAG 131 bp

PLT1-P3 ttccacttaattaggttttgtc ttatacgattcgatgcactcac 150 bp

PLT1-P4 TGGCTGCCATTAGAAGgttt GATACATCGAAGCGCCTCTC 140 bp

PLT1-P5 GGCTCCACCTCATCAAGACT AAGAGGAGGAATCGTGAGCA 133 bp

PLT1-P6 tcaatttagagggggccttt cccataaaaccctaaccctca 120 bp

PLT2-P1 taaacgagggacgtgagtt cgcttccaactagaaaaagtc 130 bp

PLT2-P2 ccaagaaaagggaaATGAA GGTTTTGAAAAGGGTTGTCG 148 bp

PLT2-P3 CAGAAGTCGCCACTGTGAAA CCAAAAGTTTCCAAAGTCCGT 124 bp

PLT2-P4 tcggactatgcactcagGTG tcatgaaaatttgaaataacttacCG 130 bp

PLT2-P5 AACGGCTCAAAGAAGCTCAA AGAGCTGGAAGCAACAGAGC 122 bp

PLT2-P6 acaagtattaggatcggctgga ccctcgaggactctgataacc 120 bp

PIN1-P1 gggataaattgatcaaccgaat ggtggagctttggggatagt 140 bp

PIN1-P2 ttccctcttcaccacttctctc ACGGAACCATAGCCGTCATA 128 bp

PIN1-P3 GAACCCAGGGATGTTTTCG GCACTTGAGCTCCACACAAA 135 bp

PIN1-P4 CCTCCATGTTGCCATTATCC AAGCTGCctgtttgttgtca 125 bp

PIN1-P5 tgccctttttgttgaaaacc aaggtaaaacttgctgagctcct 127 bp

PIN2-P1 ccaatcttccttatcccaaga ggtccataatttttcgaagtgc 137 bp

PIN2-P2 tctctcgccggaaaaagtaa GTGTGAATATCCCCCACCAC 132 bp

PIN2-P3 TCTCACCGGCGTAGAGATTT GCCGCCGTAGCTATTAGTGT 148 bp

PIN2-P4 ttaaatagatataagaatcgatt aattattattattttgttaaaaga 146 bp

PIN2-P5 ttgcaaataaaaggcgatacg attaatgcgacattgcatgg 128 bp

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PIN3-P1 aaaatcaagcacatgaatgtcac tcgactgattgaaacggaca 122 bp

PIN3-P2 cactgctcccaagtcctctc AGGATCATGGCCACGTAGAG 147 bp

PIN3-P3 AGGTCCAACTCCTCGACCTT GACGCTTTTATTGGCGGTAG 154 bp

PIN3-P4 TGGACTTATTTGGGCTCTCG GATTTTGGGCATTGCCACGTG 135 bp

PIN3-P5 acgcagataaggaggagcaa cccgaacctaatcaaagaaca 153 bp

PIN4-P1 gagttttctggagggacgtg cgtagtattcgccaaagttcc 129 bp

PIN4-P2 tcctcttcaccgaatccttg cggtgggttttggagtttag 144 bp

PIN4-P3 ctgagaaaaccaaaaatcatgaatac tcctcttcaccgaatccttg 120 bp

PIN4-P4 tgaccctctgacccagacat ggatttgagaaaattgacagca 127 bp

PIN4-P5 TCTTGGCCTTTGAattggtc cttccactctttcccacgaa 121 bp

PIN7-P1 aagttttggaccggcattta caatgaattttgtgaatccttga 140 bp

PIN7-P2 tgttaaaagcttccgtcatca AGGACGGTGTAGAGGTCGTG 140 bp

PIN7-P3 CATACTCAAAATGGTGAAAAC ATAGTCCCCGTTCATCGGAC 125 bp

PIN7-P4 ggtgggatgacatagctcgt aacaagtgctgaatgatgcaa 153 bp

PIN7-P5 tctgcttggctgaagaggat ggatcctcaaatcaaatcttgg 125 bp

TA3 ctgcgtggaagtctgtcaaa ctatgccacagggcagttt 106bp

TUB2

ACAAACACAGAGAGGAGTGAGC

A

ACGCATCTTCGGTTGGATGAG

TG

150 bp