complete viScoSity breaK acroSS extreme conditionS

EASY TO USE ENZYME BREAKER THAT IS SENSITIVE TO EQUIPMENT, RESERVOIR FLUIDS AND THE ENVIRONMENT

Pyrolase cellulase enzyme is a broad spectrum -glycosidase

which can be employed in a variety of industrial applications,

as it operates at broader pH ranges and much higher

temperatures than conventional enzymes.

Pyrolase cellulase excels in the breaking (or hydrolysis) of

linear and cross-linked fluids such as guar gum, derivatized

guar, and carboxymethyl cellulose. Safe to use, Pyrolase

cellulase offers an easily controlled break under a variety

of down-hole conditions. This enzyme product is a direct

replacement for corrosive acids and oxidizers, providing

superior performance with zero environmental impact.

product Summary

advantageS

EfficiEnt in thE

complEtE hydrolysis

of guar gum,

derivatized guar,

and carboxymethyl

celluloSe

Provides a complete viscosity break across a wide range of temperature, pH, and high salinity

Superior performance at extreme temperatures and pH compared to commercially available enzymes

Break time is easily controlled by varying enzyme loading

Effective at breaking linear and cross-linked fluids

Easy to use liquid formulation

Replaces hazardous chemicals, safe to use

No environmental impact, 100% bio-degradable

high Science SolutionS for high performance As a pioneer in ethical bio-prospecting

and bio-engineering, Verenium has a

heritage of developing best-in-class enzyme products with an approach grounded in scientifi c excellence.

At the heart of this technology is the ability to discover and extract enzymes from nature that

conventional approaches miss. An example of this is Pyrolase cellulase, which was found in the extreme

environment of a volcanic vent.

Pyrolase cellulase exemplifi es the high standard of Vereniums enzymatic innovations. This enzyme

breaker is renowned for its performance at high temperatures, pH and high salinity conditions

encountered in oil fi eld and gas well fracturing operations. Its selective and specifi c action for the

complete hydrolysis of guar-based fracturing fl uids confi rms its importance in improving viscosity

control in deep and high temperature wells. This means increased extraction and greater production

of oil and gas from existing wells. As a carefully tailored enzyme breaker, Pyrolase cellulase is by its

very nature selective in its action and thereby provides a complete, non-corrosive and environmentally

benign solution to gel-based fracturing jobs. It is high science for high performance to the benefi t of

more effi cient and cleaner processes.

operating range inactive

TEMPERATURE F (C)

COMPETITOR ENZYME

COMPETITOR ENZYME

80 100 120 140 160 180 200 22027 38 49 60 71 82 93 104

pH

4 5 6 7 8 9 10 11

APPLICATIONPyrolase cellulase enzyme is a broad spectrum -glycosidase with activity against guar, hydroxypropyl guar, carboxymethyl hydroxypropyl guar, and carboxymethyl cellulose. Pyrolase cellulase has been designed to cleave the 1,4 beta-linkage in these substrates and it is both endo- and exo-acting, allowing it to effectively reduce the viscosity of guar and derivatized guar solutions.

PACKAGE SIZES Container: 1 quart (946 ml) Packaged 4 containers per box

STORAGE AND STABILITY Recommended Storage Conditions: Store in tightly sealed containers

at 39 - 46 F (4 - 8 C) Shelf Life: Minimum of 1 year if stored at 39 - 46F (4 - 8C)

CONTACT INFORMATIONFor more information on

Pyrolase cellulase or other

Verenium enzymes, please contact:

Verenium Customer Service

3550 John Hopkins Ct.

San Diego, CA 92121

Phone: 1.800.523.2990 or

858.431.8500

Fax: 858.431.7273

(8 a.m. 5 p.m. PST)

www.verenium.com

enzymes@verenium.com

SpecificationS

The information contained herein is based on data considered accurate with representative samples. However, Verenium makes no warranty, express or implied, regarding the accuracy of these data, the use of the product, or the results to be obtained from the use thereof. The above data do not imply specification for this product. Verenium assumes no responsibility for personal injury, property damage, or other loss to vendees, users or third parties, caused by the product. Such vendees, users, or third parties assume any and all risks associated therewith. Consult the Materials and Safety Data Sheet before using the product. Nothing herein shall be construed as a recommendation for uses which would infringe the valid patent rights of third parties nor as extending an express or implied license under such patents and Verenium disclaims all liability therefor. Verenium, Pyrolase, the Verenium corporate logo, the Pyrolase logo, and The Energy of Nature are registered trademarks or trademarks of Verenium Corporation in the U.S. and/or other countries. All other company and product names and logos may be trademarks of their respective companies. Copyright 2012 Verenium Corporation.

V3-2012US Pat. 5,962,258, 6,008,032, 6,245,547 and other patents pending