Insights into Basic and Clinical Neurobiology Derived from the Analysis of Genetic causes of
Neurodegenerative Disease
P. St George-Hyslop
Centre for Research in Neurodegenerative Diseases,Toronto Western Hospital Research Institute,
University of Toronto,Toronto, Ontario, CANADA
Overview
• Genetics and Biology of Dementias– Alzheimer Disease:
• APP, PS1, PS2, APOE ε4• Other unidentified genes
– Fronto-temporal Dementia (& PSP , CBD)• Tau
– Dementia with Lewy Bodies• APOE ε4
• Current knowledge of known disease causing pathways;
• Application of current knowledge– Prediction of future risks, pharmacogenomics– Design of rational therapeutics
Emerging Concept: neurotoxic intra- or extra-cellular deposition of insoluble proteins (-sheet conformation) is
the cause of many neurodegenerative diseases
DiseaseDisease Protein Protein Enabling eventEnabling event
Alzheimer Disease Alzheimer Disease AAββ ( (ββAPP)APP) ββ- /- /γγ-secretase-secretaseFrontotemporal DementiaFrontotemporal Dementia TauTau ??Creutzfeldt-JacobCreutzfeldt-Jacob PrPSc (PrPc)PrPSc (PrPc) ??Familial EncephalopathyFamilial EncephalopathyNeuroserpinNeuroserpin ??Familial British DementiaFamilial British Dementia ABri (BRI)ABri (BRI) Furin cleavageFurin cleavageParkinson’s DiseaseParkinson’s Disease αα-synuclein-synuclein ??
What causes Alzheimer Disease?
• Genetic Factors (40% of attributable population risk):– Mutations in genes:
• Amyloid Precursor Protein (APP);• Presenilin 1 (PS1);• Presenilin 2 (PS2);• Apolipoprotein E (APOE ε4);• Other genes on other chromosomes.
• Environmental Factors (± genetic predispositions):– Evidence for specific environmental factors is not robust
• Lower childhood education• Head Injury• Cerebrovascular disease• ?Aluminium
Genetic and “non-genetic” cases are indistinguishable
• Genetic and non-genetic cases have identical:– Clinical features;– Brain pathology; – Brain biochemistry (increased brain levels of
Amyloid β-peptide (Aβ) and tau);– Mortality.
Genetic Determinants of Alzheimer’s Disease
Genetic Determinants of Alzheimer’s Disease
PresenileFamilial ADPresenile
Familial ADSenileSenile
Familial ADFamilial ADSenileSenile
Familial ADFamilial ADSporadicSporadic
ADADSporadicSporadic
ADAD
Presenilin 1Presenilin 1genegene
(chr 14)(chr 14)
age: 25–60 yrsage: 25–60 yrs
Presenilin 1Presenilin 1genegene
(chr 14)(chr 14)
age: 25–60 yrsage: 25–60 yrs
Presenilin 2Presenilin 2genegene
(chr 1)(chr 1)
45–84 yrs45–84 yrs
APPAPPgenegene
(chr 21)(chr 21)
40–65 yrs40–65 yrs
APPAPPgenegene
(chr 21)(chr 21)
40–65 yrs40–65 yrs
APOE APOE 4 allele4 allele(chr 19)(chr 19)
>50 yrs>50 yrs
APOE APOE 4 allele4 allele(chr 19)(chr 19)
>50 yrs>50 yrs
Other genes yet to be identifiedOther genes yet to be identifiedOther genes yet to be identifiedOther genes yet to be identified
The APP gene encodes a Type 1 membrane protien, a fragment of which accumulates in AD brain
Citron et al. Nature Med. 3: 67-72, 1997
APPCell membrane
Aβ peptide domain
Physiological Endo-proteolytic Processing of APP
-secretase -secretase
Aβ40 >> Aβ42
AICD(?Signalling)
-secretase
Uptake, chaperoning, °radation of Aβ by neprilysin, IDE, others
APP
Cell membrane
Transcriptional induction
Citron et al. Nature Med. 3: 67-72, 1997Pardossi-Piquard R et al. Neuron 46:541-554, 2005.
Mutations Causing Alzheimer Disease cause mis-processing of APP
-secretase -secretase
Aβ
AICD(?Signalling)
Extracellular
TM domain
Intracellular
APPmutations
APP
Citron et al. Nature Med. 3: 67-72, 1997
-secretase
Uptake, chaperoning, °radation of Aβ by neprilysin, IDE, others
FAD-causing mutations in APP are localized in/around the Aβ peptide domain.
CodonCodon MutationMutation PhenotypePhenotype EffectEffect670/671 Lys-Met/ FAD β-secretase cleavage
Asn-Leu692 Ala->Gly FAD Fibrillogenesis/toxicity693 Glu->Gln Haemorrhage Fibrillogenesis/toxicity
Glu->Gly Haemorrhage Fibrillogenesis/toxicity694 Asp->Asn Haemorrhage Fibrillogenesis/toxicity713 Ala->Thr FAD714 Thr->Ile FAD N-truncated Aβ42715 Val->Met FAD N-truncated Aβ42 716 Ile->Val FAD Aβ42717 Val->Ile/Phe FAD Aβ42
/Gly723 Leu->Pro FAD Aβ42
Extracellular
TM domain
Intracellular
APP
FAD-causing mutations in APP are alter the amount or the fibrillogenic potential of Aβ peptide
CodonCodon MutationMutation PhenotypePhenotype EffectEffect670/671 Lys-Met/ FAD β-secretase cleavage
Asn-Leu692 Ala->Gly FAD Fibrillogenesis/toxicity693 Glu->Gln Haemorrhage Fibrillogenesis/toxicity
Glu->Gly Haemorrhage Fibrillogenesis/toxicity694 Asp->Asn Haemorrhage Fibrillogenesis/toxicity713 Ala->Thr FAD714 Thr->Ile FAD N-truncated Aβ42715 Val->Met FAD N-truncated Aβ42 716 Ile->Val FAD Aβ42717 Val->Ile/Phe FAD Aβ42
/Gly723 Leu->Pro FAD Aβ42
Mutations Causing Alzheimer Disease cause mis-processing of APP
-secretase -secretase
A
AICD(?Signalling)
-secretase
Extracellular
TM domain
Intracellular
APPmutations
APP
Citron et al. Nature Med. 3: 67-72, 1997
PS1/PS2mutations
Uptake, chaperoning, °radation of Aβ by neprilysin, IDE, others
Naturally Occurring Mutations in Presenilins Alter APP Processing
Cytoplasm
Lumen
Membrane
• >100 missense/in-frame splicing mutations in PS1 scattered throughout PS1 molecule;
Sherrington et al. Nature 375: 754-760, 1995Rogaev et al Nature 376: 775-778, 1995Citron et al. Nature Med. 3: 67-72, 1997
• Predicted to encode homologous polytopic transmembrane proteins (PS1 and PS2).
• PS1 and PS2 mutations all alter Aβ production – increase Aβ42.
• > 12 mutations in PS2;
• Mutations in PS1 and PS2 often affect orthologous residues.
XD XGXGD
• Contain conserved aspartate residues in transmembrane domains (protease active site).
APH-1 NicastrinPEN-2
Cytoplasm
Lumen/Cell surface
Membrane
Presenilin Proteins Form a Complex With Nicastrin APH-1 and PEN-2 To Presenilin Proteins Form a Complex With Nicastrin APH-1 and PEN-2 To Cleave Amyloid Precursor Protein (APP) and generate neurotoxic ACleave Amyloid Precursor Protein (APP) and generate neurotoxic Aββ peptide. peptide.
Golgi/ER
Presenilin
A
-site
ε-site
AICD
Sherrington, Nature, 1995Rogaev, Nature, 1995Katayama, Nature Cell Biol, 1999Yu, Nature, 2000Chen, Nature Cell Biol, 2002Sisodia, Nature Neurosci, 2002Pardossi-Piquard Neuron, 2005
D D
Similar presenilin-dependent intramembranous cleavages for:
•Notch•Delta•p75•LRP1•SorLA•Others...
_AlzheimerDisease
Presenilin Mutations Cause Alzheimer Disease by altering γ-secretase cleavage of APP
-secretase -secretase-42
A
AICD(?Signalling)
-secretase
Extracellular
TM domain
Intracellular
APPmutations
APP
Citron et al. Nature Med. 3: 67-72, 1997
PS1/PS2mutations
Uptake, chaperoning, °radation of Aβ by neprilysin, IDE, others
Apolipoprotein E and Alzheimer’s Disease
• APOE has 3 variants: APOE has 3 variants: 2, 2, 3, 3, 4;4;
• APOE APOE 2 increased frequency in normal elderly, 2 increased frequency in normal elderly, reduced frequency in AD;reduced frequency in AD;
• APOE APOE 4 associated with Sporadic/familial AD 4 associated with Sporadic/familial AD (dose-dependent relationship with age of onset);(dose-dependent relationship with age of onset);
• APOE APOE 4 association not specific to AD, and not 4 association not specific to AD, and not all APOE all APOE 4 carriers will succumb to disease.4 carriers will succumb to disease.
• APOE APOE εε4 appears to 4 appears to block removal of Ablock removal of Aββ via LRP via LRP receptors, causing accumulation of Areceptors, causing accumulation of Aββ..
Mutations Causing Alzheimer Disease cause mis-processing of APP
-secretase -secretase
A
AICD(?Signalling)
↓ Uptake, chaperoning, & degradation of Aβ
-secretase
Extracellular
TM domain
Intracellular
APOE 4PS1/PS2mutations
APPmutations
Aaggregatesinto neurotoxicprotofibrils
A accumulates
APP
X
Citron et al. Nature Med. 3: 67-72, 1997
What’s the evidence for this linear pathway?
Enhancer and suppressor interactions amongst genes causing Alzheimer Disease
St George-Hyslop et al Science 263:536-537, 1994Pastor, P. et al. Ann Neurol 54, 163-9 (2003)
Gene interactions in human patients with AD:Gene interactions in human patients with AD:– APP717 mutation + APOE 4 allele = earlier onset (enhancerenhancer);
– APP717 mutation + APOE 2 allele = delayed onset (suppressorsuppressor);
– PS1E280A + APOE ε4 = earlier disease (enhancerenhancer)
– PS2N141V + APOE ε4 = earlier disease (enhancerenhancer) .
Gene interactions In animal modelsGene interactions In animal models– APP717 mutation + PS10/0 = no disease (suppressorsuppressor);
– APP717 mutation + PS1mutations = enhanced disease (enhancerenhancer).
Suppressor
APP genotype (AA= APP717)APOE Genotype
AA717717/WT
AA717717/WT
AA717717/WTAA717717/WT
WT/WTε2/ε3 ε3/ε3
ε4/ε3ε4/ε3εε2/2/ε3
Elderly (>65yrs old) asymptomatic carrier of APPV717I
mutation
APPV717I + APOE ε2 carrier eventually developed AD, but at >2 SD beyond mean age-of-onset.
Enhancer and suppressor interactions amongst genes causing Alzheimer Disease
St George-Hyslop et al Science 263:536-537, 1994Pastor, P. et al. Ann Neurol 54, 163-9 (2003)
Gene interactions in human patients with AD:Gene interactions in human patients with AD:– APP717 mutation + APOE 4 allele = earlier onset (enhancerenhancer);
– APP717 mutation + APOE 2 allele = delayed onset (suppressorsuppressor);
– PS1E280A + APOE ε4 = earlier disease (enhancerenhancer)
– PS2N141V + APOE ε4 = earlier disease (enhancerenhancer) .
Gene interactions In animal modelsGene interactions In animal models– APP717 mutation + PS10/0 = no disease (suppressorsuppressor);
– APP717 mutation + PS1mutations = enhanced disease (enhancerenhancer).
Enhancer effect of cross-breeding mutant PS1 and mutant APP mice
APP x PS1 mice - 2 months
PS1 mice - 2 months
APP mice – 2 months
Enhancer and suppressor interactions amongst genes causing Alzheimer Disease
• Confirms that the known AD genes really do act in the same biochemical pathway affecting APP processing.
St George-Hyslop et al Science 263:536-537, 1994Pastor, P. et al. Ann Neurol 54, 163-9 (2003)
What are the other genes?
General Paradigms for Gene Discovery
CASE : CONTROL ASSOCIATIONLINKAGE BASED
•Difficult to collect families•Expensive•Relatively few assumptions•Robust directly observable results
•Easy to collect sporadic cases•Cheap, quick•Easy to mess up•Requires assumption that cases and controls are from same founder population..
What are the other AD genes?Case:Control> 100 candidate genes reported to be associated with AD;
Generally had poor track-record of replication (NB: one or two ‘independent replications’ in the face of many non-replications = non-replication);
Family linkage-based methodConfirmed localization of an AD-gene to broad region of chromosome 10 containing several hundred genes (the specific gene remains to be found);
Confirmed localization of an AD-gene to broad region of chromosome 12 containing several hundred genes (the specific gene remains to be found)
What is the role for themicrotubule associated protein Tau
and neurofibrillary tangles?
Fronto-temporal dementia:Fronto-temporal dementia:molecular geneticsmolecular genetics
• Mutations in Tau gene on chromosome 17q in ~10-40% of FTD cases;
• Mutations disturb binding of tau protein to microtubules, causing accumulation of free unbound tau;
• Free unbound tau aggregates into fibrils and these then coalesce into paired helical filaments as the neurofibrillary tangle;
• The tau fibrils then injure cells (but mechanism is unclear).
Conclusions to Be Drawn From the Discovery of Pathogenic Mutations in Tau in FTD
• Disturbed tau/microtubule homeostasis, Disturbed tau/microtubule homeostasis, regardless of cause, is toxic to neuronsregardless of cause, is toxic to neurons
A accumulation initiates a biochemical cascade leading to neuronal death
Cause:Cause:(eg genedefect)
AA peptide peptideaccumulationaccumulation
NeuronalNeuronalinjuryinjury
Altered TauAltered Taumetabolismmetabolism
Neuronal Neuronal dysfunctiondysfunctionand deathand death
DementiaDementia
How is this knowledge applied for patients?
• Adjunctive Diagnostics
• Therapeutic Targets
Prediction of future risk for AD?
• Testing and genetic counselling feasible for: – Highly penetrant forms, with – Clear patterns of inheritance, and – Relatively predictable age-of-onset:
• PS1• APP• Tau
• Testing and genetic counselling not presently feasible/useful for: – Incompletely penetrant forms with variable age-of-onset:
• PS2• APOE• Putative genes on chromosomes 10, 12 etc
– NB: Advent of future therapies may make even fuzzy-risk data from such genes useful
Can Genetics Predict Conversion From MCI To AD?
• Intuitive expectation: – Carrier of AD risk allele with MCI would be more likely to convert to
AD.
• Actual data available only for ApoE
• ApoE ε4 predictive:– Petersen et al, JAMA 274: 538,1995 – Bartrez-Faz et al, JAGS 49: 485, 2001
• ApoE ε4 not predictive:– Marquis et al, Arch. Neurol. 59: 601, 2002– Tierney et al, Neurol. 46: 149, 1996.
Prediction of therapeutic response
• Theoretically reasonable; • Remains to be validated.
ADADStep 1 Step 4Step 3Step 2
Gene 1 Environment factor 1Gene 2
Rx 1 Rx 2
Using A accumulation pathways as a target for therapies
Cause:Cause:(eg genedefect)
AA peptide peptideaccumulationaccumulation
NeuronalNeuronalinjuryinjury
Altered TauAltered Taumetabolismmetabolism
Neuronal Neuronal dysfunctiondysfunctionand deathand death
DementiaDementia
Exploiting Knowledge Gained to Create New Diagnostics and Therapeutics
Cause:Cause:(eg genedefect)
NeuronalNeuronalinjuryinjury
Altered TauAltered Taumetabolismmetabolism
Neuronal Neuronal dysfunctiondysfunctionand deathand death
DementiaDementia
AA peptide peptideaccumulationaccumulationX
•Anti-A antibodies to remove A•Block enzymes;•Block aggregation.
Janus et al Nature. 408: 979-982, 2000,McLaurin et al, Nature submitted, 2004
How can the amyloid cascade be blocked?
-secretase -secretase
A
AICD(?Signalling)
Pharma:Pharma:
Aaggregatesinto neurotoxicprotofibrils
A accumulates
Citron et al. Nature Med. 3: 67-72, 1997
APP
Cell membrane
Uptake, chaperone, ordegradation (by neprilysin).
Vaccine:toxic
Pharma
X
Conclusions:
• All known genes causing AD modulate APP and Aβ processing;
• Neurodegeneration from mutations in tau prove that tau accumulation is also a toxic event (regardless of whether caused by mutation in tau or due to Aβ accumulation)
• Knowledge of pathway will provide targets for disease-modifying therapies.
Acknowledgements
Canadian Institutes of Health ResearchHoward Hughes Medical InstituteAlzheimer Society of Ontario,Canadian Genetic Diseases Network
S. ArawakaF. ChenL. Farrer,P. FraserYJ. GuH. HasegawaM. IkedaT. KatayamaT. KawaraiG. Levesque M. NishimuraA. PetitE. RogaevaN. SanjoP. St George-HyslopD. Westaway
A. Bruni, F. CheclerJF Foncin, G. Marcon, M. Mortilla, A. Orlacchio,E. PaitelS. Piacentini, L. Pinessi,I. Rainero, S. Sorbi, R. Tupler, G. Vaula
CONTACT INFORMATION
• Analysis of familial cases:P. St George-Hyslop, University of Torontotel: [email protected]
• Animal models (transgenic mice etc):David [email protected]
• Reagents (clones, cell lines, antibodies, etc)P. St George-Hyslop, University of [email protected]
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