Supplementary Material

Supplementary Figure 1 Emission scan of BT_3296 incubated with starch-derived sugars. The concentration of BT_3296 (navy blue) was 12 μM, where as the concentration of maltose (orange), maltotriose (green) and maltoheptaose (purple) was 5 mM. The concentration of pullulan (cyan) was ~10 mg/ml, -βcyclodextrin (red) was 10 mM and amylopectin (light blue) was ~0.2% (w/v).

Supplementary Figure 2 Predicted Model of BT_3296. Predicted model of BT_3296 with the modeled substrate of β-cyclodextrin (A). Tyr335 is believed to be one of the main residues involved in binding the substrate β-cyclodextrin. Overall sequence based homology for BT_3296 in comparison to identified SusD-homologs sequences in Consurf (B).

Supplementary Figure 3 Crystal structures of BT_3299 and BT_0339. Arrangement of molecules in the asymmetric unit of BT_3299 (A). Alignment of structures BT_3299 (slate blue) and BT_0339 (cyan green) (B). Arrangement of molecules in the asymmetric unit of BT_0339 (C).

Supplementary Table 1 Polysaccharide Utilization Locus Component

Supplementary Table 2 Primers for BT_3296. Sequence of primers used in the amplification of the BT_3296 gene for the plasmid pET29a. Restriction sites BamHI and XhoI were inserted for the forward primer BT_3296-F1.1 and the reverse primer BT_3296-R1.1 respectively as indicated by underlining.