VereChipTM

Khilda Afifah23214316

Mengintegraskan Biochips dengan silicon heaters, temperature sensors and

miniaturized 25μl volume allow the PCR module

Product

VereFlu untuk mendeteksi Influenza A: H1, H3, H5, H7, H9 with subtype ID’s of H1N1, A/H1N1-

2009, H3N2, H5N1 and H9N2 and Influenza B.

VereMTB to detect, differentiate and identify: 10 different mycobacterium strains with special

emphasis on Mycobacterium tuberculosis Complex (MTBC) and its Resistance to Rifampicin

and/or Isoniazid from Pulmonary Clinical Specimens or Cultivated Samples (MDR-TB)

VereTrop to detect, differentiate and identify Multiple Tropical Diseases

VereTheat to detect, differentiate and identify Anthrax, Small Pox, Plague and Tularemia

VereFoodborne to detect, differentiate and identify multiple foodborne Pathogens in one test

Verevet to detect, differentiate and identify multiple Avian Pathogens in one test

VereMERS to detect and identifies Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Spesifikasi VereFoodborneTM

Detects multiple foodborne pathogens

Multiple Probes (with duplicates) for Bacillus spp., Campylobacter jejuni / lari /

coli, Clostridium perfringens, Cronobacter sakazakii, Listeria spp., Salmonella

spp., Escherichia coli, Shigella spp., Staphylococcus aureus, Vibrio cholera, Vibrio

parahaemolyticus, stx1A gene, stx2A gene, etc

Process controls on each chip:

• PCR: Positive and Negative Controls

• Hybridization: Orientation and Hybridization Probes

Limit of Detection: 100 copies of extracted genomic DNA

Sample Types**: meat, fish, vegetables, milk, water, processed foods, stool or

vomit sample

Every chip is bar-code

Integrasi VereFoodborneTM

VerePLEXTM Biosystem menggabungkan biologi

molekuler, mikrofluida dan mikroelektronika untuk

membawa masa depan diagnostik. VerePLEXTM Biosystem

termasuk komponen-komponen berikut:

Temperature Control System (5 random access

modules)

Optical Reader

Biosystem Software

Barcode Reader

Feature & Cara Kerja Multiplex amplification reactions

Multiple probes per target ensures reliable detection of subtypes in every test

Small sample volume requirement

Fast and programmable temperature ramp rate

PCR yield is comparable to standard thermal cyclers

40% faster than conventional thermal cyclers

Proprietary microfluidic interface: contact surfaces are biocompatible and do not inhibit the PCR reaction

Short time required for fluidic operations

Technology & Manufaktur Chip silikon yang inovatif menggabungkan Micro-Electro Mechanical Systems

(MEMS) dengan mikrofluida yang memungkinkan integrasi multiplexing amplifikasi

nukleat asam dan deteksi dalam satu perangkat

The integrated silicon heaters, temperature sensors and miniaturized 25μl volume

allow the PCR module to perform the rapid heating and cooling cycles required for

highly reliable, end-point PCR.

The microfluidic integration of the PCR and hybridization modules ensures that

system operation does not require highly-trained personnel, and greatly simplifies

the typical lab procedures.

Diproduksi dengan kontrol room kelas 100 dan quality control 6 sigma metodology

dan termasuk on-the fly spotting with error detection

http://vereduslabs.com/technology/technologyverechip-technology/

6 sigma metodology (DMAIC)

Define adalah fase menentukan

masalah, menetapkan persyaratan-

persyaratan pelanggan, mengetahui

CTQ (Critical to Quality)

Measure adalah fase mengukur tingkat

kecacatan pelanggan (Y)

Analyze adalah fase menganalisis

faktor-faktor penyebab masalah/cacat

(X)

Improve adalah fase meningkatkan

proses (X) dan menghilangkan faktor-

faktor penyebab cacat

Control adalah fase mengontrol kinerja

proses (X) dan menjamin cacat tidak

muncul

Proses Fabrikasi

A variety of structures was made using this process flow: microchannels, mixers, microreactors, micropillar filters,

capillary pumps and valves, droplet generators (for digital droplet polymerase chain reaction, or PCR), mergers,

and splitters

a) A silicon wafer (grey color) is covered with an oxide layer (green color).

b) Then, a nitride layer (blue color) is added.

c) An etch is performed to remove nitride and oxide, and silicon is excavated to form the coarse structures.

d) The exposed silicon surface is oxidized. This oxide will serve as protection during the definition of fine structures.

e) The nitride is removed and the fine structures are etched

f) the oxide is removed

g) the silicon is bonded to Pyrex

h) A backside etch is performed to open fluidic connections.http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001

Contoh Teknologi dengan PCR

http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001

Pertama, sampel darah diambil

Kedua, pompa mendorong darah dan reagen ke

modul pra-amplifikasi. Modul ini terdiri dari mixer,

reaktor termal untuk PCR, dan filter.

Ketiga, dalam modul analisis, DNA dimurnikan

dicampur dengan reagen, allele-specific PCR

dilakukan sehingga hanya fragmen DNA dengan

SNP diperkuat, dan produk sampingan dari

amplifikasi terdeteksi dengan detektor

elektrokimia

Kelebihan

Memudahkan untuk mengontrol epidemi

pada makanan

Tes yang dipilih generik dan spesifik dari

patogen makanan yang di uji

Lab on Chip yang dirancang portable dan

dapat dioperasikan dengan mudah tanpa

membutuhkan tenaga ahli

Probe dapat di upgrade yang

memungkinkan dapat mendeteksi patogen

baru hasil dari mutasi

Perbandingan dengan Metode Konfensional (bacteriological Analytical Manual)

Terimakasih Banyak