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Varvara Trachana Curriculum Vitae

Ι. Personal Information

Place of Birth: Athens, Greece

Work address: Office A.308, Department of Biology, Faculty of Medicine, Panepistimiou 3, Biopolis, 41500, Larisa

Place of residence: Larisa

E-mail 1: vtrachana@med.uth.gr

E-mail 2: vtrachana@gmail.com

Tel: +30 2410 685624

FAX: +30 2410 685549

Google scholar: https://scholar.google.gr/citations?user=KAPzA4oAAAAJ&hl=el

ResearchGate: https://www.researchgate.net/profile/Varvara_Trachana

Scopus: https://www.scopus.com/authid/detail.uri?authorId=6508087861

II. Current Position July 2014 - : Assistant Professor of Cell Biology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Biopolis, 41500 Larissa, Greece ΙΙΙ. Education

• 1997: Bachelor, School of Biology, Faculty of Science, Aristotle University of

Thessaloniki (AUTH), Thessaloniki, Greece.

• 2003: PhD in Biological Chemistry, Faculty of Medicine, School of Health Science,

AUTH, Thessaloniki, Greece.

IV. Languages:

Greek (native), English, Spanish

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V. Research Posts

• 1994-1996: Diploma Thesis, Laboratory of Animal Physiology School of Biology,

Faculty of Science, AUTH, Thessaloniki, Greece.

• Feb 1996-May 1996: Erasmus scholar, Consell Superior I' Investigacions

Cientifiques (CSIC) /Centre D' Investigacio i Desenvolupament/ Department of

Molecular Biology, Barcelona, Spain.

• Aug 1997-Aug 1998: Research Assistant, National Peripheral Center of

Histocompatibility, Department of Immunology, Hippokration Hospital, Thessaloniki,

Greece.

• Nov 1999-Apr 2001: Research Assistant, Research Project Grant, entitled "Study of

the early markers of diabetic complications and development of diagnostic kits",

Laboratory of Biological Chemistry, Faculty of Medicine, School of Health Science

AUTH, Thessaloniki, Greece.

• Aug 1998-Mar 2003: PhD Candidate: “Study of the biological role of the

phosphorylation of laminin”, Laboratory of Biological Chemistry, Faculty of Medicine,

School of Health Science AUTH, Thessaloniki, Greece.

• Nov 2003- Dec 2007: Postdoctoral researcher, Department of Immunology and

Oncology (DIO), National Centre of Biotechnology (CNB) /CSIC, Madrid, Spain.

• Jan 2008-Jul 2011: Research Associate, Molecular and Cellular Ageing Programme,

Institute of Biological research and Biotechnology (IBRB), National Hellenic Research

Foundation (NHRF), Athens, Greece

• March 2011: Elected Assistant Professor in Cell Biology, Faculty of Medicine,

School of Health Sciences, University of Thessaly, Larisa, Greece.

• January 2013-October 2014: Research Associate, Research Project StemAge,

School of Biology, Faculty of Science, AUTH, Thessaloniki, Greece

• July 2014-present: Assistant Professor in Cell Biology, Faculty of Medicine, School

of Health Sciences, University of Thessaly, Larisa, Greece.

VI. Research Projects Grants • Greece: 1)PENED 1999-2001: “Study of the early markers of diabetic complications and development of diagnostic kits”.

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2) ProteomΑge 2008-2011/ Coordination Action within the 6th Framework Programme) “Functional analysis of evolutionarily conserved mechanisms of ageing on advanced proteome analysis” 3) StemAge 2013-2016/ 600.000 euros, entitled: “Revealing strategies with antioxidant/antiageing properties in human stem cells” (Partner) 4) 1st prize award/ 4.000 euros, from the University of Thessaly for the research study

entitled “Study of the effects of oxidative stress on the function of centrosomes in human

mesenchymal stem cells” (V. Trachana, principal investigator) (Academic Advisor)

5) 2018- ‘Erevno-Dimiourgo-Kainotomo” Project/ 999.999,98 euros, Entitled “ Induced Pluripotent Stem Cell in the Cell Therapy of Osteoarthritis” (Deputy Academic Advisor) 6) Support for Young Scientists- 2019-today: Entitled “The role of SIRT-1 in osteoarthritis: functional interaction(s) between lipid metabolism-autophagy and study of the implicated epigenetic mechanisms” (Deputy Academic Advisor).

7) IKY scholarship for the phD candidate A. Goutas

• Spain: 2003-2008 1) GEN2001-4856-C13-01: Spanish Ministry of Education

2) Grant PI051965: Spanish Ministry of Health

VII. Peer Reviewed Journal Publications (in parenthesis 5-year journal impact factor (IF) 1. Kaloyianni M, Trachana V, Boukla A “Regulation of Cation Transport Pathways and Glycolytic Enzyme Activity by Alterations in Red cell Volume” Cell Biochem. Funct. 17, 75-88 (1999) (IF 1.5). 2. Koliakos G, Kouzi-Koliakos K, Triantos A, Trachana V, Kavoukopoulos E, Gaitatzi M, Dimitriadou A “Laminin-1 phosphorylation by protein kinase A: Effect on self assembly and heparin binding” J Biochem Mol Biol 33(5), 370-378 (2000) (IF 1.773). 3. Koliakos G, Chatzivasiliou V, Dimopoulos Th, Trachana V, Paschalidou K, Galiamoutsas V, Triantos A, Chitas G, Dimopoulos A, Vlatsas G “The significance of PSA/IGF-1 ratio in differentiating benign prostate hyperplasia from prostate cancer” Disease Markers 16, 143-146 (2000) (IF 2.445). 4. Koliakos G, Trachana V, Gaitatzi M, Dimitriadou A “ Phosphorylation of laminin-1 by Protein kinase C” Mol Cells 11(2), 179-185 (2001) (IF 1.998). 5. Koliakos G, Papachristou F, Papadopoulou M, Trachana V, Gaitatzi M, Sotiriou I “Electrophoretic Analysis of Urinary Proteins in Diabetic Adolescents” J Clin Labor Anal 15, 178-183 (2001) (IF 0.975). 6. Kouzi-Koliakos K, Sapountzi Z, Papageorgiou A, Trachana V, Kotsinou S, Koliakos G “Anti-idiotypic antibodies carrying the “internal image” of peptide YIGSR inhibit spontaneous metastasis of Lewis Lung carcinoma in mice” In vivo 16, 511-518 (2002) (IF 1.091). 7. Trachana V, Christoforides E, Kouzi-Koliakos K, Koliakos G “Laminin-1 is phosphorylated by ecto-protein kinases of monocytes” Int J Biochem Cell Biol 37, 478-492 (2005) (IF 4.543).

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8. Konstantinidis D, Koliakos G, Vafia K, Liakos P, Bantekas C, Trachana V, Kaloyianni M. “Inhibition of the Na+-H+ exchanger isoform-1 and the extracellular signal-regulated kinase induces apoptosis: a time course of events” Cell Physiol Biochem 18 (4-5), 211-22 (2006) (IF 3.459). 9. Trachana V*, van Wely KHM, Guerrero-Alonso A, Fütterer A, Martinez-A C. “Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability” Proc Natl Acad Sci U S A (PNAS). 20 (104, 8), 2691-2696 (2007) (* corresponding author) (IF 10.228) 10. Kostidou E, Trachana V, Topouridou K, Paletas K, Tsapas A, Kaloyianni M, Koliakos G.” Monocyte attachment to laminin in diabetes mellitus: The role of ATP” Cell Adh Migr.3(2), 155-9 (2009) (IF 2.3). 11. Prieto I, Kouznetsova A, Fütterer A, Trachana V, Leonardo E, Alonso Guerrero A, Cano Gamero M, Pacios-Bras C, Leh H, Buckle M, Garcia-Gallo M, Kremer L, Serrano A, Roncal F, Albar JP, Barbero JL, Martínez-A C, van Wely KH “Synaptonemal complex assembly and H3K4Me3 demethylation determine DIDO3 localization in meiosis” Chromosoma 118(5):617-32 (2009) (IF 4.202). 12. Alonso Guerrero A, Cano Gomero M, Trachana V, Fütterer A, Pacios-Bras C, Panadero N, Cigudosa J, Martinez-A C, van Wely KH “Centromere-localized breaks indicate the generation of DNA damage by the mitotic spindle” Proc Natl Acad Sci U S A (PNAS) 107(9):4159-64 (2010) (IF 10.228). 13. Sisoula C, Trachana V*, Patterson C, Gonos ES “CHIP-dependent degradation of p53 is essential for normal transition to cellular senescence” Free Radical Biology and Medicine (doi:10.1016/j.freeradbiomed.2010.10.701) (2011) (* corresponding author) (IF 6.326). 14. Tsolou A¶, Nelson G¶, Trachana V, Chondrogianni N, Saretzki G, von Zglinicki T, Gonos ES “The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult” (¶: equal contribution) IUBMB Life 64 (5):432-42. (2012) (IF 3.377) 15. Villares R, Gutiérrez J, Fütterer A, Trachana V, Gutiérrez del Burgo F Martínez-A C “Dido mutations trigger perinatal death and generate brain abnormalities and behavioral alterations in surviving adult mice” Proc Natl Acad Sci U S A (PNAS) 112(15):4803-8. (2015) (IF 10.228). 16. Trachana V*, Petrakis S, Fotiadis Z, Siska E, Balis V, Gonos E, Kaloyianni M, Koliakos G “Human mesenchymal stem cells with enhanced telomerase activity acquire resistance against oxidative stress-induced genomic damage” Cytotherapy 19(7): 808-820 (2017) (* corresponding author) (IF 3.62). 17. Trachana V¶, Ntoumou E¶, Anastasopoulou L, Tsezou A “Studying microRNAs in osteoarthritis: Critical overview of different analytical approaches” (¶: equal contribution). Mechanisms of Ageing and Development (MAD) 171: 15-23 (2018). (IF 3.7). 18. Goutas A, Syrrou C, Papathanasiou I, Tsezou A, Trachana V* “The autophagic response to oxidative stress in osteoarthritic chondrocytes is deregulated” Free Radical Biology and Medicine 126:122-132 (2018) (* corresponding author) (IF 6.326). 19. Papathanasiou I, Trachana V, Mourmoura E, Tsezou A “DNA methylation regulates miR-140-5p and miR-146a expression in osteoarthritis” Life Sciences (2019). doi: 10.1016/j.lfs.2019.05.018. [Epub ahead of print] (IF 3.2). 20. Goutas A, Papathanasiou Ι, Mourmoura Ε, Tsesmelis Κ, Tsezou Α, Trachana V* “Overexpression and spatiotemporal regulation of caveolin-1 forms part of the oxidative stress response of chondrocytes” BBA (under review-second round or revision) (* corresponding author) (IF 5.5). 21. Papathanasiou Ι, Trachana V, Balis C, Mourmoura Ε, Tsezou Α “The synergistic function of miR-140-5p and miR-146a on TLR4mediated cytokine secretion in osteoarthritic chondrocytes” BBRC (under review) (IF 2.5)

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B. Invited review (peer reviewed) 22. Trachana V, Mourmoura E, Papathanasiou I, Tsezou A “Understanding the role of chondrocytes in osteoarthritis: utilizing proteomics” Expert Review of Proteomics 16(3):201-213 (2018) (IF 3.489). C. Scientometris -Peer Reviewed Journal Publications 23. Taylor M, Perakakis P, Trachana V “The siege of science” Ethics in Science and Environmental Politics 8 (1): 17-40 (2008). 24. Perakakis P, Taylor M, Mazza M, Trachana V “Natural Selection of Academic Papers” Scientometrics 85 (2): 553-559 (2010) (IF 2.2) 25. Perakakis P, Taylor M, Mazza M, Trachana V “Understanding the role of open peer review and dynamic academic articles” Scientometrics 88 (2): 669-673 (2011) (IF 2.2) 26. Taylor M, Perakakis P, Trachana V, Gialis S “Rankings are the sorcerer’s new apprentice” Ethics in Science and Environmental Politics doi 10.3354/esep00146 (2014). 27.Perakakis P, Taylor M, Trachana V “The roads to open access” UNESCO World Social Sciences Report pages 307-309 (2010). D. Publications in international peer reviewed conference proceedings 1. Trachana V “Ectoprotein kinase activity and attachment to laminin of monocytes derived

from healthy and diabetic individuals” FEBS JOURNAL 268: 189-143 Suppl. 1 (2001). 2. Koliakos G, Paletas K, Kostidou E, Trachana V, Tsapas A “Modulation of monocyte

attachment to laminin by ATP” Atherosclerosis Suppl 6 (1), 17-17 (2005). 3. Koliakos GG, Kostidou E, Trachana V, Tsapas A, Kaloyianni M, Paletas K “Monocyte

attachment to laminin in diabetes mellitus” Diabetes 54, A193-A194 Suppl 1 (2005). 4. Sisoula C, Trachana V, Kapeta S, Gonos ES “CHIP ligase plays a central role in p53

regulation during senescence” FEBS JOURNAL 276:252-252 Suppl. 1 (2009). 5. M Kaloyianni, A Pouikli, L Kyrka, S Petrakis, G Koliakos, Trachana V “Telomerase

overexpression in human mesenchymal stem cells offers protection against oxidative DNA damage accumulation” Cytotherapy 17 (6), S34-S35 (2015)

6. Goutas A, Tsezou A, Trachana V “The chondroprotective function of autophagy against acute exogenous oxidative insult in osteoarthritic chondrocytes is impaired” Free radical Biology and Medicine 120 Supl. 1 (2018).

E. Main Presentations in International Conferences/ Meetings/Workshops 1. Trachana V, Koliakos G, Triantos A, Kavoukopolos E, Dimitriadou A. XVIIth Meeting of

the Federation of the European Connective Tissue Societies (FECTS). “Laminin1-nidogen1 complex is a substrate of protein kinases, Patras, Greece 2000 (poster)

2. Trachana V, Koliakos G, Paschalidou K, Dimitriadou A. 18th International Congress of Biochemistry and Molecular Biology. “Ectoprotein kinase on monocytes and lymphocytes phosphorylates laminin1-nidogen1 complex” Birmingham, UK 2000 (oral)

3. Trachana V, Koliakos G, Kaltezioti V, Labadiari V, Dimitriadis G, Raptis S, Tsilibary E. 27ο Συνέδριο της Ένωσης Ευρωπαϊκών Εταιριών Βιοχηµείας (FEBS) “Ectoprotein kinase

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activity and attachment to laminin of monocytes derived from healthy and diabetic individuals” Lisbon, Portugal 2001(oral)

4. Trachana V, Koliakos G, Kaltezioti V, Mitrou P, Labadiari V, Dimitriadis G, Raptis S, Tsilibary E 40th Jubelee Session of Varna University of Medicine, 2nd Bulgarian- Greek Meeting. “Ectoprotein kinase activity of monocytes and its possible influence by diabetic complications” Varna, Bulgaria 2001(oral)

5. Trachana V, van Wely K, Alonso Guerrero A, Fütterer A, Martinez-A C Conférences Jacques-Monod “Molecular Machines in Cell Division” (Les Machines Moleculaires de la division cellulaire) “Dido3: a novel centrosome-associated protein and its role in cell cycle progression” Roscoff (Brittany), France 2005 (poster)

6. Trachana V, Fütterer A and Martínez-A C EMBO Practical course on microinjection and detection of probes in cells “Dido mutations link centrosomal aberrations and genomic instability to alterations in stress responses”, EMBL Heidelberg, Germany 2007 (poster)

7. Sisoula C, Trachana V, Kapeta S, Gonos E. 34th FEBS Congress “Life’s molecular interactions”. “CHIP ligase plays a central role in p53 regulation during senescence” Prague, Czech republic, 2009 (poster)

8. Trachana V, García García MΙ, Hoeijmakers JHJ, Marninez-A C and Gonos E. 3rd Annual Scientific Meeting of Proteomage (Coordination Action within the 6th Framework Programme), “The implication of the proteasome in the early onset of ageing: Possible novel roles of an old player” Seefeld, Austria, 2008 (oral)

9. Trachana V, Hoeijmakers JHJ, Gonos ES MarkAge/LinkAge (Coordination Actions within the 6th Framework Programme) Joint Summer School “The implication of the Ubiquitin Proteasome System in the early onset of ageing” Fréjus, France 2009 (oral)

10. Tsolou Α, Trachana V, Gonos ES 13th Congress of the International Association of Biomedical Gerontology “Aging, cancer and age-related diseases; common mechanisms?” “ Role of 19S proteasome subunits in DNA damage and stress-induced premature senescence” Québec, Canada, 2009 (poster)

11. Sisoula C, Trachana V, Kapeta S and Gonos ES Annual meeting of LinkAge “CHIP overrides Mdm2-mediated degradation of p53 during replicative senescence” Brussels, Belgium. 2009 (poster)

12. Sisoula C, Trachana V, Kapeta S and Gonos ES FEBS Practical Course on Protein Interaction Modules “CHIP ligase plays a central role in p53 regulation during senescence” Split, Croatia. 2009 (poster)

13. Trachana V, Marteijn J, Hoeijmakers JHJ, Gonos E 3rd Swedish-Hellenic, Life Science Research Conference/ National Hellenic Research Foundation - Örebro University “The implication of the Ubiquitin–Proteasome system in the early onset of ageing” Athens, Greece, 2010 (best poster award)

14. Trachana V “Why We Age” (A European road map to molecular biogerontology-Cooperacion action whithin the 7th Framework Programme) meeting “Proteasome function in early onset of ageing and age-related diseases” Vouliagmeni, Greece 2010 (oral)

15. Chondrogianni N., Georgila K., Trachana V., Valianatos G., Kourtis N., Tavernarakis N. and Gonos ES “Proteasome activation extends cellular and organismal lifespan” Final Annual Scientific Meeting of the European program PROTEOMAGE, Budapest, Hungary, 2011 (oral)

16. Balis, V, Ntogka, A, Fotiadis, Z, Petrakis, S, Koliakos, G, Kaloyianni, M, Trachana, V* “Telomerase overexpression in human mesenchymal stem cells offers protection against oxidative DNA damage accumulation”, International Society for Stem Cell Research (ISSCR) Annual Meeting, Stockhom, Sweden, 2015 (oral)

17. E. Ntoumou, M. Braoudaki, G. Lambrou N. Stefanou, I. Papathanasiou, M. Poulou, L. Anastasopoulou, K. Malizos, V. Trachana, M. Tzetis, A. Tsezou “Circulating microRNA

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signature as novel biomarker for osteoarthritis development” European Human Genetics Conference, Copenhagen, Denmark, 2017 (poster)

18. Goutas A, Tsezou A, Trachana V* “The chondroprotective function of autophagy against acute exogenous oxidative insult in osteoarthritic chondrocytes is impaired”, Society for Free Radical Research – International Biennial Meeting, Lisbon, Portugal, 2018 (poster)

19. Goutas A, Fotiadis Z, Telligianidou A, Nikolopoulou A, Trachana V* “Gadd45a is involved in oxidative stress response and senescence onset in human mesenchymal stem cells” Society for Free Radical Research – Europe Meeting, Ferrara, Italy, 2019 (travel award) (poster and selected oral)

(*corresponding author)

VIII. Summary of Scientific work Publications 25 (+2 under

review)

Puplication in conference procceedings 6

1st author 5

2nd author 7

Corresponding author 6

Citations (Google scholar) 400

h index (Google scholar) 11

i10 index (Google scholar) 11

Total impact factor 89,368 (97,468)

Mean impact factor 3,9 (4,23)

Academic advisor of research project 5

Oral Presetantions in Greek conferences 11

Oral Presetantions in International Conferences 10

IX. Collaborations Α) In Greece:

1) Laboratory of Animal Physiology: Department of Biology, Aristotle University of Thessaloniki/Prof. M. Kalogianni

2) Laboratory of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, Prof. G. Koliakos

3) Laboratory of Molecular and Cellular Senescence, Institute of Chemical Biology, National Hellenic Research Foundation / E.S. Gonos

Β) Abroad

1) Department of Immunology and Oncology (DIO), National Centre of Biotechnology (CNB) /CSIC, Madrid, Spain /: Prof. C. Martizez-A

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2) Department of Immunology and Oncology (DIO), National Centre of Biotechnology (CNB) /CSIC, Madrid, Spain /:Dr Karel van Welly

3) Microscopy Core Facility of the Institut de Microbiologie de la Méditerranée, Μασσαλία, Γαλλία/ Head of the facility, Dr. Artemis Kosta

Χ. Teaching Ø Undergraduate courses, Faculty of Medicine, School of Health Sciences,

University of Thessaly, Larisa, Greece

• Biology I (Cell Biology) 1st year undergraduate course) (since July 2014) • Biology II (Molecular Biology) 1st year undergraduate course) (since July 2014) • Principles of Human Genetics (3rd year undergraduate course) (since July 2014)

Ø Postgraduate (master) courses, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larisa, Greece

• Master program “Human Genetics”, Greece ) (since July 2014).

Research supervision

Postgraduate theses (masters, PhDs) Principal Supervisor • Supervisor of 9 master theses, Faculty of Medicine, University of Thessaly (up to 2019) • Supervisor of 1 PhD thesis in progress (2017-) Associate Supervisor • Co-supervisor of 30 master theses, Faculty of Medicine, University of Thessaly (up to

2019) • Co-supervisor of 1 PhD theses, Faculty of Medicine, University of Thessaly (up to 2019) PhD Examiner • PhD examiner in 2 PhD theses, Faculty of Medicine, University of Thessaly (up to 2018) ΧI. Scholarships/Awards • Erasmus scholarship February 1996-August 1996.

• Funded to attend the Forum of Young Scientist (FEBS)/Protein Structure and Function

Trafficking and Signalling, (Oeiras, Portugal, 2001)

• Funded to attend 27th FEBS/PABMB meeting (Lisbon, Portugal, 2001).

• “Honorary member of the Academic Community of Varna University of Medicine” title

awarded for presenting a plenary paper at the Jubilee Session “New technologies in

medicine: Philosophical and Ethical problems” (Varna, Bulgaria, 2001)

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• Best poster award 3rd Hellenic-Swedish Life Science Conference (Athens 2010).

• 1st prize award/ from the University of Thessaly for the research study as principal

investigator

ΧII. Reviewer 1. Mechanism of Aging and Development (MAD) 2. Medicine® 3. Webmed Central Plus (Aging expert) 4. Ethics in Science and Environmental Politics (ESEP) 5. Reports in Cancer and Treatment 6. Current Chemical Biology 7. Journal of Stem Cell and Transplantation Biology 8. Cell Transplantation 9. Current Stem Cell Research & Therapy 10. Clinical Interventions in Aging 11. Journal of Stem Cell Research and Medicine 12. Cells 13. Journal of Clinical Medicine 14. Heliyon 15. International Journal of Environmental Research and Public Health 16. Aging Research Reviews ΧIII. Editorial boards member 1. Member of WebmedCentral Plus Editorial Board on Aging 2. Journal of Stem Cell and Transplantation Biology 3. Journal of Cellular Toxicology and Cell Biology 4. Cell biology and Cell Metabolism XIV. Member of Scientific Societies

1. Member of the Hellenic Society of Biochemistry and Molecular Biology 2. Member of Hellenic Society of Bioscientists 3. Member of the Hellenic Association of Medical Geneticists 4. Member of the Society of Free Radical Research (SFRR 5. Member of the Society for Redox Biology and Medicine