Upright MicroscopeSeries+2CE-MQVH-4...Nikon has developed a new photoactivation unit for upright...

Upright Microscope ECLIPSE Ni

Upright Microscope

▌System expandability •Nikon’sproprietarystratumstructureenablesefficientsystemconstruction. •Thenumerousaccessoriescanbecustomcombineddependinguponapplication.

▌Optical performance •CFIPlanApochromatλ objectiveswithchromaticaberrationcorrectionandhigh transmissionthroughoutabroadrangeofwavelengths •ObjectiveswithimprovedtransmissioninnearIRwavelengthrangeformultiphoton excitationimaging

▌Design •3Dergodesigncombinesfunctionalitywithsophistication.

▌Operability •Ni-E:Motorizedmodelwithautomaticchangeofobservationconditionsand adjustmentofmicroscopeaccessories. •Ni-U:Manualmodelwithsomemotorizedoptions. •Mostmicroscopecontrolscanbeoperatedwitheasy-to-reachbuttonsonthefrontoftheNi-E.

TheNiseries,theultimateinuprightbiologicalmicroscopeevolution,supportsbiosciencestudieswithenhancedbasicperformanceandflexiblesystemexpandability.ThenewlydevelopedCFIPlanApochromat λ (lambda)seriesobjectivesarethekeytotheseries’opticalperformance.NanoCrystalCoat,withitsultralowrefractiveindex,isemployedforthefirsttimeinmicroscopeobjectives,providingbrighter,high-resolutionandhigh-contrastmicroscopyimages.Nikon’sproprietarystratumstructure,usedandhighlyacclaimedforinvertedresearchmicroscopes,isnowincorporatedinanuprightmicroscope,allowingacombinationofcomponentstobeinstalled.Applicationsusinglaserandfluorescentproteins,suchasKaedeandPA-GFP,arepossiblewiththeadditionofatwo-tieredfluorescentunitandaphotoactivationunit.TheNiseriestranscendstheconceptofconventionaluprightmicroscopesandexpandsthepossibilitiesofadvancedresearchinfieldssuchasbioscienceandmedicine.

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See the evolution

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The Ni series microscopes meet all research demandsThesemicroscopeshavethreefeatures:expandabilityofthesystemduetothestratumstructurethatenablesopticalpathsintwotiers;superioropticalperformance,asrepresentedbythePlanApochromatλ seriesobjectives;andhigh-speedmotorizedaccessories.TheNiseriesisdesignedtomeettheneedsofalladvancedbioscienceandmedicalresearch.

Cell biology

Pathology

Neuroscience

Developmental biology

Immunology

Genetics

Microbiology

Drug discovery

Cancer research

Microscopyimagescourtesyof:(Left)TorstenWittmann(Center)MichaelW.Davidson(Right)JenniferWatersandAdrianSalic

Ni-E

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Multiphoton imaging (Ni-E)Multiphotonmicroscopyinwhichlongexcitationwavelengthsareusedtoallowless-invasiveimagingofeverdeeperareasofcellsisgainingpopularity.ThedesignoftheNi-Emodelisoptimizedformultiphotonimaginginbothopticalandmechanicalsystemstomeetthedemandsoftoday.

In vivo imaging

Two-photon imaging

Photo- activation

▌Objective dedicated to multiphoton imagingCFI75Apochromat25xWMPobjectivefeatureschromaticaberrationcorrectionoverabroadwavelengthrangefrom405to950nm,highNA(numericalaperture)(1.10)andlongerworkingdistance(2.0mm),andcompatibilitywithwaterimmersionandwaterdipping.

▌High-sensitivity multiphoton detector (NDD)NewlydevelopedepiscopicNDD(non-descanneddetector)unitincorporatesadetectorthatefficientlysensesweaksignalsfromdeepareasoflivespecimens.IncombinationwithadiascopicNDDunit,transmittedsignalscanbealsodetected.

▌Retrofittable focusing nosepiece mechanismMicroscopescanbemodifiedbyswitchingthefocusingstageandfocusingnosepiece,enablingfixed-stageconfigurationtomeetdemandsofexperimentssuchasinvivoimaging.

MountedepiscopicNDDunitanddiascopicNDDunit

Ｆ．ＳＴＯＰＡ．ＳＴＯＰ

ＥＸ．ＡＤＪ．

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Expandable system broadens application possibilities

Ni-E

Multi-color fluorescent imaging (Ni-E) Demandformulti-colorfluorescentimagingthatusesnewlydevelopedfluorescentproteinsandfluorescentreagentsisconstantlyincreasing.Nikonmeetssuchneedswithdiversefunctionsandopticaltechnologies.

Multi-color fluorescent observation

FISH

Signal conversion

▌High-speed motorized componentsThehigherspeedsofthemotorizedexcitationandbarrierfilterwheelandthemotorizedshutterenablequickwavelengthchanges,reducingphotobleachingofthespecimen.Itcanbeoperatedviaeasilyaccessiblecontrolbuttons,increasingoperationefficiency.

▌CFI Plan Apochromatλ series objectivesTransmissionandchromaticaberrationcorrectionhavebeenimprovedthroughoutthewiderangeofvisibletonearIRwavelengths,allowinguseofvariousfluorescentreagents.Theyprovidebright,high-contrast,highS/N(signal-to-noise)ratiomulti-colorfluorescenceimageswithalmostnofocusshiftwhenusedwithanywavelength.

Mountedmotorizedexcitation/barrierfilterwheel



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Photoactivation imaging (Ni-E/Ni-U) Researchintothereactionsandchangesofstimulatedcellshasbecomepopularinrecentyears.Nikonhasdevelopedanewphotoactivationunitforuprightmicroscopes,afirstinmicroscopy.

Photo- activation

FRAP

▌Flexible stratum structureThisstructureallowstwo-layermountingofaphotoactivationunitwithanepi-fluorescenceattachment.

▌Objectives for long-wavelength laserWithCFIPlanApochromat λ objectives,chromaticaberrationhasbeencorrectedupto850nmandtransmissionimprovedinthelongwavelengthrange,increasingaccuracyandefficiencyoflaserexcitationattargetsite.

Ni-E Ni-U

Mountedphotoactivationunit



0

Complies with FDA performance standard for laser products except for deviations pursuant to Laser Notice No.50,dated June 24,2007.

IEC/EN60825-1:2007

Total Power 500mW MAX.CW 400～700nm

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Simultaneous multichannel imaging (Ni-E/Ni-U)TheNi’sbackportandthequadroculartiltingtubeallowtheusertoacquiresimultaneous,two-channelimagesonseparatecameras.ThisfeatureisinvaluableforapplicationssuchasFRET.

FRET

Ratio imaging

▌Simultaneous imaging with two camerasTheNi’sflexiblestratumstructureenablesincorporationofabackcameraportunit,allowingsimultaneousimageacquisitionoftwodifferentwavelengthswithtwodifferentcamerasmountedonthebackportunitandthequadroculartiltingtube.Thisenablesthecaptureofhigh-resolutionimagesintheentireframeforeachwavelengthwithoutdividingtheCCDchip.Theuseofindividualcamerasforacquisitionallowstheusertotailoracquisitionparametersforeachchannelindependently,allowingacquisitionofhigh-sensitivityFRETimages.

Ni-E Ni-U

Mountedbackportunit

Ni-E

Multiphoton imaging (Ni-E)Multiphotonmicroscopyinwhichlongexcitationwavelengthsareusedtoallowless-invasiveimagingofeverdeeperareasofcellsisgainingpopularity.ThedesignoftheNi-Emodelisoptimizedformultiphotonimaginginbothopticalandmechanicalsystemstomeetthedemandsoftoday.

In vivo imaging

Two-photon imaging

Photo activation

▌Objective dedicated to multiphoton imagingCFI75Apochromat25xWMPobjectivefeatureschromaticaberrationcorrectionoverabroadwavelengthrangefrom405to950nm,highNA(numericalaperture)(1.10)andlongerworkingdistance(2.0mm),andcompatibilitywithwaterimmersionandwaterdipping.

▌High-sensitivity multiphoton detector (NDD)NewlydevelopedepiscopicNDD(non-descanneddetector)unitincorporatesadetectorthatefficientlysensesweaksignalsfromdeepareasoflivespecimens.IncombinationwithadiascopicNDDunit,transmittedsignalscanbealsodetected.

▌Retrofittable focusing nosepiece mechanismMicroscopescanbemodifiedbyswitchingthefocusingstageandfocusingnosepiece,enablingfixed-stageconfigurationtomeetdemandsofexperimentssuchasinvivoimaging.

MountedepiscopicNDDunitanddiascopicNDDunit



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Versatile microscopes meet all demands

Ni-U (manual model)・ Ergonomictubeandstagehandleheightadjustmentmechanismallowcomfortableviewingpositions.・ Stratumstructureandsturdydesignimproveexpandability.・Motorizednosepiece,motorizedepi-fluorescencecubeturret,motorizedshuttercanbeutilized.

Ni-E (motorized model)・ High-precisionmotorizedfocusing・ Broadrangeofmotorizedaccessoriesthatcanbeusedincombination.・ Observationconditionscanbechangedatasimplepushofabutton.・ Stratumstructureandsturdydesignimproveexpandability.・ 3Dergodesignbuttonswithimprovedoperabilityarelocatedclosetogetherforspeedyoperations.・Microscopesettingsinusecanbeverifiedonthedisplay.・ Optimizedformultiphotonexcitationimaging・ Twofocusingmechanismoptions:focusingstageandfocusingnosepiece

Manual and motorized models Tomeetdiverseuserdemandsforoperability,systemexpandabilityandmotorizedcontrol,NikonprovidestwoNiseriesmodels.TheNi-U,whichhascompatibilitywithsomemotorizedaccessories,isthemanualmodelsuitableforhigh-qualityimageobservationanddigitalimaging.TheNi-Eisafullymotorizedmodelthatisefficientforexperimentsrequiringcomprehensivecontrolofvariousdevices,suchasphotoactivationunitsandconfocalsystems.

Ni-E provides fully motorized operations

Automatic adjustment with objective changeoverCondenser,apertureandfielddiaphragm,andNDfilterareautomaticallysettotheoptimalpositionduringobjectivechangeover.Inaddition,stageXYZtravelamountperhandlerotationandparfocaldistancedeviationcorrectionareautomaticallyadjusted.Microscopesettingscanalsobemanuallyadjusted.

Change of observation conditionsSelectedobservationconditionscanbedesignatedtoindividualbuttons,enablingchangestobemadeatthepushofabutton.Thisisparticularlyconvenientwhenreproducingspecificobservationconditions.

High-precision motorized focusingHigh-precisionZ-focusincorporatedbytheNi-EprovidesaccurateZ-positioninformationrequiredforusewithconfocallasermicroscopes.Individualcoarseandfinefocusknobsprovideenhancedeaseofoperation.

Ni-Econfiguredwithamotorizedepi-fluorescencecubeturretandmotorizeduniversalcondenser

Ni-Uconfiguredwithanergonomicbinoculartube

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Lens Lens

Reflected light Reflected lightIncident light Incident light

Nano Crystal CoatThisanti-reflectivecoatingthatconsistsofnanometer-sizeparticlesisbasedonsemiconductormanufacturingtechnologyandisalsousedforNikoncameralenses.Thecoarsestructurewithparticlesarrangedinaspongyconstructionwithuniformspacesbetweenthemenablesextremelylowrefractiveindices.

Supreme optical performance Asalightmicroscopemanufacturer,Nikonhascultivatedhightechnicalcapabilitiesandconfidence.Withitsadvancedtechnologiesextendingfromopticalglassproductiontolensdesign,fabrication,coatingandprocessing,Nikonprovidesunsurpassedopticalperformance.

・ CFI Plan Apochromatλ seriesWithremarkablyhighNA,greatlyimprovedtransmissioninthelongwavelengthrangethankstoNikonproprietaryNanoCrystalCoat,andchromaticaberrationcorrectionover435to850nm,theseobjectivesareideallysuitednotonlyforbrightfieldandDICobservationsbutalsoforfluorescentobservations.Theselensesallowacquisitionofbrightandclearimagesatanywavelengthfornear-IRimagingandmulti-colorfluorescenceimaging.Becausebrightimagescanbecapturedevenwithaweakexcitationlight,damagetoaspecimenisminimized.

・ Water dipping objective lensesWithalongworkingdistanceandhighNA,theseobjectivesprovideexcellenttransmissioninnearIRwavelengthrange.Theaxialchromaticaberrationof40xand60xobjectiveshasbeencorrectedtoupto850nm,allowinghigh-resolutionimagesofminutestructuresinthickspecimensduringIR-DICobservation.The25xWMPand100xobjectivesfeaturehighNA(1.1)andalongworkingdistance(2.0mm).WithchromaticaberrationcorrectedintheIRregion,theseobjectivesareidealformultiphotonexcitationobservation.Inaddition,byemployingamechanismtocompensateforthechangesinsphericalaberrationthatoccuratdifferenttemperaturesanddepthsofobservationpoints,clearimagesofareasdeepwithinathickspecimencanbecaptured.

Uniformly bright illuminationThe“fly-eye”lensisideallysuitedtodiascopicilluminationopticalsystems.Uniformandbrightilluminationuptotheviewfieldperipheryisprovidedatanymagnification.

High-performance objective lens

Epi-fluorescenceobservation

Phasecontrastobservation

DIC(DifferentialInterferenceContrast)observation

Brightfieldobservation

Excellent image acquisition with all observation methods

Fluorescence filter cube

Lens

Camera

Light source

Light absorbing material

NDStray light

Technologies supporting the Ni series

Conventionalmulti-layercoatingWithoutNanoCrystalCoat WithNanoCrystalCoat

NanoCrystalCoat

Fluorescence noise eliminationNikon’sproprietarynoiseterminatormechanismisemployedintheepi-fluorescencecubeturretandfiltercubes.TheS/Nratiohasbeendramaticallyimprovedbythoroughlyeliminatingstraylightinthefiltercubes,allowingimagesofweakfluorescentsignalstobecapturedwithhighcontrastandbrightness.

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Digital imagingTheNiseriescanbecontrolledinconjunctionwithaDigitalSightseriesdigitalcamera,facilitatingeffortlessdigitalimaging.Imagescanbecapturedwithadedicatedbuttononthemicroscopebody,thetouchpanelonthecameracontrolunit,orthesoftwareGUIonaPC,dependingonyourpreference.

Ultimate ease, speed and clarity in imaging

Digital Sight series digital cameras

Awidearrayofmodelswithhighsensitivity,highresolution,highspeedsandfaithfulcolorreproductionareavailabletomeetallresearchneedsandpurposes.

Imaging Software NIS-Elements

NIS-Elementsseamlesslyintegratescameras,peripheraldevices,andtheNi,servingasapowerfulyeteasy-to-useinterfaceforcompleximagingexperiments.Powerfultoolsforquickprocessing,measurementandacquireddatamanagementprovideaone-stepsolutionforacquisitionandanalysis.

・ Image capture buttonImagescanbeacquiredbysimplypressingtheimagecapturebuttonlocatedonthemicroscopebase.

・ High-sensitivity cooled monochrome camera head DS-Qi1ThenewCCDdrivecircuitreducesreadoutnoise.Thecoolingmechanismreducesheat-inducednoise,allowingthecaptureofweakfluorescencesignals.Furthermore,thereisahighframerateofupto48fpsandahighquantitativelinearitywithin2%.

・ Ultrahigh-resolution cooled color camera head DS-Ri1Providesthe12.7-megapixelequivalent,2200TVlinehighdefinition.Smoothliveimagedisplayatmax.32fpsmakesfocusingeasy.Superiorcolor　reproductioncapabilitiesallowrecordingofaccuratespecimencolors.Thecoolingmechanismreducesheat-inducednoiseinfluorescentimages.

・ High-definition cooled color camera head DS-Fi1cEquippedwitha5-magapixelCCD.Thecoolingmechanismreducesheat-inducednoise,makingitidealforfluorescentimageacquisition.

・ High-definition color camera head DS-Fi2Equippedwitha5-magapixelCCD.Displaysliveimagesatmax.21fps.Minutestructurescanbecapturedinbrightfield,phasecontrastandDICobservations.

・ Stand-alone control unit DS-L3Stand-alonecontrolunitDS-L3allowstheDigitalSightseriesdigitalcameratobesetupandoperatedwithoutaPCconnection.ItalsoenablesimageacquisitionfromaPCthroughanetwork.TheDS-L3isfirmwarebased,andthereforewillnotcompromiseyourfacility’snetwork.

・Optimalimagingparametersforeachobservationmethodcanbeselected usingiconsandsimplemeasurementscanbetaken.・Camera,motorizedmicroscopefunctionsandperipheraldevicescanbe comprehensivelycontrolled.・Microscopestatusdata,suchasobjectivelens,fluorescentfiltercubeand condenserinformation,isautomaticallysavedalongwiththe capturedimagewhenamotorizedorintelligentunitisattached.・Operationscanbecontrolledwithamouseoratouchpaneloperatedby touchorstyluspen.・Requirediconscanbeselectedandlaidoutasdesired.

Imagecapturebutton

Camera/microscopecontrol

L3ASSIGNMODEGUIforscanareasetting

MicroscopecontrolScenemodeicons

Histogram

Imagestitching(largeimage)

Mergechannels

・CombinationofNi-Eandmotorizedstageenableslarge-imageacquisition.

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CombiningtheNi-E’shigh-precisionZ-focusmechanismwithaconfocalscannerallowshigh-resolution,high-S/N-ratioimagingof3Dstructuresoforgansandcells.TheNi-Ecanbeconfiguredwitheitherafocusingstageorafocusingnosepiece,cateringtospecificimagingrequirements.Nikonoffersawiderangeofconfocalsystemsthatcanaccommodatevariousneeds.

・ Multiphoton confocal microscope A1 MP+/A1R MP+

A1MP+enableshigh-resolutionmultiphotonimaging(upto4096x4096pixels).Inaddition,A1RMP+iscapableofhigh-speedmultiphotonimagingofupto420fps.Thehigh-performancedetectorsenabledeepimagingoflivingspecimenswithhighsensitivity.TheNDDalsoenableshigh-speed,accurateunmixingofGFPandYFP.

・ Confocal microscope A1+/A1R+

A1+incorporatesahigh-definition(upto4096x4096pixels)non-resonantscanner.A1R+alsoincorporatesahigh-speed(upto420fps)resonantscanner,inadditiontothenon-resonantscanner,enablingtruesimultaneousphotoactivationandimaging.

・ Confocal microscope C2+

C2+featuresacompactdesignandhighfunctionality.TheC2+allowsuserstoacquiresimultaneous3-channelanddiascopicDICimagesandprovidespowerfulimagingmodalities,suchaslarge-imagestitching.

・ Spectral imaging confocal microscope A1si+/A1Rsi+/ C2si+

Byincorporatingaspectraldetector,awidewavelengthspectrumof320nmcanbeacquiredwithasinglescan.Closelyoverlappingspectracanbeunmixedcleanlywithminimalcrosstalk.

Privetleafsection(fluorescencefromFastGreen,Safranin,CrystalViolet,andOrangeG)(capturedwithA1)

High-resolution confocal imaging systems

Ni-EconfiguredwithC2+

RK13rabbitkidneyepithelialcellstablytransfectedwiththecalciumbiosensorYellowCameleon3.6.ThecellwasexposedtoionomycintoraiseintracellularcalciumandinduceFRET.ThecolorchangeindicateschangesinthelevelofFRET.Time-lapseimagesweretakenwiththeA1R(resonantscanner).Samplecourtesyof:Dr.MikeDavidson,NationalHighMagneticFieldLaboratory,FloridaStateUniversity

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Cryosectionofamousekidneylabeledwithphalloidin(actin;red),wheatgermagglutinin(green),andDAPI(DNA;blue)(capturedwithA1)

Mousefibroblastlabeledwithtubulinantibodies(blue)andDAPI(DNA;magenta)(capturedwithA1)

Adultmouseisletstainedwithanti-proinsulin,anti-betacateninandanti-KDELR(ERmarker).Photoscourtesyof:Drs.DivaJonatan,MattKofronandJamesWells,CincinnatiChildren’sHospitalMedicalCenter

E10.5mouseembryowholemountstainedwithanti-Pdx1(green),anti-Nkx2.1antibodies(blue)andantiE-cadherintorevealthedevelopingpancreaticandlungprimordia.Photoscourtesyof:Drs.DivaJonatan,MattKofronandJamesWells,CincinnatiChildren’sHospitalMedicalCenter

BovinebrainmicrovascularendothelialcellslabeledwithMitoTracker(mitochondria,yellow),phalloidin(actin,blue)andHoechst(DNA,magenta).(capturedwithA1)

Privetleafsection(fluorescencefromFastGreen,Safranin,CrystalViolet,andOrangeG)(capturedwithA1)

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Dimensional diagram

130

60205

437

604

320

389



A.S

JAPAN

FN-S2N

0

Complies with FDA performance standard for laser products except for deviations pursuant to Laser Notice No.50,dated June 24,2007.

IEC/EN60825-1:2007

Total Power 500mW MAX.CW 400～700nm

320 383

205

340

60488

130

A

λ



320 383

205

340

60

130

501

Ni-E (for use with focusing nosepiece)ConfiguredwithanNiphotoactivationunit,two-tieredmotorizedepi-fluorescencecubeturretandmotorizedquadroculartiltingtube

Ni-UConfiguredwithamotorizedepi-fluorescencecubeturretandquadroculartiltingtube

Ni-UConfiguredwithanergonomicbinoculartubeandDSCport

Unit: mm

Wide array of accessories finely segmented by function

Motorizedchangeoverofopticalpathsispossible.Eyepieceinclinationcanbeadjustedfrom15°to35°.

Effectiveforapplicationsthatrequirehighlyaccuratepositioning,suchasphotoactivationimagingandFISH.

s.

High-speedmotorizedchangeoverofcondensermodulesforbrightfield,phasecontrast,DICandsimpledarkfieldobservationsispossible.

Laserlightphotoactivationandepiscopicilluminationarepossible.Boththephotoactivationunitandtheconfocalsystemcanbeusedwithasinglelasersource.

NoiseterminatorprovideshighS/Nratio.Sixfiltercubescanbeinstalled.Eitheranepi-fluorescenceattachmentoraphotoactivationunitcanbeattached.

Adigitalcameracanbemountedonthecameraport.Amotorized0.6x-2.0xzoomopticalsystemisincorporated.

MakescontrolofmotorizedXYstagepossible.

Barrierfilterpositions(7positions—using25mmfilters)canbechangedatahighspeedof0.2sec.betweenadjacentpositions.

Enablessimultaneousacquisitionofimageswithtwodifferentwavelengthsusingtwocameras.Dedicatedcubesareoptional.

High-speedshuttercontrolispossible.Theshuttercanbeattachedtodiascopicandepiscopicilluminationsystems.

Brightnessisautomaticallyoptimizedwiththechangeoverofthemotorizednosepiece.Motorizedadjustmentofdesiredbrightnessisalsopossible.

Inadditiontomotorizedmicroscopeoperation,XYZcontrolofstageispossiblewithsimilaroperationaleaseasthatofanactualmicroscope.

Excitationfilterpositions(8positions—using25mmfilters)canbechangedatahighspeedof0.15sec.betweenadjacentpositions.

Objectivemagnificationisautomaticallysavedalongwiththecapturedimage.Built-inprism/analyzerplateslot.

Motorizedoperationofnosepiece,epi-fluorescencecubeturretandshutterispossible.

Motorized quadrocular tilting tube (Ni-E)

Motorized XY stage (Ni-E)

Motorized universal condenser Dry (Ni-E)

Photoactivation unit (Ni-E, Ni-U)

Motorized epi-fluorescence cube turret (Ni-E, Ni-U)

Motorized DSC zooming port (Ni-E)

Joystick for motorized stage (Ni-E)

Motorized barrier filter wheel (Ni-E)

Back port unit (Ni-E, Ni-U)

Motorized shutter (Ni-E, Ni-U)

Motorized ND filter (Ni-E)

Ergo controller (Ni-E)

Motorized excitation filter wheel (Ni-E)

Motorized sextuple DIC nosepiece (Ni-E, Ni-U)

Simple remote control pad (Ni-U)

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System diagram: Ni-E focusing stage type

Dａｒｋ Fｉｅｌｄ CｏｎｄｅｎｓｅｒOｉｌ 1.43 - 1.20

JAPAN

Dａｒｋ Fｉｅｌｄ CｏｎｄｅｎｓｅｒDｒｙ 0.95 - 0.80

JAPAN

0.20.40.60.81.01.21.4

Aｃｈｒ-Aｐｌ N.A=1.4

JAPAN

D-C Oi l

JAPAN

JAPAN

Coarse

Fine

Ex Fine

XY SpeedConstant Speed

Z Speed

Coarse

Fine

Ex Fine

+Y

-Y

-X +X

POWER

MIN. MAX.

POWER

I 0SUPER HIGH PRESSUREMERCURY LAMPPOWER SUPPLY

RUN TIME

IGNITION

POWERLAMPREADY

REPLACE BULBBEFORE THESPECIFIED TIME

O I

MADE IN JAPAN

LAMP HOURS

PUSH ON

POWERLIGHT

XENON POWER SUPPLY XPS-100

0

D-FB



ｘ４／２

ｘ４／

A1

*6

*1

*9

*2

*7

*7

*5*5

*4

*2

*1

*1

*1 Double layer is maximum possible.*2 When used with double layer, “J” connection should be lower layer and “I” connection should be upper layer. Single “J” layer is not possible.*3 These cubes can only be used with “I” connection.*4 When both “I” and “J” are connected.*5 Cannot be used with double layer status.*6 Cannot be used with double layer status.*7 Should be attached when used with both Ni-PAU and Confocal.*8 C2 can only be used with single layer status.*9 Can be used in combination with NI-FLEI, by attaching either of the two on top of the other. Use the unit with the double layer structure (cannot be used with single layer status).

C-HGFIF15/30 HG Fiber

UN2 Transformer 100W

Halogen Lamp Socket 100W

HMX Lamphouse

C-FCL Epi-Fl Collector Lens

Quartz Epi-Fl Collector Lens

Xenon Power Supply 75W

C-SHG1 Power Supply for HG100W

Xe Lamp Socket 75W

Xe Lamphouse HMX-4


Hg Lamphouse HMX-4B


Mercury Lamp Socket S 100W BL

Hg Lamphouse HMX-3B

C-HGFI/HGFIE HG Precentered Fiber Illuminator Intensilight

C-LHGFI HG Lamp

C-HGFIB HG 100W Adapter R

C-IA Analyzer Tube for First-order Red Compensation

C-ISA Analyzer Tube for Simple Polarization

C-ER Eyelevel Riser

Y-IDP Double Port

Y-IDT Drawing Tube

D-ES Slider for Reflected Illumination

NI-PAU Ni Photoactivation Unit

TI-SHAEP-I Motorized Shutter Adapter for Inverted Epi-FL

NI-EXW-E Motorized Excitation Filter Wheel

NI-SH-E Motorized Shutter

NI-SHAEP-U Motorized Shutter Adapter for Upright Epi-FL

NI-SHCS Motorized Shutter Cable Short

D-FB Excitation Balancer

NI-FLEI EPI-Fluorescence Attachment

NI-BPU Back Port Unit

NI-BAW-E Motorized Barrier Filter Wheel

TI-A DIC Analyzer Cube

C-FL Epi-fl Filter Cube

NI-FLT6-E Motorized Epi-fluorescence Cube Turret

NI-FLT6-I Intelligent Epi-fluorescence Cube Turret

NI-FLT6 Epi-fluorescence Cube Turret

NI-FA FL/DIC Analyzer

NI-AST Analyzer Slider Stopper

DS-L3 DS Camera Control Unit

USB CableNI-CTLA Control Box A

NI-ERG NI Ergo Controller

NI-SJ Joystick for Motorized Stage

D-DP DIC Rotatable Polarizer

C-TP Polarizer for First-order Red Compensation

C-SP Polarizer for Simple Polarization

NI-LH Precentered Lamphouse

NIE-CB Connector Box

NI-SHADI Motorized Shutter Adapter for Dia-Illumination



NI-ND-E Motorized ND Filter

ECLIPSE Ni-E

D-C DF Darkfield Module

D-C PH-1 PH ModuleD-C PH-2 PH ModuleD-C PH-3 PH Module

D-C DIC N1 DRY DIC ModuleD-C DIC N2 DRY DIC ModuleD-C DIC NR DRY DIC Module

D-C 2-4X Auxiliary Lens for N2 Position

NI-CALN1 2-4X Auxiliary Lens for N1 Position

NI-CUD Universal Condenser Dry

D-C DIC Module Oil

D-CUO DIC Condenser Oil

C-C Achromat Swing-out Condenser 1-100x

X LWD Condenser

C-C Abbe Condenser NA 0.9

C-C Achromat Condenser NA 0.9

C-C Achromat/Aplanat Condenser

Darkfield Condenser for X/Y (Dry)

C-C Slide Achromat Condenser 2-100x

Darkfield Condenser for X/Y (Oil)

NI-CUD-E Motorized Universal Condenser Dry

NI-CH Condenser Holder

NI-SSR Substage for Rotatable/Motorized Stage

NIE-CSRR2 Right Handle Rotatable Ceramic-coated Stage with Holder

NI-S-E Motorized XY Stage

CFI UW 10x M

CFI UW10x

C-CT Centering Telescope

CFI 15xCFI 10x MCFI 10xCFI 12.5x

NI-RPS DSC Support Column

NI-RPSAB DSC Adapter B

NI-RPSAA DSC Adapter A

NI-RPZ DSC Zooming Port for Quadrocular Tube

NI-RPZ-E Motorized DSC Zooming Port for Quadrocular Tube

NI-TT QuadrocularTilting Tube

NI-TT-E Motorized Quadrocular Tilting Tube

C-TAQ Tube Adapter for Quadrocular Tube

DIC Slider

D-LP Lambda Plate

C-AS Analyzer Slider for First-order Red Compensation

D-SA Analyzer Slider for Simple Polarization

D-DA Analyzer Slider for DIC CFI60

Objective Lens

NI-N7-E Motorized Septuple Nosepiece

NI-ND6-E Motorized DIC Sextuple Nosepiece

NI-N7-I Intelligent Septuple Nosepiece

NI-ND6-I Intelligent DIC Sextuple Nosepiece

D-ND6 DIC Sextuple Nosepiece

C-NA Sextuple Nosepiece with Analyzer Slot

L-DIC DIC Slider

L-NU5 Universal Quintuple Nosepiece ESD

L-NBD5 BD Quintuple Nosepiece ESD

L-DIHC DIC Slider HC

CFI60 Objective Lens

CFI LU Plan Fluor EPI Objective Lens

CFI LU Plan Fluor BD Objective Lens

NIE-CAM Contact ArmNI-SAM Standard Arm

NI-DRS DSC Rotation Stopper

Y-TV 0.55 TV Tube

Y-TV TV Tube

V-T Photo Adapter

C-mountTV AdapterVM4x

C-mount TV Adapter VM2.5x

Relay Lens 1x

C-mount TV Adapter

TV ZoomLens

Relay Lens 1x

C-mount Adapter 0.55x

C-mount Adapter 0.7xC-mount

TV Adapter A

C-mount TV Adapter 0.35x, 0.45x, 0.6x

C-mount Zooming Adapter

ENG-mount Zooming Adapter

ENG-mount TV Adapter

ENG-mount TV Adapter 0.45x, 0.6xProjection

Lens PLI 2x, PLI 2.5x, PLI 4x, PLI 5x

C-mount CameraENG-mount CameraFX-III Series Photomicrographic System

C-TEP2 DSC Port for Ergonomic Binocular Tube

C-TE2 Ergonomic Binocular Tube

C-TT Trinocular Tube T

C-TF Trinocular Tube F

C-TB Binocular Tube

*8 C-T T-C Trinocular Tube

C2 Adapter

A1 Adapter

C2

Achromat Swing-out Condenser 2-100x

C-N Sextuple Nosepiece

LU Nosepiece Adapter

LV-LH50PC 12V50W Precentered Lamphouse

M

FFE

FE

F

KKK’

OOK

B

BB

ba

a’b’ b’

a’

ba

OR

F

FF E

P

R

R

M

M

M

K

S

Q

R

O

K

N

K’

K’

K’

Q

KK’ QK

J

I

I

I

S

N

N’

N’

J

K

GG G

PP

EM K’

FE

FE

D-FLE Filter Cube for Reflected Illumination

C-FLF FRET CubeC-FLF Mirror CubeC-FL2 Blank Cube

D-FLD Filter Cube for Darkfield Illumination

*3LL

λ

2

3

4

2

3

4

λ

W

W T T

T

T

T

UV

T TU

U

VT TUV

V

22 23

JAPAN

JAPAN

Coarse

Fine

Ex Fine

XY SpeedConstant Speed

Z Speed

Coarse

Fine

Ex Fine

+Y

-Y

-X +X

D-FB



N 1 D r y

JAPAN

USB Cable


A1

*1 *2

*5

*5

*2

*1

*1

*1 Double layer is maximum possible.*2 When used with double layer, “J” connection should be lower layer and “I” connection should be upper layer. Single “J” layer is not possible.

*3 These cubes can only be used with “I” connection.*4 Cannot be used with double layer status.*5 Should be attached when used with both Ni-PAU and Confocal.*6 Can be used in combination with NI-FLEI, by attaching either of the two on top of the other. Use the unit with the double layer structure (cannot be used with single layer status).



C-LHGFI HG Lamp




TI-SHAEP-I Motorized Shutter Adapter for Inverted Epi-FL

NI-EXW-E Motorized Excitation Filter Wheel






NI-BAW-E Motorized Barrier Filter Wheel


A1-FN1-IR Analyzer Block IR








NI-CTLA Control Box A

NI-ERG NI Ergo Controller

NI-SJ Joystick for Motorized Stage

NIE-CB Connector Box







NIE-CAM Contact ArmNI-SAM Standard Arm


Y-TV 0.55 TV Tube

Y-TV TV Tube

V-T Photo Adapter



Relay Lens 1x

C-mount TV Adapter

TV ZoomLens

Relay Lens 1x



TV Adapter A









*4 C-TT Trinocular Tube T

*4 C-TF Trinocular Tube F

C-TB Binocular Tube

C2 Adapter

A1 Adapter

C2


NI-RPZ-E Motorized DSC Zooming Port for Quadrocular Tube


NI-TT-E Motorized Quadrocular Tilting Tube

*4

CFI UW 10x M

CFI UW10x

C-CT Centering Telescope

CFI 15xCFI 10x MCFI 10xCFI 12.5x


NI-SSF Substage for Focusing Nosepiece

D-C DIC N2 DRY DIC Module

D-C DIC N1 DRY DIC Module

FN-PT4X 4X Support Lens

FN-C LWD Condenser

NI-SH-D Dish Holder

NI-S-E Motorized XY Stage

NI-SA Motorized XY Stage Adapter

FN-3PS2 FN1 Standard Stage

NI-3PSA Adapter for FN1 Standard Stage


DIC SliderCFI75 Objective Lens

ObjectiveSlider

Objective Holder

DIC Slider

NI-FNA Focusing Nosepiece Sub Arm

NI-AMSL Arm Spacer 74mm

NI-AMSS Arm Spacer 49mm

NI-PT PolarizerTurret


NI-IRLH Precentered IR Lamphouse

NI-FIA Fiber Illumination Adapter


NI-ND-E Motorized ND Filter

ECLIPSE Ni-E

FN-IRP IR PloarizerFN-P Polarizer

Fiber Illuminator

M

O

F

FF

EF

K

KK’OKO

KR

P

M

S

Q

R

KK’

K’

K’

Q

KK’ QK

J

I I

S

N

N

J

K

G

G G

PP

E

M

R

M

M

K’

FE

FE

FF E

L

*6


I




*3L

System diagram: Ni-E focusing nosepiece type

24 25

System diagram: Ni-U

*10


I

JAPAN

JAPAN



Y-IDT Drawing Tube

Y-IDP Double Port

*7

*1

*1

*1

*8

*2

*2

*5

*5

*4

*8

*6*9 *6

*1 Double layer is maximum possible.Upper layer accepts only NI-FLT6 when double layer is used.

*2 When used with double layer, “J” connection should be lower layer and “I” connection should be upper layer. Single “J” layer is not possible.

*3 These cubes can only be used with “I” connection.*4 When both “I” and “J” are connected.*5 DS-L3 can be connected directly when Ni-CTLB is not used.*6 Cannot be used with double layer status.*7 Cannot be used with double layer status.*8 Should be attached when used with both Ni-PAU and Confocal.*9 C2 can only be used with single layer status.*10 Can be used in combination with NI-FLEI, by attaching either of the two on top of the other. Use the unit with the double layer structure (cannot be used with single layer status).




D-C DF Darkfield Module

D-C PH-1 PH ModuleD-C PH-2 PH ModuleD-C PH-3 PH Module

D-C DIC N1 DRY DIC ModuleD-C DIC N2 DRY DIC ModuleD-C DIC NR DRY DIC Module

D-C 2-4X Auxiliary Lens for N2 Position

NI-CALN1 2-4X Auxiliary Lens for N1 Position

NI-CUD Universal Condenser Dry

D-C DIC Module Oil

D-CUO DIC Condenser Oil

C-C Achromat Swing-out Condenser 1-100x

X LWD Condenser

C-C Abbe Condenser NA 0.9

C-C Achromat Condenser NA 0.9

C-C Achromat/Aplanat Condenser

Darkfield Condenser for X/Y (Dry)

C-C Slide Achromat Condenser 2-100x

Darkfield Condenser for X/Y (Oil)

C-C Phase Contrast Turret Condenser

CFI UW 10x M

CFI UW10x

C-CT Centering TelescopeCFI 15xCFI 10x MCFI 10xCFI 12.5x







NIU-CAM Contact ArmNI-SAM Standard Arm


Y-TV 0.55 TV Tube

Y-TV TV Tube

V-T Photo Adapter



Relay Lens 1x

C-mount TV Adapter

TV ZoomLens

Relay Lens 1x



TV Adapter A










C-TT Trinocular Tube T

C-TF Trinocular Tube F

C-TB Binocular Tube

C-T T-C Trinocular Tube

C2 Adapter

C2

UN2 Transformer 100W

Halogen Lamp Socket 100W

HMX Lamphouse


Quartz Epi-Fl Collector Lens

Xenon Power Supply 75W

C-SHG1 Power Supply for HG100W

Xe Lamp Socket 75W

Xe Lamphouse HMX-4


Hg Lamphouse HMX-4B


Mercury Lamp Socket S 100W BL

Hg Lamphouse HMX-3B


C-LHGFI HG Lamp




NI-SRCP Simple Remote Control PadNI-CTLB

Control Box B

C-CTC Camera Trigger Cable U3/L3

C-CTC Camera Trigger Cable U3/L3

TI-100WRC 100W Lamphouse Remote Cable

D-DP DIC Rotatable Polarizer

C-TP Polarizer for First-order Red Compensation

C-SP Polarizer for Simple Polarization


NI-SHCL Motorized Shutter Cable Long


ECLIPSE Ni-U

NI-CH Condenser Holder

NI-SSR Substage for Rotatable/Motorized Stage

NIU-CSRR2 Right Handle Rotatable Ceramic-coated Stage with Holder

C-H1L Specimen Holder 1LC-H2L Specimen

Holder 2L

NI-SS Substage

C-CSR1S Right Handle Ceramic-coated Stage with 1S Holder

C-SR2S Right Handle Stage with 2S Holder

C-CSR Right Handle Ceramic-coated Stage





NI-SHCL Motorized Shutter Cable Long





C-ER Eyelevel Riser

C-ISA Analyzer Tube for Simple Polarization

C-IA Analyzer Tube for First-order Red Compensation

Y-THPL LED Pointer Unit for Teaching Head

Y-IM Magnification Module

Y-THF Teaching Unit Face to Face

Y-THS Teaching Unit Side by Side Unit B

TH-AC2 AC Adapter Y-THM Main Teaching Unit

Y-THR Teaching Unit Side by Side A

Y-THSP Support for Side by Side

Achromat Swing-out Condenser 2-100x

DIC Slider

D-LP Lambda Plate

C-AS Analyzer Slider for First-order Red Compensation

D-SA Analyzer Slider for Simple Polarization

D-DA Analyzer Slider for DIC CFI60

Objective Lens

L-DIC DIC Slider

L-NU5 Universal Quintuple Nosepiece ESD

L-NBD5 BD Quintuple Nosepiece ESD

L-DIHC DIC Slider HC


LU Nosepiece Adapter

CFI LU Plan Fluor EPI Objective Lens

CFI LU Plan Fluor BD Objective Lens



LNI-N7-E Motorized Septuple Nosepiece

NI-ND6-E Motorized DIC Sextuple Nosepiece

NI-N7-I Intelligent Septuple Nosepiece

NI-ND6-I Intelligent DIC Sextuple Nosepiece

D-ND6 DIC Sextuple Nosepiece

C-NA Sextuple Nosepiece with Analyzer Slot

C-N Sextuple Nosepiece




*3L

FFE

FE

F

KKK’

OO

O

K

ab b

a

O

F

FF E

P

M

M

K

K’

N

N’

K

K

K

K’

KI S

NN’

J

J

l

GG G

PP

E

FE

M

S

λ

λ

W

W

T

T

T

U

V

2

3

4

2

3

4

T TUV

T TUV

T TUV

26 27

Specifications

Ni-E Ni-U

Focusing stage type Focusing nosepiece type

Mainbody

Opticalsystem CFI60infinityopticalsystem CFI60andCFI75infinityopticalsystems CFI60infinityopticalsystem

Focusing (strokefromfocuspoint)

ViamotorizedstageUp/Downmovement (Up2mm/Down13mm)

ViamotorizednosepieceUp/Downmovement (Up13mm/Down2mm)

ViamanualstageUp/Downmovement (Up3mm/Down26mm)

Built-inlinearencoder,Resolution:0.025μm Motorizedescapeandrefocusmechanism

CoaxialCoarse/Finefocusing

Illumination

Halogenlamp(12V100W) ·NI-ND-EMotorizedNDFilter(option) Halogenlamp(12V100W)

Built-infly-eyelens Built-inNCB11,ND8,ND32filters(detachable,oneadditionalfiltermountable)anddiffuser(non-detachable)ND2filter(option)

Controls

TransmittedlightOn/Offswitch,Intensitycontroldialwithpresetfunction Imagecapturebutton

Built-inmotorizedcontrolswitches —

·Stand-alonecontrolunitDS-L3·NI-ERGNIErgoController(option)

·Stand-alonecontrolunitDS-L3(option)·NI-SRCPSimpleRemoteControlPad (option)

Powersupplyunit ExternalpowersupplyNI-CTLAControlBoxAforallconfigurations

Built-inforhalogenlampNI-CTLBControlBoxBisnecessarywhenMotorized/Intelligentoptionsarecombined.

Eyepieces(F.O.V.mm) ·CFI10x(22)·CFI10xMPhotomask(22)·CFI12.5x(16) ·CFI15x(14.5)·CFIUW10x(25)·CFIUW10xMPhotomask(25)

Tubes

F.O.V.22mm (Eyepiece/Port)

·C-TBBinocularTube ·C-TE2ErgonomicBinocularTube(100/0,50/50withC-TEP2DSCPort) Inclinationangle:10-30degree,Extensionupto40mm

F.O.V.25mm*1 (Eyepiece/Port)

·C-TFTrinocularTubeF(100/0,0/100) ·C-TTTrinocularTubeT(100/0,20/80,0/100) ·C-TT-CTrinocularTube(100/0,0/100,forconfocal)*2 ·NI-TTQuadrocularTiltingTube(Eyepiece/Upperport/Rearport:100/0/0,0/100/0,0/0/100) Inclinationangle:15-35degree

·NI-TT-EMotorizedQuadrocularTiltingTube (Eyepiece/Upperport/Rearport:100/0/0,0/100/0,0/0/100) Inclinationangle:15-35degree

—

Ports(F.O.V.11mm)

·C-TEP2DSCPortforErgonomicBinocular(withC-mountadapter,0.7x)·NI-BPUBackPortUnit(withC-mountadapter,1.0x)·NI-RPZDSCZoomingPortforQuadrocularTube(withC-mountadapter,manualzoom,0.6x-2.0x)

·NI-RPZ-EMotorizedDSCZoomingPortforQuadrocularTube(withC-mountadapter,motorizedzoom,0.6x-2.0x) —

Arms·NI-SAMStandardArm

·NIE-CAMContactArm(forMotorized/Intelligentoptions) ·NIU-CAMContactArm(forMotorized/Intelligentoptions)

Nosepieces

Motorized ·NI-N7-EMotorizedSeptupleNosepiece ·NI-ND6-EMotorizedSextupleDICNosepiece —

·NI-N7-EMotorizedSeptupleNosepiece ·NI-ND6-EMotorizedSextupleDICNosepiece

Intelligent ·NI-N7-IIntelligentSeptupleNosepiece ·NI-ND6-IIntelligentSextupleDICNosepiece —

·NI-N7-IIntelligentSeptupleNosepiece ·NI-ND6-IIntelligentSextupleDICNosepiece

Manual

·D-ND6SextupleDICNosepiece ·C-NSextupleNosepiece ·C-NASextupleNosepiecewithAnalyzerSlot ·L-NU5UniversalQuintupleNosepieceESD·L-NBD5BDQuintupleNosepieceESD

·FN-S2NSlidingNosepiece (forCFI60objectives) Changeover2objectives,DICsliderinsertable ·FN-MN-HSingleObjectiveHolder (forCFI75objective) DICsliderinsertable

·D-ND6SextupleDICNosepiece ·C-NSextupleNosepiece ·C-NASextupleNosepiecewithAnalyzerSlot·L-NU5UniversalQuintupleNosepieceESD·L-NBD5BDQuintupleNosepieceESD

Ni-E Ni-U

Focusing stage type Focusing nosepiece type

Stages

·NIE-CSRR2RightHandleRotatableCeramic-coatedStagewith2SHolder Crosstravel78(X)x54(Y)mm Handleheightandtorqueadjustable

·FN-3PS2FN1StandardStage Crosstravel30(X)x30(Y)mm

·C-SR2SRightHandleStagewith2SHolder ·C-CSR1SRightHandleCeramic-coatedStagewith1SHolder ·C-CSRRightHandleCeramic-coated Stage ·NIU-CSRR2RightHandleRotatableCeramic-coatedStagewith2SHolder Crosstravel78(X)x54(Y)mm Handleheightandtorqueadjustable

·NI-S-EMotorizedXYStage Resolution:0.1μm ·NI-SH-DDishHolder(option)

Substages·NI-SSRSubstage (forMotorizedUniversalCondenserandRotatable/Motorizedstages)

·NI-SSFSubstageforFocusingNosepiece (forLWDcondenserandFN1Standard/Motorizedstages)

·NI-SSRSubstage(forRotatablestage) ·NI-SSSubstage(forNon-rotatablestages)

Condensers

Motorized

·NI-CUD-EMotorizedUniversalCondenserDry ForDIC,phasecontrast,darkfieldobservations AttachedonNI-SSRSubstage

— —

Manual

·NI-CUDUniversalCondenserDry ·C-CAbbeCondenserNA0.9 ·C-CAchromatCondenserNA0.9 ·DarkfieldCondenser(Oil) ·DarkfieldCondenser(Dry) ·C-CAchromat/AplanatCondenserNA1.4 ·C-CSlideAchromatCondenser2-100x ·C-CAchromatSwing-outCondenser1-100x ·AchromatSwing-outCondenser2-100x ·XLWDCondenser ·D-CUODICCondenserOil

·FN-CLWDCondenserNA0.78 (forDICandobliquelightillumination)

·NI-CUDUniversalCondenserDry ·C-CAbbeCondenserNA0.9 ·C-CAchromatCondenserNA0.9 ·DarkfieldCondenser(Oil) ·DarkfieldCondenser(Dry) ·C-CAchromat/AplanatCondenserNA1.4 ·C-CSlideAchromatCondenser2-100x ·C-CAchromatSwing-outCondenser1-100x ·C-CPhaseContrastTurretCondenser*3 ·AchromatSwing-outCondenser2-100x ·XLWDCondenser ·D-CUODICCondenserOil

Epi-fluorescence illuminator

Filtercubeturret

6filtercubesmountable,HighS/Nnoiseterminatormechanismforallturrets ·NI-FLT6-EMotorizedEpi-fluorescenceCubeTurret Motorizedshutter,Statuscheckfunction*4 ·NI-FLT6-IIntelligentEpi-fluorescenceCubeTurret Manualshutter,Statuscheckfunction*4 ·NI-FLT6Epi-fluorescenceCubeTurret Manualshutter

Lightdistributiondevice

·NI-FLEIEpi-fluorescenceAttachment Aperturediaphragmandfielddiaphragm(Centerable/Detachable),NDfilters(ND4,ND8,ND16) ·NI-PAUNiPhotoactivationUnit(405nmto650nmlasers)

Option

·NI-BAW-EMotorizedBarrierFilterWheel 7filtersmountable,0.2secbetweenadjacentpositions ·NI-EXW-EMotorizedExcitationFilterWheel 8filtersmountable,0.15secbetweenadjacentpositions ·NI-SH-EMotorizedShutter 0.012secbetweenopenandclosestate

·NI-SH-EMotorizedShutter 0.012secbetweenopenandclosestate

Epi-illuminationlightsource

·C-HGFI/HGFIEHGPrecenteredFiberIlluminatorIntensilight(130W) ·HgLamphouseandPowerSupply(100W) *2 ·XeLamphouseandPowerSupply(75W) *2 ·HalogenLamphouseandTransformer(100W) *2

Powerconsumption211W (withmax.halogenlampintensity andfullmotorizedoptions)

96W (withmax.halogenlampintensity andfullmotorizedoptions)

Mainbody:133W(withmax.halogenlampintensity) ControlBoxB:29W(withfullmotorizedoptions)

Weight(approx.)29kg (Epi-fluorescentconfigurationwithmotorizedquadroculartiltingtube)

42kg (Photoactivationconfigurationwithmotorizedquadroculartiltingtube)

20kg (Brightfieldconfigurationwithergonomicbinoculartube)

*1 When used with a double layer, such as with layered epi-fluorescence cube turrets, F.O.V. is 22. *2 Cannot be used with the focusing nosepiece type. *3 Can only be mounted on the NI-SS Substage. *4 Status check function: Status of Filter/Nosepiece etc. can be recorded with captured images and/or displayed on the controller monitor.

EnPrinted in Japan (1212-00)T Code No. 2CE-MQVH-4

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. December 2012 ©2011-12 NIKON CORPORATION

Company names and product names appearing in this brochure are their registered trademarks or trademarks.N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure shall be required in case of export from Japan.*Products: Hardware and its technical information (including software)Monitor images are simulated.

WARNINGTO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

This brochure is printed on recycled paper made from 40% used material.

NIKON CORPORATIONShin-Yurakucho Bldg., 12-1, Yurakucho 1-chome, Chiyoda-ku, Tokyo 100-8331, Japanphone: +81-3-3216-2375 fax: +81-3-3216-2385 http://www.nikon.com/instruments/

NIKON INSTRUMENTS INC.1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)fax: +1-631-547-0306http://www.nikoninstruments.com/

NIKON INSTRUMENTS EUROPE B.V.Tripolis 100, Burgerweeshuispad 101, 1076 ER Amsterdam, phone: +31-20-7099-000 fax: +31-20-7099-298http://www.nikoninstruments.eu/

NIKON INSTRUMENTS (SHANGHAI) CO., LTD.CHINA phone: +86-21-6841-2050 fax: +86-21-6841-2060(Beijing branch) phone: +86-10-5831-2028 fax: +86-10-5831-2026(Guangzhou branch) phone: +86-20-3882-0552 fax: +86-20-3882-0580

NIKON SINGAPORE PTE LTDSINGAPORE phone: +65-6559-3618 fax: +65-6559-3668

NIKON MALAYSIA SDN. BHD.MALAYSIA phone: +60-3-7809-3688 fax: +60-3-7809-3633

NIKON INSTRUMENTS KOREA CO., LTD.KOREA phone: +82-2-2186-8400 fax: +82-2-555-4415

NIKON CANADA INC.CANADA phone: +1-905-602-9676 fax: +1-905-602-9953

NIKON FRANCE S.A.S.FRANCE phone: +33-1-4516-45-16 fax: +33-1-4516-45-55

NIKON GMBHGERMANY phone: +49-211-941-42-20 fax: +49-211-941-43-22

NIKON INSTRUMENTS S.p.A.ITALY phone: +39-055-300-96-01 fax: +39-055-30-09-93

NIKON AGSWITZERLAND phone: +41-43-277-28-67 fax: +41-43-277-28-61

NIKON UK LTD.UNITED KINGDOM phone: +44-208-247-1717 fax: +44-208-541-4584

NIKON GMBH AUSTRIAAUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40

NIKON BELUXBELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45

Upright MicroscopeSeries+2CE-MQVH-4...Nikon has developed a new photoactivation unit for upright...

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