Two Dimensional NMR R. V. Hosur Department of Chemical ... iupab/WS_Nov24_2009_3.pdf · Department

download Two Dimensional NMR R. V. Hosur Department of Chemical ... iupab/WS_Nov24_2009_3.pdf · Department

of 24

  • date post

    23-Jun-2018
  • Category

    Documents

  • view

    213
  • download

    0

Embed Size (px)

Transcript of Two Dimensional NMR R. V. Hosur Department of Chemical ... iupab/WS_Nov24_2009_3.pdf · Department

  • Two Dimensional NMR

    R. V. Hosur

    Department of Chemical SciencesTata Institute of Fundamental Research

    Homi Bhabha Road, Mumbai

  • Two Dimensional NMR

    S(t1,t2) 2d FT

    S(F1, F2)

  • Two Dimensional NMR

  • A1 A2 X1 X2

    A1

    A2X1

    X2

    X X AA

    Coherence transfer

    Inverting X spin causes A1 to A2 interchange

    Inverting A spin causes X1 to X2 interchange

  • t1/2 t1/2 t2

    2D J-resolved Spectroscopy

    J J +

    Spin-Echo

    90 180

    Evolution Detection

    Chemical Shifts are refocused

  • J - separation

    2D Separation

  • 2D Correlation Spectroscopy

    NOESYInvestigation of exchange processes by two-dimensional NMR spectroscopyJ. Jeener, B. H. Meier, P. Bachmann, R. R. ErnstJ. Chem. Phys. 71, 4546-4553 (1979)

    COSYTwo Dimensional Spectroscopy: Application to nuclear magnetic resonanceW. P. Aue, E. Bartholdi, R. R. ErnstJ. Chem. Phys. 64, 2229- 2246 (1976)

  • .. COSY

  • Total Correlation Spectroscopy (TOCSY)

  • NOESY

    c >> 1 c

  • NOESY

  • 500MHz 2D NOESY spectrum

  • 1 mM Lysozyme

  • 500MHz 2D NOESY spectrum

  • Heteronuclear Single Quantum Coherence (HSQC)

  • FKBP

  • Receiver phase alternation cancels unwanted H magnetization not coupled to 13C

  • HMQC - TOCSY spectrum