Tom Misteli, Ph.D.

53
Spatial genome organization in the formation of translocations and in DNA repair Tom Misteli, Ph.D.

Transcript of Tom Misteli, Ph.D.

Page 1: Tom Misteli, Ph.D.

Spatial genome organization in the formation oftranslocations and in DNA repair

Tom Misteli, Ph.D.

Page 2: Tom Misteli, Ph.D.

aaggcgcgtcgagatgatcacgacggcatgctagctagccatacgcgtcaatcgtagctagtttactctagtacgatgctagctacgtacgtcatgatcgatcgatcgtagctagctagctagctagcagttgcgttgtgcaccccccccacttacgtagcatgctagctagctagctgtcagtcagtacgtgtacgtagctagctagtacgcagtacgcagttagcatcgtagcattttagccgcggctagctagtgcatgcatgctagctattagctacgtagctacgatgcatattactgatgctagctagctacgtaaggcgcggcattagctagctagctagctagccggcgctagctagctagtgatgtgtgtgtgtgcacacgtgtctgatattacgtacgtagctgtacgtagctagctcgtagctagtcagtacgtcagtaccgctatagctagctacgcgtaatctatatgctagctagcatgctagctagatattagctatcgtatggctagctagctagctagatgctacgtactacgtcatcatactgcagtactgctacgtcatgacgagtacgtatccagtcatgactgcatacgtcgtacgactgactgtctatcgtcatgtacgtcatgtactagtctagctagctagctacgatcgtacgatcgtagctagctagctacgtacgtgcatcatggcgtcagcgcgtagatgctagctagctagctagctagctagctacgtagcgcgcgctatattactatgcattagtcgatgctagctacgtacgtagctacgtacggtcagctacgtacgtcagtcaggcggcatgcattattcgacgtcgtatcgatgcatgcgtagctagtgctacgtacgatgctagtcgactgactcgatcgatcgatcgagtcgtactggtacgatgctagctacgatcgatgatgctacgtcggatttatatatattagagccggccggcggcgtagctagcattatatgcgtagcaaaacgatgcttagtagctagctagcgtatgactgactgcatagcgctacgtacgtacgtcagcgtcgatcgatgatgctagctagctagctgactgactcgatacgcatggtctgatctacgatcgatcgtacgatcgtgtgctagctagctagctagctagctagctagctacgtacgatcgtacgcgtgcgtagctgat

10 um nucleus

2m DNA

5x1012 cells/person1013 m DNA/person

100x distance fromEarth-Sun

Genomes exist in the cell nucleus

3 billion bp

Page 3: Tom Misteli, Ph.D.

aaggcgcgtcgagatgatcacgacggcatgctagctagccatacgcgtcaatcgtagctagtttactctagtacgatgctagctacgtacgtcatgatcgatcgatcgtagctagctagctagctagcagttgcgttgtgcaccccccccacttacgtagcatgctagctagctagctgtcagtcagtacgtgtacgtagctagctagtacgcagtacgcagttagcatcgtagcattttagccgcggctagctagtgcatgcatgctagggctgagagtcgtgagtgatgatggtagaaactagchaacgtacgtaggcgcggcattagctggcgnagtgtccgatgatggcagtgagttgaggcgatgggttaaatgacacgtgtctgatattacgtacgtagctgtacgtagctagctcgtagctagtcagtacgtcagtaccgctatagctagctacgcgtaatctatatgctagctagcatgctagctagatattagctatcgtatggctagctagctagctagatgctacgtactacgtcatcatactgcagtactgctacgtcatgacgagtacgtatccagtcatgactgcatacgtcgtacgactgactgtctatcgtcatgtacgtcatgtactagtctagctagctagctacgatcgtacgatcgtagctagctagctacgtacgtgcatcatggcgtcagcgcgtagatgctagctagctagctagctagctagctacgtagcgcgcgctatattactatgcattagtcgatgctagctacgtacgtagctacgtacggtcagctacgtacgtcagtcaggcggcatgcattattcgacgtcgtatcgatgcatgcgtagctagtgctacgtacgatgctagtcgactgactcgatcgatcgatcgagtcgtactggtacgatgctagctacgatcgatgatgctacgtcggatttatatatattagagccggccggcggcgtagctagcattatatgcgtagcaaaacgatgcttagtagctagctagcgtatgactgactgcatagcgctacgtacgtacgtcagcgtcgatcgatgatgctagctagctagctgactgactcgatacgcatggtctgatctacgatcgatcgtacgatcgtgtgctagctagctagctagctagctagctagctacgtacgatcgtacgcgtgcgtagctgat

Non-random genomeorganization

Nuclear compartments

Dynamics

Architectural elements

The complex cell nucleus

Page 4: Tom Misteli, Ph.D.

Non‐random spatial genome organization

Croft et al., JCB, 1999

18

18

1919

Meaburn & Misteli, Nature, 2007

Page 5: Tom Misteli, Ph.D.

Non‐random spatial genome organization

12 14

15

Parada et al., Cur. Biol., 2002

Meaburn & Misteli, Nature, 2007

Page 6: Tom Misteli, Ph.D.

Non‐random gene positioning

Roix & Misteli, Nat Gen., 2003

GFAPS100β

Takizawa and Misteli, G&D, 2008

Page 7: Tom Misteli, Ph.D.

Non‐random gene positioning

Roix & Misteli, Nat Gen., 2003

• Activity-dependent• Cell type-specific• Tissue-specific• Evolutionarily conserved• Differentiation• Development• Disease

Page 8: Tom Misteli, Ph.D.

Chromosome translocations

• Hallmark of cancer cells‐ Formation of fusion proteins‐ Gene misregulation

• Can be causal in tumor• Form by illegitimate joining of broken chromosomes

Fundamentally a spatial problem:Translocations require physical interaction of partners

Page 9: Tom Misteli, Ph.D.

Spatial positioning of translocation partners

12:14:15

80% of lymphomas contain translocations involving combinations of 12/14/15

Liyanage et al., Blood, 2000

Page 10: Tom Misteli, Ph.D.

12 14 15

1514 12

12

14 15

1

4

4

119

19

1

1

4

4

19

19

A cluster of chromosomes 12/14/15 in lymphocytes

Parada et al., Current Biology, 2002

Page 11: Tom Misteli, Ph.D.

Tissue‐specific translocations and tissue‐specific positions

Parada et al., Genome Biology, 2004

Page 12: Tom Misteli, Ph.D.

T(8;14) myc-Ig(H) 85% of patients

T(8;22) myc-Ig(λ) 10% of patients

T(8;2) myc-Ig(κ) <5% of patients

Roix et al., Nature Genetics, 2003

Proximity of translocation partners in Burkitt’s lymphoma

Page 13: Tom Misteli, Ph.D.

Proximity of translocation‐prone partners

HumanBurkitt’s lymphoma multiple partners

Chronic lymphocytic lymphoma multiple partners

Chronic myeloid leukemia  BCR – ABL

Promyelocytic leukemia PML – RAR

Papillary thyroid cancer  RET ‐ H4

Ewing sarcoma EWSR1 ‐ FLI1 

Anaplastic large cell lymphoma multiple partners

MouseLymphoma 12:14:15

Hepatoma 5:6

Page 14: Tom Misteli, Ph.D.

Chromosome intermingling and tranlsocations

From Branco & Pombo, PLoS Biology, 2006

Page 15: Tom Misteli, Ph.D.

Formation of chromosome translocations

Contact first

Breakage first

Page 16: Tom Misteli, Ph.D.

Formation of chromosome translocations

Contact first

DSBs are immobile

DSBs must be mobile

Breakage first

Page 17: Tom Misteli, Ph.D.

Mobility of DSBs

MobilityLisby et al., NCB, 2003Aten et al., Science, 2004

ImmobilityNelms et al., Science, 1998Kruhlak et al., JCB, 2005

Page 18: Tom Misteli, Ph.D.

GRI-SceI

TAGGGATAACAGGGTAAT ATCCCTATTGTCCCATTA

yeast endonuclease I-SceI

An experimental system to study chromosome ends in vivo

Soutoglou et al., Nature Cell Bio., 2007

Page 19: Tom Misteli, Ph.D.

GRI-SceI

TAGGGATAACAGGGTAAT ATCCCTATTGTCCCATTA

yeast endonuclease I-SceI

An experimental system to study chromosome ends in vivo

Soutoglou et al., Nature Cell Bio., 2007

Page 20: Tom Misteli, Ph.D.

An experimental system to study chromosome ends in vivo

γ-H2AXCFPlac RFP-I-SceI-GR MDC1 overlay

-TA

+TA

Soutoglou et al., Nature Cell Bio., 2007

Page 21: Tom Misteli, Ph.D.

The kinetics of induction of DSBs after TA additionRapid repair kinetics

Recruitment of repair factorsCutting (Ligation-mediated PCR)

Soutoglou et al., Nature Cell Bio., 2007

Page 22: Tom Misteli, Ph.D.

Analysis of the motion of free DNA endsDSB are positionally stable

Page 23: Tom Misteli, Ph.D.

Analysis of the motion of free DNA endsLocal separation of chromosome ends

intact broken

Page 24: Tom Misteli, Ph.D.

Perc

en

tag

e o

f ce

lls

wit

h

sep

ara

ted

arr

ays

DSB separation in the absence of Ku80

wt

Ku

80

kd

Ku80 mediates chromosome end stability

Soutoglou et al., Nature Cell Bio., 2007

Page 25: Tom Misteli, Ph.D.

High precision analysis of the mobility of broken DNA ends

+TA/Ku80 kd+TA

Increased mobility in the absence of Ku80

Page 26: Tom Misteli, Ph.D.

Identification of a recurrent translocation involving the arra

Kundan Sengupta, NCI

T(dic3, 8, 17)

Soutoglou et al., Nature Cell Bio., 2007

Identification of a recurrent array translocation

Page 27: Tom Misteli, Ph.D.

Proximity of array translocation partners

Chr 3 Chr 8 Chr 17 Chr 3 Chr 19

Soutoglou et al., Nature Cell Bio., 2007

Page 28: Tom Misteli, Ph.D.

Formation of chromosome translocations

Soutoglou and Misteli, Current Opinion in Cell Biology, 2007Misteli, Cell, 2007

Page 29: Tom Misteli, Ph.D.

Formation of chromosome translocations

Soutoglou and Misteli, Current Opinion in Cell Biology, 2007Misteli, Cell, 2007

Page 30: Tom Misteli, Ph.D.

Formation of chromosome translocations

Soutoglou and Misteli, Current Opinion in Cell Biology, 2007Misteli, Cell, 2007

Page 31: Tom Misteli, Ph.D.

Formation of chromosome translocations

Contact firstDSBs are immobile

Correlation between translocationfrequency and spatial proximity

Non-random spatial arrangementof the genome is a significantdeterminant of translocations

Soutoglou and Misteli, Current Opinion in Cell Biology, 2007Misteli, Cell, 2007

Page 32: Tom Misteli, Ph.D.

Tissue A Tissue B

Determinants of translocations: tissue‐specific genomes

Page 33: Tom Misteli, Ph.D.

Determinants of translocations: gene expression

Anaplastic large cell lymphoma

patients:

CSF1R FRA1  ID2NPM/ALK1

• patients with no translocations

2p 5q

Mathas et al., PNAS, in press

Page 34: Tom Misteli, Ph.D.

Determinants of translocations: gene expression

Anaplastic large cell lymphoma

control patient

patients:

CSF1R FRA1  ID2NPM/ALK1

2p 5q

• patients with no translocations

Mathas et al., PNAS, in press

Page 35: Tom Misteli, Ph.D.

Yeast vs. mammalian

Yeast ‐mobilityLisby et al., NCB, 2003Nagai et al., Science, 2008Kalocsay, Mol. Cell, 2009

Mammalian – immobilityNelms et al., Science, 1998Kruhlak et al., JCB, 2005Soutoglou et al., NCB, 2007butAten et al., Science, 2004Dimitrova et al., Nature, 2009

Soutoglou and Misteli, 2009Nature Reviews Mol Cell Bio

Page 36: Tom Misteli, Ph.D.

DSB repair

SensorsNbs1Mre11Rad50

TransducersMdc153BP1BRCA1/2

EffectorsATM-kinaseATR-kinaseDNAPK

DDR

Cell cyclecheckpoints

apoptosis senescence

Page 37: Tom Misteli, Ph.D.

Repair foci: cytological manifestations of DNA repair

Soutoglou and Misteli, 2009Nature Reviews Mol Cell Bio

What is the functional relevance of repair foci?How do they assemble? What  is their structure?

Page 38: Tom Misteli, Ph.D.

LacO

RepairfactorLacR

Bringing repair factors to chromatin

Page 39: Tom Misteli, Ph.D.

Activation of DDR by repair factor tethering

Soutoglou and Misteli, Science, 2008

Page 40: Tom Misteli, Ph.D.

Activation of DDR by repair factor tethering

Soutoglou and Misteli, Science, 2008

Page 41: Tom Misteli, Ph.D.

Activation of DDR by repair factor tethering

Soutoglou and Misteli, Science, 2008

Page 42: Tom Misteli, Ph.D.

Activation of DDR by repair factor tethering

sensors transducers effectors

Soutoglou and Misteli, Science, 2008

Page 43: Tom Misteli, Ph.D.

lacR-NBS1

lacR-NBS1

lacR-NBS1

lacR-NBS1

γ-H2AX

γ-H2AX

γ-H2AX

γ-H2AX

ATM inhibitor

DNAPK inhibitor

Caffeine

ControlATMDNA-PKcaffeine

DDR activation by tethering is ATM‐dependent

Soutoglou and Misteli, Science, 2008

Page 44: Tom Misteli, Ph.D.

Repair factor tethering leads to cell‐cycle delays

Soutoglou and Misteli, Science, 2008

Page 45: Tom Misteli, Ph.D.

Repair factor tethering leads to cell‐cycle delays

Phosphorylation of Rb

Soutoglou and Misteli, Science, 2008

Page 46: Tom Misteli, Ph.D.

Ligation-mediated PCR

Activation of DDR does not require DNA lesions

-DNA damage is not required to assemble the repair machinery-DNA damage is not required to propogate/maintain DDR

Soutoglou and Misteli, Science, 2008

Page 47: Tom Misteli, Ph.D.

LacO

RepairfactorLacR

X

Probing repair factor interplay

Page 48: Tom Misteli, Ph.D.

Interdependencies in repair factor recruitment

Tether

LacRNBS1MRE11MDC1ATMChk1Chk2

NB

S1

MR

E11

MD

C1

53BP

1A

TM

Tether

ProbeProbe

Downstream factors can recruit upstream components

Page 49: Tom Misteli, Ph.D.

Tether Probe

LacRNBS1MRE11MDC1ATMChk1Chk2

NB

S1

MR

E11

MD

C1

53BP

1A

TM

Tether

Probe

Interdependencies in repair factor recruitment

H2AX-/-

Downstream, but not upstream, recruitment eventsare dependent on H2AX

Page 50: Tom Misteli, Ph.D.

Amplification and spreading of DDR via cyclical recruitment

DDR

Amplification of DDR signalSpreading of repair machinery

DDRDDR

Page 51: Tom Misteli, Ph.D.

aaggcgcgtcgagatgatcacgacggcatgctagtagccatacgcgtcaatcgtagctagctagctagctttactctagtacgatgctagctacgtacgtcatgatcatcgatcgtagctagctagctagctagactacgcatcagttgcgttgtgcaccccccccacttacgtagcatgctagctagctagctgtcagtcagtacgagctacgattgtacgtagctagctagtacgcagtacgcagttagctcgtagcattttagccgcggctagctagctagctagcgtgcatgcatgctagctattagctacgtagctacgatcatattactgatgctagctagctacgtacgtacgtacaggcgcggcattagctagctagctagctagccggcgctagctagctagtgatgtgtgtgtgtgcacactgatcacacgtgtctgatattacgtacgtagctgtacgtagcagctcgtagctagtcagtacgtcagtacggctagctgccgctatagctagctacgcgtaatctatatgctagcagcatgctagctagatattagctatcgtacgtacgtagtggctagctagctagctagatgctacgtactacgtcatcatactgcagtactgctacgtcatgactgcagtacggagtacgtatccagtcatgactgcatacgtcgtac

Spatial genome organization and genome stability

Global  genome organization‐ Effect of non‐random organization‐Mechanisms of translocations

Local chromatin organization‐ Assembly of the repair machinery‐ Role of chromatin in assembly‐ Effect of chromatin on repair signaling

Page 52: Tom Misteli, Ph.D.

Reini LucoSteve MabonKaren MeaburnAlexandre MejatPaola ScaffidiGianluca PegoraroSara SnyderEvi Soutoglou (Strasbourg)Travis DittmerVassillis RoukosNicolas RascovanPilar SaladoresNard Kubben

Luis ParadaBilbao, Spain

Jeffrey RoixConstellation Pharma

Phil McQueenNIH

Maria JasinSloan Kettering

Thomas RiedAndre NussenzweigNIH

Page 53: Tom Misteli, Ph.D.