The role of apoptotic and necroptotic PBMC secretome in ... › hp › fileadmin › thorax... ·...
Transcript of The role of apoptotic and necroptotic PBMC secretome in ... › hp › fileadmin › thorax... ·...
January 2019
1
The role of apoptotic andnecroptotic PBMC secretome in angiogenesis and wound healing19.01.2019
OrganisationseinheitTitel der Präsentation ODER des Vortragenden
2
γ-irradiation activates the Necroptosis pathway
3
Experimental design
Presentation title / topic OR Presenter's name4
Positive selection of PBMC subsets
Peripheral blood mononuclear cells
• CD 14 Monocytes
• CD 4 T-Helper cells
• CD 8 Cytotoxic T-cells
• CD 56 Natural killer cells
• CD 19 B-cells
5
Irradiation and Necroptosis
6
p-MLKL
Presentation title / topic OR Presenter's name7
Presentation title / topic OR Presenter's name8
Presentation title / topic OR Presenter's name9
Presentation title / topic OR Presenter's name10
Presentation title / topic OR Presenter's name11
Presentation title / topic OR Presenter's name12
Presentation title / topic OR Presenter's name
13
Angiogenic potential of apoptotic and necroptotic PBMC?
14
PBMCNK-cellsMonocytes
B cellsCD8 T cellsCD4 T cells
PBMC
NK-cells
Monocytes
CD4 T cells
CD8 T cells
B cells
Angiogenesis
Cytokine production TGFβ pathway
Response to hypoxiap53 pathway
15
CD4 T cells
Monocytes
B cellsCD8 T cells
PBMC NK-cells
Presentation title / topic OR Presenter's name16
non-
irrad
iate
d
vehi
cle
z-VA
D
Nec
rost
atin
p-MLKL
c-cas 3
zVA
D+
Nec
rost
atin
γ-irradiation
vehicle zVAD
NecrostatinzVAD +
Necrostatin
Thank you for your attention!
Presentation title / topic OR Presenter's name17
Time and dose-dependent effects
18
p-MLKL
Scanning electron microscopy
19
-irradiated PBMC
Cell death
Biochemistry, Genetics and Molecular Biology » "Apoptosis and Medicine", book edited by Tobias M. Ntuli, ISBN 978-953-51-0701-9, Published: August 16, 2012 under CC BY 3.0 license. © The Author(s). Cell Death and Cancer, NovelTherapeutic Strategies By Silvina Grasso, M. Piedad Menéndez-Gutiérrez, et al DOI: 10.5772/51285
20