Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on...

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Supplementary Figure 1. ER stress-induced secretion of mature IL- 1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MSU 300 MSU 300 MSU 300 Mock R837 15 shRNA : Scramble NLRP3 ASC Tunica 10 15 20 Mock R837 15 10 15 20 Mock R837 15 10 15 20 Mock R837 15 10 15 20 Tunica Tunica Tunica Caspase-1 µg/ml SN xT IL-1 pIL-1 pCasp1 ASC NLRP3 CHOP Sup. Fig. 1

Transcript of Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on...

Page 1: Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MockR837 15 shRNA :ScrambleNLRP3ASC.

Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome.

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Page 2: Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MockR837 15 shRNA :ScrambleNLRP3ASC.

Supplementary Figure 2. ER stress-induced activation of the NLRP3 inflammasome is independent of TLR and RLR signaling.

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Page 3: Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MockR837 15 shRNA :ScrambleNLRP3ASC.

Supplementary Figure 3. IRE1α shutdown in shRNA-expressing THP-1 cells.

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Page 4: Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MockR837 15 shRNA :ScrambleNLRP3ASC.

Supplementary Figure 4. TRAF2 and ASK1 are dispensable for ER stress-induced NLRP3 inflammasome activation.

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Sup. Fig. 4


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