Supplementary Fig S1. Phase Contrast Photograph of Bacillus lonarensis sp. nov. 25nlgT

1 μm

Natronobacillus azotifigens NCCB 100215T (EU143681) Bacillus kochii WCC 4582T (FN995265)Bacillus oleronius DSM 9356T (X82492)

Bacillus acidicola 105-2 T (AF547209)Bacillus algicola KMM 3737T (AY228462)

Bacillus berkeleyi KMM 6244T (JN187498)Bacillus pseudofirmus DSM 8715T (X76439)

Bacillus murimartini LMG 21005T (AJ316316) Bacillus plakortidis P203T (AJ880003) Bacillus gibsonii DSM 8722T (X76446) Bacillus rhizosphaerae SC-N012T (FJ233848)

Bacillus clausii DSM 8716T (X76440) Bacillus lonarensis sp. nov. 25nlgT (HG934297)

Bacillus patagoniensis PAT 05T (AY258614) Bacillus xiaoxiensis JSM 081004T (HM054474)

Bacillus hunanensis JSM 081003T (HM054473)sequence Bacillus oshimensis K11T(AB188090)

Bacillus lehensis MLB2T (AY793550) Bacillus alkalinitrilicus ANL-iso4T (EF422411)

Bacillus arseniciselenatis DSM 15340T (AJ865469) Bacillus alkalidiazotrophicus MS6T (EU143680)

Bacillus macyae JMM-4T (AY032601) Anaerobacillus alkalilacustris Z-0521T(DQ675454)

Bacillus nanhaiisedminis NH3T (GQ292772) Bacillus halodurans ATCC 27557T(AB021187)

Bacillus hemicellulosilyticus C-11T(AB043846) Bacillus bogoriensis LBB3T (AY376312)

Bacillus alcalophilus DSM 485T (X60603) Bacillus trypoxylicola SU1T (AB434284)

Bacillus pseudalcaliphilus DSM 8725 T(X76449) Bacillus alakisediminis K1-25T (AJ606037)

Bacillus akibai 1139T (AB043858) Bacillus krulwichiae AM31DT(AB086897)

Bacillus okhensis Kh10-101T (DQ026060) Bacillus wakoensis N-1T(AB043851)

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Supplementary Fig S2.ML Tree

Supplementary Fig S3.ME Tree

Natronobacillus azotifigens NCCB 100215T (EU143681) Bacillus kochii WCC 4582T (FN995265)

Bacillus oleronius DSM 9356T (X82492) Bacillus acidicola 105-2 T (AF547209)

Bacillus berkeleyi KMM 6244T (JN187498) Bacillus algicola KMM 3737T (AY228462)

Bacillus pseudofirmus DSM 8715T (X76439) Bacillus gibsonii DSM 8722T (X76446)

Bacillus murimartini LMG 21005T (AJ316316) Bacillus plakortidis P203T (AJ880003) Bacillus rhizosphaerae SC-N012T (FJ233848)

Bacillus clausii DSM 8716T (X76440) Bacillus lonarensis sp. nov. 25nlgT (HG934297)

Bacillus patagoniensis PAT 05T (AY258614) Bacillus xiaoxiensis JSM 081004T (HM054474)Bacillus oshimensis K11T(AB188090)

Bacillus hunanensis JSM 081003T (HM054473)Bacillus lehensis MLB2T (AY793550)

Bacillus alkalinitrilicus ANL-iso4T (EF422411) Bacillus alkalidiazotrophicus MS6T (EU143680)

Anaerobacillus alkalilacustris Z-0521T(DQ675454) Bacillus macyae JMM-4T (AY032601)

Bacillus arseniciselenatis DSM 15340T (AJ865469) Bacillus krulwichiae AM31DT(AB086897) Bacillus wakoensis N-1T(AB043851)

Bacillus okhensis Kh10-101T (DQ026060) Bacillus akibai 1139T (AB043858) Bacillus alakisediminis K1-25T (AJ606037)

Bacillus nanhaiisedminis NH3T (GQ292772) Bacillus halodurans ATCC 27557T(AB021187)

Bacillus hemicellulosilyticus C-11T(AB043846) Bacillus bogoriensis LBB3T (AY376312)

Bacillus alcalophilus DSM 485T (X60603) Bacillus trypoxylicola SU1T (AB434284)

Bacillus pseudalcaliphilus DSM 8725 T(X76449)

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65

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Natronobacillus azotifigens NCCB 100215T (EU143681)

Bacillus oleronius DSM 9356T (X82492)

Bacillus acidicola 105-2 T (AF547209)

Bacillus kochii WCC 4582T (FN995265)

Bacillus algicola KMM 3737T (AY228462)

Bacillus berkeleyi KMM 6244T (JN187498)

Bacillus pseudofirmus DSM 8715T (X76439)

Bacillus murimartini LMG 21005T (AJ316316)

Bacillus plakortidis P203T (AJ880003)

Bacillus gibsonii DSM 8722T (X76446)

Bacillus rhizosphaerae SC-N012T (FJ233848)

Bacillus clausii DSM 8716T (X76440)

Bacillus lonarensis sp. nov. 25nlgT (HG934297)

Bacillus patagoniensis PAT 05T (AY258614)

Bacillus xiaoxiensis JSM 081004T (HM054474)

Bacillus oshimensis K11T(AB188090)

Bacillus hunanensis JSM 081003T (HM054473)

Bacillus lehensis MLB2T (AY793550)

Bacillus alkalinitrilicus ANL-iso4T (EF422411)

Bacillus arseniciselenatis DSM 15340T (AJ865469)

Bacillus macyae JMM-4T (AY032601)

Bacillus alkalidiazotrophicus MS6T (EU143680)

Anaerobacillus alkalilacustris Z-0521T(DQ675454)

Bacillus hemicellulosilyticus C-11T(AB043846)

Bacillus halodurans ATCC 27557T(AB021187)

Bacillus bogoriensis LBB3T (AY376312)

Bacillus pseudalcaliphilus DSM 8725 T(X76449)

Bacillus trypoxylicola SU1T (AB434284)

Bacillus nanhaiisedminis NH3T (GQ292772)

Bacillus alakisediminis K1-25T (AJ606037)

Bacillus akibai 1139T (AB043858)

Bacillus krulwichiae AM31DT(AB086897)

Bacillus okhensis Kh10-101T (DQ026060)

Bacillus wakoensis N-1T(AB043851)

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Bacillus alcalophilus DSM 485T (X60603)

Supplementary Fig S4.MP Tree

A B

Supplementary Fig. S5. AP-PCR amplicon band patterns

A = Bacillus lonarensis sp. nov. 25nlgT

B= Bacillus patagoniensis PAT 05T

Bacillus patagoniensis PAT 05T

2nd d

imen

sion

1st dimension

DPG DPG

Bacillus lonarensis sp. nov. 25nlgT

PG

PG

PE

PL1 PL2

L1 L3

L2

Supplementary Fig. S6.

DPG

DPG

PG

PG

PE

PEPL1APL1

L1

PL2

L3

L2

L4

L5L6

APL2 APL2

Supplementary Fig. S7 (Polar lipid stains)

2nd d

imen

sion

1st dimension

(A) (B)

A= Bacillus lonarensis sp. nov. 25nlgT ; B=Bacillus patagoniensis PAT 05T

7.5 8 8.5 9 9.5 10

7.5 8 8.5 9 9.5 10Ab

unda

nce

Time (min)

(A)

(B)

MK-7

MK-6

Supplementary Fig S8. Quinone

A=Bacillus patagoniensis PAT 05T ; B= Bacillus lonarensis sp. nov. 25nlgT

Supplementary Fig. S9 (Cell wall amino acids)

A=Bacillus patagoniensis PAT 05T ; B=Strain Bacillus lonarensis sp. nov. 25nlgT

(A) (B)

D-Glutamate

Meso-DAP

DL- Alanine