Superactivity of α–Chymotrypsin with Biological Buffers ... · Superactivity of...
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Superactivity of α–Chymotrypsin with Biological Buffers, TRIS, TES, TAPS, and TAPSO in Aqueous Solutions Bhupender S. Gupta a , Mohamed Taha b and Ming Jer Lee a a Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 106-07, Taiwan b CICECO, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal Supporting Information Fig. 1S (a-c). The UV-Visible measurements for α–CT in 0.05 M, 0.5 M, and 0.7 M or 1.0 M TES (a), TAPS (b), and TAPSO (c) at 25 °C and pH = 8.0. Solid black line 0.05 M, solid red line 0.5 M, and solid blue line 1.0 M. 200 225 250 275 300 325 350 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Absorbance Wavelength (nm) (a) 200 225 250 275 300 325 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Absorbance Wavelength (nm) (b) 200 225 250 275 300 325 350 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Absorbance Wavelength (nm) (c) Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2014
Transcript of Superactivity of α–Chymotrypsin with Biological Buffers ... · Superactivity of...
Superactivity of α–Chymotrypsin with Biological Buffers, TRIS, TES, TAPS, and TAPSO in Aqueous Solutions
Bhupender S. Guptaa, Mohamed Tahab and Ming Jer Leea
a Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 106-07, Taiwan
b CICECO, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal
Supporting Information
Fig. 1S (a-c). The UV-Visible measurements for α–CT in 0.05 M, 0.5 M, and 0.7 M or 1.0 M TES (a), TAPS (b), and TAPSO (c) at 25 °C and pH = 8.0. Solid black line 0.05 M, solid red line 0.5 M, and solid blue line 1.0 M.
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Electronic Supplementary Material (ESI) for RSC Advances.This journal is © The Royal Society of Chemistry 2014
Fig. 2S (a-c). The emission fluorescence spectra for α–CT in 0.05 M, 0.5 M, and 0.7 M or 1.0 M TES (a), TAPS (b), and TAPSO (c) at excited wavelength of 280 nm, 25 °C temperature and pH = 8.0: (■), 0.05M; (●), 0.5 M: (▲), 0.7 or 1.0 M
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Fig. 3S (a). The hydrogen bond interactions of TES with α–CT
Fig. 3S (b). The hydrogen bond interactions of TAPS with α–CT
Fig. 3S (c). The hydrogen bond interactions of TAPSO with α–CT
