Sup. Figure 1

P35S:AtIBH1SRDX

P35S:GUS

CaMV35S----------------------------NOSGUS

CaMV35S-Ω-------------------------------------NOSAtIBH1 SRDX

GLDLDLELRLGFA

PGLN:anti-HBs478LC-P35S:anti-HBs478HC

CaMV35S---------------------NOS478HC ERGLN--------------rbc-478LC

Figure S1. Schematic representation of the constructs introduced into tobacco plants. The P35S:GUS constructs contain the protein coding region of GUS (GUS; gray box) driven by the CaMV 35S promoter. PGLN:anti-HBs478LC-P35S:anti-HBs478HC constructs contain the protein-coding region for the antibody against hepatitis B virus protein No. 478. The L chain (478LC; open box) was driven by the tobacco glucanase promoter (GLN) and the H chain (478HC; open box) was driven by the CaMV 35S promoter.

Wild-type

#1 #2 #9 #12 #13 #150

0.4

0.8

1.2

Relative

expression

(At2g43060/ actin)

WT #1 #2 #9 #12 #13 #15

AtIBH1sx

Figure S2. The dwarf phenotype depends on expression of P35S:AtIBH1SRDX in tobacco.Upper photo shows tobacco plants of wild type (wt) and P35S:AtIBH1SRDX (AtIBH1sx). Bar = 15 cm. Lower panel shows relative expression of the AtIBH1-SRDX transgene in each P35S:AtIBH1SRDX transgenic tobacco plant. Transcript levels of AtIBH1 were normalized to Actin expression.

Sup. Figure 2

Wild

type

Hei

ght

Leng

thWidth

Figure S3. Measurements of length, width, and height of transgenic tobacco plants.

Sup. Figure 3