Sunniest C18-HT 2um

biotech Ab presents Sunniest C18-HT 2μm

biotech in Partnership with

HPLC column

Biotech SunnieSt c18-ht 2 μm

Reatures• Lowbackpressureandhighefficiencybypreciselyclassified

particle

• Highpressurepacking(10,000psi)usinghardsilicagelswithhighpressureresistant•leadslongcolumnlifewithoutanyvoid.

• UniquebondingtechniqueforSunniest(patentpending)

• Themostsuitableinnersurfaceofcolumnbyspecialgrinding

Conventional2μmsilicagelparticlewasclassifiedagain.20%volumewascutofffrombothsidesrespectively.Consequentlycolumnbackpressurereducedmorethan15%.Our2μmsilicagelparticleshowsahalfpressuretocomparewiththeothersub-2μmsilicagelparticle.

Itisdifficulttoend-caponsub2μmor2μmsilicagelparticleaswellas3μmor5μmsilicagelparticle.Mostsub2μmor2μmC18columnsshowsmallerplatenumberandhighertailingfactorthanSunniestC18-HT.SunniestC18-HT2μmshowsgoodpeakshapeforamitriptylineundernotonlymetahnol/phosphatebuffermobilephasebutalsoacetonitrile/phosphatebuffermobile.

Column: SunniestC18-HT,2μm50x2.1mmMobilephase: CH3OH/H2O,CH3CN/H2OFlowrate: 0.5mL/minTemperature: 40ºC

Column: SunniestC18-HT,2mm50x2.0mmMobilephase:A)CH3OH/20mMPhosphatebufferpH7.5=80/20B)CH3CN/20mMPhosphatebufferpH7.0=60/40Flowrate: 0.4mL/minPressure: A)19.5MPa,B)13.5MPaTemperature: 40ºCSample: 1=Uracil,2=Propranolol, 3=Nortriptyline,4=Amitriptyline,

Nortriptyline

Measured by Coulter Counter method Column pressure using methanol or acetonitrile and water

Sunniest technique to 2 μm particle!

narrow Particle DiStriBution anD low Back PreSSure

• an unique MoDification (Patent PenDing aS SunnieSt SerieS)

Retention time (min)

3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2

B Acetonitrile Other C18

Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26

TF: USP tailing factor

1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）

Concentration of organic solvent (%)

Narrow particle distribution

Conventional particle Classified particle

1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061

Comparison of tailing factor

Comparison of plate number


3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061




3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061




3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061




3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061




3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061




3 4

4

1

2

A Methanol

3

4

N(4) =7,529

TF(4) =1.19 4

1 2


Other C18

N(4) =7,670

TF(4) =1.50 N(4) =8,068

TF(4) =1.13

N(4) =3,068

TF(4) =4.26


1.0 2.0

Methanol

Acetonitrile

D10: 1.75 mmD50: 2.01 mmD90: 2.31 mmD90/D10: 1.32

D10: 1.67 mmD50: 2.09 mmD90: 2.65 mmD90/D10: 1.59

( )*'

Pres

sure（

MPa）




1.13 1.57

1.63 1.49

1.72 1.58

8,068 6,209

5,785 6,008 6,108

5,061


Comparison of plate numberUracil Propranolol

Amitriptyline

Biotech SunnieSt c18-ht 2 μm

2μmparticleallowstoreduceretentiontimebecausehighefficiencyiskeptunderhighflowrateconditions.Asshowntheabovechromato-grams,analyticaltimereduced1/8withoutsac-rificesofseparationbyusing2μm,50x2.1μmcolumninsteadof5μm150x4.6μmcolumn.

Itisveryimportantfor2μmparticletohaveacapacitytoresistpressurebecauseofhighcolumnbackpressure.Thelargeraporevolumeofsilicagel,theweakeracapacitytoresistpressure.Thesilicagelwith0.85ml/gofporevolumeisusedforSunni-estC18-HT,2μm,sothatithaveahighcapacitytoresistpressureandahighoperatingpressure.

SiliCa gel SuppoRt C18

packings particle size pore diameterSpecific sur-

face areaCarbon content

Bonded phase

Maximum operating pressure available pH range

SunniestC18-HT 2.0μm(Coultercounter)

10nm 340m2/g 16% C18 70MPaor10,000psi

1.5-10

Column: SunniestC18-HT,2mm50x2.0mmMobilephase:A)CH3OH/20mMPhosphatebufferpH7.5=80/20B)CH3CN/20mMPhosphatebufferpH7.0=60/40Flowrate: 0.4mL/minPressure: A)19.5MPa,B)13.5MPaTemperature: 40ºCSample: 1=Uracil,2=Propranolol, 3=Nortriptyline,4=Amitriptyline,

Mobilephase: CH3CN/0.1%Formicacid=20/80Flowrate: 1.0mL/minfor150x4.6mm 0.6mL/minfor50x2.1mmTemperature: 40ºCDetection: UV@230nmSample: 1=Acetaminophen,2=Antipyrine, 3=Aspirin,4=Ethenzamide

Mobilephase: CH3CN/H2O=60/40Flowrate: 0.6mL/minfor2.1x30mmand2.1x50mm 1.0mL/minfor3.0x50mm 0.4mL/minfor2.1x75mmand2.1x100mmTemperature: 40ºCDetection: UV@250nmSample: 1=Uracil,2=Toluene, 3=Acenaphthene,4=Butylbenzene

SeParation of analgeSicS

characteriSticS of SunnieSt c18-ht 2 μm

coMPariSon of Plate no.

Sunniest C18 5 m, 150 x 4.6 mm

1

2 3

12

4

Retention time (min) 10

Sunniest C18-HT 2 m, 50 x 2.1 mm

N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4

4321Retention time (min)

0


1

2 3

12

4



N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4


0

Ethenzamide


1

2 3

12

4



N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4


0


1

2 3

12

4



N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4


0

Acetaminophen


1

2 3

12

4



N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4


0

Antipyrine


1

2 3

12

4



N(4)=175,000 plate/m

N(4)=80,000 plate/m

High throughput

30 x 2.1 mm N(3)= 5,375

50 x 2.1 mm N(3)= 9,294

50 x 3.0 mm N(3)= 9,303

75 x 2.1 mm N(3)= 14,021

100 x 2.1 mm N(3)= 19,802


4

1

1

1

1

1

2

2

2

2

2

3

3

3

3

3

4

4

4

4


0

Aspirin

g company Y company S1 company S2 company W company ChromaNik technologies

Ra 0.34μm 0.32μm 0.37μm 0.03μm 0.20μm 0.01μm

Rz 1.88μm 1.62μm 1.91μm 0.19μm 0.90μm 0.10μm

biotech Ab

P.O. BOx 133

SE-439 23 OnSala

SwEdEn

tEl +46 (0)300-56 91 80

fax +46 (0)300-56 91 81

www.biotech.se

narrow Particle DiStriBution anD low Back PreSSure

orDering inforMation of SunnieSt c18-ht 2 μm

Center line Ra R

Schematic diagram of surface roughness

parameter of surface roughnessRa:Averageroughnessfromcenterline

Rz:Roughnesscalculatedfrom10pointsaverage(5pointsofmaximumand5pointsofminimum)

Itisconsideredthatsurfaceroughnessaffectscol-umnperformance.SurfaceasperityofChromaNikTechnologiescolumnis1/30to1/20tocomparewiththatofGLSciences,YMC,ShimadzuandWaters

columns.ChromaNIkTechnologiesprovidesacolumnwithverysmoothsurfacewhichisthemostsuitablefor2μmparticlepacking.

InnersurfaceofcolumnofA,B,Ccompanies InnersurfaceofChromaNikTechnologies

2μm

particle

2μm

particle

length (mm) 30 50 75 100

inner diameter (mm) Cat. No. Cat. No. Cat. No. Cat. No.

2.1 EB1931 EB1941 EB1951 EB1961

3.0 EB1331 EB1341 EB1351 EB1361

Sunniest C18-HT 2um

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