Stabilitas Vitamin A &amp

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    Stabilitas Vitamin A & Karoten, dan

    Diskusi Ilmiah:Kelayakan Fortifikasi VitaminApada Minyak GorengSEAFASTCenterIPBSenin,12Juli 2010

    Stabilitas VitaminA& Karoten,danPengembangan Minyak Sawit Merah

    Nuri Andarwulan,PhDSEAFAST C t IPB

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    SEAFASTCenter,IPBSoutheastAsianFood&Agr.Sci &TechCenter

    Departemen Ilmu dan Teknologi Pangan,IPB

    Agenda

    Stabilitas Vitamin AStabilitas VitaminA Pengembangan Minyak Sawit Merah

    Stabilitas karoten Penyerapan karoten

    Studi keamanan karoten Studi keamanan karoten

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    RedPalmOil/Minyak Sawit Merah

    degumming

    netralisasi

    bleaching

    deodorisasi

    CPOCPO

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    Hilang/kerusakan karoten selamapengolahan Modifikasi proses

    deodorisasi

    fraksinasi

    Karakterisasi NDRPO (Neutralized-Deodorized-Red Palm Oil)

    Parameter Nilai Pembanding 1 Pembanding 2****

    Kadar asam lemak bebas( b i l it t) (%)

    0.160.01 Maks 0.1* 0.2300.03(sebagai palmitat) (%)Kadar air (%) 0 Maks 0.1* 0

    Bilangan Peroksida(meq/1000g)

    6.510.01 0* 0.360.30

    -karotena (ppm) 336.343.20 370.1** 337.4340.53

    Dien terkonjugasi (%) 0.1810.004 1.540.02***

    TBA (mg malonaldehydeeqivalen/kg)

    0.020.01 0.9320.038***

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    Total tokoferol termasuktokotrienol (ppm)

    543.683.20 534.68**

    *dalam RBDPO (Basiron 2005), **dalam minyak sawit merah (Manorama et al. 1993),

    ***dalam RBDPOlein (Che Man dan Tan 1999), **** dalam NDRPO (Riyadi, 2009)

    (Ayustaningwarno,2010)

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    Kinetika degradasi karoten di MSM

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    5,9

    )

    y=0,0004x+5,7914R=0,9575

    5 4

    5,5

    5,6

    5,7

    5,8

    sentrasiBe

    taKaroten

    (ppm

    )

    60oC

    75oC

    90oC

    Linear(60oC)

    Linear(75oC)

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    y=0,0009x+5,7343R=0,9720

    y=0,0020x+5,7250R=0,9710

    5,2

    5,3

    5,4

    0 50 100 150 200 250 300 350 400 450 500

    LnKo

    ns

    LamaPenyimpanan(jam)

    Linear(90oC)

    (Ayustaningwarno,2010)

    y=6.685,336662x+12,190549R = 0 999986

    6,4

    6,2

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    (/jam)

    Kinetika degradasi karoten di MSM

    R =0,999986

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    7,8

    7,6

    7,4

    7,2

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    6,8

    6,6

    LnKon

    stan

    taLajuRe

    aksi

    lnk

    Linear(lnk)

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    1/Suhu(K)

    k=197258.5exp(6685.34/T)

    (Ayustaningwarno,2010)

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    ts=[Ln(Q0/Qs)]/k (Ross 1998) ts= lama penyimpanan

    Q il i l

    Kinetika degradasi karoten di MSM

    Q0= nilai mutu awal Qs= Nilai mutu akhir yang dapat diterima k= konstansa kecepatan reaksi

    tingginya nilai R2 dari model yang didapatkan, maka model tersebut dapat digunakan sebagai prediksi umur simpanNDRPO.

    Dengan asumsi kadar -karotena awal adalah 500 ppm danbatas akhir adalah 400 ppm dengan kondisi penyimpanan 30

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    pp g p y poC, dengan rumus Arrhenius dapat diketahui konstantakecepatan reaksi sebesar 5.22 x10-5 kemudian dengan rumuspenurunan mutu ordo 1 dapat diketahui lama penyimpananyaitu 4275 jam atau setara dengan 5.9 bulan.

    (Ayustaningwarno,2010)

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    15Rao,2000

    RedpalmoiltocombatvitaminAdeficiencyindevelopingcountries

    CRukminiFoodNutr Bull1994;15:1269

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    IncorporationofRedPalmOilinEdibleOil

    RPO foodhadastrong,unpleasantsmellandtastetaste.

    BlendofRPO(612%)incookingoil theblendwaspalatableandnoobjectionablesmellortasteoffoods.RPOdidimpartsomeredcolortotheoil.

    The blend containing 3070 ppm of caroteneTheblendcontaining30 70ppm ofcarotene(vitaminApotency3xthatofbutter).

    Potatochipsretained5001000gofcarotene/100g

    17Rao,2000

    RecommendationoftheNutritionAdvisoryCommitteeoftheIndianFundResearchAssociation(nowtheIndianCouncilofMedicalResearch)(Rao,2000)

    1937(3rd meeting) RPOreplacecodliveroilinthe treatment of keratomalacia RPO obtainedthetreatmentofkeratomalacia.RPOobtainedfromElaeis guineensis.

    4th meeting VitaminAcontentofRPObedetermined attheNutritionResearchLabandexplorethesuitablemixofRPOwithotheroils.

    5th meeting Studies were done on the keeping

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    5 meeting Studiesweredoneonthekeepingqualityofmixtures.Whenexposedtolight,themixwerebleachedwithin11months,butthelosswaslessifitwaskeptinatincontainer.

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    Rao,2000

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    Degradationofcarotene(Tannembaum etal.1985)

    Polymers,volatilecompounds,shortchainwatersolublecompounds

    O2

    Alltranscarotene

    carotene5,6epoxide Mutachrome

    Lightcatalyzedoxidation

    Chemicaloxidation

    NeoCarotenesBandU(cis form,38%vitaminAactivity)

    MXyleneToluen2,6dimethylnaphthalene

    Cooking&canningHightemperature

    Fragmentationproducts;e.g ionene

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    Effectsofepoxycarotenoids,betacarotene,andretinoicacidonthedifferentiationandviabilityoftheleukemiacelllineNB4invitro

    Duitsman,PK:Barua,AB:Becker,B:Olson,JAIntJVitamNutrRes.1999Sep;69(5):3038

    Abstract

    Threealltransepoxides ofbetacarotene(betaCar),namely,5,6epoxybetacarotene(5,6EC),5,8epoxybetacarotene(5,8EC)and5,6,5',6'diepoxybetacarotene(5,6,5',6'DEC)weresynthesizedbytreatmentofbetacarotenewith3chloroperoxybenzoicacid,werepurifiedchromatographically,andwerecharacterized.Therelativepotencies(mean+/ S.D.)of1microM compoundsininducingthedifferentiationofNB4cells,acelllinethatcontainsthechromosomaltranspositiont(15;17)characteristicofacutepromyelocytic leukemia,after4daysofincubationwere:RA:1.35+/ 0.16,5,6EC:0.29+/ 0.01,5,8EC:0.22+/ 0.05,5,6,5',6'DEC:0.11+/ 0.02,betaC:0.09+/ 0.01,andthecontrol:0.06+/ 0.01.Thesameorderofpotenciesexistedatotherconcentrationstestedandatotherincubationtimes.Pvaluesforthedifferencesbetweentheinducingactivitiesofsuccessivepairsofcompoundsat1microM were:RAvs.5,6EC,lessthan0.001;5,6ECvs.5,8EC,lessthan0.01;5,8ECvs.5,6,5',6'DEC,lessthan0.01;5,6,5',6'DECvs.betaCar,lessthan0.10;betaCarvs.control less than 0 005 Similar P values were also obtained for studies at other concentrations andcontrol,lessthan0.005.SimilarPvalueswerealsoobtainedforstudiesatotherconcentrationsandatotherincubationtimes.Theviablecellmassat4dayswasinverselyproportionaltotheextentofdifferentiation(rs =1.0).Theinducingactivitiesofallcompoundsweredosedependent.Thus,the5,6monoepoxideofbetacarotene,whichhasnotpreviouslybeenstudiedasaninducer,showedhigheractivityinNB4celldifferentiationthanthe5,8monoepoxide,the5,6,5',6'diepoxide,orbetacarotene.Possibleexplanationsoftheseobservationsarediscussed

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    ReversephasegradientHPLCprofilesofcarotenoids inhumanplasma

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    Thank YouThank You

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