x

for ES・iPS cells research

Small molecules Ver.2

Featured Products！

CultureSure® Y-27632 /

Appearance： White ～ pale yellow, crystalline powder ～ powder

Assay (HPLC)： min. 98.0%

Solubility： water、ethanol

Specific rotation[α]D20(c=0.5,CH3OH)： +6.3° (measured value)

Mycoplasma tested

Endotoxins：less than 0.25EU/mg

Cytotoxicity checked※

ROCK Inhibitor

Ito, H., et al.: Liver Int., 32, 592 （2012）. Kawamata, M., et al.: Proc. Natl. Acad. Sci. USA., 107, 14223 （2010）. Claassen, DA., et al.: Mol. Reprod. Dev., 76, 722（2009）. Martin-Ibanez, R., et al.: Hum. Reprod., 23, 2744（2008）. Watanabe, K., et al.: Nat. Biotechnol., 25, 681（2007）. Sakamoto, K., et al.: J. Pharmacol. Sci., 92, 56（2003）. Nishimaru, K., et al.: J. Pharmacol. Sci., 92, 424（2003）. Uehata, M., et al.: Nature, 389, 990 （1997）.

References

CAS No. 331752-47-7 C14H21N3O・2HCl・H2O=338.27

★ Described in P.5 ★

※ human iPS cells 201B7 strain was used

CultureSure® 10mmol/l Y-27632 Solution, Animal-derived-free (liquid)

Formulation：10 mM solution of Y-27632 in water

Mycoplasma tested

Endotoxins：less than 0.78EU/ml (measured value)

Sterility tested

Y-27632, MF

Y-27632, MF has been registered in Drag Master File in

Japan (MF). We manage raw materials, conduct the

validations of the manufacturing process and analytical tests

and manufacture in the system obtaining permanently stable

quality products.

Y-27632, MF

Registered in MF※1 Registration No. 227MF40013 Made in Japan Manufacturing all from synthesis to packaging in Japan Animal-Free Chemical synthetic products of non-use of animal-derived raw materials High quality-stability Continuous multiple lot pass record

Wako holds the only license from API Corporation (Chiyoda-ku, Tokyo, Japan) to manufacture, sell, and supply Y-27632 for research purposes (as of October 1, 2014).

Appearance ： White ～ pale yellow, crystalline powder ～ powder

Assay (HPLC) ： min. 98.0%

Solubility ： water 、ethanol

Specific rotation[α]D20(c=1.0,CH3OH)： +2.0°～+10.0°

Mycoplasma tested ※2

Endotoxins：less than 0.25EU/mg ※2

Viable cell count tested※2

CultureSure® Series is a group of products that have undergone

endotoxin testing, mycoplasma testing, and cytotoxicity

checking to verify its safe use for cell culture applications.

This product is a ready to use stock solution. It has been

sterilized by filtration and is used as it is.

Code No. Package

030-24021 036-24023 034-24024

1mg 5mg

25mg

Code No. Package

039-24591 300μL

Code No. Package

259-00613 257-00614

5mg 25mg

※1 It shall not be assumed that the validity and assurance of the drug substance’s quality and adequacy are officially approved by MF registration. ※2 Mycoplasma test, Endotoxins test and Viable cell count test are product specification tests in each lot, but are not included in MF registration items

CultureSure® Y-27632 (powder) Y-27632, MF (powder)

Y-27632 Selective and strong inhibitor of ROCK. Y-27632 inhibits contraction of the vascular smooth muscle, infiltration of cancer cells, and regulation of cell differentiation caused by a signal transduction system of ROCK. Y-27632 enhances post-cryopreservation survival and cloning efficiency of human ES cells and human iPS cells. [ROCK：Rho-associated coiled-coil forming kinase / Rho binding kinase/ Serine/threonine kinase protein phosphoenzyme]

CultureSure® A-83-01

CultureSure® CKI-7 Dihydrochloride

ALK4,5,7 Inhibitor

CK1 Inhibitor

■Powder

■Powder

It has been reported that when added to rat iPS cell culture medium,

it facilitates uniform growth of rat iPS cells without differentiation, and

can be cultivated over a long period.

Use of CKI-7 in the presence of SB431542 and Y-27632 is reported to

induce human ES cells and iPS cells to differentiate into retinal

progenitor cells in the serum-free and feeder-free conditions.

CAS No. 1177141-67-1 C11H12ClN3O2S・2HCl=358.67

CultureSure® A419259 Trihydrochloride

Src-family Kinase Inhibitor

CAS No. 1435934-25-0 C29H34N6O・3HCl = 592.00

Appearance ：White ～ pale yellowish red, crystalline powder ～

powder

Assay (HPLC)： min. 98.0%

Solubility ：water、DMSO、ethanol、acetonitrile+water(1:1)

Mycoplasma tested

Endotoxins ： less than 0.1EU/mg

Src-family kinase is known to be important for signaling cascade for

embryogenesis, differentiation, and cell proliferation.

Wnt Inhibitor

■Powder

IWP-2 inactivates porcupine (Porcn), and suppresses palmitylation

of the Wnt protein. It has been reported that IWP-2 makes

human iPS cells differentiate into cardiomyocyte.

★ Described in P.7★

CultureSure® IWP-2

CAS No. 686770-61-6 C22H18N4O2S3=466.60

■Powder

CultureSure® CHIR99021 GSK-3β Inhibitor

CHIR99021 has been reported that differentiation can be suppressed

with high efficiency when ES cells are cultured in a medium containing

CHIR99021 and PD0325901.

■Powder

■Reference Ying, QL., et al .: Nature, 453, 519 (2008).

CAS No. 252917-06-9 C22H18Cl2N8=465.34

■Liquid

Formulation：10 mM solution of CHIR99021 in DMSO

Mycoplasma tested

Endotoxins：less than２EU/ml (measured value)

Sterility tested

■Liquid

Formulation：3 mM solution of CKI-7 Dihydrochloride in water

Mycoplasma tested

Endotoxins：less than 1EU/ml (measured value)

Sterility tested

Takahashi, M. et al.: J. Cell Sci., 122, 3169 (2009). ■Reference

Li, W., et al.: Cell Stem Cell, 4, 16（2009）. ■Reference

Minami, I. et al.: Cell Rep., 2, 1448 (2012). ■Reference

★ Described in P.3 ★

Appearance ：White ～ yellow, crystalline powder ～ powder or mass

Assay (HPLC) ： min. 98.0%

Solubility ： DMSO

Mycoplasma tested

Endotoxins ： less than 0.01EU/mg

Cytotoxicity checked※

※ human iPS cells 201B7 strain was used

Appearance ： White ～ pale brown, crystals ～ powder

Assay (HPLC) ： min. 98.0%

Solubility ： DMSO

Mycoplasma tested

Cytotoxicity checked※

Appearance ：White ～ yellow, crystalline powder ～ powder or

mass

Assay (HPLC) ： min. 98.0%

Solubility ： DMSO

Mycoplasma tested

Endotoxins ： less than 0.01EU/mg

Cytotoxicity checked※

Appearance ： White ～ pale brown, crystalline powder ～ powder

Assay (HPLC) ： min. 98.0%

Solubility ： water

Mycoplasma tested

Endotoxins ： less than 0.05EU/mg

Cytotoxicity checked※

★ Described in P.6★

★ Described in P.3★

★ Described in P.6★

CAS No. 909910-43-6 C25H19N5S=421.52

We cannot sell this product to United States for its patent.

2｜ Small molecules for ES・iPS cells research

Product Name Grade Package Code No.

CAS No.

2mg 039-24111

10mg 035-24113

909910-43-6

1mg 012-23021

10mg 018-23023

446859-33-2

BIX01294 for Cellbiology 2mg 023-16401

935693-62-2

1mg 021-17041

5mg 027-17043

856925-71-8

6-Bromoindirubin-3'-oxime【BIO】【GSK-3 InhibitorⅨ】 for Cellbiology 1mg 029-16241

667463-62-9

25mL 029-05393

500mL 023-05396

107-92-6

1mg 038-23101

5mg 034-23103

100mg 032-23104

252917-06-9

CultureSure® 10mmol/l CHIR99021 DMSO Solution, Animal-derived-free for Cell Culture 300μL 038-24681

252917-06-9

Cyclic Pifithrin-α Hydrobromide for Cellbiology 5mg 036-24001

511296-88-1

3-Deazaneplanocin A Hydrochloride 【DZNep】 for Cellbiology 1mg 049-33701

120964-45-6

10mg 041-30101

25mg 047-30103

48208-26-0

A reversible inhibitor of p53-mediated apoptosis and p53-dependent gene transcription. A decrease in p53 activity enhances the

reprogramming efficiency of human and mouse somatic cells.

This product is histone methyltransferase EZH2 inhibitor. This compound has been reported to enable the efficient production of

iPS cells solely based on compounds by combining with valproic acid, CHIR99021, 616452 (ALK-5 Inhibitor), Tranylcypromine,

Forskolin, DZNep, and TTNPB. 〔Hou, P., et al ., Science , 341(6146), 651 (2013).〕

A DNA methyltransferase inhibitor. Inhibits cell proliferation with low cytotoxicity.

〔Tsumura, A., et al .: Genes Cells , 11, 805 (2006).〕

Action Mechanism

An ALK4/ALK5/ALK7 inhibitor. It is useful for long-term homogeneous culture of rat and dog iPS cells without differentiation.

Endotoxin tested, mycoplasm tested, cytotoxicity checked. 〔Li, W., et al .: Cell Stem Cell , 4, 16 (2009).〕

A TGF-β R I kinase/ALK5 inhibitor. Can be used as substitute of Sox2 or c-Myc (Yamanaka 4 factors) in induction of murine iPS cells.

〔Maherali N., et al .: Curr. Biol ., 19, 1718 (2009).〕

A histone methyltransferase inhibitor. Enhances the efficiency of iPS cell induction when used during delivery of Oct3/4 and Klf4

to neural stem cells. Use of this product with (±) BayK8644 during delivery of Oct3/4 and Klf4 to fibroblasts also increases the

efficiency of iPS cell induction.

〔Shi, Y., et al .: Cell Stem Cell , 2, 525 (2008).〕〔Shi, Y., et al .: Cell Stem Cell , 3, 568 (2008).〕

A myosin II-specific inhibitor. Inhibits blebbing in human ES cells and human iPS cells dissociated from the colony and prevents

apoptosis. 〔Ohgushi, M., et al .: Cell Stem Cell , 7, 225 (2010).〕

A GSK-3 inhibitor. Maintains the differentiation potential of human and murine ES cells in feeder cell-free culture medium. 〔Sato,

N., et al. : Nat. Med. , 10, 55 (2004).〕

Improves iPS cell induction efficiency. 〔Liang, G., et al .: J. Biol. Chem ., 285, 25516 (2010).〕

CHIR99021 is GSK-3β inhibitor, which selectivity is high. This product does not show cross-reactivity to CDKs (Cyclin-dependent

kinases). It has been reported that differentiation can be suppressed with high efficiency when ES cells are cultured in a medium

containing CHIR99021 and PD0325901. Endotoxin tested, mycoplasma tested, cytotoxicity checked.

〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

This product is a ready to use 10mmol/l CHIR99021 stock solution filtered. No animal-derived matters are used in the process of

manufacturing.

ALK5 Inhibitor 【TGF-βRⅠ Kinase InhibitorⅡ】 for Cellbiology

（ -） -Blebbistatin for Cellbiology

DNA Methyltransferase Inhibitor 【RG108】 for Genetic Research

CultureSure® CHIR99021

CultureSure® A-83-01 【TGF-βRⅠ Kinase InhibitorⅣ】 for Cell Culture

for Cell Culture

Butyric Acid Wako Special Grade

Small molecules for ES・iPS cells research｜3

Maintenance of Undifferentiated Potential and Enhancement of Reprograming Efficiency of ES・iPS Cells

P.2

P.2

P.2

4｜ Small molecules for ES・iPS cells research

Product Name Grade Package Code No.

CAS No.

EHNA Hydrochloride for Cellbiology 10mg 056-08221

58337-38-5

GF 109203X for Biochemistry 1mg 079-03811

133052-90-1

H1152 Dihydrochloride for Cellbiology 1mg 088-09281

871543-07-6

HA-100 Hydrochloride for Cellbiology 10mg 086-10071

141543-63-7

IM-12 for Cellbiology 5mg 091-07131

1129669-05-1

IQ-1 for Cellbiology 5mg 095-05951

331001-62-8

1mg 110-00831

5mg 116-00833

142273-20-9

Ki16425 for Cellbiology 5mg 115-01001

355025-24-0

5mg 162-25291

25mg 168-25293

391210-10-9

10mmol/l PD0325901 DMSO Solution for Cell Culture 300μL 166-25951

391210-10-9

PD173074 for Cellbiology 5mg 160-26831

219580-11-7

PD184352 for Cellbiology 5mg 165-26761

212631-79-3

10mmol/l PD184352 DMSO Solution for Cell Culture 300μL 163-25961

212631-79-3

PD-98059 for Biochemistry 5mg 169-19211

167869-21-8

PS48 for Cellbiology 10mg 164-26851

1180676-32-7

Kenpaullone for Cellbiology

A PDK1 inhibitor. Use with Oct4 and Klf4 during induction of iPS cells from neonatal human epithelial keratinocytes (NHEKs)

increases reprogramming efficiency by 15-fold. Adding it with sodium butyrate enhances induction efficiency by 25-fold.

〔Zhu, S., et al .: Cell Stem Cell , 7, 651 (2010).〕

PD0325901 for Cellbiology

Binds to Wnt-3a and maintains proliferation and pluripotency of murine ES cells without serum.

〔Miyabayashi, T., et al .: Proc. Natl. Acad. Sci. USA ., 104, 5668 (2007).〕

An inhibitor of GSK-3 and CDKs. Can be used as substitute of Klf4 (one of Yamanaka 4 factors) during iPS cell induction. Adding it

during delivery of Yamanaka 4 factors will also enhance reprogramming efficiency.

〔Lyssiotis, CA., et al .: Proc. Natl. Acad. Sci. USA ., 106, 8912 (2009).〕

A LPA receptor antagonist.

A MAPK inhibitor. Use with CHIR99021 enables efficient culture of ES cells while maintaining the differentiation potential. Use

with SB431542 and thiazovivin also enhances reprogramming efficiency to human iPS cells by over 200-fold and increases

reprogramming speed. 〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

This product is a ready to use 10mmol/l PD0325901 stock solution filtered.

An FGFR inhibitor. Stimulates self-proliferation of murine ES cells. 〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

A MEK1 inhibitor. Use with CHIR99021 and SU5402 enables efficient culture of murine ES cells while maintaining the

differentiation potential. 〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

This product is a ready to use 10mmol/l PD184352 stock solution filtered.

A MAPK inhibitor. Promotes self-proliferation of ES cells.

〔Burdon, T., et al .: Dev. Biol ., 210, 30 （1999）.〕〔Hamazaki, T., et al .: Mol. Cell Biol., 26, 7539 （2006）.〕

A phosphodiesterase/adenosine deaminase inhibitor. Can be used as substitute of bFGF. Inhibits human ES cell differentiation

efficiently and reversibly without feeder cells and maintains pluripotency.

〔Burton, P., et al .: Biochem. J ., 575, 432 (2010).〕

Action Mechanism

A protein kinase C (PKC) inhibitor. Use with U0126 stablely maintains the differentiation potential of human ES cells and iPS cells.

〔Kinehara, M., et al .: PLoS ONE , 8, e54122 (2013).〕

A ROCK inhibitor. Has a stronger inhibitory effect than Y-27632.

A PKC inhibitor. Use with PD0325901, CHIR99021, A-83-01 and LIF improve the reprograming efficiency in feeder-free condition,

when iPS cells are produced. 〔Yu, J., et al .,:PLoS One ., 6, 3, e17557 (2011).〕

A selective inhibitor of GSK-3β (glycogen synthase kinase 3β ). Naive human ES cells, which resemble mouse ES cells in the ground

state, can be induced and maintained with the concomitant use of PD0325901, IM-12, SB590855, Y-27632, WH-4-023, hLIF, and

activin A on feeders.〔Theunissen T.W., et al .: Cell Stem Cell , 15(4), 471(2014).〕

Product Name Grade Package Code No.

CAS No.

1mg 199-16551

5mg 195-16553

152121-47-6

SB203580 Hydrochloride for Cellbiology 1mg 198-16761

869185-85-3

SC-1 【Pluripotin】 for Cellbiology 1mg 191-15411

839707-37-8

25g 193-01522

500g 197-01525

156-54-7

1mg 197-16731

5mg 193-16733

25mg 191-16734

215543-92-3

1mg 202-18011

5mg 208-18013

1226056-71-8

10mmol/l Thiazovivin DMSO Solution for Cell Culture 300μL 204-19551

1226056-71-8

U0126 for Biochemistry 5mg 211-01051

109511-58-2

5g 227-01071

25g 225-01072

99-66-1

WH-4-023 for Cellbiology 5mg 234-02741

837422-57-8

1mg 257-00511

5mg 253-00513

25mg 251-00514

1g 257-00516

331752-47-7

1mg 030-24021

5mg 036-24023

25mg 034-24024

331752-47-7

ROCK inhibitor. Enhances post-cryopreservation survival and cloning efficiency of human ES cells and human iPS cells.

〔Ito, H., et al .: Liver Int ., 32, 592 (2012).〕〔Kawamata, M., et al .: Proc. Natl. Acad. Sci. USA ., 107, 14223 (2010).〕

〔Claassen, DA., et al .: Mol. Reprod. Dev ., 76, 722 (2009).〕〔Martin-Ibanez, R., et al .: Hum. Reprod ., 23, 2744 (2008).〕

〔Watanabe, K., et al .: Nat. Biotechnol ., 25, 681 (2007).〕〔Sakamoto, K., et al .: J. Pharmacol. Sci ., 92, 56 (2003).〕

〔Nishimaru, K., et al .: J. Pharmacol. Sci ., 92, 424 (2003).〕〔Uehata, M., et al .: Nature , 389, 990 (1997).〕

Endotoxin tested, mycoplasma tested cytotoxicity checked.

A RasGAP and ERK1 inhibitor. Maintains the differentiation potential of murine ES cells in LIF-free, feeder cell-free, and serum-free

culture medium. 〔Chen, S., et al .: Proc. Natl. Acad. Sci. USA ., 103, 17266 （2006）.〕

A histone deacetylase inhibitor. Promotes self-proliferation of ES cells.

〔Ware, CB., et al .: Cell Stem Cell , 4, 359 (2009).〕

An FGFR1 inhibitor. Use with CHIR99021 and PD184352 enables efficient culture of murine ES cells while maintaining the

differentiation potential. 〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

It has been reported that use of this product in combination with SB431542 and PD0325901 in the preparation of human iPS cells

not only improves reprogramming efficiency 200 times or more but also speeds up reprogramming.

〔Lin T., et al .: Nat. Methods , 6, 805 (2009).〕

This product is a ready to use 10mmol/l Thiazovivin stock solution filtered.

MEK1/2 inhibitor. Use with GF109203X maintain undifferentiated state of human ES/iPS cells stably.

〔Ying, QL., et al .: Nature , 453, 519 (2008).〕

Histone deacetylase inhibitor. Enhances efficiency of iPS cell induction by Oct4 , Klf4 and Sox2 .

〔Huangfu, D., et al .: Nature Biotechnol ., 26, 1269 (2008).〕

Y-27632 for Cellbiology

CultureSure® Y-27632 for Cell Culture

Inhibitor of Lck(Lymphocyte-specific protein tyrosine kinase) and Src kinase. Naive human ES cells, which resemble mouse ES cells

in the ground state, can be induced and maintained with the concomitant use of PD0325901, IM-12, SB590855, Y-27632, WH-4-

023, hLIF, and activin A on feeders. 〔Theunissen T.W., et al .: Cell Stem Cell , 15(4), 471(2014).〕

A MAPK inhibitor. Used for isolation and maintenance of ES cells.

〔Qi, X., et al .: Proc. Natl. Acad. Sci. USA ., 101, 6027 (2004).〕

A MAPK inhibitor. Used for isolation and maintenance of ES cells.

〔Qi, X., et al .: Proc. Natl. Acad. Sci. USA ., 101, 6027 (2004).〕

Action Mechanism

Valproic Acid for Biochemistry

for Cellbiology

Sodium Butyrate -

SB203580 for Cellbiology

Thiazovivin for Cellbiology

SU5402

Small molecules for ES・iPS cells research ｜5

P.2

Product Name Grade Package Code No.

CAS No.

1mg 034-24801

5mg 030-24803

25mg 038-24804

100mg 034-24806

1435934-25-0

5mg 011-25291

25mg 017-25293

844499-71-4

100mg 015-22531

1g 011-22533

2627-69-2

Am580 for Biochemistry 5mg 014-16631

102121-60-8

AMD3100 Octahydrochloride for Cellbiology 10mg 014-25421

155148-31-5

100mg 035-16303

200mg 039-16301

59865-13-3

Ciglitazone for Cellbiology 5mg 030-20981

74772-77-3

CKI-7 Dihydrochloride for Cellbiology 5mg 034-21501

1177141-67-1

CultureSure® CKI-7 Dihydrochloride for Cell Culture 5mg 035-23971

1177141-67-1

Action Mechanism

A casein kinase (CK1) inhibitor. Use with SB431542 and Y-27632 induces differentiation of human ES cells and human iPS cells into

retinal progenitor cells in serum- and feeder cell-free culture medium.

〔Takahashi, M., et al .: J. Cell Sci ., 122, 3169 (2009).〕

A-769662 for Cellbiology

CultureSure® A419259 Trihydrochloride for Cell Culture

Ciclosporin A for Biochemistry

AICAR for Cellbiology

Src-family kinase inhibitor. Src-family kinase is known to be important for signaling cascade for embryogenesis, differentiation,

and cell proliferation. We cannot sell this product to United States for its patent.

AMP-activated protein kinase activator. Activation of AMPK inhibits signal transmission of mTOR (mammalian target of rapamycin)

that promotes cell growth, proliferation and survival.

Endotoxin tested, mycoplasma tested, cytotoxicity checked.

An AMPK activator. Suppresses differentiation into fat cells and induces differentiation of neural stem cells into astroglia.

〔Giri, S., et al .: Nutr. Metab . (Lond), (2006).〕〔Zang, Y., et al .: J. Biol. Chem ., 283, 6201 (2008).〕

Synthetic Retinoids. More rapidly and efficiently induce huan ES/iPS cells into nephrogenic intermediate mesoderm.

〔Araoka, T., et al .: PLoS One ., 9, e84881 (2014).〕

CXCR4 antagonist. It effectively recruits hematopoietic stem cells in vivo . 〔Lura, A., et al ., : Exp Hematol ., 39(3), 384 (2011).〕

Enhances the induction efficiency of mouse and human iPS cell differentiation into cardiac muscle and cardiac progenitor cells.

〔Fujiwara, M., et al .: PLoS. One , 6, e16734 (2006).〕

A PPARγ agonist. Induces differentiation of human mesenchymal stem cells into fat cells and inhibits differentiation into

osteoblasts. 〔Benvenuti, S., et al. : J. Endocrinol. Invest ., 30, RC26 (2007).〕

6｜ Small molecules for ES・iPS cells research

Differentiation Induction of ES・iPS Cells

Product Name Grade Package Code No.

CAS No.

5mg 259-00613

25mg 257-00614

331752-47-7

5mmol/l Y-27632 Solution for Cell Culture 300μL 253-00591

331752-47-7

CultureSure® 10mmol/l Y-27632 Solution, Animal-derived-free for Cell Culture 300μL 039-24591

331752-47-7

Y-27632, MF for Cell Culture

This product is registered in the Drug Master File in Japan. Endotoxin tested, mycoplasma tested and Viable cell count tested.

This product is a ready to use 5mmol/l Y-27632 stock solution filtered.

This product is a ready to use 10mmol/l Y-27632 stock solution filtered. No animal-derived matters are used in the process of

manufacturing.

Action Mechanism

P.2

P.2

P.2

Product Name Grade Package Code No.

CAS No.

CultureSure® 3mmol/l CKI-7 Dihydrochloride Solution,

Animal-derived-freefor Cell Culture 1mL 039-24611

1177141-67-1

5mg 043-33581

25mg 049-33583

208255-80-5

250mg 047-33861

1g 043-33863

25g 045-33862

50-02-2

1mg 044-33751

5mg 040-33753

866405-64-3

1mg 041-33761

5mg 047-33763

1219168-18-9

DMH1 for Cellbiology 10mg 041-33881

1206711-16-1

10mg 067-02191

25mg 063-02193

66575-29-9

IPA-3 for Cellbiology 5mg 092-07041

42521-82-4

5mg 034-24301

25mg 030-24303

686770-61-6

IWR-1-endo for Cellbiology 5mg 094-06381

1127442-82-3

5mg 129-04861

10mg 125-04863

25mg 123-04864

154447-36-6

PluriSIn1 for Cellbiology 10mg 165-27501

91396-88-2

Purmorphamine for Cellbiology 5mg 166-23991

483367-10-8

Activator of adenylate cyclase. Use with bFGF induce mesenchymal stem cells into neuronal cells.

〔Jang, S. et al. : BMC Cell Biol ., 11, 25 (2010).〕

A PAK inhibitor. Shows particularly high selectivity to PAK1. PAK1 is associated with tumor development and metastasis.

Activates porcupine (Porcn), a membrane-bound o-acyltransferase (MBOAT), and suppresses palmitylation of Wnt proteins, which

in turn inhibits phosphorylation of Wnt-dependent Lrp6 receptor and accumulation of Dvl2 and β catenin. Endotoxin tested,

mycoplasma tested, cytotoxicity checked.

A Wnt signaling inhibitor. Stabilizes β catenin-degradation complex (comprised of Axin2, Apc, Ck1, and Gsk3β) and stimulates

degradation of β catenin.

A phosphatidylinositol 3-kinase inhibitor. Induces differentiation of murine ES cells into insulin-producing cells.

DAPT 【γ-Secretase InhibitorⅨ】 for Cellbiology

This product is a ready to use 3mmol/l CKI-7 Dihydrochloride stock solution filtered. No animal-derived matters are used in the

process of manufacturing.

Action Mechanism

Dexamethasone

for Cellbiology

AMPK and BMP signal inhibitor. Induces differentiation of murine ES cells into cardiac muscle cells.

〔Hao, J., et al .: PLoS. One ., 3, e2904 (2008).〕

A selective inhibitor of BMP.　It dose not act on AMPK, ALK5, VEGFR2 and PDGFR.This induces efficient differentiation of mouse ES

cells into myocardial cells.　〔AO A., et al .: PLoS One , 7, e41627 (2012).〕

A SCD1 (Stearoyl-CoA desaturase-1) inhibitor. It selectively removes undifferentiated cells that still remain in culture cells

differentiated from human pluripotent stem cells (hPSCs). SCD1 inhibition triggers endoplasmic reticulum stress in

undifferentiated hPSCs, which in turn induces apoptosis. 〔Ben-David, U., et al .: Cell Stem Cell , 12,167 (2013).〕

A hedgehog signal agonist. Induces differentiation of mesenchymal progenitor cells and preosteoblastic cells into osteoblasts.

〔Wu, X., et al .: Chem. Biol ., 11, 1229 （2004）.〕

Forskolin for Biochemistry

for Cell Culture

A γ-secretase inhibitor. Inhibits Notch signaling and induces differentiation of ES cells from embryoid body to neuron. 〔Nelson,

BR., et al .: Dev. Biol ., 304, 479 (2007).〕〔Crawford, TQ., et al .: Dev. Dyn ., 236, 886 (2007).〕

Regulates bone differentiation of mesenchymal stem cells. 〔Jaiswal, N., et al .: J. Cell. Biochem .,64, 295 (1997).〕

AMPK and BMP signal inhibitor. Induces differentiation of murine ES cells into cardiac muscle cells.

〔Hao, J., et al .: PLoS. One ., 3, e2904 (2008).〕

CultureSure® IWP-2 for Cell Culture

LY294002 for Biochemistry

Dorsomorphin for Cellbiology

Dorsomorphin Dihydrochloride

Small molecules for ES・iPS cells research ｜7

P.2

P.2

Listed products are intended for laboratory research use only, and not to be used for drug, food or human use. / Please visit our online catalog to search for other products from Wako; http://www.e-reagent.com / This leaflet may contain products that cannot be exported to your country due to regulations. / Bulk quote requests for some products are welcomed. Please contact us.

Product Name Grade Package Code No.

CAS No.

50mg 186-01114

100mg 182-01116

250mg 182-01111

1g 188-01113

302-79-4

Reversine for Cellbiology 5mg 184-02391

656820-32-5

Shz-1 for Cellbiology 5mg 196-18021

326886-05-9

250mg 198-09811

1g 194-09813

71-44-3

1mg 203-17561

5mg 209-17563

58880-19-6

5mg 209-19481

50mg 205-19483

97322-87-7

1mg 206-17671

5mg 202-17673

601514-19-6

5mg 247-00951

25mg 243-00953

100mg 241-00954

284028-89-3

Troglitazone for Cellbiology

TWS119 【GSK-3β InhibitorⅩⅡ】 for Cellbiology

all -trans -Retinoic Acid for Biochemistry

Spermine for Biochemistry

Induces differentiation into nerve cells.

A human A3 adenosine receptor agonist. Induces dedifferentiation of myoblasts into stem cells.

〔Chen, S., et al. : J. Am. Chem. Soc ., 126, 410 (2004).〕

Action Mechanism

This promotes repair damage to cardiac muscles using stem cells. Further, it induces cardiac markers, including Nkx2.5.

〔H Sadek et al . Proc. Natl. Acad. Sci. USA. , 105(16), 6063 (2008).〕

Induces differentiation into cardiac muscle cells. 〔Sasaki, T., et al .: Differentiation , 76, 1023 (2008).〕

A histone deacetylase inhibitor. Induces differentiation into cardiac muscle cells.

〔Oyama, T., et al .: J. Cell. Biol ., 176, 329 (2007).〕

A PPARγ agonist. Induces differentiation of human mesenchymal stem cells into fat cell and inhibits differentiation into

osteoblasts. 〔Benvenuti, S., et al .: J. Endocrinol. Invest ., 30, RC26 (2007).〕

XAV939 for Cellbiology

A GSK-3β inhibitor. Induces differentiation of murine ES cells into nerve cells.

〔Ding, S., et al .: Proc. Natl. Acad. Sci. USA ., 100, 7632 (2003).〕

An inhibitor of Wnt/β-catenin pathway. Inhibits tankyrases 1 and 2, accelerates degradation of β-catenin and stabilization of axin

and, thus, inhibits Wnt pathway. Wnt signal is known to be important for self-proliferation of various stem cells. Therefore,

inhibitors of Wnt/β-catenin pathway are used in researches of various stem cells including ES cells and iPS cells.

Trichostatin A for Cellbiology

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