September 26 , 2011
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Dynamic materials for controlling the cell microenvironment and drug release April M. Kloxin, Assistant Professor Chemical Engineering and Materials Science and Engineering College of Engineering September 26, 2011
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Dynamic materials for controlling the cell microenvironment and drug release April M. Kloxin, Assistant Professor Chemical Engineering and Materials Science and Engineering College of Engineering. September 26 , 2011. Hydrogels for controlled cell culture. - PowerPoint PPT Presentation
Transcript of September 26 , 2011
Dynamic materials for controlling the cell microenvironment and drug releaseApril M. Kloxin, Assistant ProfessorChemical Engineering and Materials Science and Engineering College of Engineering
September 26, 2011
Hydrogels for controlled cell culture
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human mesenchymal stem cellsLive/Dead stain (Scale, 100 μm)
What are the important microenvironment signals for directing cell function in tissue regeneration or disease progression?
AM Kloxin, JA Benton, KS Anseth, Biomaters 31 (2010) 1-8AM Kloxin, MW Tibbitt, KS Anseth, Nature Protocols 5 (2010) 1867-1887AM Kloxin et al., Advanced Materials 22 (2010) 61-66
Biomaterials approach to understanding and directing cell function
Biomaterials development
Responsive
Materials
Ligament
Fibrocartilage
Bone
Tissue regenerationor disease treatment
1. Complex tissue regeneration
2. Model system and targets for disease regulation
3. In situ multiple-trigger drug delivery
Fundamental biological questions
1. MSC homing, function, and fate
2. Breast cancer cell dormancy/activation
3. Size- & time-scales for optimal drug release
AM Kloxin, CJ Kloxin, CN Bowman, KS Anseth, Adv Materials 22 (2010) 3484-3494MW Tibbitt and KS Anseth, Biotech Bioeng 103 (2009) 655-663Image (right): ES Place, ND Evans, MM Stevens, Nature Materials 8 (2009) 457-470
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Responsive materials platform for dynamic cell culture
• Property control on multiple – size scales: nano-, micro-, and meso-scale– time scales: seconds/minutes, hours, and days
• Appropriate physical structure and chemical functionalities
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Biomimetic tethers include peptide ECM mimics, cytokines, and growth factorsSimilar platform can be used for controlled drug release with multiple triggers
AM Kloxin, AM Kasko, CN Salinas, KS Anseth, Science 324 (2009) 59-63BD Fairbanks, …, KS Anseth, Adv Mater 21 (2009) 5005-5010
Our Facilities & Team
Dynamic Materials &Regenerative MedicineAprilKloxinGroup.org
Eric MacedoMatthew Rehmann
CollaboratorsKenneth van Golen (UD)Kristi Kiick (UD)David Colby (UD)Raja Sivamani (UC-Davis)
FundingPilot Project, NIH COBRE P20-RR016458Pilot Project, NIH COBRE P20-RR017716
Offices207 Colburn129 Colburn (students)
Materials & Assays 220 & 221 Colburn
Cell Culture 126 Colburn (Colby Lab)
The Group
