Roche kemiinstrumentFunktioner och mätprinciperLouise Levander, IBL Grundkurs i allmänkemi, 2016-09-09

Roche DiagnosticsHistoria

Roche kemiinstrumentUtformade för att passa alla typer av lab

Roche kemiinstrumentNomenklatur

c111

c311

c501

c502

c701

c702

e411

e601

e602

e801

cobas 4000 cobas 6000 cobas 8000

Roche kemiinstrumentAnalysmeny

α1-Acid Glycoprotein

α1-Antitrypsin

α1-Microglobulin

β2-Microglobulin

Apolipoprotein A-1

Apolipoprotein B

ASLO

T-Uptake*

D-Dimer

AT III

BicarbonatAmmonia

Albumin BCG

Albumin BCP

LDL-C

HDL-C Cholesterol Triglycerides

TiglyceridesGB

Bilirubin Direct

Bilirubin Total

Fructosamine

Creatinineenzymatic

Amikacin

Quinidine

Gentamycin

Carbamazepine

Acetaminophen

Cyclosporine

Mycophenolattotal

Phenobarbital

Phenytoin

Syphilis TreponemaPallidum Antigen

CreatinineJaffe

Lactate Glucose

N-Acetylprocainamide

Primidon Procain-amide

Thyroxine(T4)* Acid Phosphatase

Alkaline Phosphatase

CRPCRP hs

Cystatin C

Ferritin

Complement C3c

Complement C4

Albumin T

Pancreatic EDDPalpha-Amylase

α-Amylase

CK-MB

CK

Digoxin

Digitoxin

Salicylate

LSD

ValproicAcid

Methaqualone

EDDPMethadone

LidocaineTheophylline

Tobramycin

Cocaine

Ethanol

Propoxyphene

Vancomycin

Cannabinoids

Oxycodone

Benzo-diazepines

SerumBenzo-diazepines

Amphetamines

Barbiturates

SerumBarbiturates

CHEAcetylCHE

DibucaineLDH

HBDH

GLDH

AST +/- PYP

ALT +/- PYPLipasePhencyclidine

Opiates

GGT

Kappa Light ChainFree Kappa Light Chain

Lambda Light ChainFree Lambda Light Chain

Lipoprotein (a)

HbA1chemolysate

HbA1c wholeblood

Haptoglobin

RF

Pre-albumin

Trans-ferrine

SolubleTransferrinReceptor

Myoglobin

Magnesium

Total Protein Urine / CSF

Total Protein

UREA/BUN

Pre Acid

Homocysteine

Phosphorus

IgMIgGCSF

IgG

Potassium

Chloride

LithiumCalcium

Iron UIBC

Syphilis RapidPlasma Reagin

Sodium

IgA

Existing product

In development

Others• distribution in limited

countries

DATISESubstrates

ProteinsEnzymesTDM

IgMCSF

IgGurine

IgACSF

Roche kemiinstrumentAnalytisk sensitivitet

mmol/Lmg/L

GLUKOS

μg/Lμmol/L

KREATININ

mol/Lg/L

ALBUMIN

Roche kemiinstrumentcobas c111 och c311

Roche kemiinstrumentcobas c501/502 och c701/702

Roche kemiinstrumentProvpipettering

Roche kemiinstrumentProvpipettering

Roche kemiinstrumentReagenspipettering

Roche kemiinstrumentReagenspipettering

Roche kemiinstrumentReaktionsområde

Roche kemiinstrumentReaktionsområde

9 September 2016 page 17 © 2009 Roche

0 5 10

Reagenspipettering 1

Prov-pipettering

1st mixning

Reagenspipettering 2

Reagenspipettering 3

2nd mixning 3rd mixing

11

1

3570

Blank mätning

Tid/ Min

1) Prov 2) Reagens R1(0 min)3 sekunder

3) Reagens R2 (1,5 min)90 sekunder

4) Reagens R3(5 min)300 sekunder

Roche kemiinstrumentFotometri

Roche kemiinstrumentUltraljudsmixrar

9 September 2016 page 19 © 2009 Roche

Roche kemiinstrumentKolorimetri och turbidimetri

9 September 2016 page 20 © 2009 Roche

Roche kemiinstrumentFotometri

Roche kemiinstrumentFotometri, bikromatisk analys

Roche kemiinstrumentISE

Roche kemiinstrumentISE

Doing now what patients need next