RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in...

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RNA polymerase σ α 2 ββ’ Core enzyme σ promot er DNA α 2 ββ’ α 2 ββ’ Transcription in Proca ryotes

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Page 1: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

RNA polymerase

σ

α2ββ’ Core enzyme

σ promoter

DNA

α2ββ’ α2ββ’

Transcription in Procaryotes

Page 2: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.
Page 3: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

RNA polymerase

σ

α2ββ’ Core enzyme

σ promoter

DNA

α2ββ’ α2ββ’

σ factor ： rpoD

rpoH

rpoN…等

Regulation

Page 4: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.
Page 5: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

Figure 9.03

Page 6: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

Heat shock proteins

Page 7: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

lac operon

Page 8: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.
Page 9: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.
Page 10: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

araBAD operon

Page 11: RNA polymerase σ α 2 ββ’ Core enzyme σ promoter DNA α 2 ββ’ Transcription in Procaryotes.

What do we mean by the 5´ end of a gene?

• Since DNA has two antiparallel strands it has no actual polarity.

• mRNA does have polarity since it is single stranded. The 5´ end refers to the mRNA molecule and since it is copied from the DNA by standard base pairing the 5´ end of a gene is the 3´ end of the template strand.


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