Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as...

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biolegend.com [email protected] 159 Rat Immunology Rat Immunology Rat CDs and Related Molecules CD2 160 CD3 160 CD4 (domain 1) 160 CD4 (domain 2) 161 CD5 161 CD6 161 CD8a 162 CD8b 162 CD11b/c (Integrin α M / α X ) 163 CD25 (IL-2Rα) 163 CD26 163 CD28 163 CD29 (Integrin β 1 ) 164 CD43 164 CD44H (Pgp-1, H-CAM) 164 CD45 (LCA) 165 CD45RA 165 CD45RC 165 CD47 166 CD48 166 CD49d (Integrin α 4 ) 166 CD49e (Integrin α 5 ) 166 CD53 167 CD54 (ICAM-1) 167 CD61 (Integrin β 3 ) 167 CD62L (L-Selectin) 168 CD71 (Transferrin Receptor) 168 CD80 (B7-1) 168 CD81 (TAPA-1) 168 CD86 (B7-2) 169 CD90 (Thy-1)  169 CD103 (αE2 Integrin) 169 CD106 (VCAM-1) 170 CD126 (IL-6Rα) 170 CD134 (OX-40) 170 CD147 171 CD152 171 CD161 171 CD172a (SIRPα) 172 CD178 (FasL/CD95L) 172 CD200 172 CD200 Receptor 173 CD252 (OX-40 Ligand) 173 CD278 (ICOS) 173 αE2 Integrin (CD103) 169 Endothelium 173 Fas Ligand (CD95L, CD178) 172 FOXP3 174 FOXP3 Flow Kit 174 ICAM-1 (CD54) 167 ICOS (CD278) 173 IgM 174 IL-2Rα (CD25) 163 IL-6Rα (CD126) 170 Integrin α 4 (CD49d) 166 Integrin α 5 (CD49e) 166 Integrin α M / α X (CD11b/c) 163 Integrin β 1 (CD29) 164 Integrin β 3 (CD61) 167 L-Selectin (CD62L) 168 Myeloid Lineage 175 OX-40 Ligand (CD252) 173 PCNA 175 RT1A 175 RT1B 175 RT1D 176 Thy-1 (CD90) 169 VCAM-1 (CD106) 170 Rat T Cell Receptors (TCRs) TCR α/β 176 TCR γ/δ 177 TCR Vβ82/84 177 Rat Adhesion Molecules H-CAM (CD44H, Pgp-1) 164 Integrin α 4 (CD49d) 166 Integrin α 5 (CD49e) 166 Integrin α M / α X (CD11b/c) 163 Integrin β 1 (CD29) 164 Integrin β 3 (CD61) 167 ICAM-1 (CD54) 167 L-Selectin (CD62L) 168 VCAM-1 (CD106) 170 Dog CD CD34 177

Transcript of Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as...

Page 1: Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as LFA-2, is a member of the immunoglobulin ... $300 Alexa Fluor® 647 203408 100 μg $225

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Rat Immunology

Rat CDs and Related MoleculesCD2 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 160CD3 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 160CD4 (domain 1)� � � � � � � � � � � � � � � � � � � � � � � 160CD4 (domain 2)� � � � � � � � � � � � � � � � � � � � � � � 161CD5 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 161CD6 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 161CD8a� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 162CD8b � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 162CD11b/c (Integrin αM/αX) � � � � � � � � � � � � � � 163CD25 (IL-2Rα) � � � � � � � � � � � � � � � � � � � � � � � � � 163CD26� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 163CD28� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 163CD29 (Integrin β1) � � � � � � � � � � � � � � � � � � � � 164CD43� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 164CD44H (Pgp-1, H-CAM) � � � � � � � � � � � � � � � 164CD45 (LCA) � � � � � � � � � � � � � � � � � � � � � � � � � � � 165CD45RA � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 165CD45RC � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 165CD47� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 166CD48� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 166CD49d (Integrin α4) � � � � � � � � � � � � � � � � � � � 166CD49e (Integrin α5) � � � � � � � � � � � � � � � � � � � 166CD53� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 167CD54 (ICAM-1)� � � � � � � � � � � � � � � � � � � � � � � � 167CD61 (Integrin β3) � � � � � � � � � � � � � � � � � � � � 167CD62L (L-Selectin) � � � � � � � � � � � � � � � � � � � � 168CD71 (Transferrin Receptor)� � � � � � � � � � � 168CD80 (B7-1)� � � � � � � � � � � � � � � � � � � � � � � � � � � 168CD81 (TAPA-1) � � � � � � � � � � � � � � � � � � � � � � � � 168

CD86 (B7-2)� � � � � � � � � � � � � � � � � � � � � � � � � � � 169CD90 (Thy-1)  � � � � � � � � � � � � � � � � � � � � � � � � � 169CD103 (αE2 Integrin) � � � � � � � � � � � � � � � � 169CD106 (VCAM-1) � � � � � � � � � � � � � � � � � � � � � � 170CD126 (IL-6Rα)� � � � � � � � � � � � � � � � � � � � � � � � 170CD134 (OX-40)� � � � � � � � � � � � � � � � � � � � � � � � 170CD147� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 171CD152� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 171CD161� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 171CD172a (SIRPα) � � � � � � � � � � � � � � � � � � � � � � � 172CD178 (FasL/CD95L) � � � � � � � � � � � � � � � � � � 172CD200 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 172CD200 Receptor � � � � � � � � � � � � � � � � � � � � � � 173CD252 (OX-40 Ligand) � � � � � � � � � � � � � � � � 173CD278 (ICOS) � � � � � � � � � � � � � � � � � � � � � � � � � 173αE2 Integrin (CD103)� � � � � � � � � � � � � � � � � � 169Endothelium � � � � � � � � � � � � � � � � � � � � � � � � � 173Fas Ligand (CD95L, CD178) � � � � � � � � � � � � 172FOXP3 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 174FOXP3 Flow Kit � � � � � � � � � � � � � � � � � � � � � � � 174ICAM-1 (CD54)� � � � � � � � � � � � � � � � � � � � � � � � 167ICOS (CD278) � � � � � � � � � � � � � � � � � � � � � � � � � 173IgM � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 174IL-2Rα (CD25) � � � � � � � � � � � � � � � � � � � � � � � � � 163IL-6Rα (CD126)� � � � � � � � � � � � � � � � � � � � � � � � 170Integrin α4 (CD49d) � � � � � � � � � � � � � � � � � � � 166Integrin α5 (CD49e) � � � � � � � � � � � � � � � � � � � 166Integrin αM/αX (CD11b/c) � � � � � � � � � � � � � � 163Integrin β1 (CD29)� � � � � � � � � � � � � � � � � � � � � 164Integrin β3 (CD61)� � � � � � � � � � � � � � � � � � � � � 167

L-Selectin (CD62L) � � � � � � � � � � � � � � � � � � � � 168Myeloid Lineage � � � � � � � � � � � � � � � � � � � � � 175OX-40 Ligand (CD252) � � � � � � � � � � � � � � � � 173PCNA � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 175RT1A � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 175RT1B � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 175RT1D � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 176Thy-1 (CD90) � � � � � � � � � � � � � � � � � � � � � � � � � 169VCAM-1 (CD106) � � � � � � � � � � � � � � � � � � � � � � 170

Rat T Cell Receptors (TCRs)TCR α/β � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 176TCR γ/δ� � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 177TCR Vβ8�2/8�4 � � � � � � � � � � � � � � � � � � � � � � � � 177

Rat Adhesion MoleculesH-CAM (CD44H, Pgp-1) � � � � � � � � � � � � � � � 164Integrin α4 (CD49d) � � � � � � � � � � � � � � � � � � � 166Integrin α5 (CD49e) � � � � � � � � � � � � � � � � � � � 166Integrin αM/αX (CD11b/c) � � � � � � � � � � � � � � 163Integrin β1 (CD29)� � � � � � � � � � � � � � � � � � � � � 164Integrin β3 (CD61)� � � � � � � � � � � � � � � � � � � � � 167ICAM-1 (CD54)� � � � � � � � � � � � � � � � � � � � � � � � 167L-Selectin (CD62L) � � � � � � � � � � � � � � � � � � � � 168VCAM-1 (CD106) � � � � � � � � � � � � � � � � � � � � � � 170

Dog CDCD34 � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � � 177

Page 2: Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as LFA-2, is a member of the immunoglobulin ... $300 Alexa Fluor® 647 203408 100 μg $225

160 Toll Free 1.877.BIOLEGEND (246.5343)

Rat I

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Rat CD2Other Names: LFA-2, T11

CD2, also known as LFA-2, is a member of the immunoglobulin gene family with an approximate molecular weight of 45 kD� It is expressed on thymocytes, peripheral T cells, dendritic epider-mal T cells, macrophages in the spleen, and NK cells� CD2 can as-sociate with the T cell receptor and participate in T cell activation through adhesion and signal transduction� CD2 binds to CD48, CD58, and CD59�

Clone: OX-34 Isotype: Mouse IgG2a, κThe OX-34 antibody blocks CD48 binding to CD2, partially blocks T cell activation, and inhibits the mixed lymphocyte reaction in vitro� The OX-34 antibody has also been used to deplete periph-eral T cells and prevent allograft rejection in vivo�

Immunogen: Rat T cell blasts from mixed lymphocyte reactions

Lou rat splenocytes stained with OX-34 FITC

Applications: FC—Quality tested IHC (Frozen/Paraffin), IP—Re-ported in the literature

Application References: 1� Williams, A� F�, et al. 1987� J. Exp. Med. 165:368� ✦ 2� Jefferies, W� A�, et al. 1985� J. Exp. Med. 162:117� ✦ 3� Hirahara, H�, et al. 1995� Transplantation 59:85�

Clone OX-34 Purified201301100 μg

$105

FITC201303100 μg

$130

PE20130550 μg$115

Rat CD3Other Names: T3, T cell antigen receptor complex

CD3 is a complex composed of δ, γ, ε, and ζ chains� They are 20–25 kD members of the immunoglobulin superfamily and as-sociated with the T cell receptor (TCR)� CD3 is expressed on dif-ferentiated thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells� CD3 is involved in antigen recognition, signal transduction, and T cell activation�

Clone: 1F4 Isotype: Mouse IgM, κImmunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore

Lou rat splenocytes stained with 1F4 FITC

Applications: FC—Quality tested IHC (Frozen), IF—Reported in the literature

Application References: 1� Tanaka, T�, et al. 1989� J. Immunol. 142:2791� (Activ, IHC, IP) ✦ 2� Nicholls, M�R�, et al. 1993� Transplantation 55:459� (Block) ✦ 3� Elbe, A�, et al. 1993� J. Invest. Dermatol� 102:74� (IF) ✦ 4� Baba, T�, et al. 2006� Blood 107:2004� (IHC) ✦ 5� Li, X�, et al. 2009� J. Immunol. 183:3955� (FC)

Clone 1F4 Purified201401100 μg

$85

LEAF™201410500 μg

$290

FITC201403100 μg

$130

Alexa Fluor® 48820140525 μg$85

201406100 μg

$195

Alexa Fluor® 647201408100 μg

$195

Rat CD4 (domain 1)Other Names: T4, L3T4, W3/25

CD4, also known as T4, is a 55 kD glycoprotein member of the im-munoglobulin superfamily and is expressed on the majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)� CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation�

Clone: W3/25 Isotype: Mouse IgG1, κThe W3/25 antibody has been shown to bind the same CD4 epit-ope as the OX-38 antibody and a different epitope than the OX-35 antibody� The W3/25 antibody has been shown to inhibit IL-2 pro-duction by T helper cells and to prevent autoimmune T cell trans-fer in an MBP induced EAE model in vivo�

Immunogen: Rat thymocyte membrane glycoproteins

Lou rat splenocytes stained with W3/25 PE

Applications: FC—Quality tested IHC (Frozen)—Reported in the literature

Application References: 1� Whiteland, J�L�, et al. 1995� J. Histochem. Cytochem. 43:313� (IHC) ✦ 2� Shioji, K�, et al. 2001� Circulation Res. 89:540� (IHC) ✦ 3� Mannie, M�D�, et al. 1993� J. Immunol. 151:7293� ✦ 4� Kurtz, CC�, et al. 2007� Dev. Comp. Immunol. 31:415�

Clone W3/25 Purified201501100 μg

$105

FITC201505100 μg

$130

PE20150750 μg$115

PE/Cy720151525 μg$125

201516100 μg

$275

APC20150950 μg$150

APC/Cy720151725 μg$140

201518100 μg

$295

Alexa Fluor® 48820151125 μg$85

Alexa Fluor® 64720151325 μg$85

201514100 μg

$195

LEAF™: Low Endotoxin, Azide-Free

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Clone: OX-38 Isotype: Mouse IgG2a, κThe OX-38 antibody has been shown to bind the same CD4 epit-ope as the W3/25 antibody, and a different epitope than the OX-35 antibody�

Immunogen: Rat thymocyte glycoproteins

LOU rat splenocytes stained with OX-38 FITC

Applications: FC—Quality tested IHC (Frozen), Block—Reported in the literature

Application References: 1� Pettigrew, G�J�, et al. 1998� J. Immunol. 161: 1292� (Block)

Clone OX-38 LEAF™203410500 μg

$300

FITC203405100 μg

$130

203406500 μg

$275

PE20341150 μg$115

203412200 μg

$300

Alexa Fluor® 647203408100 μg$225

Rat CD4 (domain 2)

Clone: OX-35 Isotype: Mouse IgG1, κThe OX-35 antibody has been shown to bind a different CD4 epi-tope than the W3/25 and OX-38 antibodies�

Immunogen: MLR generated rat T helper lymphocytes

LOU rat splenocytes stained with OX-35 Alexa Fluor® 488

Applications: FC—Quality tested IHC—Reported in the literature

Application References: 1� May E�, et al. 2003� J Immunol. 170: 1099� ✦ 2� Hansson, AS�, et al. 1999� J Clin Invest. 104: 589� (IHC)

Clone OX-35 Purified203301100 μg

$105

FITC203305100 μg

$130

PE20330750 μg$115

203308200 μg

$295

Alexa Fluor® 488203310100 μg$225

Alexa Fluor® 647203312100 μg$225

Rat CD5Other Names: Ly-1, Tp67, T1, Leu-1

CD5, a 67 kD glycoprotein, is a member of the scavenger recep-tor superfamily� It is expressed on thymocytes, T cells, and a sub-set of B cells� CD5 has been reported to associate with the T cell receptor, the B cell receptor and many accessory proteins (CD21, CD4, CD8)� In some cases, CD5 can augment signaling, while in

other cases, it appears to confer an inhibitory signal (depending on the cell type and differentiation state)�

Clone: R1-3B3 Isotype: Mouse IgG2a, κImmunogen: Rat thymocytes

Lou rat splenocytes stained with R1-3B3 FITC

Applications: FC—Quality tested

Application References: 1� Matsuura, A�, et al. 1984� J. Immunol. 132: 316�

Clone R1-3B3 FITC

201603100 μg

$130

Clone: OX-19 Isotype: Mouse IgG1, κImmunogen: Rat thymocyte lentil lectin-binding glycoproteins

LOU rat splenocytes stained with OX-19 biotin

Applications: FC—Quality tested IHC (Frozen)—Reported in the literature

Application References: 1� Dallman, M�J�, et al. 1984� Eur. J. Immunol. 14:260� ✦ 2� Vemeer, L�A�, et al. 1994� Eur. J. Immunol. 24:585�

Clone OX-19 Purified203501100 μg

$105

Biotin203503100 μg

$130

FITC203505100 μg

$130

Rat CD6Other Name: T12

Rat CD6 is a membrane molecule of 95-120 kD, expressed primar-ily on all the T lymphocytes of alpha/beta or gamma/delta TCR� Rat NK cells express low levels of CD6� In immunoprecipitation studies, CD6 was found to co-precipitate with highly phorsphor-ylated CD5, and other protein kinases, such as Lck, Fyn, ZAP70, and Itk�

Clone: OX-52 Isotype: Mouse IgG2a, κImmunogen: Rat thoracic-duct leukocytes

ContinuesLEAF™: Low Endotoxin, Azide-Free

Page 4: Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as LFA-2, is a member of the immunoglobulin ... $300 Alexa Fluor® 647 203408 100 μg $225

162 Toll Free 1.877.BIOLEGEND (246.5343)

Rat I

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ogy

LOU rat splenocytes stained with OX-52 Alexa Fluor® 647

Applications: FC—Quality tested IP, IHC (Frozen)—Reported in the literature

Application References: 1� Robinson, A�P�, et al. 1986� Immunology 57: 527�

Clone OX-52 Purified203601100 μg

$95

Alexa Fluor® 647203608100 μg$225

Rat CD8aOther Names: CD8, CD8α, T8, Lyt2, Ly-2, Leu-8

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α� CD8a is a member of the immunoglobulin superfamily ex-pressed on most thymocytes, a subset of mature T cells, most NK cells, macrophages, and some activated (not resting) CD4+ T cells� CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lym-phocytes almost exclusively express the CD8 αβ heterodimer� In-testinal intraepithelial lymphocytes express CD8a without CD8b� CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells� CD8 partici-pates in T cell activation through its association with the T cell re-ceptor complex and protein tyrosine kinase lck (p56lck)�

Clone: OX-8 Isotype: Mouse IgG1, κThe OX-8 antibody has been reported to partially block T cell re-sponses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation�

Immunogen: High-molecular-weight rat thymocyte glycoproteins

Reactivity: Rat

Lou rat splenocytes stained with OX-8 FITC

Applications: FC—Quality tested IHC (Frozen/Paraffin/Zinc), IP, WB, FA—Reported in the literature

Application References: 1� Barclay, A�N�, 1981� Immunology 42:593� (IHC) ✦ 2� Wallgren, A�C�, et al. 1995� Transplantation 60:594� (IHC) ✦ 3� Torres-Nagel, N�, et al. 1992� Eur. J. Immunol. 22:2841� (IP, WB) ✦ 4� Mason, D�W�, et al. 1983� Immunol. Rev. 74:57�

✦ 5� Hirji, N�, et al. 1997� J. Immunol. 158:1833� (FA) ✦ 6� Mitnacht, R�, et al. 1998. J. Immunol. 160:700� (WB)

Clone OX-8 Purified201701100 μg

$105

LEAF™201714500 μg

$300

FITC201703100 μg

$130

PE20170550 μg$115

201706200 μg

$300

Alexa Fluor® 647201710100 μg$225

PerCP201712100 μg$250

Clone: G28 Isotype: Mouse IgG2a, κImmunogen: CD8+ Wistar rat splenic T cell hybridomas

Lou rat splenocytes stained with G28 PE

Applications: FC—Quality tested IP, IHC (Frozen)—Reported in the literature

Application References: 1� Torres-Nagel, N� et al. 1992� Eur. J. Immunol. 22: 2841� (IP)

Clone G28 Purified200602500 μg

$210

Biotin20060350 μg

$70

PE20060750 μg

$95

200608200 μg

$265

APC20060925 μg$80

200610100 μg$260

Rat CD8bOther Names: CD8, CD8β, Lyt3

CD8b antigen is also known as T8, Lyt3, and CD8β� It is a member of the immunoglobulin superfamily expressed on most thymo-cytes, some mature T cell subsets, and macrophages� CD8b forms heterodimers with the CD8α chain (CD8a)� CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-pre-senting cells or epithelial cells� CD8 participates in T cell activa-tion through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck)�

Clone: 341 Isotype: Mouse IgG1, κImmunogen: CD8+ Wistar rat splenic T cell hybridomas

Lou rat splenocytes stained with 341 FITC

Applications: FC—Quality tested IP, WB, IHC (Frozen/Paraffin)—Reported in the literature

Application References: 1� Torres-Nagel, N�, et al. 1992� Eur. J. Immunol. 22:2841� (IP, IHC, WB) ✦ 2� Kuhnlein, P�, et al. 1994� J. Immunol. 153:979� (IHC) ✦ 3� Hirji, N�, et al. 1997� J. Immunol. 158:1833� ✦ 4� Hirji, N�, et al. 1998� J. Immunol. 160:6004�

Clone 341 Purified200702500 μg

$150

FITC20070550 μg$85

Alexa Fluor® 488200710100 μg

$195

Alexa Fluor® 647200712100 μg

$195

Rat CD6 continued

LEAF™: Low Endotoxin, Azide-Free

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Rat CD11b/c (Integrin αM/αX)Other Names: Rat C3bi receptor, Rat CR3 complement receptor

The CR3 complement (C3bi) receptor is expressed on monocytes, granulocytes, macrophages, dendritic cells, NK cells, and a sub-set of lymphocytes�

Clone: OX-42 Isotype: Mouse IgG2a, κThis antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains)� The OX-42 antibody precipitates three polypeptides with apparent molecu-lar weights of 160, 103, and 95 kD, respectively� This antibody has been shown to block the formation of complement-mediated ro-settes and leukocyte migration�

Immunogen: Rat peritoneal macrophages

Lou rat splenocytes stained with OX-42 PE

Applications: FC—Quality tested IHC (Frozen), IF, IP—Reported in the literature

Application References: 1� Whiteland, J�L�, et al. 1995� J. Histochem. Cytochem. 43:313� (IHC) ✦ 2� Milligan,

C�E�, et al. 1991� J. Comp. Neurol� 314:125� (IHC) ✦ 3� Robinson, A�P�, et al. 1986� Immunology 57:239� (Block) ✦ 4� Issekutz, S�E�, et al. 1992� Immunology 76:655� (Block) ✦ 5� Muehlbauer, SM�, et al. Am� J Pathol� 177:735� (FC)

Clone OX-42 Purified201801100 μg

$105

Biotin201803100 μg

$130

FITC201805100 μg

$130

PE20180750 μg$115

APC20180950 μg$150

Alexa Fluor® 488201812100 μg

$195

Alexa Fluor® 64720181325 μg$85

201814100 μg

$195

Rat CD25 (IL-2Rα)Other Names: IL-2 Receptor chain α, Low affinity IL-2 receptor chain, Tac, p55, Ly-43

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain� It is broadly expressed on activated T and B cells, a subset of thy-mic and splenic dendritic cells, and intestinal epithelial cells in the rat� IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion� IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ�

Clone: OX-39 Isotype: Mouse IgG1, κThe OX-39 antibody weakly inhibits IL-2 binding to the IL-2 recep-tor and has been shown to block some IL-2 mediated responses in vitro�

Immunogen: Rat T cell blasts from mixed lymphocyte reactions

Con A-stimulated (3 days) Lou rat splenocytes stained

with purified OX-39, followed by FITC anti-mouse IgG

Applications: FC—Quality tested IHC (Frozen), IP—Reported in the literature

Application References: 1� Peterson, D�J�, et al. 1987� Mol. Immunol. 24:1281� (IHC, IP, Block) ✦ 2� Tellides, G�, et al. 1987� Transplant Proc. 19:4231� (Block) ✦ 3� Digness, A�U�, et al. 1996� Exp. Cell Res. 225:422� (Block)

Clone OX-39 Purified202101100 μg

$95

Biotin202111100 μg

$130

FITC202103100 μg

$130

PE20210550 μg$115

Alexa Fluor® 488202108100 μg

$195

Rat CD26Other Names: DPP IV ectoenzyme, gp110

CD26 is a 110 kD type II membrane protein also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV)� It is a mem-ber of the peptidase and ectoenzyme family� CD26 is expressed on membrane thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, macrophages, epithelial cells, and en-dothelial cells� Rat CD26 (unlike in humans) does not bind ADA, but may interact with extracellular matrix proteins such as fibro-nectin or collagen� CD26 plays an integral role as a costimulatory molecule in T cell activation�

Clone: OX-61 Isotype: Mouse IgG2a, κImmunogen: 110 kD membrane glycoprotein of rat

LOU rat splenocytes stained with OX-61 PE

Applications: FC—Quality tested IHC (Frozen), WB, IP—Reported in the literature

Application References: 1� Cohen, D�, et al. 2004� Proc Natl Acad Sci U S A 101: 13792�

Clone OX-61 Purified203801100 μg

$95

PE203806100 μg

$195

Rat CD28Other Names: Tp44, T44

CD28 is a 44 kD glycoprotein also known as Tp44 or T44� It is a member of the immunoglobulin superfamily and is expressed on thymocytes, α/βTCR+ T cells, most γ/δTCR+ T cells, and an NK cell subset� During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation�

Continues

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Clone: JJ319 Isotype: Mouse IgG1, κImmunogen: Rat CD28-transfected BALB/c mouse A2OJ cell line

Lou rat splenocytes stained with LEAF™

purified JJ319, followed by FITC anti-mouse IgG

Applications: FC—Quality tested Costim, IP—Reported in the literature

Application References: 1� Tacke, M�, et al. 1995� J. Immunol. 154:5121� (Costim) ✦ 2� Tacke, M�, et al. 1997� Eur. J. Immunol. 27:239� (Costim)

Clone JJ319 Purified

20090150 μg$60

200902500 μg

$215

FITC20090350 μg$80

APC200908100 μg$260

Rat CD29 (Integrin β1)Other Names: VLA-β chain, Integrin β1 chain, GPIIa

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa� It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells� In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively� It plays an important role in cell-cell or cell-matrix interaction�

Clone: HMβ1-1 Isotype: Armenian Hamster IgG

The HMβ1-1 antibody reacts with both mouse and rat CD29�

Immunogen: Purified mouse VLA-4 (α4β1, CD49d/CD29)

Reactivity: Mouse, Rat

Lou rat bone marrow cells stained with biotinylated

HMβ1-1, followed by Sav-PE

Applications: FC—Quality tested IP, Block, IHC (Frozen), IF—Re-ported in the literature

Application References: 1� Noto, K�, et al. 1995� Int. Immunol. 7: 835� ✦ 2� Ridger, V�C�, et al. 2001� J. Immunol. 166: 3484� ✦ 3� Jia, W�, et al. 2005� Blood 106: 3854� ✦ 4� Economopoulou, M�, et al. 2005� Blood 106: 3831� ✦ 5� Lawson, B� R�, et al. 2007� J. Immunol. 178: 5366�

Clone HMβ1-1 Purified

10220150 μg

$70

102202500 μg

$220

LEAF™10220950 μg$90

102210500 μg

$300

Biotin10220350 μg

$75

102204500 μg

$250

FITC10220550 μg$85

102206500 μg

$280

PE10220750 μg$105

102208200 μg

$290

PE/Cy510221925 μg

$95

102220100 μg

$270

PE/Cy710222125 μg

$95

102222100 μg

$270

APC10221525 μg

$95

102216100 μg

$270

APC/Cy710222525 μg$105

102226100 μg

$285

Alexa Fluor® 48810221125 μg$85

102212100 μg

$195

Alexa Fluor® 64710221325 μg$85

102214100 μg

$195

Alexa Fluor® 700102218100 μg

$195

Pacific Blue™102224100 μg

$195

Rat CD43Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Si-aloglycoprotein, and W3/13, is a large single chain of type I trans-membrane glycoprotein with abundant O-glycosylation and si-alylation sites� It has been reported that CD43 binds to CD54 and Siglec-1� CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and in-duction of apoptosis of T cells and hematopoietic progenitors�

Clone: W3/13 Isotype: Mouse IgG1, κImmunogen: Rat thymocyte membrane glycoproteins

LOU rat splenocytes stained with W3/13 Alexa Fluor® 647

Applications: FC—Quality tested IHC (Frozen/Paraffin)—Reported in the literature

Application References: 1� Barclay, A�N�, 1981� Immunology 42: 593� (IHC) ✦ 2� Brown, W�R�A� et al. 1981� Nature 289: 456� ✦ 3� Forbes, R�C�, et al. 1983� Transplantation 36: 681� ✦ 4� Jung, S�, et al. 1994� Immunology 83: 545� (IHC)

Clone W3/13 Purified202801100 μg

$105

Biotin202803100 μg

$130

PE202812100 μg

$175

Alexa Fluor® 647202810100 μg$225

Rat CD44 (Pgp-1, H-CAM)Other Names: Hermes, Pgp-1, HUTCH-1, ECMR III, gp85, Ly-24, CD44H, CD44s

CD44 is a 80–95 kD glycoprotein, also known as CD44H or CD44s, Hermes, Pgp1, H-CAM, or HUTCH� It is expressed on all leukocytes (except a subset of B cells), endothelial cells, hepatocytes, and mesenchymal cells� The expression level of CD44 on lymphocytes is upregulated on T- and B-cell blasts� CD44 is an adhesion mole-cule involved in leukocyte attachment to, and rolling on, endothe-lial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation� Hyaluronate, type I collagen, and fibronectin are the ligands of CD44�

Clone: OX-49 Isotype: Mouse IgG2a, κImmunogen: Rat T blasts from mixed lymphocyte reactions

Rat CD28 continued

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LOU rat splenocytes stained with OX-49 FITC

Applications: FC—Quality tested IHC (Frozen/Paraffin), IP—Re-ported in the literature

Application References: 1� Paterson, D�J�, et al. 1987� Mol. Immunol. 24: 1281�

Clone OX-49 Purified

20390125 μg

$55

203902100 μg

$105

FITC203906100 μg

$130

Alexa Fluor® 647203908100 μg$225

Rat CD45 (LCA)Other Names: Leukocyte common antigen, T200, B220, Ly-5

CD45 is a 180–220 kD protein also known as leukocyte common antigen (LCA)� It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracel-lular domain and glycosylation� CD45 is expressed on all hemato-poietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation� CD45 functions in signal transduction through T and B cell antigen re-ceptors� CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4�

Clone: OX-1 Isotype: Mouse IgG1, κThe OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro�

Immunogen: Enriched glycoprotein fraction from Wistar rat thymocytes

Lou rat splenocytes stained with OX-1 FITC

Applications: FC—Quality tested IF, IP, IHC (Frozen/Paraffin)—Re-ported in the literature

Application References: 1� Giezeman-Smits, K�M�, et al. 1999� J. Immunol. 163:71� (IP) ✦ 2� Elbe, A�, et al. 1994� J. Invest. Dermatol. 102:74� (IF) ✦ 3� Kouwenhoven, E�, et al. 2001� Kidney Int. 59:1142� (IHC) ✦ 4� Martin, A�, et al. 1995 Clin. Exp. Immunol.

22:283� (IHC) ✦ 5� Sayegh, M�H�, et al. 1995 J. Exp. Med. 181:186� (IHC) ✦ 6� Morioka, Y�, et al. 2000� Kidney Int. 60:2192� (IHC)

Clone OX-1 Purified202201100 μg

$105

Biotin202203100 μg

$130

FITC202205100 μg

$130

PE20220750 μg$115

PE/Cy720221325 μg$125

202214100 μg

$275

APC/Cy7202216100 μg

$295

Alexa Fluor® 488202210100 μg

$195

Alexa Fluor® 64720221125 μg$85

202212100 μg

$195

Alexa Fluor® 700202218100 μg

$205

PerCP/Cy5.5202220100 μg

$295

Rat CD45RAOther Names: Leukocyte common antigen, high molecular weight form

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular do-main and glycosylation� CD45 is expressed on all hematopoietic cells except erythrocytes and platelets� CD45RA is one of the CD45 isoforms with the molecular weight of 200–220 kD� It is expressed almost exclusively on B cells� CD45 functions in signal transduc-tion through T and B cell antigen receptors� CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4�

Clone: OX-33 Isotype: Mouse IgG1, κImmunogen: Leukocyte common antigen purified from rat splenocytes

Lou rat splenocytes stained with biotinylated OX-33,

followed by Sav-PE

Applications: FC—Quality tested IHC (Frozen)—Reported in the literature

Application References: 1� Smith, T�, et al. 2000� Nature Med. 6:62� (IHC)

Clone OX-33 Purified202301100 μg

$105

Biotin202303100 μg

$130

FITC202305100 μg

$130

PE20230750 μg$115

APC20231325 μg$80

202314100 μg

$185

Alexa Fluor® 488202310100 μg

$195

Rat CD45RC

CD45RC is a 200–220 kD single chain type I membrane glycopro-tein� It is an exon 5 splice variant (exon C) of the tyrosine phos-phatase CD45� The CD45RC isoform is expressed on B cells, the majority of CD8+ T cells, and a subset of CD4+ T cells but not on myeloid cells� CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes� CD45 has been reported to be associated with several other cell surface antigens, including CD1, CD2, CD3, and CD4� CD45 is involved in signaling lymphocytes activation�

Clone: OX-22 Isotype: Mouse IgG1, κImmunogen: PHA-activated rat lymph node cells

ContinuesLEAF™: Low Endotoxin, Azide-Free

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LOU rat splenocytes stained with OX-22 PE

Applications: FC—Quality tested IHC (Frozen/Paraffin)—Reported in the literature

Application References: 1� Woollett, G�R�, et al. 1985� Eur. J. Immunol. 15: 168� ✦ 2� Spickett, G�P�, et al. 1983� J. Exp. Med. 158: 795� (IHC) ✦ 3� Mason, D�W�, et al. 1983� Immunol. Rev� 74: 57� (IHC)

Clone OX-22 Purified204002500 μg

$260

PE20400750 μg$115

Alexa Fluor® 647204010100 μg$225

Rat CD47Other Names: gp42, Integrin-associated protein, IAP, Neurophilin

CD47, also known as gp42, integrin-associated protein (IAP), and neurophilin, is a 42–52 kD member of the immunoglobulin super-family� CD47 expression is widely distributed in hematopoietic and non-hematopoietic tissues, such as leukocytes, macrophages, epithelial, and endothelial cells� CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61� CD47 is also the ligand of signal regulatory protein SIRP, an Ig superfamily in-hibitory receptor expressed mainly on myeloid and neuronal cells�

Clone: OX-101 Isotype: Mouse IgG1, κImmunogen: Rat thymocytes

LOU rat splenocytes stained with OX-101 Alexa Fluor® 647

Applications: FC—Quality tested

Application References: 1� de Vries, H�E�, et al. 2002� J. Immunol. 168 : 5832�

Clone OX-101 Purified204101100 μg

$105

PE204108100 μg

$195

Alexa Fluor® 647204110100 μg$225

Rat CD48Other Names: BCM1, OX-45

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), or OX-45 (rat)� It is a member of the Ig superfamily, expressed on T and B cells, and monocytes/macrophages� It plays a role in adhesion and T cell recognition� The primary ligands for CD48 are CD2, CD244 (2B4), or 2B4-related protein (2B4R)�

LOU rat splenocytes stained with OX-45 PE

Clone: OX-45 Isotype: Mouse IgG1, κThe OX-45 mAb inhibits an-tigen-induced T-lymphocyte proliferation in vitro by potenti-ating suppressive effects of ac-cessory cells�

Immunogen: Rat T cell blasts

Applications: FC—Quality tested IHC (Frozen)—Reported in the literature

Application References: 1� Arvieux, J�, et al. 1986� Immunology 58: 337�

Clone OX-45 Purified204201100 μg

$90

PE204206100 μg

$195

Rat CD49d (Integrin α4)Other Names: Integrin α4 chain, VLA-4 α chain

CD49d is a 150 kD glycoprotein also known as integrin α4 or VLA-4 α chain� It is expressed as a heterodimer with either of two β chains, β1 or β7� The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymo-cytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes� The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by in-teracting with the ligands CD106 (VCAM-1), MAdCAM-1, and fi-bronectin�

Clone: MRα4-1 Isotype: Mouse IgG2a, κImmunogen: RBL-2H3 rat basophilic leukemia cell line

Applications: FC—Quality tested IP, Activ, Block—Reported in the literature

Application References: 1� Yasuda, M�, et al. 1995� Int. Immunol. 7: 251� (IP, Block)

Clone MRα4-1 LEAF™200106500 μg

$300

FITC20010350 μg$85

Rat CD49e (Integrin α5)Other Names: VLA-5 α chain, Integrin α5 chain, hmalpha5-1, hma5-1

CD49e is a 135 kD protein also known as α5 integrin or VLA-5 α chain� It is a member of the integrin family, expressed on thymo-cytes, splenic B cells, activated T cells, and mast cells� CD49e as-sociates with CD29 (integrin β1 chain) to form the fibronectin re-ceptor (VLA-5)� CD49e plays a critical role in both adhesion and T cell costimulation� The primary ligand for CD49e/CD29 (VLA-5) is fibronectin�

Clone: HMα5-1 Isotype: Armenian Hamster IgG

Immunogen: Affinity-purified mouse VLA-5 protein

Reactivity: Mouse, Rat

Rat CD45RC continued

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C57BL/6 mouse bone marrow cells stained with HMα5-1 PE

Applications: FC—Quality tested Activ, IP, Block—Reported in the literature

Application References: 1� Yasuda, M�, et al. 1995� Int. Immunol. 7:251� (IP, Block) ✦ 2� Noto, K�, et al. 1995� Int. Immunol. 7:835� (Block) ✦ 3� Lin, J�S�, et al. 2010� J. Leukoc. Biol. 88:95�

Clone HMα5-1 Purified103902500 μg

$215

LEAF™103908500 μg

$260

Biotin103904500 μg

$230

PE10390550 μg$105

103906200 μg

$265

Rat CD53Other Names: MOX44, OX-44, Tspan-25

CD53 is a 35–45 kD type III tetraspan membrane protein� It is ex-pressed on all leukocytes and a subset of thymocytes but not on platelets, erythrocytes, or non-hematopoietic cells� CD53 has been proposed to be involved in signal transduction and thymo-poiesis� It has been shown to interact with a number of proteins, including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4� Cross-linking of CD53 promotes B cell activation� The CD53 antigen is highly gly-cosylated and treatment with endoglycosidase F reduces the ap-parent molecular weight of this protein by approximately 25 kD�

Clone: OX-44 Isotype: Mouse IgG1, κImmunogen: Rat T cell blasts

LOU rat splenocytes stained with OX-44 APC

Applications: FC—Quality tested IP, IF, IHC (Paraffin), Cell Adhesion—Reported in the literature

Application References: 1� Lazo, P�A�, 1997� Cellular Immunol� 178: 132�

Clone OX-44 Purified204302500 μg

$260

Biotin204303100 μg

$130

PE204306100 μg

$195

APC204310100 μg$250

Rat CD54 (ICAM-1)

CD54 is an 85 kD protein also known as ICAM-1� It is a member of the immunoglobulin superfamily, expressed on high endothe-lial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, and thymic stromal cells� CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells)� ICAM-1 is an important protein in cell adhesion, inflammation, and immune

responses� CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan�

Clone: 1A29 Isotype: Mouse IgG1, κImmunogen: HEV-derived cell line Ax

Lou rat splenocytes stained with biotinylated 1A29,

followed by Sav-PE

Applications: FC—Quality tested IHC (Frozen/Paraffin), IP—Re-ported in the literature

Application References: 1� Clark, W�M�, et al. 1995� Mol. Chem. Neuropathol. 26:213� (IHC) ✦ 2� Tamatani, T�, et al. 1990� Int. Immunol� 2:165� (IP) ✦ 3� Tamatani, T�, et al. 1991� Eur. J. Immunol. 21:627� ✦ 4� Willenborg, D�O�, et al. 1996� J. Immunol. 157:1973� ✦ 5� McKechnie, N�M�, et al. 2002� IVOS 43:411� (IHC)

Clone 1A29 Purified202401100 μg

$105

Biotin202403100 μg

$130

PE202405100 μg

$140

Rat CD61 (Integrin β3)Other Names: Integrin β3 chain, gpIIIa

C57BL/6 mouse bone marrow cells stained with 2C9�G2 PE

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa� It associates with the inte-grin αv chain (CD51) to form the vitronectin receptor� In addition, CD61 can associate with the in-tegrin αIIb chain (CD41) to form the gpIIb/IIIa complex� CD61 is expressed on platelets, mega-karyocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells� CD61, in conjunc-

tion with CD41 or CD51, mediates adhesion to fibronectin, fibrin-ogen, vitronectin, thrombospondin, and von Willebrand factor� Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1)�

Clone: 2C9.G2 (HMβ3-1) Isotype: Armenian Hamster IgG

The 2C9�G2 (HMβ3-1) antibody reacts with mouse and rat CD61�

Immunogen: Vitronectin receptor protein from the mouse T cell hybridoma 2B4

Reactivity: Mouse, Rat

Applications: FC—Quality tested Block, Activ, IHC (Frozen)—Reported in the literature

Application References: 1� Kieffer, N�, et al. 1990� Annu. Rev. Cell Biol. 6:329� (Block) ✦ 2� Piali, L�, et al. 1995� J. Cell Biol. 130:451� (Block) ✦ 3� Ashkar, S�, et al. 2000� Science 287:860� (Block) ✦ 4� Schultz, J�F�, et al. 1995� J. Biol. Chem. 270:11522� (Block) ✦ 5� Moulder, K�, et al. 1991� J. Exp. Med. 173:343� (Activ) ✦ 6� Carlson T�R�, et al. 2008� 135:2193� ✦ 7� Yamaji, D�, et al. 2009� Genes Dev. 23:2382�

ContinuesLEAF™: Low Endotoxin, Azide-Free

Page 10: Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as LFA-2, is a member of the immunoglobulin ... $300 Alexa Fluor® 647 203408 100 μg $225

168 Toll Free 1.877.BIOLEGEND (246.5343)

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Clone 2C9.G2 (HMβ3-1) Purified

10430150 μg

$50

104302500 μg

$220

LEAF™10430950 μg$80

104310500 μg

$280

Biotin104304500 μg

$250

FITC10430550 μg$80

104306500 μg

$260

PE10430750 μg$100

104308200 μg

$260

Alexa Fluor® 48810431125 μg$85

Alexa Fluor® 64710431325 μg$85

104314100 μg

$195

Rat CD62L (L-Selectin)Other Names: LECAM-1, Ly-22, LAM-1, MEL-14

CD62L is a 74–95 kD glycoprotein also known as L-Selectin, LECAM-1, Ly-22, LAM-1, and MEL-14� It is a member of the Selec-tin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, subsets of thymocytes and NK cells� CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte “rolling” on activated endothelium� CD62L also contributes to neutrophil emigration at inflammatory sites� CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunc-tion with other markers) have been used to distinguish naïve, ef-fector, and memory T cells� CD62L has been reported to interact with O-glycans presented on CD34, GlyCAM-1, and MAdCAM-1�

Clone: OX-85 Isotype: Mouse IgG1, κImmunogen: Rat purified CD62L-CD4

LOU rat splenocytes stained with OX-85 PE and anti-rat CD3 (1F4) Alexa Fluor® 647

CD3 Alexa Fluor® 647

OX-

85 P

E

Applications: FC—Quality tested

Application References: 1� Voisine C�, 2002, J. Immunol� 169: 2284�

Clone OX-85 Purified202901100 μg

$105

FITC202905100 μg

$130

PE202912100 μg

$205

Alexa Fluor® 488202908100 μg$225

Alexa Fluor® 647202910100 μg$225

PerCP202914100 μg$250

Rat CD71 (Transferrin Receptor)Other Names: T9, TfR

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor� It is expressed on all proliferating cells, reticulocytes, and erythroid precursors� CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface�

Clone: OX-26 Isotype: Mouse IgG2a, κImmunogen: PHA-activated PVG rat lymph node cells

LOU rat bone marrow cells stained with OX-26

Alexa Fluor® 647

Applications: FC—Quality tested

Application References: 1� Jefferies, W�A�, et al. 1985� Immunology 54:333�

Clone OX-26 Purified204401100 μg

$105

FITC204405100 μg

$130 Alexa Fluor® 647

204408100 μg$225

Rat CD80 (B7-1)Other Names: B7, BB1, Ly-53

CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1� It is expressed on antigen present-ing cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4)� CD80/CD28 interactions are important for T and B cell costimulation, while CD80/CD152 leads to the inhibition of T cell responses�

Clone: 3H5 Isotype: Mouse IgG1, κImmunogen: HTLV-1–transformed Lewis-S1 rat T cell line

LOU rat splenocytes activated for 3 days with LPS, then stained

with CD80 (clone 3H5) PE�

Applications: FC—Quality tested IP, IHC (Frozen)—Reported in the literature

Application References: 1� Maeda, K�, et al. 1997� Int. Immunol. 9:993� (IP, Block) ✦ 2� Bluestone, J�A�, 1995� Immunity 2:555� (IP, Block) ✦ 3� Damoiseaux, J�G�, et al. 1998� J. Leuk. Biol. 64:803� (IP, Block, IHC) ✦ 4� Ho, CH�, et al. 2007� J. Virol. 81:4837� ✦ 5� Ho, C�, et al. 2009� Carcinogenesis 30:1443�

Clone 3H5 Purified200202500 μg

$220

PE20020550 μg$90

Rat CD81 (TAPA-1)

CD81 is a 26 kD nonglycosylated member of the TM4SF tet-raspanin superfamily and is also known as TAPA-1� CD81 is ex-pressed on T and B cells, NK cells, dendritic cells, thymocytes, en-dothelial cells, and fibroblasts� CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development� CD81 associates with several other cell-surface pro-teins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells; and CD4, CD8, and CD82 in T cells�

Clone: Eat-2 Isotype: Armenian Hamster IgG

The Eat-2 antibody reacts with mouse and rat CD81�

Rat CD61 continued

LEAF™: Low Endotoxin, Azide-Free

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Immunogen: CD81+ mouse B lymphoma 38C13

Reactivity: Mouse, Rat

Lou rat splenocytes stained with Eat-2 PE

Applications: FC—Quality tested IP, WB, Activ, Stim—Reported in the literature

Application References: 1� Maecker, H� T�, et al. 2000� Hybridoma 19:15� (IP, WB, Stim) ✦ 2� Clark, K� L�, et al. 2001� J. Immunol. 167:5115� (IP, WB, Activ) ✦ 3� Bhatnagar, S� and Schorey, J� S�, 2007� J. Biol. Chem. doi:10�1074/jbc�M702277200�

Clone Eat-2 Purified

10490150 μg

$70

104902500 μg

$215

LEAF™104908500 μg

$270

Biotin10490350 μg$80

PE10490550 μg$105

104906200 μg

$290

Rat CD86 (B7-2)Other Name: B70

CD86 is an 80 kD member of the immunoglobulin superfamily and is also known as B70 or B7-2� It is expressed on antigen pre-senting cells and activated T and B cells� CD86 is a ligand for CD28 and CD152 (CTLA-4)� It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions�

Clone: 24F Isotype: Mouse IgG1, κImmunogen: HTLV-1–transformed Lewis-S1 rat T cell line

Con A-stimulated (3 days) Lou rat splenocytes

stained with 24F PE

Applications: FC—Quality tested IF, IHC (Frozen)—Reported in the literature

Application References: 1� Maeda, K�, et al. 1997� Int. Immunol. 9:993� (IP, Block) ✦ 2� Bluestone, J�A� 1995� Immunity 2:555� (IP, Block) ✦ 3� Damoiseaux, J�G�, et al. 1998� J. Leuk. Biol. 64:803� (IP, Block, IHC)

Clone 24F Biotin20030350 μg$85

FITC20030550 μg$85

PE20030750 μg$90

200308200 μg

$200

Alexa Fluor® 647200314100 μg

$195

Rat CD90 (Thy-1)

CD90, also known as Thy-1, is a 28–30 kD GPI-linked membrane glycoprotein� It is a member of the immunoglobulin superfam-ily and has been shown to interact with CD45 in signal transduc-tion during lymphocyte proliferation and differentiation� CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells�

Clone: OX-7 Isotype: Mouse IgG1, κThe OX-7 antibody reacts with rat CD90 and mouse CD90�1 (Thy-1�1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90�2� This antibody has been reported to induce leukocyte activation, vascular permeability, induce apoptosis in glomerular mesangial cells, and induce glomerulonephritis in vivo�

Immunogen: Rat thymocyte Thy-1 antigen

Reactivity: Rat, Mouse

Lou rat thymocytes stained with OX-7 FITC

Applications: FC—Quality tested IHC (Frozen/Paraffin), IP, WB, IF—Reported in the literature

Application References: 1� Hermans, M�H�A�, et al. 1991� J. Histochem. Cytochem. 39:1627� (IHC) ✦ 2� Jeng, C�J�, et al. 1998� J. Cell Biol. 140:685� (IP, WB) ✦ 3� Nakashima, I�, et al. 1991� J.

Immunol. 147:1153� ✦ 4� Ishizu, A�, et al. 1995� Int. Immunol. 7:1939� ✦ 5� Eitner, F� 1997� Kidney. Int. 51:69� ✦ 6� Kawachi, H�, et al. 1992� Clin. Exp. Immunol. 88:399� ✦ 7� Hiramatsu, Y�, et al. 2010� J. Immunol. 87:703� (FC)

Clone OX-7 Purified

202501100 μg

$105

202502500 μg

$195

Biotin202510500 μg

$220

FITC202503100 μg

$130

202504500 μg

$295

PE20252350 μg$60

202524200 μg

$150

PE/Cy720251725 μg$135

202518100 μg

$285

APC202526100 μg

$240

APC/Cy720251925 μg$105

202520100 μg

$285

Alexa Fluor® 48820250525 μg$85

202506100 μg

$195

Alexa Fluor® 64720250725 μg$85

202508100 μg

$195

Alexa Fluor® 70020252725 μg$105

202528100 μg$225

Pacific Blue™20252125 μg$105

202522100 μg$225

PerCP202512100 μg$250

PerCP/Cy5.520251525 μg$90

202516100 μg

$275

Brilliant Violet 421™202529125 μl$175

Brilliant Violet 570™202531125 μl$205

Rat CD103 (αE2 Integrin)Other Names: OX-62, Integrin αE2

CD103, also known as Integrin αE2, is expressed on the major-ity of dendritic cells from rat lymphoid organs (such as spleen, lymph, lymph nodes, thymus) and nonlymphoid organs� OX-62 antigen is also expressed by dendritic epidermal γδ T cells and in-traepithelial T cells in intestine, but not by skin Langerhans cells and plasmacytoid dendritic cells� Among in vitro-generated DCs, it has been reported that a proportion of dendritic cells induced by GM-CSF and gelatin-coated flasks, or by flt-3 ligand (FL), GM-CSF, and IL-4 express OX-62� However, the dendritic cells obtained from culture with GM-CSF and IL-4 do not express OX-62 antigen�

Clone: OX-62 Isotype: Mouse IgG1

Immunogen: Density gradient enriched lymph veiled cells from PVG rats dendritic cells

ContinuesLEAF™: Low Endotoxin, Azide-Free

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LOU rat intestinal intraepithelial lymphocytes were stained with CD103 (clone OX-62) Alexa Fluor® 488 (left) or mouse IgG1, κ Alexa

Fluor® 488 isotype control (right) and CD3 Alexa Fluor® 647�

Mouse IgG1, κ Alexa Fluor® 488

CD

3 A

lexa

Flu

or®

647

OX-62 Alexa Fluor® 488

CD

3 A

lexa

Flu

or®

647

Applications: FC—Quality tested ICC, WB, IHC (Frozen)—Reported in the literature

Application References: 1� Brenan, M�, et al. 1992� J. Exp. Med. 175:1457� (IHC, WB) ✦ 2� Chen-Woan, M�, et al. 1995� J. Immunol. Methods 178:157� ✦ 3� Chen-Woan, M�, et al. 1996� J. Leuk. Biol. 59:196� ✦ 4� Brenan, M�, and Rees, D�J� 2000� Eur. J. Immunol. 30:1527� (IHC)

Clone OX-62 Purified205501100 μg

$105

Biotin205503100 μg

$130

FITC205505100 μg

$130

Alexa Fluor® 488205507100 μg

$185

Alexa Fluor® 647205509100 μg

$185

Rat CD106 (VCAM-1)Other Name: INCAM-110

CD106 is a 110 kD transmembrane protein also known as VCAM-1 and INCAM-110� It is an immunoglobulin superfamily member, ex-pressed on bone marrow stromal cells, myeloid cells, splenic den-dritic cells, and endothelial cells (activated by cytokines)� CD106 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin)� The interactions of CD106 and VLA-4 or LPAM-1 play an important role in binding of leukocytes to activated endothelial cells and leukocyte extravasation at inflammatory sites�

Clone: MR106 Isotype: Mouse IgG1, κImmunogen: Rat VCAM-1 transfected L5178Y cells

Lou rat bone marrow cells stained with MR106 PE

MR106 PE

Sid

e Sc

atte

r

Applications: FC—Quality tested IHC (Frozen), IP, WB—Reported in the literature

Application References: 1� Bevilacqua, M�P� 1993� Annu. Rev. Immunol. 11:767� (IHC) ✦ 2� Wingerd, K�L�, et al. 2002� J. Neuroscience 22:10772� (IHC) ✦ 3� Ogawa, T�, et al. 2004� Am J. Physiol. Gastrointest. Liver Physiol. 286:G702� (IHC)

Clone MR106 Purified

20040150 μg$65

200402500 μg

$220

PE20040350 μg$105

Rat CD126 (IL-6Rα)Other Names: IL-6 Receptor α chain, IL-6R

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R� It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells� High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 β chain (CD130 or gp130)� CD126 binds IL-6 with low affinity but does not signal� The β chain (gp130, CD130) does not bind IL-6 by itself but associates with the α-chain/IL-6 complex to initiate signal transduction� IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursors proliferation and/or differentiation�

Clone: D7715A7 Isotype: Rat IgG2b, κThe D7715A7 (15A7) antibody reacts with mouse and rat CD126�

Immunogen: OKT4 hybridoma cells

Reactivity: Mouse, Rat

Rat hybridoma cell line R12-31 stained with D7715A7 PE

Applications: FC—Quality tested Neut, IP, Block—Reported in the literature

Application References: 1� Coulie, P�G�, et al. 1990� Curr. Top. Microbiol. Immunol. 166:43� (Block, IP) ✦ 2� Vink, A�, et al. 1990� J. Exp. Med. 172:997� (Block, IP) ✦ 3� Sun, R�, et al. 2004� J. Immunol. 172:5648� (FC) ✦ 4� McKinney, W�J�, et al. 1998� Am. J. Respir. Cell Mol. Biol. 18:696� (Block)

✦ 5� Weigert, C�, et al. 2006� J. Biol. Chem. 281:7060� (Block, IP) ✦ 6� Koizumi, N�, et al. 2007� J. Immunol. 178:1767� (Block) ✦ 7� Walker, F�, et al. 2008� Blood 111:3978� (Neut) ✦ 8� Yadav, M�, et al. 2008� J. Immunol. 180:2772� (Block) ✦ 9� Sintes, J�, et al. 2010� J. Leukoc. Biol. 88:687

Clone D7715A7 Purified115802500 μg

$215

LEAF™11580750 μg$80

115808500 μg

$280

1158091 mg$510

Biotin11580350 μg

$75

115804500 μg

$250

PE11580525 μg$100

115806200 μg

$290

APC11581125 μg$105

115812100 μg$250

Rat CD134 (OX-40)Other Names: TNFRSF4, ACT35

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4)� This receptor is expressed on activated CD4+ and CD8+ T cells and B cells� The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28� Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases�

Clone: OX-40 Isotype: Mouse IgG2b, κImmunogen: Phytohemagglutinin (PHA) activated rat lymph node cells

Rat CD103 continued

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Con A-stimulated LOU rat splenocytes (Day 3) stained with anti-rat CD3 (1F4) Alexa

Fluor® 647 and OX-40 PE

OX-40 PE

CD

3 (1

F4) A

lexa

Flu

or®

647

Applications: FC—Quality tested IP, IHC (Frozen)—Reported in the literature

Application References: Al-Shamkhani� A�, et al. 1996� Eur J. Immunol. 26:1695�

Clone OX-40 Purified204501100 μg

$90

Biotin204503100 μg

$100

PE204508100 μg

$195

Alexa Fluor® 647204510100 μg$225

Rat CD147Other Names: OX-47, CE-9

CD147 is a 54 kD type I integral transmembrane protein also called extracellular metalloproteinase inducer (EMMPRIN) or M6 in humans, Basigin or gp42 in mouse, and OX-47 or CE-9 in rat� It is a heavily glycosylated Ig superfamily member and is expressed widely in hematopoietic and non-hematopoietic tissues� CD147 is an adhesion molecule and, through interaction with integrins, plays an important role in fetal, neuronal, blood cell, and extra-cellular matrix development�

Clone: OX-47 Isotype: Mouse IgG1, κImmunogen: T blasts from a mixed lymphocyte reaction

LOU rat splenocytes stained with OX-47 PE

Applications: FC—Quality tested IHC (Frozen), WB—Reported in the literature

Application References: 1� Glenn, M�, et al. 1995� Circulation 92:1919� (IHC) ✦ 2� Betsuyaku, T�, et al. 2003� Am. J. Respir. Cell Mol. Biol. 28:600� (WB)

Clone OX-47 Purified204601100 μg

$100

FITC204608100 μg

$175

PE204606100 μg

$195

APC204610100 μg$250

Rat CD152Other Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56� It is expressed on activated T and B lymphocytes� CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2)� Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4

negatively regulates cell-mediated immune responses� CD152 is thought to play a role in the induction and maintenance of im-munological tolerance as well as the development of protective immunity and thymocyte regulation�

Clone: WKH203 Isotype: Mouse IgG1, κ

LOU rat lymph node cells were stimulated with plate-bound CD3 and CD28 for 3 days, then surface stained with CD4 PerCP, followed by fixation and permeabilization with BioLegend’s FOXP3 Fix/Perm

Buffer Set and intracellularly stained with FOXP3 Alexa Fluor® 647, and CD152 (Clone WKH203) PE (left) or mouse IgG1, κ PE isotype control

(right)� The data were analyzed by gating on CD4-positive cells�

FOXP3 Alexa Fluor® 647

CD

152

(clo

ne

WKH

203)

PE

FOXP3 Alexa Fluor® 647

Mou

se Ig

G1,

κ P

E is

otyp

e co

ntro

l

Applications: FC—Quality tested IP—Reported in the literature

Application References: 1� Lin, C-H� and Hunig, T�, et al. 2003� Eur. J. Immunol. 33:626� (IP) ✦ 2� Nessi, V�, et al. 2010� J. Immunol. 185:5656�

Clone WKH203 Purified203002100 μg

$105

PE20300725 μg

$95

Rat CD161Other Names: NKR-P1, CD161a/CD161b, NKR-P1A/NKR-P1B

CD161 molecules, also known as NKR-P1, are a family of about 30 kD type II transmembrane C-type lectin-like receptors and are expressed on the cell membrane as disulphide-linked homodi-mers� Rat NKR-P1 receptors are primarily expressed on NK cells, a subset of T cells, dendritic cells, and activated monocytes� Car-bohydrate antigens with Gal-Nac and GlcNac moieties are the ligands for NKR-P1 molecules� CD161 receptors are thought to be involved in the regulation of NK and NKT cell function� Three rat NKR-P1 genes have been described: NKR-P1A, NKR-P1B, NKR-P1B* (or NKR-P1D)� 10/78, similar to 3�2�3 antibody, recognizes a com-mon epitope of NKR-P1A (CD161a) and NKR-P1B (CD161b)� NKR-P1A does not contain ITIM structure and is an activating recep-tor, while NKR-P1B contains an ITIM and displays an inhibitory function�

Clone: 10/78 Isotype: Mouse IgG1, κ

Immunogen: LEW rat splenic NK cells

Rat splenocytes stained with 10/78 Alexa Fluor® 647

ContinuesLEAF™: Low Endotoxin, Azide-Free

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Applications: FC—Quality tested IF, IP, WB, IHC (Frozen)—Reported in the literature

Application References: 1� Sedgwick, J�D�, et al. 1998� J. Immunol. 160: 5320� ✦ 2� Tiba, O�, et al. 2002� Vet. Res. 33(3): 327� (IHC)

Clone 10/78 Purified203101100 μg

$105

FITC203105100 μg

$130

Alexa Fluor® 647203110100 μg

$195

Rat CD172a (SIRPα)Other Names: src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, P84

Rat CD172a, also known as signal-regulatory protein α (SIRPα), is a 75–110 kD transmembrane glycoprotein involved in the recep-tor tyrosine kinase coupled signaling pathway� It belongs to the Ig superfamily primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons� CD172a serves as a sub-strate of activated receptor tyrosine kinases (RTKs)� The interac-tion of the CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhe-sion and transmigration, T cell activation, macrophage fusion and phagocytosis� CD47 (IAP) is an extracellular ligand for CD172a�

Clone: OX-41 Isotype: Mouse IgG2a, κImmunogen: Rat peritoneal macrophages

LOU rat bone marrow cells stained with OX-41 PE (gated on granulocyte population)

Applications: FC—Quality tested IHC, IP, WB—Reported in the literature

Application References: 1� Robinson, A�P�, et al. 1986� Immunology 57:239� (IP, IHC) ✦ 2� Thai, QA�, et al. 1999� Stroke 30:140� (IHC) ✦ 3� Adams, S�, et al. 1998� J. Immunol. 161:1853� (WB)

Clone OX-41 Purified204702500 μg

$260

PE204706100 μg

$195

Rat CD178 (FasL, CD95L)Other Names: Fas Ligand, Apo-1 Ligand, CD95 Ligand

CD178 is a 40 kD member of the TNF/NGF superfamily and is also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand� Cell surface CD178 is expressed on activated T cells and in the testis and eye� CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and para-crine T cell death� CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites� CD178 can be cleaved from the surface by metalloproteases, and “soluble” CD178 may block the activities of membrane-bound CD178� CD178

binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance� CD178/CD95 interac-tions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival�

Clone: MFL4 Isotype: Armenian Hamster IgG

The MFL4 antibody is cross-reactive with rat and mouse FasL�

Immunogen: B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells

Reactivity: Mouse, Rat

Applications: FC—Quality tested Block—Reported in the literature

Application References: 1� Trinité, B�, et al. 2000� J. Immunol. 165:4202� (Block) ✦ 2� Kayagaki, N�, et al. 1997� P. Natl. Acad. Sci. USA 94:3914� (Block) ✦ 3� Chang, C�L�, et al. 2007� J. Immunol. 178:6984� (Block)

Clone MFL4 Purified106702500 μg

$215

LEAF™106706500 μg

$300

Biotin10670350 μg

$75

Rat CD200Other Names: OX-2, MRC

LOU rat splenocytes stained with OX-2 PE and RT1D (OX-17) FITC (left panel) and double stained with mouse IgG1 (MOPC-

21) PE isotype control and RT1D (OX-17) FITC (right panel)�

RT1D (OX-17) FITC

OX-

2 PE

RT1D (OX-17) FITC Mou

se Ig

G1

(MO

PC-2

1) P

E is

otyp

e co

ntro

l

CD200 is a 41–47 kD type I transmembrane glycoprotein also known as OX-2 or MRC� It is a member of the immunoglobulin superfamily primarily expressed on B cells, activated T cells, thy-mocytes, neurons, some endothelium, and dendritic cells� The in-teraction of CD200 with its receptor, CD200R (OX-2R), plays roles in downregulation of myeloid cell activity and inhibition of dendritic cell-induced Th1 cytokine production as well as in modulating microglial activation�

Clone: OX-2 Isotype: Mouse IgG1, κImmunogen: Rat thymocyte membrane glycoproteins

Applications: FC—Quality tested

Application References: 1� Dick, A�D�, et al. 2001� Invest Ophthalmol Vis Sci 42: 170�

Clone OX-2 Purified204801100 μg

$95

FITC204805100 μg

$130

PE204807100 μg

$175

Rat CD161 continued

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Rat CD200 ReceptorOther Names: OX-2R, CD200RCD200 receptor is a 60–100 kD type I transmembrane glycopro-tein also known as OX-2R or CD200R� It is a member of the im-munoglobulin superfamily that is primarily expressed on mac-rophages/monocytes, granulocytes, mast cells, and subsets of T (memory T) and B (memory B and palsmablasts) cells� The in-teraction of CD200R with its ligand, CD200, plays roles in down-regulation of myeloid cell activity and inhibition of dendritic cell- induced Th1 cytokine production as well as in modulating microglial activation�

Clone: OX-102 Isotype: Mouse IgG1, κImmunogen: Membrane fraction of thioglycollate-elicited rat peripheral cells

LOU rat peritoneal cells stained with OX-102 FITC

Applications: FC—Quality tested WB, IP, IHC (Frozen)—Reported in the literature

Application References: 1� Wright, G�J�, et al. 2000� Immunity 13: 233� ✦ 2� Dick, A�D�, et al. 2001� Invest Ophthalmol Vis Sci 42: 170�

Clone OX-102 Purified204901100 μg

$90

FITC204905100 μg

$130

Rat CD252 (OX40 Ligand)Other Names: OX-40L, CD134L, TNFSF4OX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4) and is also known as CD252 or CD134L� It is expressed on dendritic cells, peritoneal macrophages, and activated B cells� The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation�

Clone: ATM-2 Isotype: Mouse IgG1, κ

PMA-stimulated (2 days) Lou rat splenocytes stained

with biotinylated ATM-2, followed by Sav-PE

Immunogen: ACI-S1 rat T-cell lymphoma cell line

Applications: FC—Quality tested

Application References: 1� Satake, Y�, et al. 2000� Biochem. Biophys. Res. Commun. 270: 1041�

Clone ATM-2 Purified200502500 μg

$240

PE20050550 μg$115

Rat CD278 (ICOS)Other Names: Inducible COStimulatory molecule, H4CD278 is a 47–57 kD protein also known as inducible costimula-tory molecule (ICOS) and H4� This protein is homologous to the CD28/CTLA-4 proteins� ICOS is expressed on activated T cells and

a subset of thymocytes, and can costimulate T cells and induce proliferation� In addition, ICOS is involved in humoral immune re-sponses (B cell germinal center formation)� The ICOS ligand is B7h/B7RP-1 or B7-H2� ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development�

Clone: C398.4A Isotype: Armenian Hamster IgG

The C398�4A antibody reacts with human, mouse and rat ICOS�

Immunogen: Mouse T cell clone D10�G4�1

Reactivity: Human, Mouse, Rat, Cross-Reactivity: Pig

PHA-activated (3 days) human peripheral blood lymphocytes

stained with CD278 (clone C398�4A) Pacific Blue™�

Applications: FC—Quality tested IF, IP, IHC (Frozen), Costim—Re-ported in the literature

Application References: 1� Redoglia, V�, et al. 1996� Eur. J. Immunol. 26:2781� (FC, IP, Costim) ✦ 2� Yagi, J�, et al. 2003� J. Immunol. 171:783� (FC) ✦ 3� Arimura, Y�, et al. 2002� Int. Immunol. 14:555� (Costim) ✦ 4� Arimura, Y�, et al. 2004� J. Biol. Chem. 279:11408� (Costim)

Clone C398.4A Purified313502500 μg

$230

LEAF™31351150 μg$90

313512500 μg

$280

Biotin31350350 μg$80

313504500 μg

$280

FITC31350525 μg$65

313506100 μg

$140

PE31350725 μg$80

313508100 μg

$160

PE/Cy731351925 μg$90

313520100 μg

$255

APC31350925 μg$100

313510100 μg$200

Alexa Fluor® 488313514100 μg

$195

Alexa Fluor® 64731351525 μg$85

313516100 μg

$195

Pacific Blue™31352125 μg$90

313522100 μg

$255

PerCP/Cy5.531351725 μg$100

313518100 μg

$275

Rat αE2 Integrin (CD103) see Page 169

Rat Endothelium Antigen

The OX-43 antibody reacts with a 90 kD protein which is ex-pressed on vascular endothelial cells in all rat tissues (except in brain capillaries and invasive capillary sprouts), peritoneal macro-phage and a subset of alveolar macrophages� This antibody also reacts with circulating erythrocytes�

Clone: OX-43 Isotype: Mouse IgG1, κImmunogen: Rat peritoneal macrophages

LOU rat peritoneal macrophages stained

with OX-43 FITC

Applications: FC—Quality tested IHC, IP—Reported in the literature

Clone OX-43 Purified205001100 μg

$105

FITC205003100 μg

$130

LEAF™: Low Endotoxin, Azide-Free

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174 Toll Free 1.877.BIOLEGEND (246.5343)

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Rat Fas Ligand (CD95L, CD178) see page 172

Rat FOXP3Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

FOXP3 is a 50–55 kD transcription factor also known as Forkhead box protein P3, Scurfin, JM2, or IPEX� It is proposed that it is a master regulatory gene and more specific marker of T regula-tory cells than most cell surface markers (such as CD4 and CD25)� Transduced expression of FOXP3 in CD4+/CD25– cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regula-tory cell phenotype� FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in “scurfy” mice� Overexpression of FOXP3 has been shown to lead to a hypoactive immune state, suggesting that this transcriptional factor is a central regulator of T cell activity�

Clone: 150D Isotype: Mouse IgG1, κThe 150D monoclonal antibody reacts with human, mouse, and rat FOXP3�

Immunogen: Full-length FOXP3 protein

Reactivity: Human, Mouse, Rat Cross-Reactivity: Cynomolgus, Rhesus, Baboon

Lou rat splenocytes surface stained with CD4 PE, then intracellularly stained with

150D Alexa Fluor® 488

150D Alexa Fluor® 488

CD

4 PE

Applications: ICFC—Quality tested WB, IHC (Frozen/Paraffin)—Re-ported in the literature

Application References: 1� Roncador, G�, et al. 2005 Eur. J. Immunol. 35: 1681� ✦ 2� Luke, P�P�W�, et al. 2006� Amer. J. Transplant. 6(9):2023� ✦ 3� Bamias, G�, et al. 2007� J. Immunol. 178:1809� ✦ 4� Valencia, X�, et al. 2007� J. Immunol. 178:2579� ✦ 5� Davidson, T�S�, et al. 2007� J. Immunol. 178:4022� ✦ 6� MacDonald, K�, et al. 2007� Blood

doi:10�1182/blood-2007-01-067249� ✦ 7� Müller, M�, et al. 2007� J. Immunol. 179:2774� ✦ 8� Jaffar, Z�, et al. 2007� J. Immunol. 179:6193�

Clone 150D Purified

32000125 μg$110

320002100 μg$225

PE32000725 tests

$165

320008100 tests

$325

Alexa Fluor® 48832001125 tests

$160

320012100 tests

$315

Alexa Fluor® 64732001325 tests

$160

320014100 tests

$315

FOXP3 Flow KitOther Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

BioLegend’s FOXP3 Flow Kit is designed and formulated specifi-cally for immunofluorescence staining and flow cytometric analy-sis of mouse/human/rat FOXP3+ Treg cells in a mixed lymphocyte population� This kit is composed of fluorochrome-conjugated anti-mouse/human/rat FOXP3 antibody, matched isotype con-trol, and the critical buffers� It is easy to use for identification of FOXP3+ Treg cells�

Clone: 150D

Reactivity: Rat, Human, Mouse, Cross-Reactivity: Cynomolgus, Rhesus, Baboon

Materials Provided: 1� Fluorochrome-conjugated anti-mouse/rat/human FOXP3

(Clone 150D) 2� Fluorochrome-conjugated Mouse IgG1, κ isotype control

(Clone MOPC-21) 3� FOXP3 Fix/Perm Buffer Set

CD4+CD25+ (top) or CD4+CD25– (bottom)

C57BL/6 mouse splenocytes intracellular stained with

150D Alexa Fluor® 488

Applications: ICFC—Quality tested

Application References: 1� Roncador, G�, et al. 2005� Eur. J. Immunol. 35: 1681� ✦ 2� Luke, P�P�W�, et al. 2006� Amer. J. Transplant. 6(9): 2023� ✦ 3� Bamias, G�, et al. 2007� J. Immunol. 178: 1809� ✦ 4� Valencia, X�, et al. 2007� J. Immunol. 178: 2579� ✦ 5� Davidson, T�S�, et al. 2007� J. Immunol. 178: 4022� ✦ 6� MacDonald, K� PA�, et al. 2007� Blood doi: 10�1182/blood-2007-01-067249� ✦ 7� Müller, M�, et al. 2007� J. Immunol. 179: 2774� ✦ 8� Jaffar, Z�, et al. 2007� J. Immunol. 179: 6193�

Clone 150D PE Kit

320019 25 tests $325

Alexa Fluor® 488 Kit

320017 25 tests $295

Alexa Fluor® 647 Kit

320021 25 tests $295

Rat ICAM-1 (CD54) see page 167

Rat ICOS (CD278) see page 173

Rat IgMOther Name: Immunoglobulin M

The MRM-47 monoclonal anti-body reacts with rat immuno-globulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley)� It does not react with other iso-types� The MRM-47 antibody can be used as a primary or second-ary reagent for ELISA or immuno-fluorescent analysis�

Clone: MRM-47 Isotype: Mouse IgG

Immunogen: Mixed rat Igs

Applications: FC—Quality tested ELISA—Reported in the literature

LOU rat splenocytes were stained with MRM-47 FITC

and MHC class II antigen RT1D (14-4-4S) Alexa Fluor® 647�

MRM-47 FITC

RT1D

Ale

xa F

luor

® 64

7

LEAF™: Low Endotoxin, Azide-Free

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Clone MRM-47 Purified408901100 μg

$145

Biotin408903100 μg

$155

FITC408905100 μg

$165

Rat IL-2Rα (CD25) see page 163

Rat IL-6Rα (CD126) see page 170

Rat Integrin α4 (CD49d) see page 166

Rat Integrin α5 (CD49e) see page 166

Rat Integrin αM/αX (CD11b/c) see page 163

Rat Integrin β1 (CD29) see page 164

Rat Integrin β3 (CD61) see page 167

Rat L-Selectin (CD62L) see page 168

Rat Myeloid Lineage

The OX-82 antibody reacts with a 35 kD protein which is ex-pressed on rat myeloid cells� Rat hematopoietic stem cells are CD71-CD90+OX-82+�

Clone: OX-82 Isotype: Mouse IgG1, κImmunogen: Anemic rat bone marrow

LOU rat bone marrow cells stained with OX-82 FITC (gated

on myeloid cell population)

Applications: FC—Quality tested WB—Reported in the literature

Application References: 1� Crook, K�, et al. 1996� Dev. Immunol. 4: 235�

Clone OX-82 Purified205101100 μg

$95

FITC205103100 μg

$130

Rat OX-40 Ligand (CD252) see page 173

Rat PCNAOther Names: Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein

PCNA, also known as DNA polymerase δ auxiliary protein, is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp ex-pressed in the nucleus of all proliferating cells� A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand� PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody�

Clone: PC10 Isotype: Mouse IgG2a, κReactivity: Human, Mouse, Rat, Cross-Reactivity: Other species

Immunogen: Recombinant rat PCNA

MOLT-4 cells were fixed in 70% ethanol then

stained with PC10 PE�

Applications: ICFC—Quality tested IHC, IP, WB, IF—Reported in the literature

Application References: 1� Ogata, K�, et al. 1985� J. Immunol. 135:2623� ✦ 2� Garcia, R�, et al. 1989� Am. J. Pathol. 134:733� (IHC) ✦ 3� Landberg, G�, et al. 1990� Exp. Cell. Res. 187:111� (ICFC) ✦ 4� Yu, C�, et al. 1991� Histopathology 19:29� (IHC) ✦ 5� Wilkins, B�, et al. 1992� J. Pathol.

166:45� (IHC) ✦ 6� Chou, H-Y� E�, et al. 2006� J. Biol. Chem. 10:1074� (IF) ✦ 7� Fulvio, M� D�, et al. 2006� Oncogene 25:3932� (WB)

Clone PC10 Purified

30790125 μg

$50

307902100 μg

$95

Biotin30790325 μg$80

307904100 μg

$190

PE307908

100 tests$250

Alexa Fluor® 48830790925 tests

$105

Alexa Fluor® 647307912

100 tests$225

Rat RT1AOther Names: MHC Class I, Major Histocompatibility Complex I, MHC I, RT1�A

The rat monomorphic MHC class I antigen RT1A is expressed on nucleated cells (except neurons and glial cells) and erythrocytes� RT1A is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins�

Clone: OX-18 Isotype: Mouse IgG1, κImmunogen: RT1B-depleted membrane glycoproteins iso-lated from Wistar rat spleen

LOU rat splenocytes stained with OX-18 PE

Applications: FC—Quality tested IHC (Frozen), IP—Reported in the literature

Clone OX-18 Purified205201100 μg

$105

PE205208100 μg

$195

Rat RT1BOther Names: MHC Class II, Major Histocompatibility Complex II, MHC II, RT1�B

The rat monomorphic MHC class II antigen RT1B is equivalent to mouse I-A antigen and is expressed on antigen presenting cells including B cells, dendritic cells, macrophages, Langerhans cells, thymic epithelial cells, and LPS-stimulated microglial cells� RT1B is involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins�

Continues

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Clone: OX-6 Isotype: Mouse IgG1, κThe OX-6 antibody cross-reacts with mouse I-Ak and I-As alloanti-gens and a major subset of splenocytes from NOD (I–A[g7]) mice�

Immunogen: Ia-like glycoproteins from Wistar thymocytes

LOU rat splenocytes stained with OX-6 FITC and CD3 APC

CD3 APC

OX-

6 FI

TC

Applications: FC—Quality tested

Application References: 1� Fox C�C�, et al. 1994� Cell Immunol. 158:253� ✦ 2� Chen-Woan M�, et al. 1996� J. Leukocyte Biol. 59:196� ✦ 3� Damoiseaux J�G�, et al. 1998� J. Leukocyte Biol. 64:803�

Clone OX-6 Purified205301100 μg

$105

Biotin205303100 μg

$130

FITC205305100 μg

$130

PE205308100 μg

$195

Rat RT1DOther Name: MHC Class II

The α chain rat monomorphic MHC class II antigen RT1D is equiva-lent to mouse I-E antigen and is expressed on antigen presenting cells including B cells, dendritic cells, macrophages, Langerhans cells, and thymic epithelial cells� RT1D is involved in antigen pre-sentation to T cells expressing CD3/TCR and CD4 proteins�

Lou rat splenocytes stained with biotinylated 14-4-4S, followed by Sav-PE

Clone: 14-4-4S Isotype: Mouse (C3H�SW) IgG2a, κThe 14-4-4S antibody reacts with rat MHC class II antigen RT1D and mouse I-Ek alloantigen, and cross-reacts with mouse with b, f, q, and s haplotypes�

Immunogen: C3H mouse skin graft and splenocytes

Reactivity: Rat, Mouse I-Ek, Cross-reacts with mice of H-2d,p,r haplotypes� Mice of the

H-2b,f,q,s haplotypes do not express I-E antigens�

Applications: FC—Quality tested IF, IP, IHC (Frozen)—Reported in the literatureApplication References: 1� Ozato, K�, et al. 1980� J. Immunol. 124:533� ✦ 2� Nei, U�, et al. 1994� Int. Immunol. 6:61� (IP) ✦ 3� Harton, J�A�, et al. 1991� Immunogenetics 34:358� ✦ 4� Roy, M�, et al. 1995� Eur. J. Immunol. 25:596� ✦ 5� Farr, A�, et al. 1996� Eur. J. Immunol. 26:3185� (IHC) ✦ 6� Desmet, C�, et al. 2004� J. Immunol. 173:5766� (IHC) ✦ 7� Shih, F� F�, et al. 2006� J. Immunol. 176:3438� (FC)

Clone 14-4-4S Purified

110202500 μg

$215

Biotin110204500 μg

$230

FITC11020550 μg

$70

Alexa Fluor® 488110210100 μg

$195

Alexa Fluor® 64711021125 μg$85

Clone: OX-17 Isotype: Mouse IgG1, κImmunogen: Wistar rat splenocyte membrane extract

LOU rat splenocytes stained with OX-17 FITC

Applications: FC—Quality tested IHC (Frozen)—Reported in the literature

Application References: 1� Holmberg, et al. 2006� J. Immunol� 176 : 1172�

Clone OX-17 Purified205401100 μg

$105

FITC205405100 μg

$130

PE205408100 μg

$195

Rat Thy-1.1 (CD90.1) see page 169

Rat VCAM-1 (CD106) see page 170

Rat TCR α/βOther Names: α/β T Cell Receptor, α/β TCR

TCR α and β are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most periph-eral T cells, intestinal epithelial lymphocytes, and thymocytes� The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation� The α/β TCR recognizes pep-tide bound to MHC class I and II�

LOU rat splenocytes stained with R73 FITC

Clone: R73 Isotype: Mouse IgG1, κImmunogen: Rat T cell blasts & erythrocytes

Reactivity: Rat, Cross-Reactivity: Cynomolgus, Rhesus

Applications: FC—Quality tested IHC (Frozen), WB, IF—Reported in the literature

Application References: 1� Hunig, T�, et al. 1989� J. Exp. Med. 169:73� (IHC) ✦ 2� Mitnacht, R�, et al. 1995� Eur. J. Immunol. 25:328� (IHC) ✦ 3� Brenan, M�, et al. 1997� Eur. J. Immunol. 27:3070� (WB) ✦ 4� Yoshino, N�, et al. 2000� Exp. Anim. 49:97�

Clone R73 Purified

201102500 μg

$180

FITC20110550 μg$80

PE20110750 μg$85

APC201110100 μg$260

Alexa Fluor® 488201114100 μg

$195

Alexa Fluor® 647201116100 μg

$195

Rat RT1B continued

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Rat TCR γ/δOther Names: γ/δ T Cell Receptor, γ/δ TCR

γ/δ TCR expression is observed on a subset of cells in the thymus, intestinal epithelium, skin, liver, peripheral lymphoid tissues, and peritoneum� The γ/δ TCR is involved in the antigen recognition of some bacterial and tumor-associated antigens presented by MHC class I�

Clone: V65 Isotype: Mouse IgG1, κ

LOU rat splenocytes stained with CD3 (1F4) APC and V65 PE

V65 PE

CD

3 (1

F4) A

PC

Applications: FC—Quality tested IP, IHC—Reported in the literature

Application References: 1� Kuhnlein, P�, et al. 1994� J. Immunol. 153: 979� ✦ 2� Pelegri, C�, et al. 1996� Arthritis Rheum. 39(2): 204� ✦ 3� Tsuchiya, T�, et al. 2002� Amer. J. of Respir. and Crit. Care Med. 165: 1640�

Clone V65 Purified202601100 μg

$105

FITC202603100 μg

$130

PE20260550 μg$85

Rat TCR Vβ8.2/8.4Other Name: Vβ 8�2/8�4 T Cell Receptor

The TCR β chain interacts with the TCR α chain and CD3 to form an antigen binding and signaling complex� TCR α and β are members of the immunoglobulin superfamily expressed on most peripheral T cells, intestinal epithelial lymphocytes, and thymocytes� TCR Vβ 8�2/8�4 is expressed on a subset of TCR α/β-bearing T cells� The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation� The α/β TCR recognizes pep-tide bound to MHC class I and II�

Clone: R78 Isotype: Mouse IgG1, κThe R78 antibody recognizes the specific T cell receptor β chain Vβ8�2/8�4�

Immunogen: Rat Vβ8�2 TCR-expressing rat/mouse T cell hybrid 56�4

Applications: FC—Quality tested IHC (Frozen), IF—Reported in the literature

Application References: 1� Torres-Nagel, N�, et al. 1992� Immunogenetics 37: 305� ✦ 2� Imrich, H�, et al. 1995� Eur. J. Immunol. 25: 1960�

Clone R78 Purified20120150 μg$60

Alexa Fluor® 488201206100 μg

$195

Dog CD34Other Name: Mucosialin

CD34 is thought to function as an adhesion molecule for early he-matopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal mucosialin� It is selectively ex-pressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, and embry-onic fibroblasts� CD34+ hematopoietic progenitors may be mo-bilized to the peripheral blood by treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell fac-tor� Furthermore, CD34 is expressed on some canine leukemias�

Clone: 2E9 Isotype: Mouse IgG1, κReactivity: Dog

CLL-1390 cell line stained with 2E9 PE

Applications: FC—Quality tested

Clone 2E9 Purified250102100 μg

$175

Biotin25010325 μg$105

PE25010525 μg$125

Page 20: Rat Immunology - · PDF fileRat Immunology Rat CD2 Other Names: LFA-2, T11 CD2, also known as LFA-2, is a member of the immunoglobulin ... $300 Alexa Fluor® 647 203408 100 μg $225