Product Instructions E96PNT EN Peanut Protein ELISA Kit FR ... 3 EN (English) To reduce the risks...

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Transcript of Product Instructions E96PNT EN Peanut Protein ELISA Kit FR ... 3 EN (English) To reduce the risks...

  • Product Instructions E96PNT3

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    땅콩 단백질 ELISA 키트 ELISA Kit Protein Kacang Tanah طقم اليزا بروتين الفول السوداني

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  • 1

    (English) EN Issue Date: 2017-11

    Product Instructions

    Peanut Protein ELISA Kit Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative analysis of peanut proteins.

    Product Description and Intended Use The 3M™ Peanut Protein ELISA Kit is intended for the detection of peanut proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients, and processed food products.

    The 3M Peanut Protein ELISA Kit utilizes a sandwich ELISA. The peanut proteins present in the sample react with the anti-peanut antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-peanut antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound peanut protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of peanut protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of peanut protein in the test sample. The quantity of peanut protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

    The 3M Peanut Protein ELISA Kit is intended for use in a laboratory environment by professionals trained in laboratory techniques. 3M has not documented the use of this product in industries other than food or beverage. For example, 3M has not documented this product for testing pharmaceutical, cosmetic, clinical or veterinary samples. The 3M Peanut Protein ELISA Kit has not been evaluated with all possible food products, food processes and testing protocols.

    The 3M Peanut Protein ELISA Kit contains 96 wells, described in Table 1.

    Table 1. Kit components

    Item Identification Preparation (see Reagent Preparation section for details)

    Storage Stability

    3M™ Peanut Protein ELISA Wells

    One foil bag with a plate of 96 removable antibody coated wells.

    Ready to use. 2-8°C in sealed foil bag with desiccant.

    Re-seal foil bag containing unused wells and desiccant. Store at 2-8°C to maintain stability until the expiration date of the kit.

    3M™ Peanut HRP Conjugate (10X)

    One vial with 1.5 mL of 10X Horseradish Peroxidase (HRP) Conjugated antibody (10X).

    Dilute 1/10 immediately prior to use to make a 1X working solution.

    2-8°C in the dark.

    The 10X conjugate is stable until the expiration date of the kit.

    3M™ Peanut Protein Standard Concentrate

    One vial with a known concentration of peanut protein.

    Refer to the ELISA Procedure Section for standard preparation.

    2-8°C. Do not freeze.

    3M Peanut Protein Standard Concentrate is stable until the expiration date of the kit.

    3M™ Diluent (5X) One bottle with 50 mL of 5X Diluent.

    Dilute 1/5 immediately prior to use to make a 1X working solution.

    2-8°C The 5X 3M Diluent Solution is stable until the expiration date of the kit.

    3

  • 2

    (English) EN

    3M™ Wash Solution (20X)

    One bottle with 50 mL of 20X wash solution.

    Dilute 1/20 to make a 1X working solution.

    2-8°C for both 1X working solution and 20X Wash Solution concentrate.

    The 20X 3M Wash Solution is stable until the expiration date of the kit. The 1X Wash Solution is stable for at least one week after preparation.

    3M™ Extraction Buffer E26 (4X)

    One bottle with 120 mL of 4X extraction buffer.

    Dilute 1/4 to make a 1X working solution. The working solution should be heated to 50-60°C before use.

    2-8°C for both 1X working solution and 4X 3M Extraction Buffer concentrate.

    The 1X Extraction Buffer and 4X 3M Extraction Buffer are stable until the expiration date of the kit.

    3M™ Chromogenic Substrate Solution

    One bottle with 12 mL of 3,3’,5,5’-tetramethylbenzidine (TMB).

    Ready to use. 2-8°C in the dark.

    Protect from light. The 3M Chromogenic Substrate Solution is stable until the expiration date of the kit.

    3M™ Stop Solution One bottle of 12 mL of 0.3 M sulfuric acid.

    Ready to use. 2-8°C The 3M Stop Solution is stable until the expiration date of the kit.

    Materials not provided in the kit:

    • Precision pipettes and pipette tips to collect 10 to 100 μL • Test tubes • Microtiter plate washer/aspirator • Distilled or deionized water • Microtiter plate reader • Assorted labware for the preparation of reagents and buffer solutions • Timer • Vortex • Shaking water bath or shaking incubator • Orbital shaker

    Safety The user should read, understand, and follow all safety information in the instructions for the 3M Peanut Protein ELISA Kit. Retain the safety instructions for future reference.

    WARNING: Indicates a hazardous situation, which, if not avoided, could result in death or serious injury and/or property damage.

    NOTICE: Indicates a potentially hazardous situation, which, if not avoided, could result in property damage.

    W WARNING To reduce the risks associated with exposure to chemicals: • Dispose according to current local/regional/national/industry standards and regulations. • The user must train its personnel in current proper testing techniques; for example, Good Laboratory Practices1 or

    ISO/IEC 170252. • Always follow standard laboratory safety practices, including wearing appropriate protective apparel and eye

    protection while handling reagents. • Avoid skin contact with the 3M Stop Solution, see Safety Data Sheet for additional safety information.

  • 3

    (English) EN To reduce the risks associated with false-negative results leading to the release of contaminated product: • Store the 3M Peanut Protein ELISA Kit as indicated on the package and in the product instructions. • Use the 3M Peanut Protein ELISA Kit for food and environmental samples that have been validated internally or by

    a third party. • Follow the protocol and perform the tests exactly as stated in the product instructions. • 3M has not documented the use of the 3M Peanut Protein ELISA Kit in industries other than food or beverage. For

    example, 3M has not documented this product for testing pharmaceutical, cosmetic, clinical or veterinary samples.

    To reduce the risks associated with inaccurate results leading to the release of contaminated product: • Always use the 3M Peanut Protein ELISA Kit by the expiration date. • Always prepare working solutions using the 3M Peanut Protein ELISA Kit concentrated reagents at 20-25°C

    temperature. • Do not freeze the 3M Peanut Protein Standard Concentrate. • If Chromogenic Substrate Solution turns blue, do not use. Follow Good Laboratory Practices1 to avoid cross-

    contamination of 3M Chromogenic Substrate Solution.

    NOTICE To reduce the risks associated with inaccurate results: • Sample stability after extractions has not been evaluated. The ELISA procedure should be carried out, right after

    sample extraction. • Handle 3M Peanut Protein Standards following Good Laboratory Practices1 to prevent cross-contamination of

    samples.

    Consult the Safety Data Sheet for additional information.

    For information on documentation of product performance, visit our website at www.3M.com/foodsafety or contact your local 3M representative or distributor.

    User Responsibility Users are responsible for familiarizing themselves with product instructions and information. Visit our website at www.3M.com/foodsafety, or contact your local 3M representative or distributor for more information.

    As with all test methods used for food analysis the test matrix can influence the results. When selecting a test method, it is important to recognize that external factors such as sampling methods, testing protocols, sample preparation, handling, and laboratory technique may influence results. The food sample itself may influence results.

    It is the user’s responsibility in selecting any test method or product to evaluate a sufficient number of samples to satisfy the user that the chosen test method meets the user’s criteria.

    It is also the user’s responsi