Polyclonal ANTIBODY CUEDC2 - ptglab.com filePBS with 0.02% sodium azide and 50% glycerol pH 7.3....
2
CUEDC2 Polyclonal ANTIBODY Catalog Number:20123-1-AP For Research Use Only www.ptglab.com Basic Information Catalog Number: 20123-1-AP Size: 52 μg/150 μl Source: Rabbit Isotype: IgG Purif ication Method: Antigen af f inity purif ication Immunogen Catalog Number: AG13847 GenBank Accession Number: BC000262 GeneID (NCBI): 79004 Full Name: CUE domain containing 2 Calculated MW: 287aa,32 kDa Observed MW: 32 kDa Recommended Dilutions: WB 1:1000-1:4000 IP 0.5-4.0 ug f or IP and 1:500-1:1000 f or WB IHC 1:400-1:1600 IF 1:50-1:500 Applications Tested Applications: IF, IHC, IP, WB, ELISA Species Specif icity: human,mouse Note-IHC: suggested angen retrieval with TE buffer pH 9.0; (*) Alternavely, angen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : HepG2 cells; human brain tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue, HepG2 cells, Jurkat cells, mouse brain tissue IP : mouse brain tissue; IHC : human ovary tumor tissue; human breast cancer tissue, human malignant melanoma tissue IF : HeLa cells; Background Information CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif -containing protein involved in the regulation of the cell cycle, inf lammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) f or dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription f actor NF-κB. Moreover, CUEDC2, is a key f actor in endocrine resistance in breast cancer. Notable Publications Author Pubmed ID Journal Application Storage Storage: Store at -20ºC. Stable f or one year af ter shipment. Storage Buf f er: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary f or -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll f ree in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data f or this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of Polyclonal ANTIBODY CUEDC2 - ptglab.com filePBS with 0.02% sodium azide and 50% glycerol pH 7.3....
CUEDC2Polyclonal ANTIBODYCatalog Number:20123-1-AP
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:20123-1-APSize:52 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG13847
GenBank Accession Number:BC000262GeneID (NCBI):79004Full Name:CUE domain containing 2Calculated MW:287aa,32 kDaObserved MW:32 kDa
Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:400-1:1600 IF 1:50-1:500
Applications Tested Applications:IF, IHC, IP, WB, ELISASpecies Specif icity:human,mouseNote-IHC: suggested an gen retrieval withTE buffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0
Positive Controls:WB : HepG2 cells; human brain tissue, humankidney tissue, mouse brain tissue, mouse kidneytissue, HepG2 cells, Jurkat cells, mouse braintissueIP : mouse brain tissue;IHC : human ovary tumor tissue; human breastcancer tissue, human malignant melanoma tissueIF : HeLa cells;
Background Information CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif -containing protein involved in the regulation of the cell cycle, inf lammation, and tumorigenesis.It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers,such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation byrecruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrineresistance in breast cancer.
Notable Publications Author Pubmed ID Journal Application
Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (tollf ree in USA), or 1(312) 455-8498 (outsideUSA)
E: [email protected] W: ptglab.com
For technical support and original validation data for thisproduct please contact:
This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 20123-1-AP(CUEDC2 antibody) at dilution of 1:4000incubated at room temperature for 1.5 hours
IP Result of anti-CUEDC2 (IP:20123-1-AP, 4ug;Detection:20123-1-AP 1:500) with mouse braintissue lysate 4000ug.
Immunohistochemical analysis of paraff in-embedded human ovary tumor tissue slide using20123-1-AP (CUEDC2 antibody) at dilution of1:800 (under 10x lens). heat mediated antigenretrieved with Tris-EDTA buffer(pH9).
Immunohistochemical analysis of paraff in-embedded human ovary tumor tissue slide using20123-1-AP (CUEDC2 antibody) at dilution of1:800 (under 40x lens). heat mediated antigenretrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of ( -20°C Ethanol)f ixed HeLa cells using 20123-1-AP (CUEDC2antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Aff iniPure Goat Anti-Rabbit IgG(H+L)
![an Ugi-azide multicomponent reaction Supporting …S1 Supporting information Novel synthesis of lower rim α-hydrazinotetrazolocalix[4]arenes via an Ugi-azide multicomponent reaction](https://static.fdocument.org/doc/165x107/5f3ff21b6dc20e37e43906a6/an-ugi-azide-multicomponent-reaction-supporting-s1-supporting-information-novel.jpg)
