Peptide Mapping Infographic · PDF file Title: Peptide Mapping Infographic Subject:...

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Transcript of Peptide Mapping Infographic · PDF file Title: Peptide Mapping Infographic Subject:...

  • Oxidation of Methionine

    D Y M P K

    O

    N-terminal Glutamine to pyroGlu

    Q V Q L V

    O

    O

    R

    H2N NH

    R + H2O O

    Conjugation sites

    D A C P K

    N-linked

    O-linked

    N Y S T K

    S D Y C K

    Glycosylation

    A C T Y K

    A C D Y K

    Sequence Variance

    D C D Q K

    A Y C S K

    S-S

    Disulfide Bridge location

    P G K

    P G

    C-terminal Lysine clipping

    O

    β

    α

    O

    N H

    N H OH

    isoAspD or

    P E N N Y K

    Deamidation of Asparagine

    Points of interest on a peptide map

    What do we look for when mapping biopharmaceuticals?

    RT

    A

    C

    D

    Y

    M

    D

    K

    A

    C

    D

    Y

    K

    Y

    M

    D

    K

    D

    P

    C

    Y

    K

    C

    D

    K

    Peptide Separation

    m/z

    A

    C

    D

    Y

    M

    D

    K

    A

    C

    D

    Y

    K

    Y

    M

    D

    K

    D

    P

    C

    Y

    K

    C

    D

    K

    Peptide Detection

    A C D Y M D K

    A C D Y K

    Y M D K D P C Y K

    C D K

    N-terminus –

    – C-terminus

    Data Interpretation

    Protein Digestion A C D Y K D P C Y K

    Mapping a biopharmaceutical route

    What are the steps involved in peptide mapping?

    ©2016 Thermo Fisher Scientific. All rights reserved. ST72150-EN 1016S

    Learn more at: thermofisher.com/PeptideMaps

    • Complete and reproducible protein digestion

    • High resolution reproducible peptide separations

    • HRAM MS and MS/MS

    • Powerful yet user-friendly software

    Choosing the correct tools for your journey

    What are the key tools to confidently map a protein?

    • Peptide mapping has the selectivity to simultaneously monitor many attributes or points of interest.

    • This is important for maintaining the safety and efficacy of protein therapeutics

    http://www.thermofisher.com/peptidemaps http://www.thermofisher.com/peptidemaps