Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know...

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Oxidative Phosphorylation Part 2 Chapter 19

Transcript of Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know...

Page 1: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Oxidative Phosphorylation

Part 2

Chapter 19

Page 2: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Oxidative Phosphorylation Part 2

Key Topics: To Know

1.How cells deal with reactive oxygen species (ROS).2.Calculating ΔGo’ of the Proton Motive Force.3.Membrane ATPase and how it works.4.Cytoplasmic NADH getting into the mitochondria.5. Adenylate Control.6. Mitochondia and apoptosis.7.PMF can be used to ?

Page 3: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ROS : Reactive Oxygen Species

Page 4: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ΔG of the Proton Motive Force

Page 5: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Nobel Prize for Chemiosmotic Model = PMF

Page 6: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Converting PMF to ATP

Page 7: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Mitochondrial Respiration+Phosphorylation

EOC Problem 9: Compartmentalization of Citric Acid Cycle.

Page 8: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Adjusting the pH Generates ATP

Page 9: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Molecules that Collapse the PMF

Page 10: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

DNP is an Uncoupler

EOC Problem 6: Figuring out what happens with uncouplers.

Page 11: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Odorous Flowers that Heat Up Using Uncoupled Respiration

Page 12: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Worked out F1 ATPase kinetic mechanism + First purified the FoF1 ATPase

Page 13: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ATP Synthase

Page 14: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ATP Synthase Catalyzes by Steps

Page 15: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

F1 Fo ATP synthase

Page 16: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

F1 Complex

Page 17: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 18: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 19: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Used to Inhibit ATP Synthase Reaction

Page 20: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

F1 ATPase Spinning

Page 21: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 22: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ATP Synthase Seen Rotating !

Page 23: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 24: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 25: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

ATP Synthase Substrate Transport

ADP and Pi are products of Anabolism

EOC Problem 11 is the

effect of ADP and Pi on ATP

Synthesis.

Get Ready, NADH formed in Cytoplasm

in Next

Page 26: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Glycolysis NADH Enters the Mitochondria by the Malate Aspartate Shuttle

Page 27: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

NADH from Glycolysis Can Also Get in by Glycerol Phosphate DH

Also has a Anabolic Role

Page 28: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

EOC Problems 13 and 14: Get in to the Pasteur Effect and “Petites” in Yeast Colonies. Fun stuff.

Page 29: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Adenylate Control of Glycolysis/CAC/e-transport

1. Acceptor Control: availability of ADP + Pi

2. Mass action ratio: [ATP]/([ADP] [Pi])

EOC Problem 17 Gets into the rate of ATP turnover in heart muscle…the muscle that always has to be “on”.

EOC Problem 18 Gets into the same in insect flight muscle.

Page 30: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Inhibitory Protein IF1 Stops Loss of ATP During Ischemia that is Hypoxia

IF (red and white) forms dimers at pH 6.5 to Stop Rotation of ATP Synthase

Page 31: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Thermogenin – Protein Uncoupler in Brown Fat

Page 32: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Mitochondria in Adrenal Gland: P-450 Oxygenases Specialized for Steroid Synthesis

Page 33: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Electron Flow to P-450

Page 34: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Apoptosis is Regulated by the

Mitochondria

Initiates a series of proteasess

Stress, Damage

EOC Problem 19: relates mitochondrial function to cancer. Hint think about the first couple of slides.

Page 35: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).
Page 36: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Escherichia coli Electron Transport

Page 37: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Bacterial Quinone

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Bacteria Use the PMF to Rotate Their Flagella

Page 39: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Proton Motive Force Functions To

1. Synthesize ATP (from ADP + Pi).

2. Active Transport (Symports, Antiports, Uniports; review Chapter 11).

3. Rotate Bacterial Flagella.

4. Reversed Electron Transport (some Photosynthetic and Chemoautotrophic Bacteria)

Page 40: Oxidative Phosphorylation Part 2 Chapter 19. Oxidative Phosphorylation Part 2 Key Topics: To Know 1.How cells deal with reactive oxygen species (ROS).

Things to Know and Do Before Class1. How cells deal with reactive oxygen

species (ROS).

2. Calculating ΔGo’ of the Proton Motive Force.

3. Membrane ATPase and how it works.

4. Cytoplasmic NADH getting into the mitochondria.

5. Adenylate Control.

6. Mitochondria and apoptosis.

7. PMF can be used to ?

8. EOC Problems: 6, 9, 11,13,14, 17, 18, 19.