Novel Ion Mobility Technology for LC/MS · PDF file• Custom reporting to comprehensively...

Click here to load reader

  • date post

    12-Mar-2018
  • Category

    Documents

  • view

    221
  • download

    3

Embed Size (px)

Transcript of Novel Ion Mobility Technology for LC/MS · PDF file• Custom reporting to comprehensively...

  • Novel Ion Mobility Technology for LC/MS

    Jaume C. Morales

    MS Product Specialist

    February 26, 2014

    1

  • IMS QTOF - Overview

    UHPLC

    HD QTOF

    IMS Background

    Ion Mobility Basics

    Instrument & Software Overview

    Applications

    - Software tools

    -

    - Lipids

    - Carbohydrates

    Summary

    February 26, 2014

    2

    ASMS 2013 Ion Mobility

    Abstracts

  • IMS QTOF - Overview

    UHPLC

    HD QTOF

    IMS Background

    Ion Mobility Basics

    Instrument & Software Overview

    Applications

    - Software tools

    -

    - Lipids

    - Carbohydrates

    Summary

    February 26, 2014

    3

    ASMS 2013 Ion Mobility

    Abstracts

  • Nueva serie

    LC Agilent

    1200 Infinity

    Page 4

    Presentacin de los

    instrumentos LC de la nueva

    plataforma

    Agilent 1220, 1260 y 1290

  • Page 5

    Nueva Serie Agilent 1200 Infinity Elevando el Estandard en HPLC UHPLC para el Laboratorio

    1260

    Infinity LC

    Modular

    600 bar

    80 Hz

    2x 10x Sensivilidad UV

    100% HPLC Compatible

    Precio HPLC

    Capacidades UHPLC

    1220

    Infinity LC

    Integrado

    600 bar

    80 Hz

    El ms econmico

    HPLC y UHPLC

    Tecnologia 1260/1290

    1290

    Infinity LC

    Modular

    1200 bar

    160 Hz

    Mximo rendimiento UHPLC

    Mxima Flexibilidad

    Coste Servicio HPLC

  • Page 7

    Que es UHPLC ?

    1. Las partculas ms pequeas permiten una menor altura de

    plato terico mostrando pues una mayor resolucin

    cromatogrfica.

    2. La resolucin cromatogrfica sufre menos al incrementar el

    flujo cuanto ms pequea es la partcula

    Caractersticas de UHPLC

    Resolucin y Flujo

  • Page 8

    Nueva Serie Agilent 1200 Infinity Elevando el Estandard en HPLC UHPLC para el Laboratorio

    1260

    Infinity LC

    Modular

    600 bar

    80 Hz

    2x 10x Sensivilidad UV

    100% HPLC Compatible

    Precio HPLC

    Capacidades UHPLC

    1220

    Infinity LC

    Integrado

    600 bar

    80 Hz

    El ms econmico

    HPLC y UHPLC

    Tecnologia 1260/1290

    1290

    Infinity LC

    Modular

    1200 bar

    160 Hz

    Mximo rendimiento UHPLC

    Mxima Flexibilidad

    Coste Servicio HPLC

  • Page 9

    Nueva Serie Agilent 1200 Infinity - Rangos de Potencia

    0

    200

    400

    600

    800

    1000

    1200

    0 2 4 6 8 10

    Toda Columna analtica

    Transferencia de mtodo

    Mayor Resolucin

    Anlisis ms rpidos

    Disolventes ms viscosos

    Bajas Temperaturas

    Semi-preparativo

    1290 Infinity LC

    1260 Infinity Quat./Iso LC

    1220 Infinity LC

    Presin (bar)

    Flujo (ml/min)

    1260 Infinity Binary LC

    Un nuevo rango de potencias capaz de proporcionar el mximo

    rendimiento, flexibilidad, compatibilidad y proteccin de la inversin

  • NUEVA Serie 1200 Infinity Mayor automatizacin y enfoque en la aplicacin

    GPC

    SEC

    Preparative

    LC & LC/MS

    Nanoflow

    HPLC-Chip MS

    Isocratic

    LC

    Nano LC

    & Cap LC

    Analytical

    SFC

    Bio-inert

    HPLC

    Multi-method and

    Method Development

    High-throughput

    LC & LC/MS

    Page 10

  • IMS QTOF - Overview

    UHPLC

    HD QTOF

    IMS Background

    Ion Mobility Basics

    Instrument & Software Overview

    Applications

    Software tools

    Lipids

    Carbohydrates

    Summary

    ASMS 2013 Ion Mobility

    Abstracts

  • Agilent 6000 Series LC/MS Portfolio

    6200 series TOF

    6100 series Quad

    6500 series Q-TOF

    6400 series QQQ

    High Resolution - Qualitative

    Low Resolution - Quantitative

    Sin

    gle

    Stag

    e Tan

    dem

    Stage

  • 6400 Triple Quadrupole Mass Spectrometer Feature Benefit

    Dynamic MRM Extremely complex samples

    Jet Stream Technology High sensitivity

    Full autotune and calibration Easy to maintain

    Broadest range of ion sources Multiple applications

    Wide dynamic range Complex sample analysis

    Fast polarity switching Analysis of multiple sample types

    Dual Ion Funnel Highest sensitivity

    6460

    6420

    Focused analysis of small molecules e.g., organic chemistry Natural product research Pharmaceutical analysis such as BA/BE DMPK studies Quantitative analysis of compounds in high complexity backgrounds Quantitative food safety analysis e.g., pesticides, drug residues Clinical analysis e.g., endocrinology, new born screening Peptide and metabolite quantitation

    6430

    6490

  • 6500 True High Def Q-TOF Mass Spectrometer Feature Benefit

    True High-Def TOF High mass accuracy

    Jet Stream Technology High sensitivity

    Full autotune and calibration Easy to maintain

    Broadest range of ion sources Multiple applications

    Wide dynamic range Complex sample analysis

    ChipLC Superb nano-spray applications

    Dual Ion Funnel Highest sensitivity

    6530

    6540

    Proteomics including profiling and differential expression analysis Protein identification amino acid sequencing Metabolomics profiling and identification Quantitative analysis of compounds in low complexity backgrounds Food safety analysis e.g., screening Natural product analysis

    6550

  • Agilent 6550 Q-TOF with iFunnel Technologies

    New- Ion Beam

    Compression and

    Shaping (resolution

    + sensitivity)

    New- iFunnel (10X sensitivity gain)

    Hexapole axial focusing collision cell

    (faster MS/MS spectra)

    New- Dual Agilent

    JetStream orthogonal

    spray (robust and

    stable mass

    calibration)

    4 GHz digitizer + ADC

    detector

    (resolution, mass accuracy,

    dynamic range)

    Longer Invar flight tube

    (resolution + stable mass

    accuracy)

    New embedded processor (50 spectra/sec)

    March 2, 2012 15

  • 6550 iFunnel Q-TOF

    LC/MS System

    ASMS 2010

    Sensitivity

    Dramatically improved quantitative capabilities

    New Qual/Quan Workflows

    Superior metabolite and protein detection

    Non-targeted compound screening

    Comprehensive Performance Enhancements

    Mass Resolution >40,000

    50 spectra /sec MS and 33 spectra/sec MS/MS

    5 orders of linear dynamic range

  • iFunnel Technology Revolutionizes Ion Sampling

    10X Sensitivity Breakthrough Proven on 6490 QQQ

    Agilent Jet

    Stream

    Hexabore

    Capillary Dual Stage Ion Funnel

    Now- 10X Sensitivity Breakthrough for 6550 QTOF

  • iFunnel Technology Revolutionizes Ion Sampling

    Agilent Jet Stream

    Thermal confinement of

    ESI plume

    Efficient desolvation to

    create gas phase ions

    Creates an ion rich zone

    Hexabore Capillary

    6 capillary inlets

    Samples 6 times more ion

    rich gas from the source

    Captures the majority of

    the gas from the source

    region

    Dual Ion Funnel

    Removes the gas but

    captures the ions

    Removes neutral noise

    Extends turbo pump life

  • 2. Instrument and Software Overview

    Start temperature 250 C at t = 0 min

    Focusing of the ion beam

  • 2. Instrument and Software Overview

    Focusing of the ion beam

    2R

    Stop Temperature 4000 C at t = 8 min

  • QTOF Acquisition MS and MS/MS Modes of Operation

    MS Only TOF only mode

    MS/MS All Ions . MS & MS/MS info at the same time.

    Data Dependent MS/MS Experiments

    Precursor selection based on intensity of n-highest (with relative and absolute threshold)

    Excluded and Preferred mass lists

    Configurable charge-state selection preference

    Data Directed (Targeted) MS/MS experiments

    Import of target mass lists from Mass Profiler or Mass Profiler Professional software

    Import of mass lists from other applications

    Automatic dynamic creation of time segments

    2. Instrument and Software Overview

  • Screening and identification workflow

    Agilents approach

    Combination of UHPLC separation and accurate mass TOF technology

    Effective data mining algorithms to FIND compounds in a sample

    Optional software to COMPARE samples or sample sets to identify differences

    AMRT Databases and MS/MS Library Search to easily IDENTIFY targeted compounds

    Several algorithms to help IDENTIFYING unknown compounds (MFG, MSC)

    User Interface to easily NAVIGATE RESULTS

    Custom reporting to comprehensively REPORT results

    Full AUTOMATION of data acquisition, processing and reporting

    TOF/

    Q-TOF

    Analysis

    ID via

    AMRT

    DBs

    Find

    Compounds

    MFG of

    Compounds

    w/ MSMS

    MSMS

    structural

    correlation

    ID via

    MSMS

    libraries

    OPTION:

    Differential

    Analysis

    Print

    custom

    report

    Automation

  • Screening and identification workflow

    TOF/

    Q-TOF

    Analysis

    ID via

    AMRT

    DBs

    Molecular

    Feature

    Finding

    MFG of