MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA...

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A DSIR approved R&D Facility MOLECULAR BIOLOGY gccbiotech.co.in GCCBiotech(India)Pvt.Ltd. [email protected] [email protected] [email protected] 91-33-24951044/24950004 Joychandipur,Bakhrahat,PIN.743377, South24Parganas,W.B.,INDIA

Transcript of MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA...

Page 1: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

A DSIR approved R&D Facility

MOLECULAR BIOLOGY

�gccbiotech.co.in�

GCC�Biotech�(India)�Pvt.�Ltd.

[email protected]@[email protected]

�91-33-24951044�/�24950004

Joychandipur,Bakhrahat,�PIN.743377,South�24�Parganas,�W.B.,�INDIA

Page 2: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Super DH 5α Competent Cell

Super XL-1 Blue Competent Cells

Efficomp supercompetent E.coli cells

BL21(DE3) Competent E. coli

BL21(DE3)pLysS Competent Cells

X-Gal solution, 20mg/ml, Ready to use

100mM IPTG solution, Ready to use

X-Gal

IPTG, Dioxane-Free

MOLECULAR Biology

DNA template

(cDNA/gDNA)

Primers/oligos

G9 Taq DNA polymerase

Hi- G9 Taq DNA polymerase

2x G9 Taq PCR Master Mix

2x Hi G9 Taq PCR Master Mix

PrimeTaq DNA Polymerase

Hi-PrimeTaq DNA Polymerase

G9 PCR CORE KIT (G9 Taq)

2x PrimeTaq PCR Master Mix

2x Hi-PrimeTaq PCR Master Mix

Hot Start G9 Taq DNA Polymerase

2X Hot Start G9 Taq DNA Polymerase

Hot Start PrimeTaq DNA Polymerase

2X Hot Start PrimeTaq DNA Polymerase

GPfu DNA Polymerase

2x GPfu Master Mix

Pro Pfu DNA Polymerase (Pfu Turbo)

2x Pro Pfu (Pfu Turbo)Master Mix

DeltaQ Polymerase

dNTP's (100mM dNTP set)

10mM dNTP mix

PCR grade extra pure DMSO

Real time PCR

analysis

2x qPCR mastermix, SYBR, Rox

2X H-eff qPCR master mix, Rox

WRTaqman 2X mastermix

FasTaqman mastermix for Blood

Taqman probes

PCR amplificationAnalysis on

agarose gel

DNA purification

DNA modification

and LigationDNA loading dye

10X TBE Buffer -HI-grade

10X TAE Buffer -HI-grade

High Gel Agarose, Molecular biology grade

Ethidium Bromide solution (10mg/ml)

Ethidium Bromide-Blue solution (10mg/ml)

Super Stain Nucleic Acid Gel Stain -

Ultra (100000x)

DNA Ladder

Bacterial

transformation

T4 Polynucleotide Kinase, Hi-Q

Klenow Fragment

Shrimp Alkaline Phosphatase (SAP)

Alkaline Phosphatase Calf Intestinal (Hi-Q)

T4 DNA Ligase

Hi efficiency TA-Cloning kit

Screening of

clones

Isolation and

purification of DNA

Biochemical

characterisationSite-directed

mutagenesis

Sequence

identification

Protein purificationBacterial transformation/

protein overexpressionGLyseB (Bacterial lysis buffer)

2X Lysis/ equlibration buffer

His Protein Purification-Wash Buffer

Lysozyme (10 mg/ml) in 10 mM

Tris-HCl, pH 8.0

Hi- bind Ni-NTA Agarose Bead

Hi- bind Ni-IDA Agarose Bead

Site Directed Mutagenesis Kit S (6Kb)

Site Directed Mutagenesis Kit L (10Kb)

Pro Pfu DNA Polymerase (Pfu Turbo)

DpnI

Efficomp supercompetent E.coli cells

Gsure® plasmid Mini kit

GSure® Splash Plasmid mini kit

GSure® Rapid plasmid Mini kit

1X Colony PCR Master Mix

1X Colony PCR Mix (T7)

1X Colony PCR Mix (M13)

GSure®Splash Plasmid mini kit

Gsure® PCR Purification Kit

GSure® Gel extraction kit

Page 3: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

GSure® Ultra kit is highly efficient to deliver genomic DNA and

total RNA from same sample. The Kit works robustly on wide

range of samples like Plant tissue, animal tissue, cultured cells,

blood and bacteria. This kit provides simple, rapid and

convenient technique to isolate DNA and RNA of high yield from

very small sample volume. The kit is equally efficient on fresh as

well as stored sample in RNA Later.

Different kit is designed for efficient lysis, removal of

contaminants and purification of superior grade nucleic acids

from bacteria, plant, blood, cultured cells and tissue samples.

Buffers of different isolation kits are uniquely formulated for

rapid and efficient lysis of that particular cell type.

GSure® Ultra Nucleic Acid isolation kit delivers total nucleic

acid population of a particular sample, thus makes the

transcriptome analysis more perfect in reference to the genome

content.

GSure® ULTRA kit combines the advantages of a silica-based

microspin column for efficient binding and purity of the

extracted nucleic acid. The kit is a dual column based isolation kit

for selective binding of DNA and RNA with the respective

column.

Purified DNA and RNA are compatible for all sorts of down-

stream application. Purified genomic DNA is suitable for PCR

amplification, Southern blotting, restriction digestions, next

generation sequencing and purified RNA is compatible for RT-

PCR amplification, Northern blotting, RNase Protection assay,

microarray etc.

Description

GSure® Ultra Kit

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GSure® Ultra Nucleic Acid Isolation Kit (Bacteria)

Description

GSure® Ultra Nucleic acid isolation kit (Bacteria) is highly

efficient and specific for isolation of total RNA and DNA from

same bacterial sample. Purified DNA and RNA are free from

contamination and are suitable for down-stream application.

This kit provides a simple and convenient technique to isolate

DNA and RNA of high yield with very small sample volume.

GSure® kit combines the advantages of a silica-based system

with a microspin format, thus eliminates the requirement of

expensive resins and hazardous organic compounds.

Buffers provided with the GSure® Ultra Nucleic acid isolation

kit for bacteria assure complete lysis of bacterial cell wall with

digestion of cellular proteins. Buffer composition is optimized in

such a way that DNA binds selectively to GMini DNA Binding

Spin column and RNA binds selectively to the GMini CHROME

column. The lysis buffer contains Proteinase K thus its external

addition is not required. All the buffers are room temperature

comfortable.

Advantages

ü Highly Purified DNA and RNA from same Bacterial culture

just in 45 min. For purification of genomic DNA and total

RNA from 500 µl bacterial cells.

ü An easy to use silica membrane, spin-mini-column based

DNA-RNA isolation kit for rapid, robust and reproducible

isolation of genomic DNA and total RNA from same

samples.

ü Required sample volume is less and has a easy workflow.

ü Delivers high quality integrated RNA in every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Wide range of starting cell volume.

Ordering Information

Featuresæ Easy – spin column format.

æ Convenient-Same optimized protocol for different sources

of sample.

æ High yield- Recovers 2 µg total gDNA and 5 µg total RNA

from 500 µl bacterial cells.

æ Reproducible-Delivers almost equal amount of yield on

every isolation.

æ Easy to use- No requirement of addition of Proteinase K

externally.

æ Cost effective – More preps for the money.

æ Eco-Friendly- Minimum number of steps, thus minimum

number of plasticware required.

Fig. 1:

DNA and RNA were isolated from different bacterial species. 500 ml of 0.6

O.D cells were harvested and total nucleic acid was isolated. 1/10th

volume of isolated DNA and RNA samples were run on 1% agarose TAE

gel.

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Cat. # Product Pack Size

GRD1001BGSure® Ultra Nucleic Acid

Isolation kit (Bacteria)20 prep

GRD1001GSure® Ultra Nucleic Acid

Isolation kit (Bacteria)50 prep

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GSure® Ultra Nucleic Acid Isolation Kit (Blood)

Description

GSure® Ultra Nucleic acid isolation kit (Blood) is highly efficient

to lyse WBCs and extract extremely purified genomic DNA and

total RNA from Blood sample compatible for all sorts of

downstream applications like PCR amplification, southern

blotting, restriction digestions, next generation sequencing for

isolated DNA and RT-PCR amplification, northern blotting, one

step qRT PCR analysis etc for RNA. Only 200 µl of blood from a

helthy individual is sufficient to isolate 2 µg total gDNA and 0.7

µg total RNA. The kit is so robust that it can isolate DNA and RNA

even from 48 hours properly stored blood. Minimum sample

volume required for isolation is 200 µl and isolation steps are

simple and easy.

The kit comes with RBC Lysis Buffer (RBCL Buffer) to lyse RBC

selectively. The kit can also isolate DNA and RNA from small

volume of total blood samples as well as from buffy coat too.

Advantages

ü Highly Purified DNA and RNA from Blood in 45 min.

ü For purification of genomic DNA and total RNA from

200µl to 1ml Fresh Blood.

ü An easy to use silica membrane, spin-mini-column based

DNA-RNA isolation kit for rapid, robust and reproducible

isolation of genomic DNA and total RNA from same

samples.

ü Required sample volume is less and easy workflow.

ü Equally efficient on fresh as well as stored Blood (up to 48

hours at 4˚C).

ü Delivers high quality integrated RNA in every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA ?

ü Wide range of starting volume.

Ordering Information

Features

æ Easy – spin column format

æ Convenient- Same optimized protocol for

different sources of sample

æ High yield- Recovers 2 µg total gDNA and 0.7 µg total

RNA from 200 µl Fresh Blood sample

æ Reproducible- Delivers almost equal amount of yieldon

every isolation

æ Easy to use- No requirement of addition of Proteinase K

externally

æ Cost effective – More preps for the money

æ EcoFriendly- Minimum number of steps, thus minimum

number of plasticware required

Fig. 1.

Total nucleic acid was isolated from 4 different individual (S1-S4).

DNA and RNA were extracted from 1ml of bood. 1/10th volume of

isolated DNA and RNA were run in 1% agarose gel

DNA RNA

S1 S2 S3 S4 S1 S2 S3 S4

Cat. # Product Pack Size

GRD1003BGSure® Ultra Nucleic Acid

Isolation Kit (Blood)20 prep

GRD1003 GSure® Ultra Nucleic Acid

Isolation Kit (Blood)

50 prep

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GSure® Ultra Nucleic Acid Isolation Kit (Tissue)

Description

Proper lysis of tissue is the step to isolate good quantity and

quality gDNA and RNA. GSure® Ultra Nucleic acid isolation kit

(Tissue) contains 3 lysis buffers which lyse the tissue samples as

fast and efficient as possible. For complete isolation of DNA and

RNA from tissue, only 15 min incubation of the sample in lysis

buffer is enough. The kit is highly efficient to deliver total RNA

from animal tissue viz. organs, muscle, skin or even from tail.

Optimized protocol guaranties extremely purified DNA and

RNA from same sample. GSure® Ultra Nucleic acid isolation kit

(Tissue) is so robust that it can isolate DNA and RNA from any

type of tissue samples ensuring same yield every time. Proper

sample preparation is vital to obtain high yield of DNA and RNA.

Advantages

ü Highly Purified DNA and RNA from Tissue Just in 45 min.

ü For purification of genomic DNA and total RNA from

animal tissue viz. organs,

ü muscle, skin.

ü An easy to use silica membrane, spin-mini-column based

DNA-RNA isolation kit

ü for rapid, robust and reproducible isolation of genomic

DNA and total RNA from same samples.

ü Required sample volume is less and easy workflow.

ü Equally efficient on fresh as well as stored tissue sample in

NA Later

ü Delivers high quality integrated RNA in every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

Ordering Information

Features

æ Easy – spin column format.

æ Convenient- Same optimized protocol for different

sources of sample.

æ High yield- Recovers 2 µg total gDNA and 4µg total RNA

from 10 mg mice brain.

æ Reproducible-Delivers almost equal amount of yield on

every isolation.

æ Easy to use- No requirement of addition of Proteinase K

externally.

æ Cost effective – More preps for the money.

æ EcoFriendly- Minimum number of steps, thus minimum

number of plasticware required.

Fig. 1.

Isolation of DNA and RNA from different tissue of mice using GSure®

Ulra Nucleic acid Isolation Kit for Tissue: DNA and RNA were isolated

from 10 mg of corresponding tissue samples of Mice. 1/10th volume of

isolated nucleic acid (DNA and RNA) were analyzed in 1% agarose gel.

DNA RNA

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Cat. # Product Pack Size

GRD1005B GSure® Ultra Nucleic Acid

Isolation Kit (Tissue)20 prep

GRD1005 GSure® Ultra Nucleic Acid

Isolation Kit (Tissue)

50 prep

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GSure® Ultra Nucleic Acid Isolation Kit (Cultured cells)

Description

Gsure® Ultra Nucleic acid isolation kit (Cultured Cell) is

designed and optimized to isolate gDNA and total RNA from

cultured cells. The kit works equally efficient on adherent as well

as suspension cells. Isolated RNA is remarkably enriched with

small RNA. This kit can isolate nucleic acids from a wide range of

sample volume (10,000 cells to 6 million cells). Lysis buffers is

composed of Proteinase K, so external addition of the enzyme is

not required. Isolated DNA and RNA are compatible for all sorts

of downstream applications like restriction digestion, PCR,

Southern blotting or next generation sequencing with the

isolated DNA. Northern blotting experiments, RT-PCR, RNase

Protection Assay or the micro array could be done with the

isolated RNA.

Advantages

ü Highly Purified DNA and RNA from cultured Cell Just in

45 min.

ü For purification of genomic DNA and total RNA from 6

million cultured cells.

ü An easy to use silica membrane, spin-mini-column based

DNA-RNA isolation kit for rapid, robust and reproducible

isolation of genomic DNA and total RNA fro same

samples.

ü Required sample volume is less and easy workflow.

ü Equally efficient on different cell lines.

ü Delivers high quality integrated RNA in every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

Ordering Information

Features

æ Fast – less than one hour required

æ Easy – spin column format

æ Convenient- Same optimized protocol for different

sources of sample

æ High yield- Recovers 10µg total gDNA and 45µg total

RNA from 6 million Cultured Cell

æ Reproducible- Delivers almost equal amount of yieldon

every isolation

æ Easy to use- No requirement of addition of Proteinase K

externally

æ Cost effective – More preps for the money

æ EcoFriendly- Minimum number of steps, thus minimum

number of plasticware required

Fig. 1:

DNA and RNA were isolated from different cell lines. 10% volume of

isolated nucleic asids were run on 1% agarose gel.

DNA RNA

Cat. # Product Pack Size

GRD1002B GSure® Ultra Nucleic Acid

Isolation Kit (Cell Culture)20 prep

GRD1002 GSure® Ultra Nucleic Acid

Isolation Kit (Cell Culture)

50 prep

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Description

Plant cells contain cell wall which is very hard to break and

isolation of gDNA and RNA from plant tissues is always a great

challenge. Usually plant tissues are crushed with liquid nitrogen

followed by isolation procedure. GSure® Ultra Nucleic acid

isolation kit (Plant) comes with a pre patented unique and

efficient buffer composition which does not require the use of

liquid nitrogen prior isolation, thus making the kit more useful.

GSure® Ultra Nucleic acid isolation kit (Plant) is highly efficient

to deliver total RNA from wide range of samples like Plant Root,

leaf, Seed, Flower and Fruit. Purified DNA and RNA are free from

any sort of secondary metabolites and are compatible for all

kind of downstream applications. Sample requirement of the kit

is only 25�mg for leaf samples and the yield of DNA is not less

than 2.5 mg for DNA and 10mg for RNA.

Advantages

ü Highly Purified DNA and RNA from any sample Just in 45

min.

ü For purification of genomic DNA and total RNA from Plant

tissue, animal tissue,cultured cells, blood and bacteria.

ü An easy to use silica membrane, spin-mini-column based

DNA-RNA isolation kit

ü for rapid, robust and reproducible isolation of genomic

DNA and total RNA from same samples.

ü Required sample volume is less and easy workflow.

ü Equally efficient on fresh as well as stored sample in RNA

Later.

ü Delivers high quality integrated RNA in every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

Fig. 1.

Isolation of DNA and RNA were isolated from 5 different plant leafs

(P1-P5) (25mg each) electrophoresed on 1% Agarose gel.

Features

æ Fast – less than one hour required.

æ Easy – spin column format.

æ Convenient- Same optimized protocol for different

sources of sample.

æ High yield- Recovers 2 µg total gDNA and 2 µg total RNA

from 25 mg Plant Leaf Sample.

æ Reproducible-Delivers almost equal amount of yield on

every isolation.

æ Easy to use- No requirement of addition of Proteinase K

externally.

æ Cost effective – More preps for the money.

æ EcoFriendly- Minimum number of steps, thus minimum

number of plasticware required.

Fig. 2.

DNA and RNA were isolated from 100mg of stored sapota seed.

1/10th volume of the eluted DNA and RNA were fractionated on 1%

agarose gel.

Ordering Information

GSure® Ultra Nucleic Acid Isolation Kit (Plant)

DN

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Cat. # Product Pack Size

GRD1004BGSure® Ultra Nucleic Acid

Isolation Kit (Plant)20 prep

GRD1004 GSure® Ultra Nucleic Acid

Isolation Kit (Plant)

50 prep

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GSure® Dogma kit is highly efficient to deliver high amount of

genomic DNA, RNA and protein from same sample. This kit

provides a simple and convenient technique to isolate all three

biomolecules from wide range of samples like Plant tissue,

animal tissue, cultured cells, blood and bacteria. Time required

for isolation of all three bio-molecules is just one hour. The kit

can isolate all three biomolecules from same sample, thus it

allows the researchers for simultaneous analysis of genomics,

transcriptomics and proteomics of a particular sample. Isolation

of DNA, RNA and Protein from same source minimizes the

experimental error and makes the data more scientific. On one

hand, the kit is cost-effective as it offers more preps for money

and on the other hand, it is eco-friendly since it minimizes the

use of plastic wares. GSure® Dogma Kit comes with such buffer

composition that isolation protocol for different sources are

same. Sample Volume required for isolation is also minimal and

isolation steps are simple and easy.

Gsure® Dogma kit combines the advantages of a silica-based

system with a microspin format, thus eliminates the requirement

of expensive resins and hazardous organic compounds. GSure

Dogma kit comes with Gmini DNA Spin column which is

specially designed to bind genomic DNA. This kit also contains

GMini CHROME column which is specialized for binding total

RNA selectively. The DNA spin columns as well as the Chrome

columns retain minimum amount alcohol from wash buffer;

hence delivers purified form of genomic DNA and total RNA

every time after elution.

Buffers provided with the GSure Dogma Kit assure complete

lysis of cells without digestion of cellular proteins. Buffer

composition is optimized in such a way that DNA binds

selectively to GMini Spin column and RNA binds selectively to

the GMini CHROME column whereas the total protein comes in

the flow-through. Dogma kit also comes with a unique Protein

Precipitation Cocktail for rapid precipitation of total protein

from the flow-through. Protein pellet wash buffer ensures

complete removal of contaminants and delivery of purified

protein pellet every time. Protein pellet resuspension solution

ensures complete dissolution of pellet.

Purified genomic DNA is compatible for all sorts of downstream

applications like PCR amplification, southern blotting,

restriction digestions, next generation sequencing etc. Purified

RNA is compatible for RT-PCR amplification, northern blotting,

RNase Protection Assay, Microarray etc. Purified protein is

compatible for total protein profiling, western blotting etc. ern

blotting, RNase Protection assay and microarray etc.

Description

GSure® Dogma Kit

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Advantages

ü Complete Isolation from Same Sample just in 1 Hr.

ü Fast and efficient purification of DNA, RNA and Protein

from 500 µl bacterial cells.

ü An easy to use silica membrane, spin-mini-column based

isolation kit for rapid, robust and reproducible isolation of

all three bio-molecules.

ü Required sample volume is less and easy workflow.

ü Delivers high quality integrated DNA, RNA and Protein in

every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Qualitatively and quantitatively better than any Phase

Separation Method

Fig.1.

Over expression followed by isolation of DNA, RNA and Protein from

same sample at different time points.

Fig.2.

RT PCR was performed RNA on total was RNA from H2BpET28b

transformed BL21DE3, RNA was harvested before and after isolation.

Features

æ Efficient- DNA, RNA and Protein from same sample.

æ Fast– One hour required to isolate 3 bio- molecules.

æ Convenient- Same optimized protocol for different

sources of sample

æ High yield- Recovers 5 µg DNA, 25 µg RNA and 50 µg

Protein from 500 µl bacterial cells.

æ User Friendly– Less sample volume and easy

æ workflow.

æ Reproducible- Delivers almost equal amount of

æ yield on every isolation.

æ Easy– spin column format.

æ Cost effective- More preps for the money.

æ Eco-Friendly- Minimum number of steps, thus

æ minimum number of plastic ware required.

Fig. 3.

Bar Plot showing expression profile of H2B protein at different time

after induction with IPTG. Expression profiling was performed at RNA

level by RT PCR and at protein level by target band quantification.

Ordering Information

GSure® Dogma Kit for Bacteria

Cat # Product Pack Size

GD1001AGSure® Dogma Kit for

Bacteria20 prep

GD1001GSure® Dogma Kit for

Bacteria50 prep

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Advantagesü Complete Isolation from Plant Sample just in 1 Hr.

ü Fast and efficient purification of DNA, RNA and Protein

from Plant parts viz.

ü Root, leaf, Seed, Flower and Fruit.

ü An easy to use silica membrane, spin-mini-column based

isolation kit for rapid.

ü robust and reproducible isolation of all three bio-

molecules.

ü Required sample volume is less and easy workflow.

ü Delivers high quality integrated DNA, RNA and Protein in

every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Qualitatively and quantitatively better than any Phase

Separation Method.

Fig.1.

DNA, RNA and protein were isolated from 5 differentaged Colocasia

leafs (25mg). 10% of the isolated biomolecules were electrophoresed on

gel.

Featuresæ Efficient- DNA, RNA and Protein from same sample.

æ Fast– One hour required to isolate 3 bio-molecules.

æ Convenient- Same optimized protocol for different

sources of sample

æ High yield- Recovers 3µg DNA, 3µg RNA and 50µg

Protein from 25mg plant leaf.

æ User Friendly– Less sample volume and easyworkflow.

æ Reproducible- Delivers almost equal amount of yield on

every isolation.

æ Easy – spin column format.

æ Cost effective – More preps for the money.

æ Eco-Friendly- Minimum number of steps, thus minimum

number of plasticware required.

Ordering Information

GSure® Dogma Kit for Plant

Cat. # Product Pack Size

GD1002A GSure® Dogma Kit for Plant 20 prep

GD1002 GSure® Dogma Kit for Plant 50 prep

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Ultrapure Oligonucleotides

GCC provides a full spectrum of highquality oligonucleotides modifications,with various synthesis scales and applications to ensure consistentlyfast turnaround time.

Ultrapure Oligonucleotides

GCC provides a full spectrum of highquality oligonucleotides modifications,with various synthesis scales and applications to ensure consistentlyfast turnaround time.

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Advantageü Complete Isolation from Cultured Cell just in 1 Hr.

ü Fast and efficient purification of DNA, RNA and Protein

from 1 million cultured cells.

ü An easy to use silica membrane, spin-mini-column based

isolation kit for rapid,robust and reproducible isolation of

all three bio-molecules.

ü Required sample volume is less and easy workflow.

ü Delivers high quality integrated DNA, RNA and Protein in

every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Qualitatively and quantitatively better than any Phase

Separation Method.

Fig. 1.

DNA, RNA and Protein were isolated from different cultured cell line.

DNA and RNA were run on 1% agarose TAE gel. l/10th volume of isolated

biomolecules were fractionated on gel.

Featuresæ Efficient- DNA, RNA and Protein from same sample.

æ Fast– One hour required to isolate 3 bio-molecules.

æ Convenient- Same optimized protocol fordifferent sources

of sample

æ High yield- Recovers 5 µg DNA, 25 µg RNA and 50 µg

Protein from 1 million cultured cells.

æ User Friendly– Less sample volume and easy workflow.

æ Reproducible- Delivers almost equal amount of

æ yield on every isolation.

æ Easy– spin column format.

æ Cost effective– More preps for the money.

æ Eco-Friendly- Minimum number of steps, thus minimum

number of plasticware required.

Ordering Information

GSure® Dogma Kit for Cultured Cell

Cat. # Product Pack Size

GD1003AGSure® Dogma Kit for Cultured Cell

20 prep

GD1003 Gsure® Dogma Kit for Cultured Cell 50 prep

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Tailored to Meet Your Needs....

GCC’s popular protein biology products

Protein isolation from bacteria,

culture cell, animal and plant tissue

GQuant Bradford Reagent for

colorimetric protein assay

Gbond Ni-IDA Agarose bead

for protein purification

Simple, sensitive and rapid

detection of protein with SARP Stain Protein Dye

Explore

Protein

ScienceExplore

Protein

Science

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GSure® Dogma Kit for Tissue

Advantages

ü Complete Isolation from Tissue Sample just in 1 Hr.

ü Fast and efficient purification of DNA, RNA and Protein

from Plant parts viz. Root, Leaf, Seed, Flower and Fruit.

ü An easy to use silica membrane, spin-mini-column based

isolation kit for rapid, robust and reproducible isolation of

all three bio-molecules.

ü Required sample volume is less and easy workflow.

ü Delivers high quality integrated DNA, RNA and Protein in

every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Qualitatively and quantitatively better than any Phase

Separation Method.

Fig. 1:

Isolation of DNA, RNA and Protein from different tissue of mice using

GSure® Dogma Kit for Tissue: DNA, RNA and Protein were isolated from

lOmg of corresponding tissue samples of Mice. Mice was sacrifised and

the organs were collected in RNA Later. Biomolecules were extracted

after 1 week of tissue collection. l/10th volume of isolated nucleic acid

(DNA and RNA) and protein were fractionated on 1% agarose TAE and

12% SDS-Polyacrylamide gel respectively.

Features

æ Efficient- DNA, RNA and Protein from same sample.

æ Fast– One hour required to isolate 3 bio-molecules.

æ Convenient- Same optimized protocol for different

sources of sample

æ High yield- Recovers 3 µg DNA, 3 µg RNA and 50 µg

Protein from 10 mg mice brain.

æ User Friendly– Less sample volume and easy workflow.

æ Reproducible- Delivers almost equal amount of yield on

every isolation.

æ Easy– spin column format.

æ Cost effective– More preps for the money.

æ Eco-Friendly- Minimum number of steps, thus minimum

number of plastic ware required.

Ordering Information

Cat. # Product Pack Size

GD1004A GSure® Dogma Kit for Tissue 20 prep

GD1004 GSure® Dogma Kit for Tissue 50 prep

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

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Page 14: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Advantages

ü Complete Isolation from 200ul Blood Sample just in 1 Hr.

ü Fast and efficient purification of DNA, RNA and Protein

from 200 µl to 1ml Fresh Blood.

ü An easy to use silica membrane, spin-mini-column based

isolation kit for rapid, robust and reproducible isolation of

all three bio-molecules.

ü Required sample volume is less and easy workflow.

ü Delivers high quality integrated DNA, RNA and Protein in

every isolation.

ü Doesn't retain impurities from sample in the eluted DNA

and RNA.

ü Qualitatively and quantitatively better than any Phase

Separation Method.

Fig. 1:

DNA RNA and Protein were isolated from 1 ml of blood of 4 different

individuals.10% of the isolated biomolecules were electrophoresed on

gel. DNA and RNA were run on 1% Agarose TAE gel.

Fig. 2:

Total RNA was isolated from 1ml blood from individuals. RNA was

eluted in 50ml water and 1/10th fraction is loaded on 1% agarose gel.

Features

æ Efficient- DNA, RNA and Protein from same sample.

æ Fast– One hour required to isolate 3 bio-molecules

æ Convenient- Same optimized protocol for different

sources of sample.

æ High yield- Recovers 5µg DNA, 2µg RNA and 50µg

Protein from 200µl Fresh Blood.

æ User Friendly– Less sample volume and easy workflow

æ Reproducible- Delivers almost equal amount of yield on

every isolation

æ Easy – spin column format.

æ Cost effective– More preps for the money

æ Eco-Friendly- Minimum number of steps, thus minimum

number of plasticware required.

Fig. 3:

DNA was Isolated from 100pJ of whole blood, serum and WBC. 1/10th

volume was electrophored on 1% agarose gel..

Ordering Information

GSure® Dogma Kit for Blood

Cat # Product Pack Size

GD1005A GSure®Dogma Kit for Blood 20 prep

GD1005 GSure®Dogma Kit for Blood 50 prep

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

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Description

GCC Biotech offers One Step RT-PCR Kit for sensitive and end

point detection of RNA templates. One Step RT-PCR Kit

includes dNTPS, one step 10 X reaction buffer, enzyme mix

with reverse transcriptase and proof reading hot start DNA

polymerase and ribonuclease inhibitor.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 6 months when stored properly. Repeatative freeze-thaw

cycles should be minimized.

Fig. 1:

PCR amplification using 1 Step RT-PCR Kit from in vitro transcript RNA.

Lane M: molecular size marker -1kb DNA Ladder. Lanes A1 & A2 (1Kb),

B1 &B2 (1.5Kb), C1 & C2 (2Kb): PCR amplification reactions, using 10 x

One step RT Buffer with 0.2 mM dNTPs and 1U enzyme mix in 20 ml

reaction volume. 5 ml loaded for analysis in 1% agarose gel.

Features

æ cDNA synthesis and PCR amplification steps are

performed in a single reaction using gene specific primers

æ It is fast and easy one tube set up

æ Results in high-yield amplification with minimal

optimization

æ It can be used for any type of RNA template.

æ It is recommended in detection or quantification of

several mRNA from, a single step.

Kit Components

One Step RT-PCR Kit

G9 Taq DNA polymerase

Ÿ An ideal tool for standard PCR of templates of 6kb and shorter

Ÿ High throughput PCR from complex genomic, viral, and plasmid

Ÿ templates compatible for TA cloning and RT-PCR

Kit Component Taq (2.5 unit//ul); 10XTaq Buffer; 25 mM, MgCI2;

Control DNA template; Control Primer

Kit Size : 500U; 2X 500U; 10X500U

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Component G7113 G7113A

Enzyme Mix 1

Enzyme Mix 2

10X One-Step Reaction buffer

100mM MgCl 2

dNTP mix, 10 mM

Nuclease-free H O 2

Control RNA Template ( 300 ng,3Rxn)

Control Forward primer (10 mM)

Control Reverse primer (10 mM)

50 µl

50 µl

100 µl

25 µl

50 µl

1.5 ml

3 µl

3 µl

3 µl

100 µl

100 µl

200 µl

50 µl

100 µl

1.5 ml

3 µl

3 µl

3 µl

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Description

GCC Biotech offers Dual Step RT-PCR Kit for fast cDNA synthesis

enabling two-step RT-PCR for gene expression analysis. In the

first step, M-MulV Reverse Transcriptase (RT) is used with

random primer, oligodT primer or gene specific primer annealed

to an RNA sample. In the second step, PCR amplification is

performed in a separate tube using gene specific primers. A

ready to useTaq 2X PCR Master Mix is provided for its

convenient and consistent amplification performance.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 6 months when stored properly. Repetitive freeze-thaw

cycles should be minimized.

Fig.1.

PCR amplification using Dual Step RT-PCR Kit from Diffrent RNA Sources

Lane M: molecular size marker - Perfect 1kb DNA Ladder. Lanes 1

L(3kb),RNA from plant RNA and L2 RNA from animal cell line. cDNA

prepared using M-MulV Revers Transcriptase, 10x RT buffer dNTPs and

1U enzyme mix in 20 ml reaction volume.

Features

æ High cDNA yields even from low-abundance transcripts

æ Multiple transcripts can be detected from asingle first

strand cDNA synthesis

æ Semi quantitative analysis of the mRNA level can be

achieved by agarose gel electrophoresis.

Kit Components

Dual Step RT-PCR Kit

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

LGquant�Pico �Green�dsDNA�Quantitation�Reagent

£ An ultra-sensitive fluorescent nucleic acid stain

£ 10,000 times more sensitive than UV absorbance based measurements

£ Many applications in molecular biological procedures like cDNA synthesis for library production, DNA fragment purification for subcloning, as well as diagnostic applications

Component G7114 G7114A

M-MLV Reverse Transcriptase

(M-MLVRT) (200u/µl)

10X M-MLV RT Buffer with MgCl2

10 mM dNTP mix

2X Hi G9 Taq PCR Master Mix

Sterile, DEPC water

0.1 ml

0.2 ml

0.1 ml

1.25 ml X 2

1.5 ml

0.2 ml

0.4 ml

0.2 ml

1.25 ml X 4

1.5 ml

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Description

G9 Taq DNA Polymerase is a thermostable recombinant DNA

polymerase, expressed and purified from E.coli. G9 polymerase

catalyzes the polymerization of nucleotides into duplex DNA in

5'- 3' direction in the presence of magnesium ions , has no

detectable 3' 5' exonuclease (proofreading) activity and →

possesses low 5' 3' exonuclease activity. G9 Taq DNA →

polymerase supplied with a special and improved buffer system

that increases thermostability (enzyme half-life) and

processivity of Taq Polymerase by stabilizing the enzyme during

PCR. G9 Taq DNA Polymerase buffer is a combination of

different additives, which protects enzymes under stress

conditions such as desiccation, heat, and changes in pH and salt

concentration. G9 Taq polymerase shows a robust amplification

of templates even with higher complexity.

Applications

l High throughput PCR from complex genomic, viral,

and plasmid templates

l TA Cloning

l RT-PCR.

Unit Definition

One unit of G9 Taq DNA Polymerase is the amount of enzyme

required to incorporate 10 nmoles of deoxyribonucleotide

into DNA in 30 min at 74°C material in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. Avoid repeated

freeze/thawing of reagents.

Kit Components

Features

æ High cDNA yields even from low-abundance transcripts

æ Multiple transcripts can be detected from a single first

strand cDNA synthesis

æ Semi quantitative analysis of the mRNA level can be

achieved by agarose gel electrophoresis

æ PCR for higher fragment upto 6 kb.

Fig. 1: PCR amplification using G9 Taq DNA Polymerase and

Other Brand’s Taq polymerase.

Lane M: molecular size marker - 1 kb DNA Ladder. A comparative 4

and 6 kb PCR amplification reactions, using respective 10 X reaction

Buffer with 0.2 mM dNTPs and 1.25 U respective DNA polymerase in

50 μl reaction volume. 5 μl loaded for analysis in 1% agarose gel.

G9 Taq DNA Polymerase

Component G7115B G7115 G7115A

G9 Taq DNA Polymerase (2.5 unit//l)

10X Taq Reaction Buffer

25 mM MgCl2

Control DNA template

Control Primer

500 Unit

(2 X 1.25 ml)

(2 X 1.25 ml)

10�ml

10�ml

2X500 Unit

(2 X 1.25 ml)

(2 X 1.25 ml)

10�ml

10�ml

10X500 Unit

(20 X 1.25 ml)

(20 X 1.25 ml)

10�ml

10�ml

M G9

Taq

Oth

er B

ran

d

G9

Taq

6 kb

4 kb

Oth

er B

ran

d

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Page 18: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Description

Hi-G9 Taq DNA Polymerase is a thermostable recombinant

DNA polymerase, expressed and purified from E.coli. Like G9

polymerase this polymerase catalyzes the polymerization of

nucleotides into duplex DNA in 5'- 3' direction in the presence of

magnesium ions , has no detectable 3' 5' exonuclease →

(proofreading) activity and possesses low 5' 3' exonuclease →

activity. Hi-G9 Taq DNA polymerase is G9 Taq DNA polymerase

supplied with a special and improved buffer system that

increases thermostability (enzyme half-life) and processivity of

Taq Polymerase by stabilizing the enzyme during PCR. Hi-G9

Taq DNA Polymerase buffer is a combination of different

additives, which protects enzymes under stress conditions such

as desiccation, heat, and changes in pH and salt concentration.

Hi-G9 Taq polymerase shows a robust amplification of

templates even with higher complexity.

Applications

l High throughput PCR from complex genomic, viral,

and plasmid templates

l TA cloning

l PCR for high GC content amplicons.

l RT-PCR

Unit Definition

One unit of Hi-G9 Taq DNA Polymerase is the amount of

enzyme required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. Avoid repeated

freeze/thawing of reagents.

Kit Components

Features

æ Improved sensitivity, specificity High yields

æ Unique buffer formulation facilitates high throughput PCR

æ PCR for higher fragment upto 8 kb

æ Generates PCR products with 3'-dA overhangs.

Fig. 1:

PCR amplification using Hi-G9 Taq DNA 1st Lane: molecular size

marker - 1 kb DNA Ladder. 2nd and 3rd lane : 8 kb PCR amplification

reactions, using 10 X Hi-G9 taq reaction Buffer with 0.2 mM dNTPs

and 1.25 U Hi-G9 taq DNA polymerase in 50 μl reaction volume. 2 ml

loaded for analysis in 1% agarose gel.

Fig. 2.

Amplification of 200bp fragments from GC rich template (50%, 60%,

70% and 80% respectively) with Hi-G9 taq polymerase in presence of

GCfiX buffer.

Hi-G9 Taq DNA Polymerase

8kb

1 kb

la

dd

er

Percent of GC content50% 60% 70% 80%1

00 b

p

lad

der

Component GG03 GG01 GG02

Hi-G9 Taq DNA Polymerase (2.5 unit/λ)

10X Hi-G9 Taq Reaction Buffer

25 mM MgCl2

Control DNA template

Control Primer mix

500 Unit

(2 X 1.25 ml)

(2 X 1.25 ml)

10 µl

10 µl

(2X 500 Unit)

(4 X 1.25 ml)

(4 X 1.25 ml)

10 µl

10 µl

(10X 500 Unit)

(20 X 1.25 ml)

(20 X 1.25 ml)

10 µl

10 µl

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

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Page 19: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Description

Prime Taq DNA Polymerase is an optimized combination of G9

Taq DNA polymerase and high fidelity DNA polymerases from

Pyrococcus species for use in routine and difficult PCR

experiments. The 3´ 5´ exonuclease activity of the high fidelity →

DNA Polymerase increases the fidelity and robustness in

amplification by Taq DNA Polymerase, even from very low copy

number of template. The 10 X Reaction Buffer has also been

formulated for robust yield of desirable PCR products with

requirement of minimal optimization. Prime Taq DNA

polymerase is capable of amplifying amplicons up to 10kb.

Prime Taq DNA polymerase has improved efficiency of

polymerization irrespective of templates having high GC

content and contamination of exogenous PCR inhibitor. Prime

Taq DNA Polymerase is supplied with 10X Prime Taq Standard

Reaction Buffer to provide robust amplification.

Applications

l High throughput Long range PCR from complex

genomic, viral, and plasmid templates (up to 10 kb)

l Colony PCR

l GC-rich PCR

l AT-rich PCR

l Routine use PCR

l RT-PCR

Unit Definition

One unit of Prime Taq DNA Polymerase is the amount of

enzyme required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. Avoid repeated freeze

/thawing of reagents.

Kit Components

Features

æ A perfect blend of two different DNA polymerases for the

robust yield in PCR reaction of higher sized fragments.

æ Unique buffer formulation to facilitate improved

sensitivity, specificity, and yields for long range

æ Robust yield of long amplicons (up to 10 kb) as compared

to any Taq DNA polymerase.

Fig.1.

Amplification of differnt fegions from lamda DNA with Prime Taq DNA

polymerase, 10µl of PCR product were loded on 1% agarose gel.

Prime Taq DNA Polymerase

Component G4798 G4798A G4799

Prime Taq DNA Polymerase (2.5 unit/ml)

10X Prime Taq Reaction Buffer

25 mM MgCI2

Control DNA template

Control Primer mix

500 Unit

(2 X 1.25 ml)

(2 X 1.25 ml)

10 µl

10 µl

(2X500 Unit )

(2 X 1.25 ml)

(2 X 1.25 ml)

10 µl

10 µl

(10X500 Unit )

(20 X 1.25 ml)

(20 X 1.25 ml)

10 µl

10 µl

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Page 20: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Description

Hi-Prime Taq DNA Polymerase is an optimized blend of G9 Taq

DNA polymerase and high fidelity DNA polymerases from

Pyrococcus species in presence of specially formulated buffer

which supports PCR amplification from critical amplicons.

Presence of high fidelity DNA polymerases from Pyrococcus

species in optimized ratio and the enhancer buffer system make

Hi-Prime Taq DNA polymerase an excellent choice for PCR

amplification from natural isolates. The 3´ 5´ exonuclease →

activity of the high fidelity DNA Polymerase increases the fidelity

and robustness in amplification by Taq DNA Polymerase, even

from very low copy number of template.

Hi-Prime Taq DNA polymerase comes with a specially

formulated buffer, which allows amplification up to 12kb or

more.

Applications

l PCR from natural isolates.

l PCR for diagnostic samples.

l PCR for forensic samples.

l PCR for high GC content amplicons.

l PCR for soil DNA

Unit Definition

One unit of Hi Prime Taq DNA Polymerase is the amount of

enzyme required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. Avoid repeated freeze

/thawing of reagents.

Kit Components

Features

æ A perfect blend of two different DNA polymerases

æ for the robust yield in PCR reaction of higher sized

fragments

æ Unique buffer formulation to facilitate improved

æ sensitivity, specificity, and yields for long range

æ PCR ( ≥ 12 Kb)

Fig.1.

PCR amplification using Hi-Prime Taq DNA 1st Lane: molecular size

marker - 1 kb DNA Ladder. 2nd and 3rd lane: 12 kb PCR amplification

reactions, using 10X Prime Taq reaction Buffer with 0.2 mM dNTPs

and 1.25 U respective DNA polymerase in 50 μl reaction volume. 5 ml

loaded for analysis.in 1% agarose gel.

Hi-Prime Taq DNA Polymerase

Component G7116 G7116A G7116B

Hi-Prime Taq DNA Polymerase (2.5 unit/λ)

10X Hi-Prime Taq Reaction Buffer

25 mM MgCl2

Control DNA template

Control Primer mix

500 Unit

(2 X 1.25 ml)

(2 X 1.25 ml)

10 µl

10 µl

2X 500 Unit

(4 X 1.25 ml)

(4 X 1.25 ml)

10 µl

10 µl

10X 500 Unit

(20 X 1.25 ml)

(20 X 1.25 ml)

10 µl

10 µl

12 kb

1 k

b

lad

der

Heat Inactivation- No

3' to 5' Exonuclease activity- Yes

5' to 3' Exonuclease activity- Yes

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

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Description

G9 Taq DNA Polymerase is a thermostable DNA polymerase

having polymerase activity in 5'- 3' direction and and a 5´ flap

endonuclease activity.This recombinant protein was expressed

and purified from E.coli. The G9 2X Master Mix is an an

optimized ready-to-use solution containing Taq DNA

Polymerase, dNTPs, MgCl2 , KCI and stabilizers, requiring only

the addition of primers and DNA template for desired

amplification.

Applications

l PCR from wide range of natural isolates.

l Long PCR

l TA cloning

l Primer Extension

Unit Definition

One unit of PrimeTaq DNA Polymerase is the amount of

enzyme mix required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly.Avoid repeated

freeze/thawing of reagents

Kit Components

Features

æ Designed for easy and regular use

æ Half-life is more than 40 min at 95°C

æ Generates PCR products with 3'-dA overhangs

æ It can amplify up to 6 kb from lambda DNA.

Fig.1.

PCR amplification with 2X G9 Taq PCR Master Mix from lambda

genomic DNA 1st Lane: molecular size marker - 1 kb DNA Ladder. 2nd

and 3rd lane : 6 kb PCR amplification reactions, using 2X G9 Taq PCR

Master Mix in 50 ml final reaction volume. 5 ml loaded for analysis in

1% agarose gel.

2X G9 Taq PCR Master Mix

1 k

b la

dder

6 kb

With 2X Hi

G9 Taq PCR

Master Mix

Note:

ü 2X G9 Taq PCR Master Mix are recommended to use at a 1X concentration, adding DNA template and

primers in a total reaction volume of 25 or 50 μl.

ü 2X G9 Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.

ü In some complicated PCR, further MgCl need to be incorporated in final reaction mixture.2

Component G7117 G7117A G7117B

2x G9 Taq PCR Master Mix

Control DNA

TemplateControl Primer mix

100 reactions

10 µl

10 µl

250 reactions

10 µl

10 µl

1000 reactions

10 µl

10 µl

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Page 22: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

Description

Hi-G9 Taq PCR Master Mix is a thermostable DNA polymerase

having polymerase activity in 5'- 3' direction and and a 5´ flap

endonuclease activity. This recombinant protein was expressed

and purified from E.coli. 2x Hi G9 Taq PCR Master Mix is a robust

Taq master mix supplied with a buffer which is an optimized,

ready-to-use DNA Polymerases ideally suited to PCR

applications from complex genomic template bacterial colonies

and cDNA products. This convenient quick-load master mix

formulation contains dNTPs and different additive buffer

components to enhance processivity of G9 Taq Polymerase.

Applications

l Colony PCR

l Long PCR

l TA cloning

l Primer Extension

Unit Definition

One unit of Hi-G9 Taq DNA Polymerase is the amount of

enzyme required to incorporate 10 nmoles of deoxyribo

nucleotide into DNA in 30 min at 74°C in its buffer optimized

condition.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. 2X HiG9 Taq PCR Master

Mix is stable for 25-30 freeze-thaw cycles when stored at -

20°C. Avoid repeated freeze/thawing of reagents

Kit Components

Features

æ Easy to use for long range PCR

æ For sensitive high throughput PCR

æ Tolerates a wide range of genomic DNA templates

æ Generates PCR products with 3'-dA overhangs

æ It can amplify up to 8 kb from lambda DNA.

Fig. 1:

PCR amplification with 2X Hi G9 Taq PCR Master Mix from lambda

genomic DNA .1st Lane: molecular size marker - 1 kb DNA Ladder.

2nd and 3rd lane : 8 kb PCR amplification reactions, using 2X Hi G9

Taq PCR Master Mix in 50 ml final reaction volume.5 ml loaded for

analysis in 1% agarose gel.

2X Hi-G9 Taq PCR Master Mix

Note:

ü 2X Hi-G9 Taq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and

primers in a total reaction volume of 25 or 50 μl.

ü 2X Hi-G9 Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.

ü In some complicated PCR, further MgCl2 need to be incorporated in final reaction mixture.

1 kb

lad

der

8 kb

With 2X Hi

G9 Taq PCR

Master Mix

Component G4804 G4804A G4804B

2X Hi G9 Taq PCR Master Mix

Control DNA template

Control Primer mix

100 rxn

10 µl

10 µl

250 rxn

10 µl

10 µl

1000 rxn

10 µl

10 µl

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Note:

ü 2X Prime Taq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and

primers in a total reaction volume of 25 or 50 μl.

ü 2X Prime Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.

ü In some complicated PCR, further MgCl2 need to be incorporated in final reaction mixture.

Description

2X Prime Taq Master Mix with Standard Buffer is an optimized

blend of Taq and Hi fidelity DNA Polymerases ideally suited to

routine PCR applications from a variety of templates including

pure DNA solutions, bacterial colonies, and cDNA products. 2X

Prime Taq Master Mix is the best choice for PCR amplification

using natural isolates as template DNA. This Hi fidelity DNA

Polymerase increases the processivity and robust amplification

of Taq DNA Polymerase. The convenient master mix formulation

contains dNTPs, MgCl . KCl buffer components and stabilizers, 2

requiring only the addition of primers and DNA template for

robust amplification.

Applications

l PCR from wide range of natural isolates.

l Long PCR

l TA cloning

l Primer Extension

Unit Definition

One unit of PrimeTaq DNA Polymerase is the amount of

enzyme mix required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly. 2X HiG9 Taq PCR Master

Mix is stable for 25-30 freeze-thaw cycles when stored at -

20°C.Avoid repeated freeze/thawing of reagents

Kit Components

Features

æ Easy to use for long range PCR (upto 10 kb)

æ For sensitive high throughput PCR

æ Tolerates a wide range of genomic DNA templates

Fig.1.

PCR amplification with 2X Prime Taq PCR Master Mix from lambda

genomic DNA . 1st Lane: molecular size marker - 1 kb DNA Ladder.

2nd and 3rd lane : 10 kb PCR amplification reactions, using 2X Prime

Taq PCR Master Mix in 50 ml final reaction volume. 5 ml loaded for

analysis in 1% agarose gel.

2X Prime Taq PCR Master Mix

1 kb

lad

der

10 kb

With 2X

Prime Taq PCR

Master Mix

Component G7118 G7118A G7118B

2X Prime Taq PCR Master Mix

Control DNA Template

Control Primer mix

100 rxn

10 µl

10 µl

250 rxn

10 µl

10 µl

1000 rxn

10 µl

10 µl

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Description

2X Hi-Prime Taq Master Mix with PCR enhancer cocktail is an

optimized combination of Taq and Hi fidelity DNA Polymerases

present in a buffer that is compatible to amplify from different

template source, irrespective of their GC content. This 2X Master

Mix particularly resistant to some PCR inhibitors up to a certain

percentage. The mix is suitable for direct PCR from unprocessed

samples including bacterial culture, bacterial colonies. 2X Hi

Prime Taq Master Mix can perform consistently well on a broad

range of templates (including both GC and AT rich). The

convenient master mix formulation contains dNTPs, MgCl2,

buffer components and stabilizers, requiring only the addition

of primers and DNA template for robust amplification.

Applications

l Genotyping.

l Multiplex PCR

l Library construction

l PCR from bacterial culture and urine

Unit Definition

One unit of Hi-PrimeTaq DNA Polymerase is the amount of

enzyme mix required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed

stable for 2 years when stored properly. 2X Hi-Prime

Taq PCR Master Mix is stable for 25-30 freeze-thaw

cycles when stored at -20°C. Avoid repeated

freeze/thawing of reagents.

Kit Components

Features

æ Easy to use for long range PCR (upto 12 kb)

æ For sensitive high throughput PCR

æ Tolerates a wide range of genomic DNA templates

Fig.1.

PCR amplification with 2X Hi Prime Taq PCR Master Mix from lambda

genomic DNA 1st Lane: molecular size marker - 1 kb DNA Ladder.

2nd and 3rd lane: 12 kb PCR amplification reactions, using 2X Hi-

Prime Taq PCR Master Mix in 50 ul final reaction volume. 5 ul loaded

for analysis in 1% agarose gel.

2X Hi-Prime Taq PCR Master Mix

Note:

ü 2X Hi-PrimeTaq PCR Master Mix is recommended to use at a 1X concentration, adding DNA template and

primers in a total reaction volume of 25 or 50 μl.

ü 2X Hi-Prime Taq PCR Master Mix is stable for 25-30 freeze-thaw cycles when stored at -20 ° C.

Component G7119 G7119A G7119B

2X Hi PrimeTaq PCR Master Mix

Control DNA template

Control Primer mix

100 rxn

10 µl

10 µl

250 rxn

10 µl

10 µl

1000 rxn

10 µl

10 µl

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Description

Gpfu Polymerase is a thermostable DNA polymerase isolated

from hyperthermophilic Pyrococcus furiosus. The protein is

isolated from a recombinant E.coli strain containing the gene

encoding Pfu DNA polymerase. It catalyzes the DNA-dependent

polymerization of nucleotides into duplex DNA in the 5' 3' →

direction and exhibits 3' 5' exonuclease (proof reading) →

activity. Pfu DNA polymerase is the ideal choice for a variety of

techniques requiring high-fidelity DNA synthesis by PCR

reaction. It can apply to cloning, gene expression, site-directed

mutagenesis and etc.

Applications

l High Fidelity PCR can amplify up to 4 kb

l Blunt-end PCR Cloning or mutagenesis requested

high fidelity

l Site directed mutagenesis

Unit Definition

One unit is defined as the amount of enzyme required to

catalyze the incorporation of 10nmoles of dNTP into acid

insoluble material in 30 min at 74°C.

Storage

-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed

stable for 2 years when stored properly. Avoid repeated

freeze / thawing of reagents.

Kit Components

Features

æ Easy to use for long range PCRFor sensitive high

throughput PCR

æ Tolerates a wide range of genomic DNA templates

æ Generates PCR products with 3'-dA overhangs.

Fig.1.

PCR amplification using GPfu DNA Polymerase. Lane M: molecular

size marker - 1 kb DNA Ladder. Lanes 2 to 6 kb: PCR amplification

reactions, using 10 X GPfu Buffer with 0.2 mM dNTPs and 1 U GPfu

DNA Polymerase in 50 μl reaction volume. 5μl loaded for analysis in

1% agarose gel.

Gpfu Polymerase

M 2 4 kb

Component G7122 G7122A

Gpfu Polymerase (1 Unit/λ)

10X GPfu Reaction Buffer

45 mM MgCl2

Control DNA template

Control Primer mix

100 Unit

(2 X 0.625 ml)

(2 X 0.625 ml)

10 µl

10 µl

2X100 Unit

(4 X 0.625 ml)

(4X 0.625 ml)

10 µl

10 µl

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Description

DeltaQ polymerase is a truncated version of Taq DNA

Polymerase, lacking the first 278 amino acids. To enhance its

DNA binding affinity a dsDNA binding domain protein has been

fused with this truncated polymerase. Normally DeltaQ

polymerase is able to amplify upto 2 kb from lambda DNA. Its

enhanced binding property endow It to amplify from very low

amount template (~ fmole level). DeltaQ polymerase also

contains mutations in its polymerase domain that make it

resistant to inhibitors present in body fluid. This resistant nature

to pcr inhibitors, makes DeltaQ polymerase a suitable choice for

PCR from whole blood , serum, urine. DeltaQ polymerase

tolerates up to 15% whole blood and 50-60% blood serum in a

50 ml reaction which make it unique in its character from rest of

the available polymerases. Apart from this special characteristics

DeltaQ polymerase can amplify from high GC rich template.

Applications

l PCR for diagonistic samples

l PCR for forensic sample

l PCR for GC rich amplicon

l RT-PCR

l qPCR

Unit Definition

One unit of Delta Q Taq DNA Polymerase is the amount of

enzyme required to incorporate 10 nmoles of

deoxyribonucleotide into DNA in 30 min at 74°C.

Storage

-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed

stable for 2 years when stored properly. Avoid repeated

freeze / thawing of reagents.

Kit Components

Features

æ Easy to use for PCR upto 2 kb

æ Designed to be compatible with existing assay systems

æ Amplify from very low amount of template

æ Resistant to PCR Inhibitors, can amplify in

æ presence of blood, serum and urine

æ Generates PCR products with 3'-dA overhangs

Characteristics of Delta Q: A. DeltaQ can amplify upto 2 kb on lambda DNA template.

B. DeltaQ can amplify on minimum amount of template (from femtogram amount). 1 kb

PCR on lambda DNA from indicated amount of template given template.

C. Extension speed of taq polymerase. DeltaQ can amplify 2 kb size fragment at a minimum

of 30 sec/ kb speed during extension.

D. Amplification on GC rich template: DeltaQ can amplify on strong template like high GC

content in presence of special buffer (up to 70%).

E. PCR amplification in presence of whole blood:

DeltaQ can tolerate up to 15% whole blood in PCR mix (7.5 µlit in 50 µlit reaction),

allowing a wide range of blood usage during direct PCR from blood (as template).

F. A comparative of amplification efficiency of DeltaQ, G9 taq and Vent polymerase: 1 kb

lambda based PCR was done in presence of 10%, 12% whole blood with the three

polymerases in their respective buffers.

Delta Q Polymerase

100 60 20 2

1 k

b la

dder

5% 8% 10% 12% 15%

1 k

b l

ad

de

r

1 kb

HighDeltataQ G9 taq polymerase

WHOLEBLOOD 10% 10% 10% 12%12%12%

Vent

GC Rich Template

70%65%

200bp

1 k

b lad

der

1 k

b lad

der

5 s

ec/k

b

10 s

ec/k

b

15 s

ec/k

b

30 s

ec/k

b

2 kb

A

C D

E

F

WHOLEBLOOD

Component G7122 G7122A

DeltaQ Polymerase (1unit/λ)

10X DeltaQ Reaction Buffer

30 mM MgCl2

Control DNA template

Control Primer mix

250 Unit

1 X 1.25 ml

1 X 1.25 ml

10 µl

10 µl

4 X 250 Unit

4 X 1.25 ml

4 X 1.25 ml

10 µl

10 µl

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Description

T4 DNA Ligase catalyzes the joining by the formation of

phosphodiester bonds between 3'-OH termini and 5'-P termini

of sticky or blunt ended DNA strands. The molecular weight of

T4 Ligase is ~62,000 Da. For ligation, the optimal reaction pH is 2+7.6, and T4 ligase requires Mg and ATP as cofactors.

Applications

l Cloning of restriction enzyme generated DNA

fragments.

l Cloning of PCR products.

l Joining of double-stranded oligonucleotide linkers or

adaptors to DNA.

Unit Definition

One unit is defined as the amount of enzyme required to give

50% ligation of HindIII fragments of λ DNA (5´ DNA termini

concentration of 0.12 µM, 300- µg/ml) in a total reaction

volume of 20 ml in 30 minutes at 16°C in 1X T4 DNA Ligase

Reaction Buffer.

Storage

-20 ° C to -10 ° C in a non-frost-free freezer. Guaranteed

stable for 2 years when stored properly. Avoid repeated

freeze / thawing of reagents.

Kit Components

Featurel Nick repair in duplex DNA.

l Self-circularization of linear DNA.

l Fully Ligated with in 30 minit at Room Temperature

Source

æ E.coli cells with a cloned gene 30 from bacteriophage T4.

Inhibition and Inactivation

æ T4 DNA Ligase is strongly inhibited by NaCl or KCl at

concentrations higher than 200 mM.

æ Inactivated by heating at 65°C for 10 min or at 70°C for 5

min.

T4 DNA Ligase

Component GTDL01 GTDL02 GTDL03

T4 DNA Ligase(5U/ul)

10X T4 DNA Ligase

Buffer

100 Unit

150�ml

250 Unit

375�ml

1000 Unit

1500�ml

l D

NA

Dig

est

ed

Usi

ng

Hin

dII

I

Lig

ate

d w

ith

T4

DN

A L

igase

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Description

Codon optimized Taq DNA Polymerase gene of Thermus

aquaticus was cloned and purified from E. coli host. The enzyme

consists of a single polypeptide with a molecular weight of

approximately 94 kDa. Ready Load PCR Master Mix is a ready-

to-load master mix. It is a convenient way of amplifying DNA

fragments without the need to thaw individual components,

reducing the risk of contamination and pipetting errors.

Presence of a tracking dye helps to load and track migration of

DNA on agarose gel. The G9 Taq DNA Polymerase, dNTPs,

reaction buffer and magnesium chloride are all present in the

mix. Tracking dye is present in such an amount that its migration

should be visible but should not mask DNA to observe. Presence

of precipitant and tracking dye eliminates any chances of

pipetting error prior gel load thus making this 2X G9 mix as an

appropriate choice for semiquantitative PCR analysis.

Applications

l Ready Load PCR Master Mix is a ready-to-load master

mix

l Tracking dye is present in such an amount that its

migration should be visible but should not mask DNA

to observe

Unit Definition

One unit incorporates 10nmol of deoxy-ribonucleotide into

acid-insoluble product in 30 minutes at 74°C. Unit assay

conditions: 25 mM TAPS (pH 9.3), 50 mM KCl, 2 mM MgCl2, 1

mM DTT, 0.2 mM dATP, dCTP, dGTP, dTTP utilizing

M13mp18DNA as template.

Storage

-20°C to -10°C in a non-frost-free freezer. Guaranteed stable

for 2 years when stored properly.Avoid repeated

freeze/thawing of reagents

Kit Components

Features

æ Reducing the risk of contamination and pipetting

errors

æ Designed for easy and regular use

æ Half-life is more than 40 min at 95°C

æ Generates PCR products with 3'-dA overhangs

æ It can amplify up to 6 kb from lambda DNA.

Fig.1.

PCR amplification with 2X G9 Taq Readyload PCR Master Mix from

lambda DNA 1st 2nd and 3rd lane : 6 kb PCR amplification reactions,

using 2X G9 Taq Readyload PCR Master Mix in 50 ml final reaction

volume. 5 ml loaded for analysis in 1% agarose gel. 4th Lane: molecular

size marker - 1 kb DNA Ladder.

2X G9 Taq Readyload PCR Master Mix

Component G7124 G7124A

2X PCR Master Mix

Control DNA template

Control primer Mix

(1000 ml X 2)

10�ml

10�ml

(1000 ml X 20)

10�ml

10�ml

1 kb

lad

der

6 kb Amplification of

l DNA using Ready to

load Master Mix

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PolymeraseStaini

ng Reagents

5′–>3′

Exonuclease

3′–>5′

Exonucleaselimit

Thermal

StabilityRequire

Extension

Rate

Extension

from NickApplications

G9 Taq

polymerase &

Master Mix

+ - + 1 kb/ min + Routine PCR up to 6 kb

Hi-G9 Taq

polymerase &

Master Mix

+ - + 1 kb/ min +

Routine PCR upto 8 kb, with

high productivity, template

with High GC content.

Prime Taq

polymerase &

Master Mix

+ - ++ 1 kb/ min + Long PCR up to 10 kb.

Hi prime Taq

polymerase &

Master Mix

+-

++ 1 kb/ min +

Long PCR more than 12 kb

(with enhanced

productivity)

Gpfu DNA

polymerase- + + 0.5 kb/ min -

High-fidelity PCR, (such as

gene cloning, gene

expression or mutation

analysis), upto 4 Kb

DeltaQ

polymerase- - - 2 kb/ min +

Low template requirement,

Direct PCR from body fluid,

RT PCR

upto 2 Kb.

Molecular Biology

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Plant Leaf / Blood / Tissue

Prescribed

Sample

amount

Incubate in fastract Buffer>O95 C for 15 min

Use as Template for PCR reaction

Collect

Fig. 1.: Streamline Protocol for FasTract Direct PCR Kit

DNA isolation is a time consuming, laborious procedure

specially when working with a huge number of samples.

Moreover sometimes purified DNA contains carry-over

inhibitors from crude extraction methods. FasTract Direct PCR

Kit provides ease for direct PCR from a wide variety of samples

without isolation of total genomic DNA. FasTract Direct PCR Kits

are designed to perform PCR directly from crude samples like

plant leaves, animal tissues, blood, saliva, cultured cells and even

samples like scalp and body hair without prior DNA isolation

and purification. Chaotropic salts and detergents present in

FasTract Lysis Buffer ensure better and efficient lysis even with

tough samples like animal tissues and hair. FasTract PCR Mix

contains a genetically modified Hi Fidelity DNA polymerase

which can amplify even in presence of a large number of PCR

inhibitors. The kit ensures high yield in PCR amplification and is

time saving as DNA isolation from samples can be avoided prior

to PCR. The kit is recommended for end-point PCR. Samples are

needed to collect as the prescribed amount, incubated at 950C

in presence of FasTract Lysis buffer for 15 min only. This lysate is

to be used for PCR reaction as template. The lysate is only

compatible with the FasTract PCR master mix.

When working with plant samples, like plant leaves storage of

samples often becomes a matter of concern. FasTract Lysis

Buffer that comes along with FasTract Plant Direct PCR Kit is an

ideal storage buffer for leaf samples that will be further used for

direct amplification. Leaf samples can be stored in FasTract lysis 0buffer at room temperature for 5 days, at 4 C upto 4 weeks and

0indefinite time at -20 C .

Body fluids like saliva, clotted blood, buccal and pap swabs and

even tough samples like skin and hair are suitable samples for

FasTract Direct PCR Kit. Thus the kit finds huge application in

diagnosis of clinical samples and even for forensic detection.

Introduction

Fastract Direct PCR Kit

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Introduction

Plant DNA isolation is a time consuming, laborious procedure

specially when working with a huge number of samples. FasTract

Plant Direct PCR Kit is designed to perform PCR directly from

plant leaves without prior DNA isolation and purification. Fresh

plants, plant material stored at 4°C or frozen are all suitable

templates for this kit. Plant leaves could also be collected

directly in the FasTract plant Lysis Buffer and stored at room 0temperature for at least 48hr, at 4oC for 5 days and at -20 C for

at least one months. Caotrophs and detergents present in

FasTract Lysis Buffer ensure better and efficient lysis even with

tough samples like palm and coconut. FasTract plant PCR Mix

contains a genetically modified Hi Fidelity DNA polymerase

which can amplify even in presence of PCR inhibitors in plant

lysate. The kit ensures high yield in PCR amplification and is time

saving as DNA isolation from samples can be avoided prior to

PCR. The control primers provided can amplify a highly

conserved region from plant DNA. Purified plant gDNA is also

provided as a positive control for the PCR reactions. The kit is

recommended for end-point PCR.

Guidelines of Sample Preparation

l Single hole paper puncher must be used to obtain

small and uniform leaf discs (2mm diameter)

l It is very important to clean the cutting edge every

time before sampling to prevent cross-contamination

between samples

l 2 % sodium hypochlorite solution or 70% Ethanol

should be used for cleaning.

l Punch out a disc from the plant leaf using the

sampling tool and place disc directly into the PCR

tubes.

l Add 50ml Fas ract Lysis Buffer into each tubes,

l vortex well and incubate them at 950C for 15mins.

l Use 2. 5ml of this lysate as template for the PCR

reaction

l The FasTract Lysis buffer can be used for collection

and storage of leaf disc samples.

Guidelines for Control reaction

We recommend setting up a control PCR reactions with both the

purified DNA (provided) as well as the FasTract leaf disc lysate

used in the actual experiment to ensure that the PCR conditions

are optimal. If the positive control with purified DNA fails, the

PCR conditions should be optimized before continuing further.

It is recommended to add a no-template control to all PCR

assays.

Fig.1.

Different plant leaves after incubation with FasTract lysis

buffer.

Fig.2.

Endpoint PCR of 35 cycles was done using plant specific RBCL primer pair

Oon various plant leaves Leaf discs were incubated at 95 C for 15 mins 50

ml of Fastract modified buffer. 2.5 ml of this is used as template.

Gra

m

Orc

hid

Maiz

e

Pa

lm

Man

go

Ch

illi

Co

locasi

a

Co

co

nu

t

Pa

paya

Un

kn

ow

+ve c

on

100

bp

L

Fastract Direct PCR Kit for Plant

Key Components Amount

Fastract Lysis Buffer

Fastract 2X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP(10mM))

Control Primer Mix (10mM each)

50 ml

22 ml

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Guidelines For PCR setup

l Take a single leaf disc in a PCR tube and add 50µl of

FasTract Lysis Buffer in it.

l For samples collected directly in FasTract Lysis buffer,

next step is not required for soft plant leaves. S t i l l ,

heating the samples will ensure better lysis.

l Vortex the sample vigorously and incubate the FasTract oLysis Buffer added sample at 95 C for 15 min in a PCR

machine lead heating condition.

l Add 22ml 1X Fastract PCR mix in a Flat Capped PCR

tube.

l Add 0.25 ml of each Primers (from 10mM stock) to the

master mix.

l Use 2. 5ml of FasTract lysate as template for the PCR

reaction.

l Set up PCR as below.

Troubleshooting

l For tough and dry leaf samples increase the oincubation time at 95 C

l If PCR fails due to high amount of PCR inhibitors in

the leaf disc lysate, then the amount of lysis buffer can

be increased to 75-100ml

l If PCR fails even after increasing the volume of lysis

buffer, incubate half of the leaf disc in lysis buffer

instead of the entire disc.

Storage

FasTract Direct PCR Kit® is shipped on gel ice. Upon arrival,

store the components at +4 ° C. Do not freeze.

Ordering Information

Important Notes

æ Carefully mix and spin down all tubes before opening to

ensure homogeneity and improve recovery. The PCR setup

can be performed at room temperature.

æ Always add the plant sample last to the reaction.

æ It is recommended to eject the leaf disc into the empty tube

and add the lysis buffer to ensure that the entire disc is

dipped into buffer. Make sure that you see the sample disc in

the solution.

æ We recommend using fresh plant material for best results,

oeven though plant material stored at +4 ° C or -20 C can also

be used.

æ For extension, use 1min for 1Kb amplicon size.

æ The FasTract Lysate must be added such that it is 10% of the

total reaction volume i.e if the total reaction volume is 25ml

then 2.5ml of leaf disc lysate must be added to the reaction.

Cat. # Product Pack Size

G45311AFasTract Direct PCR

Kit® for Plant100 rxn

G45311BFasTract Direct PCR

Kit® for Plant500 rxn

95 ° C 95 ° C72 ° C 72 ° C

40x

10 s

t min 5 min

hold

5 min 10 sTm

t min= extension time: 1 min /kb

PCR Cycle

4 ° C

Fastract Direct PCR Kit for Plant

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Fastract Direct PCR Kit for Blood

IntroductionFasTract Blood Direct PCR Kit is designed to perform PCR

directly from blood, and saliva without prior DNA purification.

Freshly collected blood, blood stored with anticoagulants,

clotted blood and even blood spots on paper discs, all are

suitable samples for this FasTract Blood Direct PCR kit. The kit

employs a specially engineered high fidelity DNA Polymerase

enzyme that exhibits high resistance to many PCR inhibitors

found in blood and other bio-fluids. The kit ensures high yield

and is time saving as DNA isolation from samples can be avoided

prior to PCR. The kit is recommended for end-point PCR.

Fig.1.

CR reaction on blood, collected from different animals, using

species specific GAPDH primers. 20ml of blood samples were

lysed using 50ml of FasTract Blood Lysis Buffer and PCR reaction

was set up using FasTract 1X mastermix for blood

Guidelines of Sample Preparationl Blood stored with anticoagulants, clotted blood and

even blood spots on paper discs, all are suitable samples

for this Fastract Blood Direct PCR kit.

l When working with clotted blood or blood, 10-20 ml

blood is recommended

l In case of spotted blood on paper discs use a single hole

paper puncher to punch out a spotted blood disc. This

will be the sample.

l Collect the blood samples in a PCR tube , add 100 ml

Fastract Blood Lysis Buffer into each tubes, vortex well 0and incubate them at 95 C for 15 mins.

l After incubation centrifuge the PCR tubes at 15000 rpm

for 2 mins. Collect the clear supernatant in a separate

microfuge tube

l Use 1.5 ml of this clear lysate as template for the PCR

reaction.

Guidelines of PCR Setupl Add 47.5 ml 1X Fastract Blood Direct PCR mix in a Flat

Capped PCR tube.

l Add 0.5 ml of each Primers (from 10mM stock) to the master

mix.

l Use1. 5 ml of Fastract Blood lysate as template for the PCR

reaction.

l While setting up the PCR cycle keep the initial denaturation 0time to be 5 mins at 950C followed by denaturation at 95 C

for 10 -15s and annealing at the desired Tm. For extension,

use 1min for 1Kb amplicon size.

Troubleshootingl In case of no PCR reaction dilute the lysate In case of non-

specific amplification, increase the annealing temperature,

reduce the total number of cycles or design a new set of

primers.

Shipping and storagel Fastract Tissue Direct PCR Kit is shipped on gel ice. Upon

arrival, store the components at +4 ° C. Do not freeze.

Important Notesl Carefully mix and spin down all tubes before opening to

ensure homogeneity and improve recovery. The PCR setup

can be performed at room temperature.

l Always add the Blood lysate last to the reaction.

l When working with clotted blood vortex vigorously after

addition of lysis buffer

l Always ensure that the volume of lysate that is being added

to the PCR reaction is 3% of the total reaction volume. Do

not add more lysate to the reaction mix.

Key Components

FasTract Blood Buffer

FasTract 2X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP(10mM))

Control Primer Mix (10mM each)

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DescriptionFastract Direct PCR Kit™ for Blood is a rapid PCR mastermix that

eliminates the requirement of genomic DNA isolation prior PCR

reaction. This improved master mix is a ready load 2X Master

Mix, allowing researcher to load the PCR product directly in the

gel. Addition of loading dye is not required. FasTract direct PCR

kit comes with three different components.

1. RBC Lysis Buffer

2. Pellet dissolving buffer

3. 2X mastermix

This is recommended to lyse the RBC and harvest the WBC

(protocol provided with the kit) which to be resuspended and

lysed in Pellet dissolving buffer. This lysate would be used as

template for the PCR reaction. Fastract amplification after

removal of RBC is always recommended because it helps to have

efficient amplification for long amplicons and also for multiplex

amplification (Figure 1 and Figure 2). Direct lysis of whole blood

followed by amplification with Fastract PCR master mix shows

efficient PCR reaction for short amplicons (less than 500bp) but

the same for long amplicons (more than 1 kb) are compromised

(Figure 3 and 4 respectively). Amplification efficiency from blood

with nucleated RBC's are also checked and efficient

amplification were observed (Figure 5). The pellet dissolving

buffer has been formulated in such a way, lysate would be

compatible to amplify with any market leading Taq DNA

Polymerase (Figure 6).

Fig.1.

Amplification from direct blood sample Blood samples were

processed to remove RBCs and lysate were prepared from WBCs

only. 40 cycle endpoint PCR directly from lysate to amplify a

small amplicon from b-actin gene (<1kb) and a large amplicon

from G6PD gene (>1kb) with Fastract Blood Direct PCR Kit.

Fig.2.

Multiplex PCR using FasTract Direct PCR Kit 40 cycle multiplex

PCR directly from blood lysate to amplify GAPDH and H2B gene

specific region with Fastract Blood Direct PCR Kit.

Fig.3.

Equal amplification efficiency with or without vigorous mixing

Different volume of xeroPolymerase were reconstituted with or

without vortexing after addition of water, primers and

templates. Equal amplification found in both the cases.

Fastract Direct PCR Kit for Blood

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Fastract Direct PCR Kit for Blood

Fig. 4.

Amplification efficiency for short sized amplicon 40 cycle

endpoint PCR with Fastract Blood Direct PCR kit. PCR is done

directly from different volumes of human blood lysed in 30 ml

FasTract Blood Pellet Dissolving Buffer. Different volume of

blood lysate is used as template to amplify housekeeping gene

b-actin from human genome. WBC lysate is used as appositive

control for the reaction.

Fig.5.

FasTract Direct PCR amplification on blood with nucleated RBC

40 cycle endpoint PCR with Fastract Blood Direct PCR Kit from

species with nucleated RBC's like Fish and Chicken. 3ml of blood

is lysed in different volumes of Fastract Blood Lysis Buffer and

different volume of lysate is used as template for PCR to amplify

a housekeeping gene from the individual species. Genomic DNA

is used as a positive control for PCR amplification.

Fig.6.

Compatibility of Fastract Lysis Buffer with other Taq DNA

Polymerase 40 cycle endpoint PCR with human blood lysate to

amplify housekeeping gene b-actin from human genome. Blood

lysate is prepared with Fastract Blood Lysis buffer and the lysate

is used as a template for PCR with G9.

Ordering Information

Cat. # Product Pack Size

G45312A FasTract Direct PCR Kit® for Blood 100 rxn

G45312B FasTract Direct PCR Kit® for Blood 500 rxn

G45312 FasTract Direct PCR Kit® for Blood 1000 rxn

G45312QFasTract Blood lysis Buffer for

Blood (Without Mastermix)1000 rxn

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DescriptionFasTract Cultured cell Direct PCR Kit is designed to perform PCR

directly from cultured cell without prior DNA purification. Fresh

or frozen cells all are suitable templates for this kit. The kit

employs a specially engineered high fidelity DNA Polymerase

enzyme that exhibits high resistance to many PCR inhibitors

found in cell or tissue culture media. The kit ensures high yield

and is time saving as DNA isolation from samples can be avoided

prior to PCR.The kit is recommended for end-point PCR.

Fig.1.

PCR on cell lines using specific GAPDH Primers for CHO and b-

actin for the human cell lines. 0.2 million cells were lysed using

50ml of Fastract lysis buffer and PCR reaction was set up using

Fastract 1X Master Mix for Cultured cells

Guidelines of Sample Preparationl When working with cultured cell, use maximum 1 million or

as least as 10000 cells.

l The cells must be pellet down and must be washed with 1X

PBS 2-3 times.

l The cells must be suspended in 1X PBS solution

l Collect 25 ml of this cell suspension in a PCR tube, add 100ml

FasTract Cultured cell Lysis Buffer into each tubes, vortex owell and incubate them at 95 C for 15 mins.

l After incubation centrifuge the PCR tubes at 15000rpm for 2

mins. Collect the clear supernatant in a separate micro-fuge

tube

l Use 1.5 ml of this clear lysate as template for the PCR

reaction.

Troubleshootingl In case of no PCR reaction dilute the cell lysate 1:10 or

1:50 with FasTract cultured cell lysisbuffer and use 1.5 ml

of this dilution as template.

l In case of non-specific amplification, increase the

annealing temperature, reduce the total number of cycles

or design a new set of primers.

l

Shipping and storagel Fastract Tissue Direct PCR Kit is shipped on gel ice. Upon

arrival, store the components at +4°C. Do not freeze.

l

Important Notesü Carefully mix and spin down all tubes before opening to

ensure homogeneity and improve recovery. The PCR setup

can be performed at room temperature.

ü Always add the Blood lysate last to the reaction.

ü Always ensure that the volume of lysate that is being added

to the PCR reaction is 3% of the total reaction volume. Do

not add more lysate to the reaction mix

Important Notes

Key Components

FasTract Cell Lysis Buffer

FasTract Tissue 1X PCR Mix (FasTract DNA Polymerase, Reaction buffer, dNTP (10mM))

Control Primer Mix (10mM each)

Cat. # Product Pack Size

G45313AFasTract Direct PCR Kit® for

Cultured Cell100 rxn

G45313FasTract Direct PCR Kit® for

Cultured Cell500 rxn

Fastract Direct PCR Kit for Cultured Cell

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DescriptionFastract Tissue Direct PCR Kit is designed to perform PCR directly

from non-fixed animal derived tissue samples tissue samples

without prior DNA purification. Fresh tissues or tissue samples 0frozen at -20 C are all suitable templates for this kit. The kit

employs a specially engineered high fidelity DNA Polymerase

enzyme that exhibits high resistance to many PCR inhibitors

found in animal tissues. The kit ensures high yield and is time

saving as DNA isolation from samples can be avoided prior to

PCR. The kit includes a pair of universal control primers that is

compatible with a number of vertebrate species .The kit is

recommended for end-point PCR.

Fig.1.

PCR on tissue samples collected from different animals using

species specific GAPDH Primers.10mg tissue samples were lysed

using 50 ml of Fastract lysis uffer and PCR reaction was set up

using Fastract 1X mastermix for Tissue

Fig.2.

Fastract Lysis and PCR on different tissue of mice.10mg of tissue

samples were lysed in Fastract Lysis Buffer and PCR reaction

were done using FasTract 1X Master Mix.

Guidelines of Sample Preparationl When working with tissue samples, use of 2mg of tissue is

recommended for direct PCR

l Use a scalpel to cut out the tissue.

l The scalpel must be thoroughly cleaned with 2% Sodium

hypochlor ide or 70% Ethanol to prevent cross

contamination.

l Collect the tissue samples in a PCR tube, add 100 ml Fastract

Tissue Lysis Buffer into each tubes, vortex well and incubate

them at 950C for 15 mins.

l After incubation centrifuge the PCR tubes at 15000 rpm for 2

mins. Collect the clear supernatant in a separate microfuge

tube.

l Use 1.5 ml of this clear lysate as template for the PCR

reaction.

Guidelines for PCR Setupl Add 47.5ml 1X Fastract Tissueu cell Direct PCR mix in a Flat

Capped PCR tube

l Add 0.5ml of each Primers (from 10mM stock) to the master

mix

l Use1.5ml of Fastract cultured cell lysate as template for the

PCR reaction

l While setting up the PCR cycle keep the initial denaturation

time to be 5 mins at 950C followed by denaturation at 950C

for 10 -15s and annealing at the desired Tm. For extension,

use 1min for 1Kb amplicon size.

Troubleshootingl In case of no PCR reaction dilute the tissue lysate 1:10 or

1:50 with Fastract Tissue lysis buffer and use 1.5ml of this

dilution as template

l In case of non-specific amplification, increase the

annealing temperature, reduce the total number o f

cycles or design a new set of primers.

Important Notesü Carefully mix and spin down all tubes before opening to

ensure homogeneity and improve recovery. The PCR

setup can be performed at room temperature.

ü Always add the Tissue sample lysate last to the reaction.

ü It is recommended to add the tissue samples into the empty

tube and add the lysis buffer to ensure that the entire

sample is dipped into buffer. Make sure that you see the

sample in the solution.

ü Always ensure that the volume of lysate that is being added

to the PCR reaction is 3% of the total reaction volume. Do

not add more lysate to the reaction mix

ü Always ensure that the volume of lysate that is being added

to the PCR reaction is 3% of the total reaction volume. Do

not add more lysate to the reaction mix.

Fastract Direct PCR Kit for Tissue

Key Components

Fastract Tissue Lysis Buffer

Fastract Tissue 1X PCR Mix (FasTract DNA Polymerase, Reaction buffer, dNTP (10mM))

Control Primer Mix (10mM each)

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DescriptionFasTract Tissue Direct PCR Kit is designed to perform PCR

directly from hair blood, and saliva without prior DNA

purification. Freshly collected blood, blood stored with

anticoagulants, clotted blood and even blood spots on paper

discs, all are suitable samples for this FasTract Blood Direct PCR

kit. The kit employs a specially engineered high fidelity DNA

Polymerase enzyme that exhibits high resistance to many PCR

inhibitors found in blood and other biofluids. The kit ensures

high yield and is time saving as DNA isolation from samples can

be avoided prior to PCR. The kit is recommended for end-point

PCR.

Fig.1.

FasTract Lysis and PCR using different tissues of mice. 10 mg

of tissue samples were lysed in FasTract Lysis Buffer and PCR

reaction were done using FasTract 1X Master Mix

Guidelines of Sample Preparationl Collect the samples in a PCR tube, add 100 ul Fastract

Forensic Lysis Buffer into each tube, vortex well and incubate othem at 95 C for 15 mins.

l After incubation, centrifuge the PCR tubes at 15000 rpm for

2 mins. Collect the clear supernatant in a separate micro-

fuge tube

l Use 1.5 ul of this clear lysate as template for the PCR

reaction.

Guidelines for PCR Setupl Add 47.5 ml 1X Fastract Tissue cell Direct PCR mix in a flat

capped PCR tube

l Add 0.5 ml of each primers (from 10 mM stock) to the master

mix.

l Use1. 5 ml of Fastract cultured cell lysate as template for the

PCR reaction.

l While setting up the PCR cycle keep the initial denaturation o 0time to be 5 mins at 95 C followed by denaturation at 95 C

for 10 -15s and annealing at the desired Tm. For extension,

use 1min for 1 Kb amplicon size.

Troubleshootingl In case of no PCR reaction dilute the tissue lysate 1:10 or

1:50 with Fastract Tissue lysis buffer and use 1.5 ml of this

dilution as template

l In case of non-specific amplification, increase the

annealing temperature, reduce the total number o f

cycles or design a new set of primers.

Important Notesü Carefully mix and spin down all tubes before opening to

ensure homogeneity and improve recovery. The PCR setup

can be performed at room temperature.

ü Always add the Tissue sample lysate last to the reaction.

ü Always ensure that the volume of lysate that is being added

to the PCR reaction is 3% of the total reaction volume. Do

not add more lysate to the reaction mix.

Ordering Information

Fastract Direct PCR Kit for Forensic Samples

Key Components

Fastract Tissue Lysis Buffer

Fastract Tissue 1X PCR Mix (Fastract DNA Polymerase, Reaction buffer, dNTP (10 mM)) Control Primer Mix (10 mM each)

Cat. # Product Pack Size

G45316AFastract Direct PCR Kit® for

Forensic Samples100 rxn

G45316Fastract Direct PCR Kit® for

Forensic Samples500 rxn

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DescriptionMolecular markers are now widely used to track loci and

genome regions in several crop-breeding programmes. PCR-

based markers analysis and gene expression studies are

increasing exponentially. In these studies high-quality and intact

DNA is the keystone for performing PCR based research.

However, individual species of plants can behave differently

during extraction of due to difference in metabolic activities.

Rice (Oryza sativa L.) synthesize a wide spectrum of

polysaccharides and polyphenols including flavonoids and

other secondary metabolites which interfere with the extraction

of pure genomic DNA. These polysaccharides co-precipitate

during DNA extraction and also known to inhibit polymerase

activity

Fastract Rice Direct PCR Kit provides ease for direct PCR from

Rice leaves without isolation of total genomic DNA. It is an

improved version of GCC Biotech Fastract Plant Direct PCR Kit.

This newly improved and optimized version of Fastract Direct

PCR Kit has been validated over different varieties of rice and

analyzed using 50 SSR genetic markers, distributed across 12

rice chromosomes reported in GRAMENE website

http://archive.gramene.org/species/oryza/rice_intro.html

Simple sequence repeat (SSR) is one of the most robust marker

for identifying rice varieties for assessment of genetic diversity

and population structure.

OhighlightDirect PCR—sample is added directly to PCR reactions therefore

there is no need for time-consuming and expensive DNA

purification steps.

Storage protocol available—allows multiple PCR reactions from

one tiny sample and allows re-testing

Spec i a l l y eng inee red H i F ide l i t y Fa s t r a c t™ DNA

polymerase—extremely short PCR protocol times

·Master Mix format with premixed gel loading dye—minimizes

possibility of cross-contamination, reduces sample handling

and allows direct loading on gel

Marker

Name

Chrom

osome

No

Volume

of Lysis

Buffer

(ml)

Incubati

on Time

PCR

Cycles

Optimi

zed Tm

(⁰C)

Amplicon

size (base

pairs)

Type of

Amplification

Rm 495

Rm 1

Rm 283

Rm 259

Rm 312

Rm 5

Rm 237

Rm 431

Rm 154

Rm 452

Rm 489

OSR 13

Rm 338

Rm 55

Rm 514

Rm 307

Rm 124

Rm 507

Rm 413

Rm 161

Rm 178

Rm 334

Rm 133

Rm 510

Rm 454

Rm 162

Rm 125

Rm 11

Rm 455

Rm 118

Rm 408

Rm 152

Rm 25

Rm 44

Rm 284

Rm 433

Rm 447

Rm 316

Rm 105

Rm 215

Rm 474

Rm 271

Rm 171

Rm 484

Rm 552

Rm 536

Rm 287

Rm 144

Rm 19

Rm 277

1

1

1

1

1

1

1

1

2

2

3

3

3

3

3

4

4

5

5

5

5

5

6

6

6

6

7

7

7

7

8

8

8

8

8

8

8

9

9

9

10

10

10

10

11

11

11

11

12

12

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

30

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

15 Mins

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

40

54

52

58

54

50

54

58

58

66

52

52

58

60

54

56

54

64

56

54

62

62

58

64

58

58

65

65

56

52

62

58

52

56

48

60

58

60

56

65

58

56

50

58

58

56

56

50

64

52

54

159

78

156

172

>500

113

133

251

183

209

271

99

184

>500

245

129

271

258

79

>500

117

>500

230

122

>500

229

>500

126

131

159

129

152

141

>500

148

312

111

197

134

150

252

>500

329

299

195

243

107

225

>500

117

Intense

Moderate

Intense

Intense

Moderate

Moderate

Intense

Intense

Intense

Intense

Intense

Moderate

Moderate

Intense

Intense

Faint

Intense

Intense

Moderate

Faint

Intense

Faint

Intense

Intense

Moderate

Intense

Moderate

Intense

Intense

Intense

Intense

Intense

Faint

Faint

Intense

Intense

Intense

Intense

Moderate

Intense

Faint

Moderate

Intense

Moderate

Faint

Intense

Faint

Intense

Moderate

Intense

Fastract Direct PCR Kit for Rice

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Page 40: MOLECULAR BIOLOGYgccbiotech.co/Catalogue/Catalogue 18-19/Molecular Biology...MOLECULAR Biology DNA template (cDNA/gDNA) Primers/oligos G9 Taq DNA polymerase Hi- G9 Taq DNA polymerase

DescriptionqPCR is the technique used as a standard now a days for assay of

gene expression, copy number determination, disease diagnosis

and several other application. GCC Biotech has developed qPCR

mastermix using all the endogenous reagents and formulations,

starting from the thermostable polymerase and its buffer,

fluorescent dye or the passive reference dye.

Detection Capability as low as 1 copy

of target 10 fold serial dilution of human genomic DNA was used as

template for amplification with b-actin specific primer.

Sensitivity was detected up to 1 copy (2.5pg) of template

(amplification plot). Amplification on serial dilution of template

shows linearity of amplification on wide range of target quantity

(Standard curve). Melting curve shows no nonspecific

amplification.

Fig.1.

Amplification of different target Same amount of A.DNA was

used to amplify different targer regions. Amplification plot

shows different amplification efficiency of the target regions.

Melt curve shows different amplified products.

Detect Wide Range of Target from

Same TemplateAmplification of different target: Same amount of genomic

DNA was used to amplify different target regions. Amplification

plot shows different amplification efficiency of the target

regions. Melt curve shows different amplified products.

Fig.2.

Detection capability as low as 1copy of target Detection

capability as low as 1copy of target: 10 fold serial dilution of

human genomic DNAwas used as template for amplification

with (3-actin specific primer. Sensitivity was detected upto

1copy (2.5pg) of template (amplification plot). Amplification

on serial dilution of template shows linearity of amplification

on wide range of target (Standard curve). Melting curve shows

no non specific amplification.

Featureæ Exceptional sensitivity and specificity: powered by deltaQ,

a cold sensitive PCR enzyme that gives exceptional natural

hot-start activity, and optimized buffer formulation to

eliminate the possibility of primer-dimer formation and/or

non-specific amplification up to a stringent level.

æ Out performs any big brand in this product range:

Provides at least 10 fold more lower limit of detection

while comparing with Thermo or Applied Biosystems

qPCR master mixes.

æ Reproducible and repeatable: The C value over a broad

T dynamic range consistently provides reproducible data.

æ Superior master-mix: Produces excellent linear data with 2accur ate regression value R ~ 0.99), slope and PCR

efficiency (close to 100%) with best compatible

templateprimer

æ Compatible with broad range of Real-Time PCR

instruments: Works with any real-time PCR instrument.

æ Choose from two formulations: contains the ROX

reference dye in separate vial for your optimization to

choose the master mix with or without ROX.

Shipping and StorageqPCR master mix is shipped on dry ice. Upon arrival, store

the componentsat -20°C.

Amplification of same target on

different templategDNA was isolated from 200ml blood of 8 individuals. Equal

amount of DNA (1ng) was used as template for each

amplification. Variation on amplification plot shows variability

of genome and single peak on Melt curve shows single

amplification irrespective of the DNA source.

qPCR Master Mix

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Fig.3.

Amplification of same target on different template gDNA was

isolated from 200�ml blood of 8 individuals. Equal amount of

DNA (1ng) was used as template for each amplification.

Variation on amplification plot shows variability of genome

and single peak on Melt curve shows single amplification

irrespective of the DNA source.

Ordering Information

Get 10-fold higher sensitivity than

the leading master mix

Fig.4.

Comparative analysis with other vendors 400 fg of DNA was

used to amplify a 300 bp fragment using 2X qPCR mix(GCR-51)

and 2x H-eff qPCR mix(GCR-61). Comparative analysis with same

amount of target-primer combination showed higher Ct value

with Thermo 2X DyNAmo Color Flash (F-416) and I- Power SYBR

Green PCR Master Mix (4367659) (amplification plots and Ct

value bar diagram). Melt curve shows specific single product

with GCC 2x qPCR mix and GCC 2x qPCR H-eff mix.

Cat # Product Pack Size

GCR-51 qPCR Master mix, SYBR Rox 1 ml

GCR-52 qPCR Master mix, SYBR Rox 5X1 ml

GCR-53 qPCR Master mix, SYBR Rox 10X1 ml

GCR-54 qPCR Master mix, SYBR Rox 5X5 ml

qPCR Master Mix

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