Microalbumin - Wako .Wao ianostics • Extensive linear range (10-500 ¼g/mL)...

Microalbumin - Wako .Wao ianostics • Extensive linear range (10-500 ¼g/mL) • No significant
Microalbumin - Wako .Wao ianostics • Extensive linear range (10-500 ¼g/mL) • No significant
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Transcript of Microalbumin - Wako .Wao ianostics • Extensive linear range (10-500 ¼g/mL)...

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    Wako Diagnostics

    Extensive linear range (10-500 g/mL)

    No significant effects from common interferents

    Simple, fast, ready to use reagents

    Because microalbuminuria may be reversible if diabetes is well controlled, its early detection may be very beneficial. However, microalbuminuria is not generally measurable by the simple test ordinarily used to detect proteinuria because it lacks sensitivity. For the detection of microalbuminuria, RIA, ELISA and nephelometry assay are used. However they have complicated procedures and are time consuming.

    Wakos Microalbumin is a turbidimetric immunoassay using polyclonal antibodies against human albumin and allows accurate and reproducible measurement of microalbuminuria with a simple procedure.

    Catalog No. Product Name Pkg Size Storage

    999-06001* Autokit Micro Albumin

    Buffer 2 x 45 mL 2-10C

    Antibody 1 x 15 mL 2-10C

    995-06101* Autokit Micro Albumin Calibrator Set 5 conc x 2mL 2-10C

    991-06201* Autokit Micro Albumin Control Set 2 x 2 conc x 2 mL 2-10C* Product is available as a special purchase only.

    Turbidimetric Immunoassay

    MicroalbuminFor In Vitro Diagnostic Use

  • Copyright 2017 Wako Life Sciences, Inc. Wako Life Sciences, Inc. 1025 Terra Bella Ave. Mountain View, CA 94043

    E: diagnostics@wakousa.com T: 650.210.9153 www.wakodiagnostics.com

    Performance characteristics

    PrincipleWhen a sample is mixed with the antibody solution in the buffer, albumin in the sample combines specifically with the anti-human albumin antibody to yield immune complexes resulting in increased turbidity. Since the degree of turbidity is proportional to the amount of albumin in the sample, the amount of albumin can be determined by measuring the turbidity.

    Correlation

    Prozone Phenomenon

    WLS-1952-2

    PrecisionWithin-run precision

    Sample # Replicates Mean (g/mL) SD CV (%)

    1 21 4.97 0.09 1.81

    2 21 105.81 0.79 0.75

    3 21 510.84 3.91 0.77

    Total precisionNumber of assay days

    Mean (g/mL) SD CV (%) SWT ST

    21 4.90 0.14 2.86 0.23 0.23

    21 511.21 7.68 1.50 7.65 9.78

    AccuracyThe accuracy of this method was demonstrated by a recovery study.

    No. Expected value (g/mL)Obtained value

    (g/mL)Recovery

    (%)

    1 33.1 32.6 98.4

    2 58.1 56.9 97.8

    3 118.1 117.6 99.6

    0 100 200 300 400 500 6000

    100

    200

    300

    400

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    600

    y = 1.0179x - 0.9619r = 0.9992

    Wak

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    Wako Micro Albumin B, g/mL

    0 5000 10000 15000 20000 25000 30000 350000

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    Obt

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    Micro Albumin expected conc., g/mL

    Prozone phenomenon not observed up to 10000 g/mL

    InstrumentsVarious automated analyzer applications are available.

    For more product information, please contact customer service at 1-877-714-1924.