Microalbumin - Wako Diagnostics · Wao ianostics • Extensive linear range (10-500 μg/mL) • No...
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www.wakodiagnostics.com Wako Diagnostics • Extensive linear range (10-500 μg/mL) • No significant effects from common interferents • Simple, fast, ready to use reagents Because microalbuminuria may be reversible if diabetes is well controlled, its early detection may be very beneficial. However, microalbuminuria is not generally measurable by the simple test ordinarily used to detect proteinuria because it lacks sensitivity. For the detection of microalbuminuria, RIA, ELISA and nephelometry assay are used. However they have complicated procedures and are time consuming. Wako’s Microalbumin is a turbidimetric immunoassay using polyclonal antibodies against human albumin and allows accurate and reproducible measurement of microalbuminuria with a simple procedure. Catalog No. Product Name Pkg Size Storage 999-06001* Autokit Micro Albumin Buffer 2 x 45 mL 2-10°C Antibody 1 x 15 mL 2-10°C 995-06101* Autokit Micro Albumin Calibrator Set 5 conc x 2mL 2-10°C 991-06201* Autokit Micro Albumin Control Set 2 x 2 conc x 2 mL 2-10°C * Product is available as a special purchase only. Turbidimetric Immunoassay Microalbumin For In Vitro Diagnostic Use
Transcript of Microalbumin - Wako Diagnostics · Wao ianostics • Extensive linear range (10-500 μg/mL) • No...
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Wako Diagnostics
• Extensive linear range (10-500 μg/mL)
• No significant effects from common interferents
• Simple, fast, ready to use reagents
Because microalbuminuria may be reversible if diabetes is well controlled, its early detection may be very beneficial. However, microalbuminuria is not generally measurable by the simple test ordinarily used to detect proteinuria because it lacks sensitivity. For the detection of microalbuminuria, RIA, ELISA and nephelometry assay are used. However they have complicated procedures and are time consuming.
Wako’s Microalbumin is a turbidimetric immunoassay using polyclonal antibodies against human albumin and allows accurate and reproducible measurement of microalbuminuria with a simple procedure.
Catalog No. Product Name Pkg Size Storage
999-06001* Autokit Micro Albumin
Buffer 2 x 45 mL 2-10°C
Antibody 1 x 15 mL 2-10°C
995-06101* Autokit Micro Albumin Calibrator Set 5 conc x 2mL 2-10°C
991-06201* Autokit Micro Albumin Control Set 2 x 2 conc x 2 mL 2-10°C* Product is available as a special purchase only.
Turbidimetric Immunoassay
MicroalbuminFor In Vitro Diagnostic Use
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E: [email protected] T: 650.210.9153 www.wakodiagnostics.com
Performance characteristics
PrincipleWhen a sample is mixed with the antibody solution in the buffer, albumin in the sample combines specifically with the anti-human albumin antibody to yield immune complexes resulting in increased turbidity. Since the degree of turbidity is proportional to the amount of albumin in the sample, the amount of albumin can be determined by measuring the turbidity.
Correlation
Prozone Phenomenon
WLS-1952-2
PrecisionWithin-run precision
Sample # Replicates Mean (μg/mL) SD CV (%)
1 21 4.97 0.09 1.81
2 21 105.81 0.79 0.75
3 21 510.84 3.91 0.77
Total precisionNumber of assay days
Mean (μg/mL) SD CV (%) SWT ST
21 4.90 0.14 2.86 0.23 0.23
21 511.21 7.68 1.50 7.65 9.78
AccuracyThe accuracy of this method was demonstrated by a recovery study.
No. Expected value (μg/mL)
Obtained value (μg/mL)
Recovery (%)
1 33.1 32.6 98.4
2 58.1 56.9 97.8
3 118.1 117.6 99.6
0 100 200 300 400 500 6000
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y = 1.0179x - 0.9619r = 0.9992
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Micro Albumin expected conc., μg/mL
Prozone phenomenon not observed up to 10000 μg/mL
InstrumentsVarious automated analyzer applications are available.
For more product information, please contact customer service at 1-877-714-1924.
