Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

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Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in Novel Advanced Materials and Processing with Applications in Biomedical, Electrical and Chemical Engineering

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Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices. Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in - PowerPoint PPT Presentation

Transcript of Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

Page 1: Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

Author: Gene Yu Co-Authors: Dr. AlexBlake

Dr. David EddingtonJuly 29, 2010

NSF Research Experiences for Undergraduates (REU) inNovel Advanced Materials and Processing with Applications in Biomedical, Electrical and

Chemical Engineering

Page 2: Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

ObjectiveDesign localized and

automated delivery system for microfluidic brain slice devices (μBSDs)

Delivers chemicals/drugs and gases

Automatically mixes oxygen

Characterize delivery of chemicals through tissue Image taken from

Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.

Page 3: Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

MotivationμBSDs allow in vitro study of:

NeurogenesisSynaptogenesisRegenerationProtein expressionResponses to physical trauma

Image taken fromhttp://www.koki.hu/cdnb/research/research.html

Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.

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RelevanceChemical Delivery

Spatial resolution Current systems affect entire

slice with drug Desire stimulation of specific

areas

Oxygen DeliveryAutomatic Mixing

Saves money Pre-mixing is not required

AutomationMulti-tasking

Image modified fromhttp://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp

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Oxygen Mixing and DeliveryExperimental Set-Up

Y Connector

Output Tube

Valves Gas Feed Lines: 0% and 21%

Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor

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Oxygen Mixing and DeliveryOutput

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Oxygen Mixing and DeliveryGraphical User Interface (GUI)

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Delivery CharacterizationμBSD Design

T Channel

Delivery Channel

Reservoir

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Delivery CharacterizationExperimental Set-up

μBSD

Valve

DI Water Lines T Channel

Vacuum Line

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Delivery CharacterizationNo Tissue Delivery

10ms 15ms 20ms 25ms

2x5ms 3x5ms 4x5ms 5x5ms

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Delivery CharacterizationLinear Relationships: No Tissue

Width (µm)

Width (µm)

860

1320

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Delivery CharacterizationLinear Relationships: With Tissue

Full Dose Chemical Delivery

Pulsing Dose Chemical DeliveryMaximum Intensities

0 2 4 6Number of 5ms Pulses

Inte

nsi

ty

Inte

nsi

tyIn

ten

sity

Time (min)

Time (min)

2

2

Maximum Intensities

0 10 20

30Valve Open Time (ms)

Inte

nsi

ty

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Delivery CharacterizationGUI

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ConclusionsOxygen Delivery

Precise and accurate results

Reliable oxygen switching

Chemical Delivery Demonstrated linear

relationship Area of effect: ~2mm x

2mm Selective and

independent valve control

Page 15: Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

AcknowledgementsFunding

NSF-REU DoD-ASSURE EEC-NSF Grant # 0755115

REU Directors Christos G. Takoudis, Ph.D. Greg Jursich, Ph.D.

Research Advisor David Thomas Eddington, Ph.D.

Mentors Alexander Blake, Ph.D. Gerardo Mauleon