Lab Exercise 14:
-
Author
justin-rojas -
Category
Documents
-
view
35 -
download
1
Embed Size (px)
description
Transcript of Lab Exercise 14:
-
Lab Exercise 14: Biochemical Differentiation of some Medically Important Gram Positive Cocci
-
Beta ( ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin)
-
Alpha () hemolysis (blood agar around bacterial colonies looks greenish-brown, indicating partial breakdown and consumption of hemoglobin)Mixed culture from a throat swab; note several -hemolytic colonies
-
Gamma () hemolysis (really means no hemolysis; blood agar around colonies looks normal)
-
Side-by-side comparisons of alpha (), beta (), and gamma () hemolysis
-
Bacitracin (Taxo A) sensitivity + results with a zone of inhibition
-
Coagulase Test+-
-
Carbohydrate Fermentation Tests (Glucose and Mannitol)+- (same as uninoculated media)Phenol red indicator turns yellow at acid pH
-
Catalase Test
-
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Streptococcus pyogenes
-
G MUse the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Micrococcus lutea
-
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!AAnswer: Enterococcus faecalis
-
Answer: Staphylococcus aureus
-
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Staphylococcus epidermidis
-
Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Streptococcus pneumoniae
-
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!AAnswer: Streptococcus mutans
********************