Lab Exercise 14:

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Lab Exercise 14:. Biochemical Differentiation of some Medically Important Gram Positive Cocci. Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin). - PowerPoint PPT Presentation

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  • Lab Exercise 14: Biochemical Differentiation of some Medically Important Gram Positive Cocci

  • Beta ( ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin)

  • Alpha () hemolysis (blood agar around bacterial colonies looks greenish-brown, indicating partial breakdown and consumption of hemoglobin)Mixed culture from a throat swab; note several -hemolytic colonies

  • Gamma () hemolysis (really means no hemolysis; blood agar around colonies looks normal)

  • Side-by-side comparisons of alpha (), beta (), and gamma () hemolysis

  • Bacitracin (Taxo A) sensitivity + results with a zone of inhibition

  • Coagulase Test+-

  • Carbohydrate Fermentation Tests (Glucose and Mannitol)+- (same as uninoculated media)Phenol red indicator turns yellow at acid pH

  • Catalase Test

  • Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Streptococcus pyogenes

  • G MUse the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Micrococcus lutea

  • Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!AAnswer: Enterococcus faecalis

  • Answer: Staphylococcus aureus

  • Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Staphylococcus epidermidis

  • Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!Answer: Streptococcus pneumoniae

  • Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!AAnswer: Streptococcus mutans

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