ISO 6579 culture media - Laboratorios Conda · Peptones / Salts / Buffer BUFFERED PEPTONE WATER....

Culture media

ISO 6579

Culture Media in ISO 6579

MKTTn BrothCat. 1173

Biochemical Test

Buffered Peptone WaterCat. 1402

RVS BrothCat. 1174

XLD Agar Cat.1274 & SalmonellaChromogenic Agar Cat.1122

Nutrient AgarCat. 1060

Urea

Indol

TSI Agar Cat. 1172

β Galactosidase

Lisine DecarboxilaseBroth Cat. 1176

Voges-Proskauer

Salmonella



Biochemical Test


RVS BrothCat. 1174



Medium richin nutrientsMedium richin nutrients

Allows the detection of smallnumber of Salmonella or injured

Salmonella

Incubate at 37±1°C during 18 ± 2 h

Peptones / Salts / Buffer

BUFFERED PEPTONE WATER



Biochemical Test


RVS BrothCat. 1174



Peptone / Salts / BufferMagnesium chloride /

Malaquite green

RVS Broth (Rappaport Soja acc VASSILIADIS)

After incubation there isa lot of Salmonella cells

0.1 ml of the incubated buffered peptonewater is transfered into 10 ml of RVS Broth.

Incubate at 41.5±1°C during 24±2 hours

0.1 ml of the incubated buffered peptonewater is transfered into 10 ml of RVS Broth.

Incubate at 41.5±1°C during 24±2 hours

Malaquite green inhibitsthe growth of

accompanying flora

Magnesium cloride allowsa better growth of

Salmonella



Biochemical Test


RVS BrothCat. 1174



Peptone / Salts / BufferCalcium carbonate / Tetrationate /

Ox bile / Brilliant Green / Novobiocine

MKTTn Broth

After incubation there are a lot ofSalmonella cells

1 ml of the incubated buffered peptone wateris transfered into 10 ml tube of MKTTn Broth.

Incubate at 37±1°C during 24±2 hours

1 ml of the incubated buffered peptone wateris transfered into 10 ml tube of MKTTn Broth.

Incubate at 37±1°C during 24±2 hours

Tetrationate inhibits thegrowth of intestinal

bacteria

Bile stimulate Salmonellagrowing and inhibitsaccompanying flora

Brilliant green preventsthe growth of Gramnegative bacteria

Novobiocine inhibits theaccompanying flora



Biochemical Test


RVS Broth Cat. 1174

XLD Agar Cat.1274 & SalmonellaChromogenic Cat.1122


Peptones / Salts / BufferXilose, Lactose, Sucrose & Phenol Red / Sodium Thiosulfate y Ferric Ammonium

Citrate / L-lysine

XLD Agar (Xilose LisineDesoxicholate)

Incubate at 37±1°C during 24±3 hoursIncubate at 37±1°C during 24±3 hours

Salmonella growth in blackcentered red colonies

If Xilose, lactose and sucrose are fermented,

the pH decrease, changing the color of

phenol red, that is a pH indicator

If Xilose, lactose and sucrose are fermented,

the pH decrease, changing the color of

phenol red, that is a pH indicator

Sodium Thiosulfateprovides Sulphur and

Ferric ammonium citrate is the indicator

for H2S production

Sodium Thiosulfateprovides Sulphur and

Ferric ammonium citrate is the indicator

for H2S production

Lisine is decarboxilated, producing cadaverine, which increases the pH and forming a purple halo around the colony

Lisine is decarboxilated, producing cadaverine, which increases the pH and forming a purple halo around the colony



Biochemical Test

Buffered Peptone waterCat. 1402

RVS BrothCat. 1174



Peptones / Salts / Chromogenic Substrate

SALMONELLA CHROMOGENIC AGAR

Incubate at 35±1°C during 18-24 hours


Salmonella growth in magenta colonies

Esterase activitycleaves one of the

chromogenicsubstrates, giving the

colony a magenta color

Esterase activitycleaves one of the

chromogenicsubstrates, giving the

colony a magenta color

β-Galactosidaseactivity cleaves theother chromogenicsubstrate, giving thecolony a green color

β-Galactosidaseactivity cleaves theother chromogenicsubstrate, giving thecolony a green color



Biochemical Test


RVS BrothCat. 1174



Peptone / Salts / Buffer

NUTRIENT AGAR



Medium rich in nutrients

Medium rich in nutrients

Isolated colonies forbiochemical tests

Isolated colonies forbiochemical tests



Biochemical Test


RVS BrothCat. 1174



TSI AGAR



Glucose fermentationturns the medium into

yellow and may product gas


yellow and may product gas

Lactose andSucrose

fermentation turnsthe medium into red

Lactose andSucrose

fermentation turnsthe medium into red

Sodium Thiosulfate provides Sulphur and Ferric Ammonium

citrate, which is the indicator for H2S production

Sodium Thiosulfate provides Sulphur and Ferric Ammonium

citrate, which is the indicator for H2S production

Salt / BufferGlucose / Lactose & Sucrose

Phenol Red / Ferric ammonium citrate& Sodium thiosulfate



Biochemical Test


RVS BrothCat. 1174



Glucose / Bromocresol Purple /

Lisine

LYSINE DECARBOXYLASE BROTH

Incubate at 37±1°C during24 ± 3 hours

Incubate at 37±1°C during24 ± 3 hours


yellow


yellow

Lisine is decarboxilated,producing cadaverine, whichincreases the pH and forms

a purple halo aroundthe colony

Lisine is decarboxilated,producing cadaverine, whichincreases the pH and forms

a purple halo aroundthe colony

Tubes with a positive reaction to lisine

decarboxilation will presenta purple color

Tubes with a positive reaction to lisine

decarboxilation will presenta purple color

Results

ISO 6579 culture media - Laboratorios Conda · Peptones / Salts / Buffer BUFFERED PEPTONE WATER....

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