Impact of feed supplementation with different omega 3 rich

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IMPACT OF FEED SUPPLEMENTATION WITH DIFFERENT OMEGA-3 RICH MICROOALGAE SPECIES ON ENRICHMENT OF EGGS OF LAYING HENS Charlotte Lemahieu, Charlotte Bruneel, Romina Termote-Verhalle, Koenraad Muylaert, Johan Buyse, Imogen Foubert

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Transcript of Impact of feed supplementation with different omega 3 rich

Page 1: Impact of feed supplementation with different omega 3 rich

IMPACT OF FEED SUPPLEMENTATION WITH DIFFERENT OMEGA-3 RICH MICROOALGAE SPECIES ON

ENRICHMENT OF EGGS OF LAYING HENS

Charlotte Lemahieu, Charlotte Bruneel, Romina Termote-Verhalle, Koenraad

Muylaert, Johan Buyse, Imogen Foubert

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I. INTRODUCTION

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Omega-3

α-Linolenic acid (ALA), 18:3 (n-3)

Eicosapenttaenoic acid (EPA), 20:5 (n-3)

Docosahexaenoic acid (DHA), 22:6 (n-3)

Stearidonic acid (SDA), 18:4 (n-3)

Docosapentaenoic acid (DPA), 22:5 (n-3)

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Reduction and prevention of several cardiovascular diseases

Prevention and treatment of several chronic diseases Maternal intake of n-3 LC-PUFA lowers the risk of

preterm birth and low birth weight DHA is critical to foetal growth and development of

visual and cognitive functions in foetus and children Daily intake of n-3 LC-PUFA from 140 to 667 mg/day But!!! A few countries reach the intake of 250 mg n-3 LC-

PUFA per day => Enrich food products with n-3 LC-PUFA has emerged

Why do we supplement Omega-3 Fatty Acids in egg?

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Flaxseed gives mainly rise to enrichment of ALA in the egg yolk (Fraeye et al., 2012) quite inefficient conversion of ALA to EPA and DHA

in laying hens Fish oil provides an enrichment of EPA and

especially DHA in the egg yolk (Van Elswyk, 1997) The declining fish stocks, the presence of pollutants

(heavy metals and PCBs) in fish ≥1.5% fish oil => The off-flavours detected in eggs

Alternative sources of n-3 LC-PUFA must be found

Related researchers

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Nannochloropsis was supplemented to laying hens’ feed to raise the level of n-3 LC-PUFA in the egg yolk Fatty acid profile change considerably as well as

redness of the egg yolk increased (Bruneel et al., 2013; Fraeye et al., 2012; Fredriksson et al., 2006; Nitsan et al., 1999)

Chlorella and Porphyridium, gave rise to a transfer of carotenoids from the microalgae to the egg yolk (Ginzberg et al., 2000; Gouveia et al., 1996)

Phaeodactylum, as the most promising EPA source and Isochrysis, as the most promising DHA source, were recommended (Bruneel et al., 2013)

About microalgal researchers

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Investigate the influence of using four different autotrophic microalgae for n-3 LC-PUFA enrichment in eggs of laying hens. Phaeodactylum tricornutum, Isochrysis galbana,

Nannochloropsis oculata and Chlorella fusca

The four autotrophic microalgae were supplemented in two different concentrations to the laying hens’ feed

The fatty acid profile, the carotenoid and sterol content, and the colour of egg yolk were measured

Objective

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II. MATERIALS AND METHODS

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Commercial products: Phaeodactylum tricornutum (freeze dried biomass,

SBAE, Belgium) Nannochloropsis oculata (freeze dried biomass, SBAE,

Belgium) Isochrysis galbana (freeze dried biomass, Algaenergy,

Spain) Chlorella fusca (drum dried biomass, Ingrepro, The

Netherlands)

The proximate composition (total lipids, n-3 PUFA, carotenoids and sterols) and the moisture content was determined

2.1. Microalgal biomass

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72 ISA Brown laying hens 29 weeks of age two hens/cage temperature 200C 16 h of light per day ad libitum

The experimental trial lasted 42 days 14 days of adaptation 28 days of

supplementation with microalgae

2.2. Animals, housing and diets

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2.2. Animals, housing and diets (cont.)

No. Treatments Diets

1 Control Without supplement

2 Phaeodactylum triconutum 0,125%

3 Phaeodactylum triconutum 0,25%

4 Nannochloropsis oculata 0,125%

5 Nannochloropsis oculata 0,25%

6 Isochrysis galbana 0,125%

7 Isochrysis galbana 0,25%

8 Chlorella fusca 0,125%

9 Chlorella fusca 0,25%

After the adaptation period, the hens were randomly assigned to one of the nine treatment diets (n=8 hens per treatment diet)

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Feed intake Egg production Mortality and morbidity

Body weight (day 0 and day 28)

2.3. Zootechnical performance of laying hens

were registered on daily basis during the entire trial of 42 days

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Eggs were collected daily and stored at -200C until further analysis. The eggs at the end of the supplementation period were analysed/ or determined for: Egg quality parameters Total lipid content The content and composition of n-3 PUFA,

carotenoids and sterols

To calculate the enrichment of n-3 LC-PUFA in eggs by dietary supplementation of microalgae, the fatty acid profile of the eggs at the start (day 0) of the supplementation period was also analysed.

2.4. Sample collection and storage of eggs

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Results were statistically evaluated by two-way analysis of variance (ANOVA) and a post hoc Tukey’s test with α= 0.05 (Sigmaplot 11, Systat Software, Inc., Illinois, USA).

2.5. Statistical analysis

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III. RESULT AND DISCUSSION

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Table 1. Proximate composition of four microalgae: the lipid content (g/100 g DM), n-3 PUFA content (g/100 g biomass), carotenoid content (µg/gDM) and sterol content (mg/g DM)

3.1. Proximate composition of n-3 PUFA rich autotrophic microalgae

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The average daily feed intake, egg production, mortality and morbidity were not significantly affected by supplementation of microalgae to the laying hens.

The body weight of the laying hens remained constant, except for hens supplemented with Nannochloropsis (in both doses), which displayed a slight decrease (<5%) in body weight.

3.2. Zootechnical performance of laying hens

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Table 2. Egg quality parameters at the end of microalgal supplementation period: egg weight, shell weight, albumen weight, yolk weight (g), shell thickness (mm) and shell strength (N/m)

3.3. Egg parameters

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Fig.1. Level of omega-3 fatty acids (mg/egg) in eggs measured at the end of the supplementation period with four microalgae supplemented in 2 doses (0.125% and 0.25%); control (0%)

3.4. Enrichment of egg yolk 3.4.1. n-3 PUFA enrichment

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Table 3. The supplemented n-3 PUFA (mg/100 g feed), the enrichment of n-3 LC-PUFA (mg/egg) and the conversion efficiency of n-3 LC-PUFA (%) for the four microalgae supplemented in 2 doses (0.125% and 0.25%)

3.4. Enrichment of egg yolk (cont.)3.4.1. n-3 PUFA enrichment

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Table 4. Colour values (Roche and CIELAB-value) of the egg yolk measured at the end of the supplementation period with four microalgae supplemented in 2 doses (0.125% and 0.25%)

3.4. Enrichment of egg yolk (cont.)3.4.2. Yolk colour and carotenoid enrichment

L*: lightnessa*: red-greenessb*: yellow-blueness

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As microalgae contain significant levels of health beneficial phytosterols (Table 1) may be transferred to the egg yolk in a similar way as

n-3 LC-PUFA and carotenoids may decrease the amount of cholesterol in the egg

yolk, giving the cholesterol lowering properties of phytosterols

No phytosterols were transferred from the microalgae to the egg yolk

Cholesterol was detected in the egg yolk=> Reducing the cholesterol content in egg yolk is rather impossible

3.4. Enrichment of egg yolk (cont.)3.4.3. Yolk sterol enrichment

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IV. CONCLUSION

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All four microalgae gave rise to increased levels of n-3 LC-PUFA in the egg yolk

The highest enrichment of n-3 LC-PUFA, DHA, was achieved by supplementation of Phaeodactylum and Isochrysis; a lower enrichment, Nannochloropsis; and Chlorella gave rise to the lowest enrichment

Increase of the carotenoid content was detected by supplementation of Phaeodactylum, Isochrysis and Nannochloropsis

The sterol content of the egg yolk was hardly influenced

Omega-3 rich autotrophic microalgae offer an alternative to current sources of n-3 LC-PUFA to increase the level of these fatty acids in the eggs.

The carotenoid enrichment should be taken into account since the colour shift of the egg yolk could decrease the consumers’ acceptability.

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Thank you for your attention!