Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç...

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Διαγονιδιακά ζώα

Transcript of Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç...

Page 1: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov

Διαγονιδιακάζώα

Page 2: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov

Transgene DNA

Analyse the progeny by PCR or Southern blotting

Παραγωγή διαγονιδιακών ζώων μεμικροένεση στο προπυρήνα

Page 3: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 4: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 5: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 6: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 7: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 8: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 9: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
Page 10: Human genetics 9 - FORTH-IMBB · Rosa26 x P (gene of interest)-Cre X-gal ... GCA GCA .KaMlépYElEç KUTTáPWV enÅa0TlK(jv AvTlŒrpot.pó (loxP at avTí6tTn t.popá ) EKtppaon: (a-rov
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Xgal (turns blue when cleaved by b-gal)

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Ιστοειδικός έλεγχος τηςέκφρασης της

Cre ρεκομπινάσης

Rosa26 x P (gene of interest)-Cre

X-gal

B-galactosidase activity

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Ζωικά μοντέλα για τη κατανόηση συνδρόμων:

Csbm/m/Xpa-/-XPDTTD Ercc1-/-

TTD Cockayne XFE

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Ιστοειδική παθολογία (ένας τύπος ιστού/κυττάρων φέρει την αλλοίωση)

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Στο Tet-On σύστημα, Η μεταγραφή είναι ενεργή

Παρουσία Dox

Στο Tet-Off σύστημα, Η μεταγραφή είναι ανενεργή

παρουσία Tc ή Dox

Doxocycline

Tetracycline-responsive element

rTA(transactivator protein)

tTA(tetracycline-controlled transactivator protein)

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In vivo σήμανση πρωτεϊνών:In vivo biotinylation tagging of transcription factors

Biotin-TF

MSChIP-on-chip

Σε κυτταρικές σειρές:

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BirABirA TgTg

Χ

BiotinBiotin--tagged TFtagged TF11

BiotinBiotin--tagged TFtagged TF22

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In vivo σήμανση γονιδιακής έκφρασης:

Internal ribosomal entry site

Gene of interest

Cytomegalovirus promoter

Green fluorescent protein

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In vivo σήμανση γονιδιακής έκφρασης:

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Συντήρηση γενετικά τροποποιημένων ζωικών μοντέλων

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Κρυο-συντήρηση εμβρύων

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Measurement of jVcb with 0 decays - arXivMeasurement of jVcbjwith B0 s!D s + decays LHCb collaborationy Abstract The element jV cbjof the Cabibbo{Kobayashi{Maskawa matrix is measured

Measurement of jVcb with 0 decays - arXivMeasurement of jVcbjwith B0 s!D s + decays LHCb collaborationy Abstract The element jV cbjof the Cabibbo{Kobayashi{Maskawa matrix is measured

Κωνσταντίνος Ε. Βοργιάς PhD€¦ · •Διάλεξη στο Hebrew University, Jerusalem, Haddash Medical School. Aug. 1994. • Διάλεξη στο IMBB, Enzyme

Κωνσταντίνος Ε. Βοργιάς PhD€¦ · •Διάλεξη στο Hebrew University, Jerusalem, Haddash Medical School. Aug. 1994. • Διάλεξη στο IMBB, Enzyme

B D Dˇ B D - arXiv · form factors [18{20], resulting in a value for jV cbjcompatible with the inclusive one [11,21,22]. Although the outcome refers to a single data set, the question

B D Dˇ B D - arXiv · form factors [18{20], resulting in a value for jV cbjcompatible with the inclusive one [11,21,22]. Although the outcome refers to a single data set, the question

622816el - European Parliament · !"#$%%&'$()*+,- /p'33 .( /0$*1%1 23'433!" d/g s jisojho kzw qgie\jv^w fgkv]fo]fqj kv]^w syjzw \gv]fvwzwhol nvo kvl ]fvwzwh\l _ kiw misf]eokho ]ov

622816el - European Parliament · !"#$%%&'$()*+,- /p'33 .( /0$*1%1 23'433!" d/g s jisojho kzw qgie\jv^w fgkv]fo]fqj kv]^w syjzw \gv]fvwzwhol nvo kvl ]fvwzwh\l _ kiw misf]eokho ]ov

Circulation of the Disc and Retina · PDF fileCirculation of the Disc and Retina ... • Occult GCA : ... Interferon αlpha – associated Optic Neuropathy

Circulation of the Disc and Retina · PDF fileCirculation of the Disc and Retina ... • Occult GCA : ... Interferon αlpha – associated Optic Neuropathy

Table EA-1. a - ESGxraysweb.lbl.gov/uxas/Publicatons/Papers/pdfs/Lanson GCA roots supp.pdf · 1 mm Fe Zn Mn Fig. EA-2: Tricolor (RGB) μ-XRF map of a bundle of roots. Red codes for

Table EA-1. a - ESGxraysweb.lbl.gov/uxas/Publicatons/Papers/pdfs/Lanson GCA roots supp.pdf · 1 mm Fe Zn Mn Fig. EA-2: Tricolor (RGB) μ-XRF map of a bundle of roots. Red codes for

Supporting Information - PNAS TCC TTT TTA-3′ (sense) and 5′-AGC TTA AAA AGG ATA TGG GCA ATG AAA TTT CTC TTG AAA ATT TCA TTG CCC ATA TCC GGG-3′ (antisense). The oligo sequences

Supporting Information - PNAS TCC TTT TTA-3′ (sense) and 5′-AGC TTA AAA AGG ATA TGG GCA ATG AAA TTT CTC TTG AAA ATT TCA TTG CCC ATA TCC GGG-3′ (antisense). The oligo sequences

Human Genetics 2 - FORTH-IMBB€¦ · syndromes a.o. XP, CS, TTD TTDA. damage recognition excision DNA synthesis + ligation HHR23B XPC XPE DDB2 UV UV RNA pol II CSA XPA XAB2 UVS TFIIH

Human Genetics 2 - FORTH-IMBB€¦ · syndromes a.o. XP, CS, TTD TTDA. damage recognition excision DNA synthesis + ligation HHR23B XPC XPE DDB2 UV UV RNA pol II CSA XPA XAB2 UVS TFIIH

L10 chapter9 1 - FORTH-IMBB€¦ · 1. τα φυτά ήταν ανθεκτικά μόνο στον ιό pvx και όχι σε άλλους ιούς. 2. Και ενώ αναμενόταν

L10 chapter9 1 - FORTH-IMBB€¦ · 1. τα φυτά ήταν ανθεκτικά μόνο στον ιό pvx και όχι σε άλλους ιούς. 2. Και ενώ αναμενόταν

]m¿-´n-]-{X-{]-h¿-ØIs Hm¿--°p-dn-pI · aq-cym-Sv am¸n-f FÂ.]n. kv-¡qÄ, Ip-¯p-]d-¼v t_mÀ-Uv kv-¡qÄ, sXm-¡n-e-§m-Sn l-bÀ sk-¡ï-dn kv-¡qÄ, ^m-dq-Jv tIm-tf-Pv F-¶n-h-S-§fnÂ

]m¿-´n-]-{X-{]-h¿-ØIs Hm¿--°p-dn-pI · aq-cym-Sv am¸n-f FÂ.]n. kv-¡qÄ, Ip-¯p-]d-¼v t_mÀ-Uv kv-¡qÄ, sXm-¡n-e-§m-Sn l-bÀ sk-¡ï-dn kv-¡qÄ, ^m-dq-Jv tIm-tf-Pv F-¶n-h-S-§fnÂ

Small Divisors and the NLSE - University of Washingtonblwilson/MSRI9.4.pdfq >cq r for any p=q 2Q, q >0. Note: Diophantine numbers have full measure. Assuming is Diophantine, jv^(n)j.

Small Divisors and the NLSE - University of Washingtonblwilson/MSRI9.4.pdfq >cq r for any p=q 2Q, q >0. Note: Diophantine numbers have full measure. Assuming is Diophantine, jv^(n)j.

Основные положения · Jv = Lp S [ (Pc - Pi) – σd (7πc - πi) ] Jv – скорость транскапилярной фильтрации (cm3/sec) ... ARDS.

Основные положения · Jv = Lp S [ (Pc - Pi) – σd (7πc - πi) ] Jv – скорость транскапилярной фильтрации (cm3/sec) ... ARDS.